BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0001.p (369 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all2564 [G] COG0469 Pyruvate kinase 285 1e-76 STM1378 [G] COG0469 Pyruvate kinase 278 1e-74 VC0485 [G] COG0469 Pyruvate kinase 277 2e-74 ZpykF [G] COG0469 Pyruvate kinase 277 2e-74 pykF [G] COG0469 Pyruvate kinase 277 2e-74 >all2564 [G] COG0469 Pyruvate kinase Length = 476 Score = 285 bits (728), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 155/366 (42%), Positives = 225/366 (61%), Gaps = 14/366 (3%) Query: 5 LICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPVRIMG 64 +ICT+GPA+S+P L+ L+ GM ARLN SHG+++ H +PV IM Sbjct: 6 IICTVGPATSAPERLEALVEAGMNVARLNFSHGAYDFHAQTAQYLRQISADRQKPVAIMQ 65 Query: 65 DLQGPKIRLKSIQGDAVTLEEGQ--TFILDQSDEPGSRERAALDNPGVMEDILQGAAILI 122 DL GPKIRL ++ + + +E GQ TF+L + S + L P + + G ILI Sbjct: 66 DLCGPKIRLGTLPPEGLMVEAGQEVTFVLQEKG--SSLDELPLPLPTLFAMVRPGEPILI 123 Query: 123 NDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLEHH 182 NDG VKL VTD+ RI +GG++ +RKGVNLP T + + +ITEKD QDL+F ++ Sbjct: 124 NDGRVKLIVTDRDADRIRAIAKIGGLLSTRKGVNLPATRLPVSSITEKDLQDLRFGIDLS 183 Query: 183 VDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMIAR 242 VDW+A SF+R L+ + + GK + +I+KIE +AV +I++ +D IMIAR Sbjct: 184 VDWVAVSFVRSPYDLEPAQRMIEAAGKTIR--VIAKIERPEAVEQIDSIIDVADAIMIAR 241 Query: 243 GDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAVLD 302 GDLGVE+P +P IQK ++R C+ + VITATQML+SM+ P PTRAE TDV+ ++LD Sbjct: 242 GDLGVEMPIHEVPLIQKDIIRRCNQAGKPVITATQMLESMISAPDPTRAEATDVANSILD 301 Query: 303 GSDGVMLSAESSVGHYPVRSTQVLNTVAQFAENMREQGER--------GISLEELCADAP 354 G+D VMLS E++VG YPV + QV++ +A E ++G + G+S+ E A+A Sbjct: 302 GTDAVMLSGETAVGQYPVAAVQVMHDIAVTTEKSLQEGSKHCLSHEAGGLSVTESVAEAV 361 Query: 355 FDKAFK 360 A++ Sbjct: 362 CRIAYE 367 >STM1378 [G] COG0469 Pyruvate kinase Length = 470 Score = 278 bits (711), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 144/330 (43%), Positives = 209/330 (63%), Gaps = 5/330 (1%) Query: 5 LICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPVRIMG 64 ++CTIGP + S +L ++L GM RLN SHG + EHG TG+ I+ Sbjct: 6 IVCTIGPKTESEEMLSKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKKAAILL 65 Query: 65 DLQGPKIRLKSIQG-DAVTLEEGQTFILDQSDEP--GSRERAALDNPGVMEDILQGAAIL 121 D +GP+IR ++G + V+L+ GQTF +D+ G+ E A+ G D+ G +L Sbjct: 66 DTKGPEIRTIKLEGGNDVSLKAGQTFTF-TTDKSVVGNNEIVAVTYEGFTSDLSVGNTVL 124 Query: 122 INDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLEH 181 ++DG + +EVT ++ +V+ G +G KGVNLPG I LPA+ EKDKQDL F E Sbjct: 125 VDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEQ 184 Query: 182 HVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMIA 241 VD++A SFIR+ S + EIR+++ G + +ISKIE + + NF I+EASDGIM+A Sbjct: 185 GVDFVAASFIRKRSDVVEIREHLKAHGGENIQ-IISKIENQEGLNNFDEILEASDGIMVA 243 Query: 242 RGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAVL 301 RGDLGVE+P E + F QK ++ +C ++ VITATQML SM+++P PTRAE DV+ A+L Sbjct: 244 RGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIL 303 Query: 302 DGSDGVMLSAESSVGHYPVRSTQVLNTVAQ 331 DG+D VMLS ES+ G YP+ + ++ T+ + Sbjct: 304 DGTDAVMLSGESAKGKYPLEAVSIMATICE 333 >VC0485 [G] COG0469 Pyruvate kinase Length = 470 Score = 277 bits (709), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 145/332 (43%), Positives = 210/332 (63%), Gaps = 6/332 (1%) Query: 5 LICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPVRIMG 64 ++CTIGP + S L EL+ GM RLN SHG + EHG TG+ + I+ Sbjct: 6 IVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQLAILL 65 Query: 65 DLQGPKIR-LKSIQGDAVTLEEGQTFILDQSDEP-GSRERAALDNPGVMEDILQGAAILI 122 D +GP+IR +K GD V L GQ F + G++ER A+ G +D+ G IL+ Sbjct: 66 DTKGPEIRTIKLENGDDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVGNRILV 125 Query: 123 NDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLEHH 