BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0005.p (150 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sll1675 [E] COG0346 Lactoylglutathione lyase and related lyases 50 1e-06 >sll1675 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 150 Score = 50.4 bits (119), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 37/130 (28%), Positives = 63/130 (48%), Gaps = 18/130 (13%) Query: 34 LNWITLRVRDLEASLNFYHGILGL--------PIERRFESRGKQIVMLGTAEQPKVELIQ 85 ++ I L V+D+E S +FY ILGL P+ + ++ T + ++L Sbjct: 15 VHHIALNVKDMEKSCHFYGHILGLKPLAGEEIPLTLKTMVEEGKVSNFITPDGTVIDLFA 74 Query: 86 A---NDQAVKPECGVS----IGFEV--KSLDEAIEYLKSRGIPVLRGPVTPNPQLRFFYI 136 + AV P + + F++ + D+AI L++ G+ + GPVT P R Y Sbjct: 75 TPNLSPPAVDPHQSFTRTDHLAFDIAPEQFDQAIAVLEAHGVTIASGPVT-RPTGRGIYF 133 Query: 137 LDPDGFEVQL 146 DPDGF+V++ Sbjct: 134 YDPDGFQVEI 143 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.142 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,154,580 Number of extensions: 470134 Number of successful extensions: 1100 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 1114 Number of HSP's successfully gapped: 1 Length of query: 150 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 53 Effective length of database: 27,191,033 Effective search space: 1441124749 Effective search space used: 1441124749 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 111 (47.4 bits)