BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0271.0004.p
         (253 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BH3151 [S] COG1971 Predicted membrane protein                          96   4e-20
BS_ytaF [S] COG1971 Predicted membrane protein                         74   2e-13

>BH3151 [S] COG1971 Predicted membrane protein
          Length = 213

 Score = 96.3 bits (238), Expect = 4e-20,   Method: Compositional matrix adjust.
 Identities = 68/234 (29%), Positives = 104/234 (44%), Gaps = 38/234 (16%)

Query: 20  DGFGVGITYGLRKMKXXXXXXXXXXXXXXXXXYVSMQVGVLLAKVVSPHAASSIGAVILV 79
           D FGVG+TYGLRKMK                  ++M  G+ + + +SP  A S+G +IL+
Sbjct: 17  DSFGVGLTYGLRKMKIPFKSLLFIAGCSAVSILIAMGFGMTIQQYLSPAIAESLGGIILI 76

Query: 80  LMGCWSLFQMLTQKEKDHDGKSPGDQHGRADHDXXXXXXXXXXXXXXXXXXXXXXXXXSP 139
           L+GCW+L+Q     +  H  K   D H                                 
Sbjct: 77  LIGCWALYQAFRPAKNGHKTK---DDH--------------------------------- 100

Query: 140 KSAVFSLELRHLGVVIQILRTPSSADMDASGSISSTEAMVLGIXXXXXXXXXXXXXXXXX 199
              + + E++ LGVVI+ILR P  AD D SG+I+  EA++LGI                 
Sbjct: 101 --VILNFEIKMLGVVIRILRKPMVADFDNSGAITGREAVILGIALSLDAFGAGIGAALIG 158

Query: 200 XXPVSTSLMIALFSGTFLLLGMKTGLKLSGSYWMKHXXXXXXXXXXXMGIMKLL 253
             P+  ++ +A+ S  F+ LGMK+G + + SYW+K            +G+  L+
Sbjct: 159 FHPLYMAVSVAVMSALFVSLGMKSGYRFADSYWIKRFSFIPGLLLVLIGVFNLM 212


>BS_ytaF [S] COG1971 Predicted membrane protein
          Length = 159

 Score = 74.3 bits (181), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 49/162 (30%), Positives = 66/162 (40%), Gaps = 38/162 (23%)

Query: 20  DGFGVGITYGLRKMKXXXXXXXXXXXXXXXXXYVSMQVGVLLAKVVSPHAASSIGAVILV 79
           D F VG TYGLRKMK                 ++SM +G  L K    +    +G +ILV
Sbjct: 14  DSFSVGFTYGLRKMKIPFKAILVIACCSGAVMFISMLIGSFLTKFFPVYVTEKLGGLILV 73

Query: 80  LMGCWSLFQMLTQKEKDHDGKSPGDQHGRADHDXXXXXXXXXXXXXXXXXXXXXXXXXSP 139
            +G W L+Q             P        H+                           
Sbjct: 74  GIGAWVLYQFF----------KPAKDKEYLLHE--------------------------- 96

Query: 140 KSAVFSLELRHLGVVIQILRTPSSADMDASGSISSTEAMVLG 181
              + +LE+R LG+VI ILR P SAD+D SG I+  EA++LG
Sbjct: 97  -KTLLNLEVRSLGIVIHILRKPMSADIDKSGVINGIEAVLLG 137


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.321    0.136    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 10,602,054
Number of extensions: 250736
Number of successful extensions: 316
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 314
Number of HSP's successfully gapped: 4
Length of query: 253
Length of database: 38,378,431
Length adjustment: 104
Effective length of query: 149
Effective length of database: 26,383,695
Effective search space: 3931170555
Effective search space used: 3931170555
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 114 (48.5 bits)