182 +DG +++EV + +V+ G +G KGVNLPG + LPA++EKDK DL+F E Sbjct: 126 DDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNLPGVSVNLPALSEKDKNDLKFGCEQG 185 Query: 183 VDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMIAR 242 VD++A SFIR+ S ++EIR+ ++ G + +ISKIE + + NF I+E SDGIM+AR Sbjct: 186 VDFVAASFIRKGSDVKEIREVLASHGGQNIQ-IISKIENQEGLDNFDEILELSDGIMVAR 244 Query: 243 GDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAVLD 302 GDLGVE+P E + F QK ++ +C+ ++ VITATQML SM+++P PTRAE DV+ A++D Sbjct: 245 GDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMD 304 Query: 303 GSDGVMLSAESSVGHYPVRSTQVLNTVAQFAE 334 G+D VMLS E++ G YPV + +++ AQ AE Sbjct: 305 GTDAVMLSGETAKGKYPVEAVKIM---AQIAE 333 >ZpykF [G] COG0469 Pyruvate kinase Length = 470 Score = 277 bits (709), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 144/330 (43%), Positives = 208/330 (63%), Gaps = 5/330 (1%) Query: 5 LICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPVRIMG 64 ++CTIGP + S +L ++L GM RLN SHG + EHG TG+ I+ Sbjct: 6 IVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILL 65 Query: 65 DLQGPKIRLKSIQG-DAVTLEEGQTFILDQSDEP--GSRERAALDNPGVMEDILQGAAIL 121 D +GP+IR ++G + V+L+ GQTF +D+ G+ E A+ G D+ G +L Sbjct: 66 DTKGPEIRTMKLEGGNDVSLKAGQTFTF-TTDKSVIGNSEMVAVTYEGFTTDLSVGNTVL 124 Query: 122 INDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLEH 181 ++DG + +EVT ++ +V+ G +G KGVNLPG I LPA+ EKDKQDL F E Sbjct: 125 VDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEQ 184 Query: 182 HVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMIA 241 VD++A SFIR+ S + EIR+++ G +ISKIE + + NF I+EASDGIM+A Sbjct: 185 GVDFVAASFIRKRSDVIEIREHLKAHGG-ENIHIISKIENQEGLNNFDEILEASDGIMVA 243 Query: 242 RGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAVL 301 RGDLGVE+P E + F QK ++ +C ++ VITATQML SM+++P PTRAE DV+ A+L Sbjct: 244 RGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIL 303 Query: 302 DGSDGVMLSAESSVGHYPVRSTQVLNTVAQ 331 DG+D VMLS ES+ G YP+ + ++ T+ + Sbjct: 304 DGTDAVMLSGESAKGKYPLEAVSIMATICE 333 >pykF [G] COG0469 Pyruvate kinase Length = 470 Score = 277 bits (709), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 144/330 (43%), Positives = 208/330 (63%), Gaps = 5/330 (1%) Query: 5 LICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPVRIMG 64 ++CTIGP + S +L ++L GM RLN SHG + EHG TG+ I+ Sbjct: 6 IVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILL 65 Query: 65 DLQGPKIRLKSIQG-DAVTLEEGQTFILDQSDEP--GSRERAALDNPGVMEDILQGAAIL 121 D +GP+IR ++G + V+L+ GQTF +D+ G+ E A+ G D+ G +L Sbjct: 66 DTKGPEIRTMKLEGGNDVSLKAGQTFTF-TTDKSVIGNSEMVAVTYEGFTTDLSVGNTVL 124 Query: 122 INDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLEH 181 ++DG + +EVT ++ +V+ G +G KGVNLPG I LPA+ EKDKQDL F E Sbjct: 125 VDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEQ 184 Query: 182 HVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMIA 241 VD++A SFIR+ S + EIR+++ G +ISKIE + + NF I+EASDGIM+A Sbjct: 185 GVDFVAASFIRKRSDVIEIREHLKAHGG-ENIHIISKIENQEGLNNFDEILEASDGIMVA 243 Query: 242 RGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAVL 301 RGDLGVE+P E + F QK ++ +C ++ VITATQML SM+++P PTRAE DV+ A+L Sbjct: 244 RGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIL 303 Query: 302 DGSDGVMLSAESSVGHYPVRSTQVLNTVAQ 331 DG+D VMLS ES+ G YP+ + ++ T+ + Sbjct: 304 DGTDAVMLSGESAKGKYPLEAVSIMATICE 333 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,601,516 Number of extensions: 928711 Number of successful extensions: 2326 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 2266 Number of HSP's successfully gapped: 29 Length of query: 369 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 261 Effective length of database: 25,922,359 Effective search space: 6765735699 Effective search space used: 6765735699 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)