BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0272.0002.p (315 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value XF1825 [MG] COG0451 Nucleoside-diphosphate-sugar epimerases 87 3e-17 FN1847 [MG] COG0451 Nucleoside-diphosphate-sugar epimerases 76 7e-14 all5345 [MG] COG0451 Nucleoside-diphosphate-sugar epimerases 70 5e-12 YGL001c [MG] COG0451 Nucleoside-diphosphate-sugar epimerases 69 8e-12 SPBC3F6.02c [MG] COG0451 Nucleoside-diphosphate-sugar epimerases 64 2e-10 >XF1825 [MG] COG0451 Nucleoside-diphosphate-sugar epimerases Length = 332 Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 81/266 (30%), Positives = 117/266 (43%), Gaps = 19/266 (7%) Query: 21 IDAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXXXXTFVYISAAG-IHMDNSGTL 79 +DAV H AA G + + NV GT VY S+ IH N Sbjct: 63 VDAVFHNAAKVGAWGSYTSYHQINVIGTQHVLDACRAENISKLVYTSSPSVIHRSNYPVE 122 Query: 80 IRDADESAPTFPNHFSAYLASKARADSLVLAANKPGFRTIALRPPAIWGPGDPFSRALPE 139 DAD+ P Y A+KA A+ VLAAN T+ALRP IWGPGDP +P Sbjct: 123 GLDADQ-VPYSNAVKVPYAATKAMAEQAVLAANSVDLTTVALRPRMIWGPGDP--HLMPR 179 Query: 140 AV---RSGQFAFIDHGDYPFSTCHVDNVVEAIQCALER-----GEGGHAFFIKDQEGQTF 191 V R+G+ ID G + ++DN +A A E G A+FI + E Sbjct: 180 LVARARAGRLRLIDDGRNLVDSTYIDNAAQAHFDAFEHLMPGAACAGKAYFISNGEPLQM 239 Query: 192 REFAASL---ANLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGDPPISRSMIRMIG 248 RE L AN ++ +S+S+ IG + +W++ G+P ++R ++ + Sbjct: 240 RELINKLLAAANAPPVT----QSLSFKTGYCIGAFCEMLWSLLPLLGEPLLTRFLVEQMS 295 Query: 249 REFTVNDAAARRELGYVGRTLRDAGL 274 + A+R+ GYV R + GL Sbjct: 296 TPHWYSIEPAKRDFGYVPRVSIEEGL 321 >FN1847 [MG] COG0451 Nucleoside-diphosphate-sugar epimerases Length = 328 Score = 76.3 bits (186), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 66/245 (26%), Positives = 109/245 (44%), Gaps = 11/245 (4%) Query: 23 AVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXXXXTFVYISAAGIHMDNSGTLIRD 82 AV+HAAAL G + F+ NV GT V++S+ I+ L Sbjct: 68 AVIHAAALSTVWGKWKDFYNVNVLGTKNVVQVCEEKNL-KLVFVSSPSIYAGAKDQLDVK 126 Query: 83 ADESAPTFPNHFSAYLASKARADSLVLAANKPGFRTIALRPPAIWGPGDP--FSRALPEA 140 DE AP N + Y+ SK A++++ ++ + +RP ++G GD R L Sbjct: 127 EDE-APK-ENDLNYYIKSKIMAENIIKSSK---LNYMIIRPRGLFGVGDTSIIPRLLELN 181 Query: 141 VRSGQFAFIDHGDYPFSTCHVDNVVEAIQCALERGEGGHAFF-IKDQEGQTFREFAASLA 199 + G F+D TC V+NV A++ ALE + + I + E F+E Sbjct: 182 KKIGIPLFVDGKQKVDITC-VENVAYALRLALENNQYSRKIYNITNDEPIEFKEILTLFF 240 Query: 200 NLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGDPPISRSMIRMIGREFTVNDAAAR 259 N G K +Y L+S + + + + R +PPI++ + ++ T+N A+ Sbjct: 241 NEMGTE-GKYLKWNYNLISPLVSFLEIFYKLFRIKKEPPITKYTLYLMRYSQTLNIDKAK 299 Query: 260 RELGY 264 +ELGY Sbjct: 300 KELGY 304 >all5345 [MG] COG0451 Nucleoside-diphosphate-sugar epimerases Length = 333 Score = 69.7 bits (169), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 52/187 (27%), Positives = 88/187 (47%), Gaps = 6/187 (3%) Query: 21 IDAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXXXXTFVYISAAGIHMDNSGTLI 80 +D V+H A L +GP + F NV G FV++S+ ++ N I Sbjct: 69 