BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0014.p (814 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy1302 [G] COG0366 Glycosidases 172 2e-42 alr0169 [G] COG0366 Glycosidases 162 3e-39 CC2286 [G] COG0366 Glycosidases 147 6e-35 SPAC27E2.01 [G] COG0366 Glycosidases 134 7e-31 DR1472 [G] COG0366 Glycosidases 134 8e-31 >SPy1302 [G] COG0366 Glycosidases Length = 711 Score = 172 bits (437), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 178/607 (29%), Positives = 248/607 (40%), Gaps = 116/607 (19%) Query: 6 PTQVEAASIGTVTEN-----DTIYQIMVDRFNDGDTSNNATGAAIRYGENSEEDFRYMKG 60 P V AA +VT DTIYQI+ DRFNDG+TSNN G + +N D + G Sbjct: 25 PLTVSAADNASVTNKADFSTDTIYQIVTDRFNDGNTSNN--GKTDVFDKN---DLKKYHG 79 Query: 61 GDWQGVIDKLP--YIHNMGYTAIWISPVAEPQVTSRDNNGTGKNTAYHGYNVKNPNAANP 118 GDWQG+I K+ Y+ +MG +AIWIS E + +NG+ AYHGY K+ N Sbjct: 80 GDWQGIIAKIKDGYLTDMGISAIWISSPVENIDSIDPSNGSA---AYHGYWAKDFFKTNQ 136 Query: 119 YFGTKEKLKELVDSAHALGIKVIIDVVPNHIGDYMLGSQAYYDIPGLQPAAPFNNPAWYH 178 +FGT+ ++LV AH IKV+ID PNH + F + Sbjct: 137 HFGTEADFQQLVKVAHQHHIKVVIDFAPNHTS------------TAEKEGTTFKEDGALY 184 Query: 179 HNGDINWSLADGR-----YDQWAQ-DYMEN---HXXXXXXXXXXXVPAAKQAIFNSIKGW 229 NG + +D + ++ W EN H P Q + +I W Sbjct: 185 KNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYHSMYGLADLNNINPKVDQYMKEAIDKW 244 Query: 230 FDYTGADGARVDAAKLIKP-------TDIGELQNLLGVNTFGENFDGNAE--FVSRWVGP 280 D G DG RVDA K + + I E N+ FGE F G+ + + Sbjct: 245 LDL-GVDGIRVDAVKHMSQGWQKNWLSHIYEKHNVF---VFGEWFSGHTDDDYDMTTFAN 300 Query: 281 NKEWGMLDFPLFFSVLNSFAYGQSFD-SNIKSTLAQDSYYNGNANHMVTFIDNHDRNRFL 339 N G+LDF ++ + +F + L VTFIDNHD RF Sbjct: 301 NSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVLENRDQVTNEVTDQVTFIDNHDMERFA 360 Query: 340 TEAGGNVDKMQNALSFIFTVRGTPVVFQGTEQNKGNGNGQIMTGGIADTWNRWSMVKRDA 399 T+ N + A + + T RG P ++ GTEQ G D NR M Sbjct: 361 TKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ---------YATGDKDPNNRGDMPS--- 408 Query: 400 GGNVLENYFNENTSTYKHIAKLNDIRKNNPALRTGTQREMWSAQNLYAFSRRIDSGTNVG 459 FN+ + YK I+KL +RK N AL GT + W + ++ F R+ G +V Sbjct: 409 --------FNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFERKF--GNHVA 458 Query: 460 QEVIS-------VFSNAS----------------GGSQTVTIPLRAESS--LTAGTVLIN 494 I+ +NA GG + + SS L AG V + Sbjct: 459 LVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSFELGAGQVAVW 518 Query: 495 QLNTSDKVTVQAG------GTTGKQIT------------VTVGANSAKIYSKTQPVVDTE 536 DK T Q G G G +IT VT G SA+I S + ++ + Sbjct: 519 TYEGEDK-TPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEILSWSDTLITLK 577 