BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0004.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_638 [K] COG0583 Transcriptional regulator 64 3e-10 MA3213 [L] COG0675 Transposase and inactivated derivatives 60 4e-09 PA4797 [L] COG3547 Transposase and inactivated derivatives 60 4e-09 RSp0478 [L] COG3328 Transposase and inactivated derivatives 60 4e-09 SMb20778 [L] COG3335 Transposase and inactivated derivatives 60 4e-09 >aq_638 [K] COG0583 Transcriptional regulator Length = 303 Score = 63.5 bits (153), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 37/117 (31%), Positives = 64/117 (54%), Gaps = 5/117 (4%) Query: 1 VVDFEWYRSFCTIYKHNSVSEAAKARIMTQPAMSQHLASLEAEVGEALFIRTSRKIIPTE 60 ++D ++F T+ + S S+A++ +TQPA++Q + +LE VG LF R +++ TE Sbjct: 1 MIDISKLKTFVTVAELGSFSKASEVLYITQPAVTQQIKALEKTVGAKLFQRQGGRMVLTE 60 Query: 61 RGKQLYSQLAPLI----ESLEETTMNFKSASLPTLSSIKIGTPHEFYTEKVLPQLHK 113 GK++Y ++A L+ E L E K TL + TP E+ K++ + HK Sbjct: 61 EGKRIY-EIAKLLLNSYEGLMEEIARIKKDLKDTLFLVLSATPSEYIIPKIIAEFHK 116 >MA3213 [L] COG0675 Transposase and inactivated derivatives Length = 121 Score = 59.7 bits (143), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 8/115 (6%), Positives = 24/115 (20%), Gaps = 1/115 (0%) Query: 2 VDFEWYRSFCTIYKHNSVSEAAKARIMTQPAMSQHLASLEAEVGEALFIRTSRKIIPTER 61 + S + + A++ +L + R + + Sbjct: 1 MRLSQLLSDLNGIEIGFALCGTRRDRDINAAINIKSIALRYGNNSPYYGRIKPADLFSAG 60 Query: 62 GKQLYSQLAPLIESLEETTMNFKSASLPTLSSIKIG-TPHEFYTEKVLPQLHKCK 115 + + + T + S +S I + + Sbjct: 61 NVRSRKNNRIVGKLFHITKNIYNKLSPDPVSGNSIFRSSIIQDWGASSRAVRTHI 115 >PA4797 [L] COG3547 Transposase and inactivated derivatives Length = 338 Score = 59.7 bits (143), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 2/115 (1%), Positives = 20/115 (17%), Gaps = 1/115 (0%) Query: 2 VDFEWYRSFCTIYKHNSVSEAAKARIMTQPAMSQHLASLEAEVGEALFIRTSRKIIPTER 61 ++ ++ + + + + Sbjct: 1 MNLSRIGLDLAKQVFQVHGVDRHEHVVCRRQLKRAQVRDFFRQLPPCLVAMEACGSAHYW 60 Query: 62 GKQLYSQLAPLIESLEETTMNFKSASLPTLSSIKIG-TPHEFYTEKVLPQLHKCK 115 ++L + + + + + + +P + Sbjct: 61 ARELRELGHTVRLIAPQFVKPYVKGDKHDAHDAEAICEAASRPSMRYVPVKSAEQ 115 >RSp0478 [L] COG3328 Transposase and inactivated derivatives Length = 416 Score = 59.7 bits (143), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 2/115 (1%), Positives = 12/115 (10%), Gaps = 1/115 (0%) Query: 2 VDFEWYRSFCTIYKHNSVSEAAKARIMTQPAMSQHLASLEAEVGEALFIRTSRKIIPTER 61 + + + + + + Sbjct: 1 MPRKLKAKPVDLPAIPAELLEQFGNGPMTAEAINAATLALKKALIERALSGEMNHHLGYP 60 Query: 62 GKQLYSQLAPLIESLEETTMNFKSASLPTLSSIKIG-TPHEFYTEKVLPQLHKCK 115 + + + E + K Sbjct: 61 PGAAKPATVTNQRNGSGAKTVLTEDGPIRIEVPRDRDGSFEPLLIPKHERRFKGF 115 >SMb20778 [L] COG3335 Transposase and inactivated derivatives Length = 162 Score = 59.7 bits (143), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 3/115 (2%), Positives = 12/115 (10%), Gaps = 1/115 (0%) Query: 2 VDFEWYRSFCTIYKHNSVSEAAKARIMTQPAMSQHLASLEAEVGEALFIRTSRKIIPTER 61 + + Sbjct: 1 WTKTNMAPLRGWAPRGERLVGYAPFGHWNTMTFVAALRADRVSAPFILDGPINGERFRIY 60 Query: 62 GKQLYSQLAPLIESLEETTMNFKSASLPTLSSIKIG-TPHEFYTEKVLPQLHKCK 115 +Q+ + + + + K G + Sbjct: 61 VQQVLVPELKAGDIVILDNLGSHKGQEIRAAIRKAGARLFFLPKYSPDLNPIEKL 115 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,112,694 Number of extensions: 622981 Number of successful extensions: 2590 Number of sequences better than 1.0e-05: 1369 Number of HSP's gapped: 2595 Number of HSP's successfully gapped: 1369 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)