VDVVLHTAQLSEEAGPLKDFREINVAGAVNMAKAAKNAGVKCFVHLSSVMVYGFNYSDRI 128 Query: 81 RDADESAPTFPNHFSAYLASKARADSLVLAANKP-GFRTIALRPPAIWGPGD-PFSRALP 138 ES P ++ + Y +K A++ +L N P F I +R I+GPG P+ Sbjct: 129 T---ESGPLSSDN-NPYCQTKIEAETELLPLNSPPDFGLIIIRAGDIYGPGSIPWIVRPI 184 Query: 139 EAVRSGQFAFIDHGDYPFSTCHVDNVVEAIQCALERGEGGHAFFIKDQEGQTFREFAASL 198 +R FA+ + G + ++DN+++AI A+ + G F I D E +++E+ L Sbjct: 185 LMMRQKLFAYANEGQGVINHVYIDNLIDAIFLAVAKDAYGEIFNITDGEETSWKEYFMRL 244 Query: 199 ANLQGLS 205 A ++G S Sbjct: 245 AAMEGAS 251 >YGL001c [MG] COG0451 Nucleoside-diphosphate-sugar epimerases Length = 349 Score = 69.3 bits (168), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 73/266 (27%), Positives = 112/266 (42%), Gaps = 17/266 (6%) Query: 22 DAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXXXXTFVYISAAGIHMDNSGTLIR 81 + VVH A+ P + + NV GT VY S+AG+ + G + Sbjct: 79 NVVVHCASPMHGQNP-DIYDIVNVKGTRNVIDMCKKCGVNILVYTSSAGVIFN--GQDVH 135 Query: 82 DADESAPTFPNHFSAYLASKARADSLVLAANKPG--FRTIALRPPAIWGPGD-PFSRALP 138 +ADE+ P AY +KA A+ +VL AN P F T+ALRP I+GPGD L Sbjct: 136 NADETWPIPEVPMDAYNETKAIAEDMVLKANDPSSDFYTVALRPAGIFGPGDRQLVPGLR 195 Query: 139 EAVRSGQFAF-IDHGDYPFSTCHVDNVVEAIQCALER--------GEGGHAFFIKDQEGQ 189 + + GQ F I + F + NV +A A ++ G FFI + Sbjct: 196 QVAKLGQSKFQIGDNNNLFDWTYAGNVADAHVLAAQKLLDPKTRTAVSGETFFITNDTPT 255 Query: 190 TFREFAASLANLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGDPPISRSMIRMIGR 249 F A ++ G IDK + V AI + + W +P ++ ++++ Sbjct: 256 YFWALARTVWKADG-HIDKHVIVLKRPV-AICAGYLSEWVSKMLGKEPGLTPFRVKIVCA 313 Query: 250 EFTVNDAAARRELGYVGRTLRDAGLQ 275 N A A++ LGY R + G+ Sbjct: 314 YRYHNIAKAKKLLGYTPRVGIEEGIN 339 >SPBC3F6.02c [MG] COG0451 Nucleoside-diphosphate-sugar epimerases Length = 340 Score = 64.3 bits (155), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 56/185 (30%), Positives = 84/185 (45%), Gaps = 13/185 (7%) Query: 23 AVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXXXXTFVYISAAGIHMDNSGTLIRD 82 V+H A+ + R+ +F NVDGT VY S+AG+ + N LI + Sbjct: 73 VVIHTASPVH-NLARDIYFEVNVDGTANIIKACQKFNVDALVYTSSAGV-VFNGADLI-N 129 Query: 83 ADESAPTFPNHFSAYLASKARADSLVLAANKPGFRTIALRPPAIWGPGD-PFSRALPEAV 141 DES P H AY SKA A+ VL A+ +T ALR ++GPGD + + Sbjct: 130 VDESQPIPEVHMDAYNESKALAEKQVLEASSESLKTAALRVAGLFGPGDRQLVPGMLSVL 189 Query: 142 RSGQFAFIDHGD--YPFSTCHVDNVVEAIQCALER------GEGGHAFFIKDQEGQTFRE 193 ++GQ F GD F +++N A A++ G FFI + + F + Sbjct: 190 KNGQTKF-QLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNPTANGQVFFITNGQVIYFWD 248 Query: 194 FAASL 198 FA ++ Sbjct: 249 FARAI 253 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,913,982 Number of extensions: 762974 Number of successful extensions: 1791 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 1780 Number of HSP's successfully gapped: 18 Length of query: 315 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 209 Effective length of database: 26,153,027 Effective search space: 5465982643 Effective search space used: 5465982643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)