Query: 537 APSVPSN 543 P+VP+N Sbjct: 578 VPTVPAN 584 >alr0169 [G] COG0366 Glycosidases Length = 359 Score = 162 bits (409), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 0/574 (0%), Positives = 0/574 (0%), Gaps = 77/574 (13%) Query: 5 PPTQVEAASIGTVT---ENDTIYQIMVDRFNDGDTSNNATGAAIRYGENSEEDFRYMKGG 61 T D + + E + Sbjct: 32 VVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLP-TI 90 Query: 62 DWQGVIDKLPYIHNMGYTAIWISPVAEPQVTSRDNNGTGKNTAYHGYNVKNPNAANP-YF 120 Q + + V + G + A Sbjct: 91 SAQHFHTAVHNEEQLAALEEASLDEPV-TVWMK----LDTGMHRLGVRPEQAEAFYHRLT 145 Query: 121 GTKEK-------------LKELVDSAHALGIKVIIDVVPNHIGDYMLGSQAYYDIPGLQP 167 K + + + S P Sbjct: 146 QCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSH- 204 Query: 168 AAPFNNPA--WYHHNGDINWSLADGRYDQWAQDY-MENHXXXXXXXXXXXVPAAKQAIFN 224 + + D+ + A + + Sbjct: 205 FDWVRPGIILYGVSPLED---------RSTGADFGCQPVMSLTSSLIAVREHKAGEPVGY 255 Query: 225 SIKGWFDYTGADGARVDAAKLIKPTDIGELQNLLG--------VNTFGE-NFDGNAEFVS 275 + V V G D + Sbjct: 256 GGTWVSE-RDTRLGVVAM---GYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLG 311 Query: 276 RWVGPNKEWGMLDFPLFFSVLNSFAYGQSFDSN-IKSTLAQDSYYNGNANHMVTFIDNHD 334 ++ + V + I ++ + Sbjct: 312 PQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYV- 370 Query: 335 RNRFLTEAGGNVDKMQNALSFIFTVRGTPVVFQGTEQNKGNGNGQIMTGGIADTWNRWSM 394 Sbjct: 371 ------ 424 Query: 395 VKRDAGGNVLENYFNENTSTYKHIAKLNDIRKNNPALRTGTQREMWSAQNLYAFSRRIDS 454 Sbjct: 425 ----------- 473 Query: 455 GT-----NVGQEVI--SVFSNASGGSQTVTIPLRAESSLTAGTVLINQLNTSDKVTV-QA 506 Sbjct: 474 - 532 Query: 507 GGTTGKQITVTVGANSAKIYSKTQPVVDTEAPSV 540 Sbjct: 533 566 >CC2286 [G] COG0366 Glycosidases Length = 580 Score = 147 bits (372), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 147/581 (25%), Positives = 240/581 (41%), Gaps = 115/581 (19%) Query: 19 ENDTIYQIMVDRFNDGDTSNNATGAAIRYGENSEEDFR--YMKGGDWQGVIDKLPYIHNM 76 +++ IY ++ DRF +GD +N+ G A+ + + R + GGD +GV +L YI + Sbjct: 18 QDEVIYFVLPDRFENGDATNDHGGPALGALADGFDPARAGFYHGGDLKGVTRRLDYIQGL 77 Query: 77 GYTAIWISPVAEPQVTSRDNNGTG--KNTAYHGYNVKNPNAANPYFGTKEKLKELVDSAH 134 G TA+W++P+ +R G ++ A+HGY + + +P+ GTK K LVD+AH Sbjct: 78 GATAVWLAPI----FVNRAVQGPPGQESAAHHGYWITDFTDVDPHLGTKADFKALVDAAH 133 Query: 135 ALGIKVIIDVVPNHIGDYMLGSQ------AY----------------------------- 159 A G+KV +D+V NH D + + AY Sbjct: 134 ARGMKVYMDIVLNHTADVIQYKECPDNRCAYRGVSDYPYVRRGGVDGAPINDGFDGKDFS 193 Query: 160 -----------YDIPGLQPA---APFNNPAWYHHNGDINWSLADGRYDQWA--QDYMENH 203 Y PG + A A N+P +YH+ GD ++ +A H Sbjct: 194 KLKRPDWAYTPYVPPGQERAKTPAWLNDPIYYHNRGDSTFAGESSLLGDFATLDGVFTEH 