BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0270.0009.p (459 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MT0807 [GEPR] COG0477 Permeases of the major facilitator superfa... 116 1e-25 Rv0783c [GEPR] COG0477 Permeases of the major facilitator superf... 115 1e-25 CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator supe... 105 1e-22 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 92 2e-18 XF1765 [GEPR] COG0477 Permeases of the major facilitator superfa... 92 2e-18 >MT0807 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 545 Score = 116 bits (290), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 103/352 (29%), Positives = 171/352 (48%), Gaps = 26/352 (7%) Query: 8 LLISVGLGVL---LNPLNSSMISVAIARLQNVYRLDFTE----VSWIIFSFYIASAVAQP 60 LL + G+ VL + ++ +++SVA Q + DF V+W + + +A A P Sbjct: 46 LLRTAGVCVLASVMAHVDVTVVSVA----QRTFVADFGSTQAVVAWTMTGYMLALATVIP 101 Query: 61 IMGKASDLFGRRKIFLAGLVVAFVASLAAPLAPGFGWLIVFRIVQSIGTSMMVSVGMAIV 120 G A+D FG R++F+ ++ + SL +AP LI+FR+VQ G M+ V AI+ Sbjct: 102 TAGWAADRFGTRRLFMGSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAIL 161 Query: 121 RVHITEKQ-ASALSVMSIFLSGAAAIGPFIGGVVIHWWDWHGIFFVNIPFAAASFLLAWR 179 K+ ++V+ I + GP +GG +I + W IF VN+P ++ +LA Sbjct: 162 AREAGPKRLGRVMAVVGIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAI 221 Query: 180 AIPKDEPPVAALRNMSFRQWITMIDAPGIXXXXXXXXXXXXXXXXXXXXXHISFGHVLTG 239 P+D P AA N + + + +PG+ ++ HVL Sbjct: 222 VFPRDRP--AASENFDYMGLLLL--SPGLATFLFGVSSSPARGT-------MADRHVLIP 270 Query: 240 GI-GLIALVAFVRHE-LKASSPFIPVRTFAKYPEMARVNVEFILVNVLYYSLFFGLPSYL 297 I GL + AFV H + P I +R F +A+ N+ ++++ + F LPSYL Sbjct: 271 AITGLALIAAFVAHSWYRTEHPLIDMRLFQNR-AVAQANMTMTVLSLGLFGSFLLLPSYL 329 Query: 298 QIVRHVSEFHTGILMLSLGLCSLIASPIAGRWIDRSGPGPALFLSAILMALG 349 Q V H S +G+ ++ GL +++A PIAG +DR GP + + +L+A G Sbjct: 330 QQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRRGPAKIVLVGIMLIAAG 381 >Rv0783c [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 540 Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 103/352 (29%), Positives = 171/352 (48%), Gaps = 26/352 (7%) Query: 8 LLISVGLGVL---LNPLNSSMISVAIARLQNVYRLDFTE----VSWIIFSFYIASAVAQP 60 LL + G+ VL + ++ +++SVA Q + DF V+W + + +A A P Sbjct: 41 LLRTAGVCVLASVMAHVDVTVVSVA----QRTFVADFGSTQAVVAWTMTGYMLALATVIP 96 Query: 61 IMGKASDLFGRRKIFLAGLVVAFVASLAAPLAPGFGWLIVFRIVQSIGTSMMVSVGMAIV 120 G A+D FG R++F+ ++ + SL +AP LI+FR+VQ G M+ V AI+ Sbjct: 97 TAGWAADRFGTRRLFMGSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAIL 156 Query: 121 RVHITEKQ-ASALSVMSIFLSGAAAIGPFIGGVVIHWWDWHGIFFVNIPFAAASFLLAWR 179 K+ ++V+ I + GP +GG +I + W IF VN+P ++ +LA Sbjct: 157 AREAGPKRLGRVMAVVGIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAI 216 Query: 180 AIPKDEPPVAALRNMSFRQWITMIDAPGIXXXXXXXXXXXXXXXXXXXXXHISFGHVLTG 239 P+D P AA N + + + +PG+ ++ HVL Sbjct: 217 