BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0023.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1423 [IQR] COG1028 Dehydrogenases with different specificitie... 364 e-101 BS_ydaD [IQR] COG1028 Dehydrogenases with different specificitie... 353 2e-97 CAC1576 [IQR] COG1028 Dehydrogenases with different specificitie... 332 5e-91 BH0800 [IQR] COG1028 Dehydrogenases with different specificities... 324 8e-89 SMa0187 [IQR] COG1028 Dehydrogenases with different specificitie... 288 9e-78 >CAC1423 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 299 Score = 364 bits (934), Expect = e-101, Method: Compositional matrix adjust. Identities = 172/272 (63%), Positives = 209/272 (76%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVIS 61 Q+RQPGIES M+PRP + Y ITGGDSGIGRA AV FAKE ADV I Sbjct: 26 QNRQPGIESVMRPRPIYNNPCYYASGKLRNKVALITGGDSGIGRAAAVAFAKEKADVAIV 85 Query: 62 YLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQH 121 YL E DA ETKR +E G++C+ + GD+ + FC++ + +T+ G L+IL+NNAA Q Sbjct: 86 YLYERNDAMETKRVIESLGQRCLTIEGDVRRDGFCREAVERTIRTFGNLNILVNNAAVQF 145 Query: 122 PQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYS 181 PQD I +IT+EQLE TFRTNIFSMFY+TKAA+P+LK GS+IINT SITAY G L+DYS Sbjct: 146 PQDSILNITNEQLENTFRTNIFSMFYITKAALPYLKYGSSIINTASITAYEGEKTLIDYS 205 Query: 182 STKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQ 241 STKGAI+SFTRSLS +L +KGIRVNAVAPGPIWTPLIP++F A +V+ FG PMKR GQ Sbjct: 206 STKGAIVSFTRSLSKSLVDKGIRVNAVAPGPIWTPLIPASFSAARVATFGKDTPMKRAGQ 265 Query: 242 PEELAPAYVYLASEDSSYVSGQVMHVNGGEIV 273 P ELAPAYVYLAS+DSSYV+G+V+H+NGG +V Sbjct: 266 PYELAPAYVYLASDDSSYVTGEVIHINGGRMV 297 >BS_ydaD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 286 Score = 353 bits (906), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 167/272 (61%), Positives = 206/272 (75%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVIS 61 Q RQPGIESEM P P +E Y ITGGDSGIGRAV+V +AKEGAD+ I Sbjct: 13 QSRQPGIESEMNPSPVYEYEDYKGADKLKGKVALITGGDSGIGRAVSVAYAKEGADIAIV 72 Query: 62 YLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQH 121 Y +EH DAEETK++VEQEG KC+L+AGD+G E FC + + KTV+ LG LDIL+NNA EQH Sbjct: 73 YKDEHEDAEETKKRVEQEGVKCLLIAGDVGEEEFCNEAVEKTVKELGGLDILVNNAGEQH 132 Query: 122 PQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYS 181 P++ I+DITSEQL RTF+TN +S FYLTK A+ +LK GS IINTTSI Y G+P L+DY+ Sbjct: 133 PKESIKDITSEQLHRTFKTNFYSQFYLTKKAIDYLKPGSAIINTTSINPYVGNPTLIDYT 192 Query: 182 STKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQ 241 +TKGAI +FTR+++ L + GIRVNAVAPGPIWTPLIP+TF + V++FG PM RPGQ Sbjct: 193 ATKGAINAFTRTMAQALVKDGIRVNAVAPGPIWTPLIPATFPEETVAQFGQDTPMGRPGQ 252 Query: 242 PEELAPAYVYLASEDSSYVSGQVMHVNGGEIV 273 P E YV LAS++SSY++GQ +HVNGG V Sbjct: 253 PVEHVGCYVLLASDESSYMTGQTLHVNGGNFV 284 >CAC1576 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 285 Score = 332 bits (850), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 158/273 (57%), Positives = 201/273 (73%), Gaps = 1/273 (0%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXX-XXXXITGGDSGIGRAVAVHFAKEGADVVI 60 Q+ QPG E EM P P + Y ITGGDSGIGRAV+V +A EGADVVI Sbjct: 12 QNHQPGYEYEMNPAPIYYDEKYNKSRGLLKGKTAIITGGDSGIGRAVSVAYAHEGADVVI 71 Query: 61 SYLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQ 