BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0011.p (585 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2643 [T] COG0642 Signal transduction histidine kinase 345 1e-94 SA1515 [T] COG0642 Signal transduction histidine kinase 216 1e-55 lin2061 [T] COG0642 Signal transduction histidine kinase 213 5e-55 BS_phoR_3 [T] COG0642 Signal transduction histidine kinase 200 5e-51 BS_resE_4 [T] COG0642 Signal transduction histidine kinase 188 2e-47 >lin2643 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 345 bits (885), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 0/585 (0%), Positives = 0/585 (0%), Gaps = 20/585 (3%) Query: 3 LIGISMIGAGITMAKLFKDSHISALEENMSREINLLSGTFQFTDTDSPGAVSYYTEQAEH 62 G A T + S L + + + Q Sbjct: 15 PTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNSVCGHPQLGE 74 Query: 63 IAKLTNSRITFITKDGKVIGDSEKNPLEMDNHSTREE--EVLAAKEGIGRAIRYSDTLNR 120 + R ++ P + E R + Sbjct: 75 SNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQINDD 134 Query: 121 EMLYVAGAVVSDQGFDGYIRLSMALDAVTEGLNRAW--MVMAGGLVLLFIAATFVSYKVA 178 E++ ++D + Sbjct: 135 EVMDYQWCDLADVLHGIDATPWAFSPWMVMQATNREAR-- 192 Query: 179 SGMTSPLEQITRVARRITDLDYDARVPMNRRDEIGQLATAINAMADSLQAQLKTIRDNED 238 Sbjct: 193 252 Query: 239 LLQSVLDNMTGGIVMINAEGEIALLNRAAEQLLDVKNSEMAGHSFKELKHHYELSRLIEE 298 Sbjct: 253 -- 310 Query: 299 GVSRNEAVHEERSIYNPTERIVRLDGVPMI-QDGSNRGMLFLLQEVTEIRRLEKMRSEFV 357 Sbjct: 311 370 Query: 358 ANVSHELKTPVAAVKGFAETLLGGGVKDEKTARSFLQIIYDENERLNRLIGDILELSKIE 417 Sbjct: 371 430 Query: 418 SKRAQLECSPVHLIEFFDSLLETLSKVAEKKNISLNASVPEE----LFMEGDEDKLRQIF 473 Sbjct: 431 -- 488 Query: 474 INLISNAINYTQDGGSXXXXXXXGQKADGTETVVFTVRDTGMGIPRKDLPRIFERFYRVD 533 Sbjct: 489 ----- 543 Query: 534 KAXXXXXXXXXXXXXIVKHLVELHHGSISVESDLGIGSSFILELP 578 Sbjct: 544 588 >SA1515 [T] COG0642 Signal transduction histidine kinase Length = 554 Score = 216 bits (549), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 135/434 (31%), Positives = 231/434 (53%), Gaps = 20/434 (4%) Query: 148 VTEGLNRAWMVMAGGLVLLFIAATFVSYKVASGMTSPLEQITRVARRITDLDYDARVPMN 207 +TE W + +++ I F S + P++++T + + + Y RVP + Sbjct: 124 ITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPES 183 Query: 208 RRDEIGQLATAINAMADSLQAQLKTIRDNEDLLQSVLDNMTGGIVMINAEGEIALLNRAA 267 E L + N +A LQ + + L++ ++N+ I+MI+ G+I + N+A Sbjct: 184 NVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAY 243 Query: 268 EQLLDVK-NSEMAGHSFKELKHHY---ELSRLIEEGVSRNEAVHE--ERSIYNPTERIVR 321 + ++ N E G+ H Y E+ +LI E + ++E E +I + Sbjct: 244 YEQFNISHNIEQVGY------HGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFD 297 Query: 322 LDGVPMI--QDGSNRGMLFLLQEVTEIRRLEKMRSEFVANVSHELKTPVAAVKGFAETLL 379 + VP++ S +G+L ++ ++T +++LE +R EFVANVSHELKTP+ ++KGFAETL+ Sbjct: 298 ISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLI 357 Query: 380 GGGVKDEKTARSFLQIIYDENERLNRLIGDILELSKIESKRAQLECSPVHLIEFFDSLLE 439 G D ++ FL II E+ R+ L+ D+L+LS IE + +L+ ++L + +++ Sbjct: 358 DGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIE-QHTELDTDYMNLSDLTRRIID 416 Query: 440 TLSKVAEKKNISLNASVPEELFMEGDEDKLRQIFINLISNAINYTQDGGSXXXXXXXGQK 499 + A +KNIS++ + +++ ++ E K+ Q+ NL++NAINY+ + G Sbjct: 417 NMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDF 476 Query: 500 ADGTETVVFTVRDTGMGIPRKDLPRIFERFYRVDKAXXXXXXXXXXXXXIVKHLVELHHG 559 V+F V+D G+GI +D RIFERFYRVDKA I KH+VE H G Sbjct: 477 R-----VIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQG 531 Query: 560 SISVESDLGIGSSF 573 +I V S +G GS+F Sbjct: 532 NIEVNSQVGKGSTF 545 >lin2061 