BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0271.0022.p
         (200 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BS_tenI [H] COG0352 Thiamine monophosphate synthase                    89   3e-18
CAC2920 [H] COG0352 Thiamine monophosphate synthase                    59   3e-09
aq_558 [H] COG0352 Thiamine monophosphate synthase                     53   4e-07
FN1752 [H] COG0352 Thiamine monophosphate synthase                     52   8e-07
BS_thiC [H] COG0352 Thiamine monophosphate synthase                    51   9e-07

>BS_tenI [H] COG0352 Thiamine monophosphate synthase
          Length = 205

 Score = 89.4 bits (220), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 55/185 (29%), Positives = 82/185 (44%)

Query: 3   EIHLISDGKLEPGRFINLAAAVYPLVDYIHLREKHRPALELLDMAKQLLLAGVPASSLVI 62
           E+H I+D          +   +   VD+IH+RE+ + A ++L +   +   G+    LV+
Sbjct: 2   ELHAITDDSKPVEELARIIITIQNEVDFIHIRERSKSAADILKLLDLIFEGGIDKRKLVM 61

Query: 63  NDRLDXXXXXXXXXXXXXWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYGLFG 122
           N R+D                                                 DY LFG
Sbjct: 62  NGRVDIALFSTIHRVQLPSGSFSPKQIRARFPHLHIGRSVHSLEEAVQAEKEDADYVLFG 121

Query: 123 HVFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVMSGICS 182
           HVF T CK G + RG+ LL++  +R  +P+IAIGG+ P     V + GA GIAVMSGI S
Sbjct: 122 HVFETDCKKGLEGRGVSLLSDIKQRISIPVIAIGGMTPDRLRDVKQAGADGIAVMSGIFS 181

Query: 183 TEDPV 187
           + +P+
Sbjct: 182 SAEPL 186


>CAC2920 [H] COG0352 Thiamine monophosphate synthase
          Length = 195

 Score = 59.3 bits (142), Expect = 3e-09,   Method: Compositional matrix adjust.
 Identities = 29/62 (46%), Positives = 38/62 (61%)

Query: 117 DYGLFGHVFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAV 176
           DY L G+++ TACKPG + RGL  +    +   +P IAIGGI   N  ++I  GA G AV
Sbjct: 115 DYVLAGNIYETACKPGLKGRGLEFVNSISKNITIPEIAIGGISEENVQELINSGANGAAV 174

Query: 177 MS 178
           MS
Sbjct: 175 MS 176


>aq_558 [H] COG0352 Thiamine monophosphate synthase
          Length = 215

 Score = 52.8 bits (125), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 28/69 (40%), Positives = 39/69 (56%)

Query: 117 DYGLFGHVFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAV 176
           DY  FG ++ T+ K   +  G+  L EAV+ S+ P++ IGGI P    +V+R G   IAV
Sbjct: 122 DYIGFGSIYHTSTKENYKLVGVEALKEAVKISQKPIVCIGGIMPYRVPEVVRAGCRNIAV 181

Query: 177 MSGICSTED 185
            SGI    D
Sbjct: 182 SSGILGFAD 190


>FN1752 [H] COG0352 Thiamine monophosphate synthase
          Length = 206

 Score = 51.6 bits (122), Expect = 8e-07,   Method: Compositional matrix adjust.
 Identities = 23/63 (36%), Positives = 34/63 (53%)

Query: 118 YGLFGHVFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVM 177
           Y + GH+F T CK   + RGL  + E      +P+  IGG+   N+  VI  GA G+ +M
Sbjct: 141 YIVAGHIFETDCKKDLEPRGLKFIQELSSTLTIPIFVIGGMNQENSHLVINSGAFGVCMM 200

Query: 178 SGI 180
           S +
Sbjct: 201 SSL 203


>BS_thiC [H] COG0352 Thiamine monophosphate synthase
          Length = 631

 Score = 51.2 bits (121), Expect = 9e-07,   Method: Compositional matrix adjust.
 Identities = 6/73 (8%), Positives = 13/73 (17%), Gaps = 4/73 (5%)

Query: 117 DYGLFGHVFPTACKPGQQE-RGLGLLAEAVRRS--RMPLIAIGGIEPGNAAQVIRQGAAG 173
              L         +  Q      G++             +    I            + G
Sbjct: 133 SGVLTPKRAKAGRRVTQLHYARQGII-TPEMEFIAIRENMGRERIRSEVLRHQHPGMSFG 191

Query: 174 IAVMSGICSTEDP 186
             +   I +    
Sbjct: 192 AHLPENITAEFVR 204


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.322    0.141    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 9,951,320
Number of extensions: 347893
Number of successful extensions: 704
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 707
Number of HSP's successfully gapped: 5
Length of query: 200
Length of database: 38,378,431
Length adjustment: 101
Effective length of query: 99
Effective length of database: 26,729,697
Effective search space: 2646240003
Effective search space used: 2646240003
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 113 (48.1 bits)