VFPRDRP--AASENFDYMGLLLL--SPGLATFLFGVSSSPARGT-------MADRHVLIP 265 Query: 240 GI-GLIALVAFVRHE-LKASSPFIPVRTFAKYPEMARVNVEFILVNVLYYSLFFGLPSYL 297 I GL + AFV H + P I +R F +A+ N+ ++++ + F LPSYL Sbjct: 266 AITGLALIAAFVAHSWYRTEHPLIDMRLFQNR-AVAQANMTMTVLSLGLFGSFLLLPSYL 324 Query: 298 QIVRHVSEFHTGILMLSLGLCSLIASPIAGRWIDRSGPGPALFLSAILMALG 349 Q V H S +G+ ++ GL +++A PIAG +DR GP + + +L+A G Sbjct: 325 QQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRRGPAKIVLVGIMLIAAG 376 >CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 458 Score = 105 bits (263), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 95/414 (22%), Positives = 189/414 (45%), Gaps = 24/414 (5%) Query: 1 MNARSRWL-LISVGLGVLLNPLNSSMISVAIARLQNVYRLDFTEVSWIIFSFYIASAVAQ 59 MN R +++ I + + L ++S+++++A+ + + +++ + SWI + +A AV Sbjct: 1 MNERKKYIGFIGILIAAFLGVIDSTIVNIALPNITDYFKVSLNDTSWISTIYALALAVFM 60 Query: 60 PIMGKASDLFGRRKIFLAGLVVAFVASLAAPLAPGFGWLIVFRIVQSIGTSMMVSVGMAI 119 K +D FGR+K+ + G+++ V+S ++ +LI+ R VQ IG +++ + + + Sbjct: 61 ITAAKFADQFGRKKLMIIGILIFGVSSALCGISNSLLFLIIMRFVQGIGGAIITPIALPM 120 Query: 120 -VRVHITEKQASALSVMSIFLSGAAAIGPFIGGVVIHWWDWHGIFFVNIPFAAASFLLAW 178 + + E+ + + AAA GP +GGV+I + +W +FFVN+PFA A+ +L Sbjct: 121 GIEIFGKERMNIVVGASGAVVGLAAASGPPLGGVLIKYINWQSVFFVNVPFAIAALIL-- 178 Query: 179 RAIPKDEPPVAALRNMSFRQWITM-IDAPGIXXXXXXXXXXXXXXXXXXXXXHISFGHVL 237 + N S+ ++ ID G+ S + Sbjct: 179 ---------IVICVNESYDNTVSKDIDWFGVMTLTISLFCLIFALLKGKDYGWTSTLIIS 229 Query: 238 TGGIGLIALVAFVRHELKASSPFIPVRTFAKYPEMARVNVEFILVNVLYYSLFFGLPSYL 297 I +++L+ F+ ELK SSP I + F K P ++ +++ L YL Sbjct: 230 LFLIFVVSLILFIIIELKVSSPMIEINLF-KEPTFTASSICYMITGFGLMCPLLILNYYL 288 Query: 298 QIVRHVSEFHTGILMLSLGLCSLIASPIAGRWIDRSGPGPALFLSAILMALG----SVWI 353 Q V +M+S+ L + ++PI + P F+ + + +G S Sbjct: 289 QNVLQYDTLEAAFIMMSVSLTGMFSTPIGSILGKKVSPRIINFIGMLSLGIGIFRLSYLS 348 Query: 354 VTMNQSSPVISVCVALGAFGIGNGLNSVGMQAALFKSAPKEIIGVASGVFMTSR 407 M++ + + + + GIG G + + +++ K PKE G+ SG+ +R Sbjct: 349 YGMSKGTMIFDLIIC----GIGLGFSVQALSSSV-KFIPKEKSGIGSGIVNAAR 397 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 91.7 bits (226), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 92/401 (22%), Positives = 168/401 (41%), Gaps = 13/401 (3%) Query: 2 NARSRWLLI-SVGLGVLLNPLNSSMISVAIARLQNVYRLDFTEVSWIIFSFYIASAVAQP 60 N +W+++ +V G LN+SM++ AI +L NV+ D W+I F +A + P Sbjct: 3 NIPKKWMVVCAVLFGSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMP 62 Query: 61 IMGKASDLFGRRKIFLAGLVVAFVASLAAPLAPGFGWLIVFRIVQSIGTSMMVSVGMAIV 120 + G D G+++ ++ GL + + SL L+ LIVFR +Q IG +M+ + M ++ Sbjct: 63 LTGYLGDKIGKKEAYILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMTLI 122 Query: 121 -RVHITEKQASALSVMSIFLSGAAAIGPFIGGVVIHWWDWHGIFFVNIPFAAASFLLAWR 179 ++ A V + A IGP +GG ++ +W +F VNIPF + A Sbjct: 123 FDAFPRNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVI 182 Query: 180 AIPKDEPPVAALRNMSFRQWITMIDAPGIXXXXXXXXXXXXXXXXXXXXXHISFGHVLTG 