BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0005.p (280 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZmutM [L] COG0266 Formamidopyrimidine-DNA glycosylase 159 6e-39 ECs4510 [L] COG0266 Formamidopyrimidine-DNA glycosylase 159 6e-39 mutM [L] COG0266 Formamidopyrimidine-DNA glycosylase 159 7e-39 ZmutM [L] COG0266 Formamidopyrimidine-DNA glycosylase 159 7e-39 STM3726 [L] COG0266 Formamidopyrimidine-DNA glycosylase 158 7e-39 >ZmutM [L] COG0266 Formamidopyrimidine-DNA glycosylase Length = 269 Score = 159 bits (401), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 94/273 (34%), Positives = 139/273 (50%), Gaps = 5/273 (1%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVET +R + + G I + VR R+ + P + + L+ V+ V+RR K Sbjct: 1 MPELPEVETSRRGIEPHLVGATILHAVVRNGRL-RWPVSEEIY--RLSDQPVLSVQRRAK 57 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 +L L ++ HL M G + E P +KH HV ++G LRYTD R+FG Sbjct: 58 YLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAW--L 115 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 + F E + + K T IKP L++ + VVG+GNIY E+ Sbjct: 116 WTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASES 175 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L A IHP+ A +L+ ++ +LL I A L ++ GG+++K ++ G+ G + +L Sbjct: 176 LFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQ 235 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 +YGRK +PC CGT I T R T YC CQ Sbjct: 236 VYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQ 268 >ECs4510 [L] COG0266 Formamidopyrimidine-DNA glycosylase Length = 269 Score = 159 bits (401), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 94/273 (34%), Positives = 139/273 (50%), Gaps = 5/273 (1%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVET +R + + G I + VR R+ + P + + L+ V+ V+RR K Sbjct: 1 MPELPEVETSRRGIEPHLVGATILHAVVRNGRL-RWPVSEEIY--RLSDQPVLSVQRRAK 57 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 +L L ++ HL M G + E P +KH HV ++G LRYTD R+FG Sbjct: 58 YLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAW--L 115 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 + F E + + K T IKP L++ + VVG+GNIY E+ Sbjct: 116 WTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASES 175 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L A IHP+ A +L+ ++ +LL I A L ++ GG+++K ++ G+ G + +L Sbjct: 176 LFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQ 235 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 +YGRK +PC CGT I T R T YC CQ Sbjct: 236 VYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQ 268 >mutM [L] COG0266 Formamidopyrimidine-DNA glycosylase Length = 182 Score = 159 bits (401), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 0/273 (0%), Positives = 0/273 (0%), Gaps = 5/273 (1%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 M + + + K + + + + + Sbjct: 1 MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHL-AFSSWLFNA--KGQLLVTRRALSKKA 57 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 + + + L + R Sbjct: 58 WPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVE--N 115 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 + V + Sbjct: 116 EVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWMVMQATNREARKRL 175 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 Sbjct: 176 235 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 Sbjct: 236 268 Score = 159 bits (401), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 94/273 (34%), Positives = 139/273 (50%), Gaps = 5/273 (1%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVET +R + + G I + VR R+ + P + + L+ V+ V+RR K Sbjct: 1 MPELPEVETSRRGIEPHLVGATILHAVVRNGRL-RWPVSEEIY--RLSDQPVLSVQRRAK 57 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 +L L ++ HL M G + E P +KH HV ++G LRYTD R+FG Sbjct: 58 YLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAW--L 115 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 + F E + + K T IKP L++ + VVG+GNIY E+ Sbjct: 116 WTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASES 175 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L A IHP+ A +L+ ++ +LL I A L ++ GG+++K ++ G+ G + +L Sbjct: 176 LFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQ 235 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 +YGRK +PC CGT I T R T YC CQ Sbjct: 236 VYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQ 268 >STM3726 [L] COG0266 Formamidopyrimidine-DNA glycosylase Length = 269 Score = 158 bits (400), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 95/274 (34%), Positives = 142/274 (51%), Gaps = 7/274 (2%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVET +R + + G I + +R R+ + P + + L+ V+ V+RR K Sbjct: 1 MPELPEVETSRRGIEPHLVGATILHAHIRNGRL-RWPVSDEIY--RLSDTPVLSVQRRAK 57 Query: 61 FLRFVL-DGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHL 119 +L L DG +++ HL M G + P +KH HV ++G LRYTD R+FG Sbjct: 58 YLLLELPDGWIII-HLGMSGSLRILPEALPAEKHDHVDLVMSNGKILRYTDPRRFGAW-- 114 Query: 120 FQXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDE 179 + F E ++ + K T IKP L++ + VVG+GNIY E Sbjct: 115 LWTKELEGHNVLAHLGPEPLSDEFNGEYLQQKCAKKKTAIKPWLMDNKLVVGVGNIYASE 174 Query: 180 ALHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQL 239 +L A IHP+ A +L++ + DLL I A L ++ GG+++K ++ G+ G + +L Sbjct: 175 SLFAAGIHPDRLASSLSTEECDLLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQEL 234 Query: 240 LIYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 +YGRK +PC CGT I T R T YC CQ Sbjct: 235 QVYGRKGEPCRVCGTPIVATKHAQRATFYCRHCQ 268 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,920,519 Number of extensions: 797843 Number of successful extensions: 2880 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 2842 Number of HSP's successfully gapped: 20 Length of query: 280 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 175 Effective length of database: 26,268,361 Effective search space: 4596963175 Effective search space used: 4596963175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)