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 213 bits (543), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 0/427 (0%), Positives = 0/427 (0%), Gaps = 10/427 (2%) Query: 158 VMAGGLVLLFIAATFVSYKVASGMTSPLEQITRVARRITDLDYDARVPMNRRDEIGQLAT 217 Sbjct: 177 - 235 Query: 218 AINAMADSLQAQLKTIRDNEDLLQSVLDNMTGGIVMINAEGEIALLNRAAEQLLDVKNSE 277 Sbjct: 236 -- 293 Query: 278 MAGHSFKELKHHYELSRLIEEGVSRNEAVHEERSIYNPTERIVRLDGVPMIQDGSN-RGM 336 Sbjct: 294 ---- 349 Query: 337 LFLLQEVTEIRRLEKMRSEFVANVSHELKTPVAAVKGFAETLLGGGVKDEKTARSFLQII 396 Sbjct: 350 409 Query: 397 YDENERLNRLIGDILELSKIESKRAQLECSPVHLIEFFDSLLETLSKVAEKKNISLNASV 456 Sbjct: 410 469 Query: 457 -PEELFMEGDEDKLRQIFINLISNAINYTQDGGSXXXXXXXGQKADGTETVVFTVRDTGM 515 Sbjct: 470 529 Query: 516 GIPRKDLPRIFERFYRVDKAXXX-XXXXXXXXXXIVKHLVELHHGSISVESDLGIGSSFI 574 Sbjct: 530 589 Query: 575 LELPLLQ 581 Sbjct: 590 596 >BS_phoR_3 [T] COG0642 Signal transduction histidine kinase Length = 431 Score = 200 bits (509), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 16/262 (6%), Positives = 47/262 (17%), Gaps = 7/262 (2%) Query: 318 RIVRLDGVPMI-QDGSNRGMLFLLQEVTEIRRLEKMRSEFVANVSHELKTPVAAVKGFAE 376 + L + G L I + TP + Sbjct: 15 LLCCLPAFILGAFFGYLPWFLLASVTGLLIWHFWNLLRLSWWLWVDRSMTPPPGRGSWEP 74 Query: 377 TLLGGGVKDEKTARSFLQIIYDENERLNRLIGDILELSKIESKRAQLECSPVHLIEFFDS 436 L G + + ++ + + + C+ + Sbjct: 75 LLYGLHQMQLRNKKRRRELGNLIKRFRSGAESLPDAVVLTTEEGGIFWCNGLAQQILGLR 134 Query: 437 LLETLSKVAEKKNISLNASVPEE-LFMEGDEDKLRQIFINLISNAINYTQDGGSXXXXXX 495 E + + + + + +L + YT Sbjct: 135 WPEDNGQNILNLLRYPEFTQYLKTRDFSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDV 194 Query: 496 XGQKADGTETVVFTVRDTGMGIPRKDLPRIFERFYRVDKAXXXXXXXXXXXXXIVKHLVE 555 + R+ + + + ++ + + Sbjct: 195 TQMH-----QLEGARRNFFANVSHELRTPLTVLQGYLEMMNEQPLEGAVREKALHTMREQ 249 Query: 556 LHHGSISVESDLGIGSSFILEL 577 V+ L + Sbjct: 250 TQRMEGLVKQLLTLSKIEAAPT 271 >BS_resE_4 [T] COG0642 Signal transduction histidine kinase Length = 269 Score = 188 bits (478), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 101/255 (39%), Positives = 161/255 (63%), Gaps = 8/255 (3%) Query: 326 PMIQDGSNRGMLFLLQEVTEIRRLEKMRSEFVANVSHELKTPVAAVKGFAETLLGGGVKD 385 P+ + RG + +L+++TE RRL+K+R +F+ANVSHEL+TP++ ++G++E ++ Sbjct: 18 PLYAESHVRGAVAVLRDMTEERRLDKLREDFIANVSHELRTPISMLQGYSEAIVDDIASS 77 Query: 386 EKTARSFLQIIYDENERLNRLIGDILELSKIESKRAQLECSPVHLIEFFDSLLETLSKVA 445 E+ + QIIYDE+ R+ RL+ D+L+L+++ES L +++ EF + ++ S VA Sbjct: 78 EEDRKEIAQIIYDESLRMGRLVNDLLDLARMESGHTGLHYEKINVNEFLEKIIRKFSGVA 137 Query: 446 EKKNISLNA--SVPEELFMEGDEDKLRQIFINLISNAINYTQDGGSXXXXXXXGQKADGT 503 ++KNI+L+ S+ EE FM DEDK+ Q+F NLI NA+ +T GGS + DG Sbjct: 138 KEKNIALDHDISLTEEEFM-FDEDKMEQVFTNLIDNALRHTSAGGSVSISVHSVK--DGL 194 Query: 504 ETVVFTVRDTGMGIPRKDLPRIFERFYRVDKAXXXXXXXXXXXXXIVKHLVELHHGSISV 563 + ++D+G GIP +DLP IFERFY+ DKA IVK++VE H+GSI+V Sbjct: 195 K---IDIKDSGSGIPEEDLPFIFERFYKADKARTRGRAGTGLGLAIVKNIVEAHNGSITV 251 Query: 564 ESDLGIGSSFILELP 578 S + G++F +P Sbjct: 252 HSRIDKGTTFSFYIP 266 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,665,093 Number of extensions: 1490055 Number of successful extensions: 5085 Number of sequences better than 1.0e-05: 235 Number of HSP's gapped: 4694 Number of HSP's successfully gapped: 235 Length of query: 585 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 473 Effective length of database: 25,461,023 Effective search space: 12043063879 Effective search space used: 12043063879 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 119 (50.4 bits)