120 Y +EH DA+ETK+ ++ GRKC L+ GDIG FC I T+ K+DIL+NNAA Q Sbjct: 72 IYYDEHQDADETKKIIDGLGRKCTLMPGDIGDTNFCNYAIENTIRAYDKIDILVNNAAMQ 131 Query: 121 HPQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDY 180 +PQ+ I DI++EQ +RTF+TN++ FY+T+AA+ +K GS+IINTTS+ A++G+ L+DY Sbjct: 132 YPQNSIADISNEQFDRTFKTNVYGTFYMTRAAISKMKAGSSIINTTSVVAFKGNETLIDY 191 Query: 181 SSTKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPG 240 S TKGAIL+FT+SLS +L + GIRVNAVAPGPIWTPLI ++FD KVSEFG P++RPG Sbjct: 192 SMTKGAILAFTKSLSTSLVKSGIRVNAVAPGPIWTPLISASFDENKVSEFGSNVPLERPG 251 Query: 241 QPEELAPAYVYLASEDSSYVSGQVMHVNGGEIV 273 QP E A AYV+LASE SSY++GQ +HVNGGEIV Sbjct: 252 QPVECAGAYVFLASEMSSYITGQTIHVNGGEIV 284 >BH0800 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 299 Score = 324 bits (831), Expect = 8e-89, Method: Compositional matrix adjust. Identities = 154/271 (56%), Positives = 200/271 (73%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVIS 61 Q PG+E M+PRP E ITGGDSGIGRAVA FAKEGAD+VIS Sbjct: 26 QPWHPGLEYLMEPRPISENPHRPHSHKLLDRVAIITGGDSGIGRAVAYAFAKEGADLVIS 85 Query: 62 YLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQH 121 YL+EH DA ETK++VE+ GR C+LV GD+ E ++ KT+E GKLD+L+NN A Q Sbjct: 86 YLSEHEDAIETKKRVEEIGRDCLLVPGDLQHEQMSVKVVEKTIERFGKLDVLVNNHAVQF 145 Query: 122 PQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYS 181 Q + DIT EQL+ TFRTNI++ F++TKAA+PHLKKGS IINTTS+T+Y G QL+DY+ Sbjct: 146 RQTSLLDITEEQLDITFRTNIYAYFFMTKAALPHLKKGSAIINTTSVTSYLGHSQLIDYA 205 Query: 182 STKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQ 241 +TKGA+ +FTR+LS +LA++ IRVN VAPGPIWTPLIPS++ A + FG P+ RPGQ Sbjct: 206 ATKGAVTTFTRALSGSLADQEIRVNGVAPGPIWTPLIPSSYPAHSIPTFGSDTPLSRPGQ 265 Query: 242 PEELAPAYVYLASEDSSYVSGQVMHVNGGEI 272 P E+AP++V+LAS+DS Y++GQ++H NGG + Sbjct: 266 PFEVAPSFVFLASDDSKYLTGQILHPNGGRV 296 >SMa0187 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 256 Score = 288 bits (736), Expect = 9e-78, Method: Compositional matrix adjust. Identities = 139/236 (58%), Positives = 179/236 (75%), Gaps = 1/236 (0%) Query: 36 ITGGDSGIGRAVAVHFAKEGADVVISYLNEHADAEETKRQVEQEGRKCILVAGDIGVEAF 95 ITGGDSGIGR+VAV FA+EGADV I +L+E DA++TK VE+EGRKC+++ GD+ +F Sbjct: 20 ITGGDSGIGRSVAVLFAREGADVAIVHLDESQDADDTKAAVEKEGRKCLVIKGDVKDASF 79 Query: 96 CQDLINKTVEGLGKLDILINNAAEQHPQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPH 155 C+ + KTV LG+LDILINNAA Q IED+T E + T +TN++ FY+ KAA+PH Sbjct: 80 CRKAVEKTVMQLGRLDILINNAAFQVHTRDIEDLTDEHFDETLKTNLYGYFYMAKAAIPH 139 Query: 156 LKKGSTIINTTSITAYRGSPQLLDYSSTKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWT 215 LK GS IINT S+T GS +LLDYS TKG I +FTR+LS +L KGIRVNAVAPGP+WT Sbjct: 140 LKNGSAIINTGSVTGLTGSKELLDYSMTKGGIHAFTRALSGHLVPKGIRVNAVAPGPVWT 199 Query: 216 PLIPSTFDAKKVSEFGGTQPMKRPGQPEELAPAYVYLAS-EDSSYVSGQVMHVNGG 270 PL PS +A+ V +FG PMKR QPEE+APAYV+LAS + SSY++G+++ + GG Sbjct: 200 PLNPSDKEAEDVEKFGSQTPMKRAAQPEEIAPAYVFLASPQMSSYITGEILPIVGG 255 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,235,606 Number of extensions: 769690 Number of successful extensions: 2185 Number of sequences better than 1.0e-05: 340 Number of HSP's gapped: 1589 Number of HSP's successfully gapped: 340 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)