253 Query: 204 XXXXXXXXXXXVPAAKQAIFNSIKGWFDYTGADGARVDAAKLIKP----------TDIGE 253 P + W D G DG R+D A+ + P + Sbjct: 254 ------------PRVVEGFIEVYGRWIDDFGIDGYRIDTARHVNPEFWQAFVPAMLARAK 301 Query: 254 LQNLLGVNTFGENFDGNAEFVSRWVGPNKEWGMLDFPLFFSVLNSFAYGQSFDSNIKSTL 313 + + + FGE FD + ++R V +K +LDF F + + A G++ + S Sbjct: 302 AKGIENFHIFGEVFDADVATLARHVKVDKLPTVLDF-AFQAAVTDVALGRAGPDRLASVF 360 Query: 314 AQDSYYNG---NANHMVTFIDNHDRNR---FLTEAGGNVD------KMQNALSFIFTVRG 361 A D Y G A + TF+ NHD R F+ +A + ++ A + + T RG Sbjct: 361 AGDVVYEGGEATARQLPTFLGNHDMGRIGHFVLKAHPKIGDDALLARITLANAIMLTARG 420 Query: 362 TPVVFQGTEQN-KGNGN----GQIMTGGIADTWNRWSMVKRDAGGNVLENYFNENTSTYK 416 P ++ G EQ G+G+ + M ++N +V A G F ++ Y+ Sbjct: 421 VPTIYYGDEQGFTGDGDYADSREDMFPSQVASYNDNRLVGSSAKGP--RTAFQTDSVLYR 478 Query: 417 HIAKLNDIRKNNPALRTGTQ--REMWSAQNLYAFSRRIDSGTNVGQEVISVFSNASGGSQ 474 IA L +R PALR G Q R L AFSR + G EV++VF+ Sbjct: 479 RIAALAKLRGMTPALREGRQVTRIAEGKPGLLAFSRLM-----AGTEVLAVFN------- 526 Query: 475 TVTIPLRAESSLTAGTVLINQLNTSDKVTVQAGGTTGKQIT 515 T P++A + G+ L+ + + A G+ ++ Sbjct: 527 TSDKPIKARVPVEMGSAAWKSLHGACISSAVAPGSYAVELA 567 >SPAC27E2.01 [G] COG0366 Glycosidases Length = 491 Score = 134 bits (336), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 133/492 (27%), Positives = 207/492 (42%), Gaps = 96/492 (19%) Query: 22 TIYQIMVDRF--NDGDTSNNATGAAIRYGENSEEDFRYMKGGDWQGVIDKLPYIHNMGYT 79 IYQI+ DRF +D T+ +TG R GG W+G+I KL YI ++G T Sbjct: 28 CIYQILTDRFALDDHCTTAPSTG-------------RMYLGGTWRGIIQKLDYIQSLGCT 74 Query: 80 AIWISPVAEPQVTSRDNNGTGKNTAYHGYNVKNPNAANPYFGTKEKLKELVDSAHALGIK 139 A+WISP+ + TG AYHGY ++ NP+FGTK+ L ELVD H + Sbjct: 75 AVWISPIVK-----NIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQDLTELVDQLHKRNML 129 Query: 140 VIIDVVPNHI---GDYMLGSQAYYDIPGLQPAAPFNNPAWYH-----HNGDINWSLADGR 191 +ID+V NH+ GD + Y APFN+P+ YH HN D W Sbjct: 130 CMIDIVVNHMAHAGDSPIDYSKY---------APFNSPSHYHPKRFLHNYDDTWDCEIA- 179 Query: 192 YDQWAQDYMENHXXXXXXXXXXXVPAAKQAIFNSIKGW----FDYTGADGARVDAAKLIK 247 W D + Q + N + W DG R+D AK ++ Sbjct: 180 ---WLGD---------EVVSLMDIRTEDQEVHNFFQNWIRDLIQTYHFDGLRIDTAKHVQ 227 Query: 248 PTDIGELQNLLGVNTFGENFDGNAEFVSRWVG--PNKEWGMLDFPLFFSVLNS-FAYGQS 304 V FGE + G+ +F+++++ P+ ++PL++ + N+ F QS Sbjct: 228 KEFYPPFIAAANVFAFGEVYHGDPKFIAKYLEYIPSAA----NYPLYYQIENTFFPPKQS 283 Query: 305 FDSNIKSTLAQDSYYNGNANHMVTFIDNHDRNRFLTEAGGNVDKMQNALSFIFTVRGTPV 364 + + + + + + + F +NHD RFL + + + N L+ + G P+ Sbjct: 284 MNIFYQKAILEARATSMDTTILGNFTENHDVPRFLNRS-TDYSLLCNTLTLLLFTDGIPI 342 Query: 365 