239 +PK +A + +W + G+ + Sbjct: 183 YLPK----IARSGQIKLDRWGFLFVTAGVGSILLAFGRMSDLTHLTEWINGVLLLLGAL- 237 Query: 240 GIGLIALVAFVRHELKASSPFIPVRTFAKYPEMARVNVEFILVNVLYYSLFFGLPSYLQI 299 L FV+ E +A P + + F + V I L+ +F +P LQ Sbjct: 238 -----CLYVFVQVEKRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGIFL-VPLLLQQ 291 Query: 300 VRHVSEFHTGILMLSLGLCSLIASPIAGRWIDRSGPGPALFLSAILMALGSVWIVTMNQS 359 V TG++ L L + + I GR +D+ GP + ++ A+G+ + ++ Sbjct: 292 VYDYGPIMTGLVFLPSALFTGLTMSIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLE 351 Query: 360 SPVISVCVALGAFGIGNGLNSVGMQAALFKSAPKEIIGVAS 400 + + + + G+G GL ++ + P+ +I S Sbjct: 352 TGLWYIFAWMAIRGVGMGLTTMPATTTGMNAIPEGLISRGS 392 >XF1765 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 472 Score = 91.7 bits (226), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 112/423 (26%), Positives = 184/423 (43%), Gaps = 51/423 (12%) Query: 4 RSRWLLISVGLGVLLNPLNSSMISVAIARLQNVYRLDFTEVSWIIFSFYIASAVAQPIMG 63 R++ LI+V LG + L++ +++VA+ L + + W++ + + G Sbjct: 24 RAQATLIAVLLGFFVITLDAVVVNVALPTLAHDLGASVDGLQWVVDGYTLMFGALLLSAG 83 Query: 64 KASDLFGRRKIFLAGLVVAFVASLAAPLAPGFGWLIVFRIVQSIGTSMMVSVGMAIVRVH 123 D G R+ F GLVV +AS+A AP L+V R+VQ G ++M+ M+++R Sbjct: 84 ALVDRIGARRAFGLGLVVFILASMACGGAPTLATLVVARLVQGAGAAVMMPASMSLIRQA 143 Query: 124 ITE--KQASALSVMSIFLSGAAAIGPFIGGVVIHWWDWHGIFFVNIPFA--AASFLLAWR 179 + ++ A+++ ++ S A+ GP IGG ++ DW IFFVN+P +FL Sbjct: 144 YPDPVRRGHAVALWAMGGSVASTSGPVIGGWLVS-LDWRWIFFVNLPVGLLTLAFLARTP 202 Query: 180 AIPKDEPPVAALRNMSFRQWITMIDAPGIXXXXXXXXXXXXXXXXXXXXXHISFGHVLTG 239 A P+ P A+ +T I A G + +G + G Sbjct: 203 ASPRRASPFDAVGQ------VTAILAMG----------------------ALIYGAIEAG 234 Query: 240 GIGLIALVAFVR-------------HELKASSPFIPVRTFAKYPEMARVN--VEFILVNV 284 GL A + + P +P A P AR++ V F + V Sbjct: 235 AQGLGATPVLAAFVLALAALAAFVWSQRCGAHPMVPPGLLA--PRNARISMAVGFTFM-V 291 Query: 285 LYYSLFFGLPSYLQIVRHVSEFHTGILMLSLGLCSLIASPIAGRWIDRSGPGPALFLSAI 344 Y+ L F + YLQ R +S TGI L + L L+ +P + R R G + + Sbjct: 292 GYFGLPFVMSLYLQQHRGLSAPATGIAFLPMMLIGLVLTPFSARLAHRFGARTMIVSGLL 351 Query: 345 LMALGSVWIVTMNQSSPVISVCVALGAFGIGNGLNSVGMQAALFKSAPKEIIGVASGVFM 404 MA+G + ++ S+PV + + G+ + + A L S P + G ASGVF Sbjct: 352 SMAVGLAAVASLPASAPVGWIAALMVLVGLSGPFVAPPVAAVLLSSVPGALAGTASGVFN 411 Query: 405 TSR 407 TSR Sbjct: 412 TSR 414 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.141 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,126,897 Number of extensions: 1127160 Number of successful extensions: 3862 Number of sequences better than 1.0e-05: 87 Number of HSP's gapped: 3831 Number of HSP's successfully gapped: 94 Length of query: 459 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 349 Effective length of database: 25,691,691 Effective search space: 8966400159 Effective search space used: 8966400159 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)