BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0276.0004.p (156 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yvdA [P] COG0288 Carbonic anhydrase 206 1e-53 BS_ytiB [P] COG0288 Carbonic anhydrase 204 6e-53 BS_ybcF [P] COG0288 Carbonic anhydrase 70 2e-12 SSO1214 [P] COG0288 Carbonic anhydrase 69 2e-12 Rv1284 [P] COG0288 Carbonic anhydrase 65 3e-11 >BS_yvdA [P] COG0288 Carbonic anhydrase Length = 197 Score = 206 bits (523), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 90/153 (58%), Positives = 119/153 (77%) Query: 1 MLIITCMDTRLVELLPKAMNFKNGDVKIIKNAGAVISQPFGSVMRSVMVALYELDADEVI 60 ++I+TCMDTRL ELLP+AM KNGD KI+KNAGA++S PFGSVMRS++VA+YEL A+EV Sbjct: 36 LVIVTCMDTRLTELLPQAMGLKNGDAKIVKNAGAIVSHPFGSVMRSILVAIYELQAEEVC 95 Query: 61 VVGHYECGMASLNADRMINSIKERGVSDEVLSTLENSGIKLTKWLRGFDNVEEGVIQTVE 120 +VGH+ECGM+ LNA ++ KERGV D L+ L ++G+ L WL GF +VEE V +V Sbjct: 96 IVGHHECGMSGLNASSILEKAKERGVEDSCLNLLTSAGLDLKTWLTGFHSVEESVSHSVN 155 Query: 121 LIKRHPLLPPNVPIHGMIIDPATGALELVSDGY 153 +IK HPLLP VP+HG++I P TG L++V +GY Sbjct: 156 MIKNHPLLPKKVPVHGLVIHPETGKLDVVINGY 188 >BS_ytiB [P] COG0288 Carbonic anhydrase Length = 187 Score = 204 bits (518), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 85/153 (55%), Positives = 123/153 (80%) Query: 1 MLIITCMDTRLVELLPKAMNFKNGDVKIIKNAGAVISQPFGSVMRSVMVALYELDADEVI 60 M I++CMDTRLVELLP AMN +NGDVKIIK+AGA+++ PFGS+MRS++VA+YEL+ADEV Sbjct: 33 MAILSCMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFGSIMRSILVAVYELNADEVC 92 Query: 61 VVGHYECGMASLNADRMINSIKERGVSDEVLSTLENSGIKLTKWLRGFDNVEEGVIQTVE 120 V+GH++CGM+ +++ M+ IK RG+ +E + T++ SG+ +W + FD+VE V +V+ Sbjct: 93 VIGHHDCGMSKISSKSMLEKIKARGIPEERIETIKYSGVDFDQWFKSFDSVEASVKDSVD 152 Query: 121 LIKRHPLLPPNVPIHGMIIDPATGALELVSDGY 153 +IK HPL P NVP+HG++IDP TG L+L+ +GY Sbjct: 153 VIKHHPLFPENVPVHGLVIDPKTGKLDLIVNGY 185 >BS_ybcF [P] COG0288 Carbonic anhydrase Length = 175 Score = 69.7 bits (169), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 41/147 (27%), Positives = 83/147 (56%), Gaps = 5/147 (3%) Query: 1 MLIITCMDTRLVELLPKAMNFKNGDVKIIKNAGAVISQPFGSVMRSVMVALYELDADEVI 60 +L +T ++ L +L +A N ++ I++ GA IS P+G +MRSV+ A+Y+ D +E+ Sbjct: 9 VLFLTDIENGLEPILQEATNTSAENMLTIQSYGASISHPYGEIMRSVIFAIYQEDVEEIF 68 Query: 61 VVGHYECGMASLNADRMINSIKERGVSDEVL---STLENSGIKLTKWLRGFDNVEEGVIQ 117 VVG + ++ N + ++K++ + + L E G + +WL +N + + + Sbjct: 69 VVGTKDKKTSAGNGLTQLETMKDKIQTLDYLFQNCKPEFLGGTVDEWLN--ENSSDTIEK 126 Query: 118 TVELIKRHPLLPPNVPIHGMIIDPATG 144 +V++I+ HPL+P V + G+ ++ G Sbjct: 127 SVDMIRHHPLVPSYVKVRGLFVNHKGG 153 >SSO1214 [P] COG0288 Carbonic anhydrase Length = 204 Score = 69.3 bits (168), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 43/158 (27%), Positives = 76/158 (48%), Gaps = 17/158 (10%) Query: 1 MLIITCMDTRLVELLPKAMNFKNGDVKIIKNAGAVISQPFGSVMRSVMVALYELDADEVI 60 + I+TCMD R+ + +A+ + D I +NAG +++ +RS + E+I Sbjct: 30 LWILTCMDERV--HVEEALGIRPEDAHIYRNAGGIVTD---DAIRSASLTTNFFGTKEII 84 Query: 61 VVGHYECGMASLNADRMINSIKERGVS------DEVLSTLE-NSGIKLTKWLRGFDNV-- 111 V+ H +CGM D + ++GV D +L +L S TKW + F ++ Sbjct: 85 VITHTDCGMIRFTGDEVAKYFLDKGVKVNELQIDPLLPSLRLQSTEDFTKWFKFFRDLGA 144 Query: 112 ---EEGVIQTVELIKRHPLLPPNVPIHGMIIDPATGAL 146 ++ ++ E++K HPL+P NV I + + T L Sbjct: 145 NSPDDIALKNAEILKNHPLIPKNVTISAYVYEVETHKL 182 >Rv1284 [P] COG0288 Carbonic anhydrase Length = 163 Score = 65.5 bits (158), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 42/148 (28%), Positives = 75/148 (50%), Gaps = 17/148 (11%) Query: 3 IITCMDTRLVELLPKAMNFKNGDVKIIKNAGAVISQPFGSVMRSVMVALYELDADEVIVV 62 I+ CMD RL + + + K G+ +I+NAG V++ V+RS+ ++ L E+I++ Sbjct: 32 IVACMDARLD--VYRMLGIKEGEAHVIRNAGCVVTD---DVIRSLAISQRLLGTREIILL 86 Query: 63 GHYECGMASLNADRMINSIKERGVSDEVLSTLENSGIKLTKWLRGFDNVEEGVIQTVELI 122 H +CGM + D +R + DE +GI+ T + + E V Q++ I Sbjct: 87 HHTDCGMLTFTDDDF-----KRAIQDE-------TGIRPTWSPESYPDAVEDVRQSLRRI 134 Query: 123 KRHPLLPPNVPIHGMIIDPATGALELVS 150 + +P + + + G + D ATG L V+ Sbjct: 135 EVNPFVTKHTSLRGFVFDVATGKLNEVT 162 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,353,184 Number of extensions: 433661 Number of successful extensions: 935 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 936 Number of HSP's successfully gapped: 6 Length of query: 156 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 59 Effective length of database: 27,191,033 Effective search space: 1604270947 Effective search space used: 1604270947 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)