VFQGTEQNKGNGNGQIMTGGIADTWNRWSMVKRDAGGNVLENYFNENTSTYKHIAKLNDI 424 +FQG EQ M G D NR ++ + +N+ ++ + KL + Sbjct: 343 IFQGQEQ---------MYAGGHDPENRDALWTSN---------YNQQNPIFQFLKKLIKL 384 Query: 425 RK----NNPALRTGTQREMWSAQNLYAFSRRIDSGTNVGQEVISVFSNASGGSQTVTIPL 480 R+ N T ++ +++Y F R VI V SNA S T Sbjct: 385 RQFLVDNVSGFTTELSNMLFVNEHVYVFRR---------PGVIIVVSNAGSNSDVDT--- 432 Query: 481 RAESSLTAGTVL 492 AE S+T L Sbjct: 433 SAEFSITERESL 444 >DR1472 [G] COG0366 Glycosidases Length = 483 Score = 134 bits (336), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 126/441 (28%), Positives = 179/441 (40%), Gaps = 85/441 (19%) Query: 13 SIGTVTENDTIYQIMVDRFNDGDTSNNATGAAIRYGENSEEDFRYMKGGDWQGVIDKLPY 72 S G E IYQ+M DRF DGD SNNA + R GGD G+ KLPY Sbjct: 23 STGPSFEGQIIYQVMPDRFFDGDPSNNAG--------VDRANLRAWHGGDLAGLTQKLPY 74 Query: 73 IHNMGYTAIWISPVAEPQVTSRDNNGTGKNTAYHGYNVKNPNAANPYFGTKEKLKELVDS 132 I +G TAIW++P+ Q + AYHGY + +P+FG+ + + Sbjct: 75 IQKLGATAIWMTPIYRQQTAK-----SFDTAAYHGYWPADFRQVDPHFGSMATFDTFMKA 129 Query: 133 AHALGIKVIIDVVPNHIGDYMLGSQAYYDIPGLQPAAPFNNPAWYH--------HNGDIN 184 A G+KV++D V NH G Y+ P ++ NP W++ N D++ Sbjct: 130 AKGAGMKVVLDQVINHYG---------YEAPAVK-----ANPGWFNGKAQCDATKNKDVD 175 Query: 185 WSLADGRYDQWAQDYMENHXXXXXXXXXXXVPAAKQAIFNSIKGWFDYTGADGARVDAAK 244 LA G D VPA +Q +F + W G DG R DA K Sbjct: 176 CPLA-GLPD-----------------LRQSVPAVRQQLFGNGDFW-RARGVDGFRYDAIK 216 Query: 245 LI-KP--TDIGELQNLLGVNTFGENFDGNAEFVSRWVGPNKEWGMLDFPLFF--SVLNSF 299 + +P D+ + G T GE +D +A V+ W ++ G LF + + Sbjct: 217 HVERPFLLDLLKKDRAAGTWTLGEWYDADAGTVAEW----QKQGFDSLFLFSLQAAMRGS 272 Query: 300 AYGQSFDSNIKSTLAQDSYYNGNANHMVTFIDNHDRNRF----LTEAGGNVDKMQNALSF 355 G S N++ L + + F+DNHD RF E G + + L Sbjct: 273 VMGASSLVNVREVLRRSDEL-ARPGEVALFLDNHDVPRFSQGSFFEDEGQA-RTKYGLRA 330 Query: 356 IFTVRGTPVVFQGTEQNKGNGNGQIMTGGIADTWNRWSMVKRDAGGNVLENYFNENTSTY 415 + T+RG PV++QGTE I G AD NR M + + Y Sbjct: 331 LLTLRGVPVLYQGTE---------IAMRGGADPDNRRDM-------RFESQWTPAEKAVY 374 Query: 416 KHIAKLNDIRKNNPALRTGTQ 436 +RK +P L GTQ Sbjct: 375 NVARDAIAVRKASPPLSRGTQ 395 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 59,933,215 Number of extensions: 2500773 Number of successful extensions: 3665 Number of sequences better than 1.0e-05: 98 Number of HSP's gapped: 3552 Number of HSP's successfully gapped: 101 Length of query: 814 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 699 Effective length of database: 25,115,021 Effective search space: 17555399679 Effective search space used: 17555399679 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 120 (50.8 bits)