BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0270.0003.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1961 [R] COG1409 Predicted phosphohydrolases 181 1e-45 CAC2806 [R] COG1409 Predicted phosphohydrolases 177 2e-44 YLR361c [R] COG1409 Predicted phosphohydrolases 113 5e-25 YOR137c [R] COG1409 Predicted phosphohydrolases 65 1e-10 BS_ykuE [R] COG1408 Predicted phosphohydrolases 51 2e-06 >CAC1961 [R] COG1409 Predicted phosphohydrolases Length = 324 Score = 181 bits (459), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 103/300 (34%), Positives = 158/300 (52%), Gaps = 20/300 (6%) Query: 1 MNKQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLVY 60 + ++L F G FKIVQF D+H D I + +T+ ME+++ E+PD V+ +GD + Sbjct: 31 LQNKKLKFDNCGKFKIVQFADLHQNDCI----NLKTVHFMEKVMDYEKPDFVILTGDNID 86 Query: 61 SEETVDPKATFRRVIEVAV----QAGTPFAVIYGNHDTEK-GVTREELQAILTEYELCMA 115 +D T+ + IE V + P+A + GNHDTE V R+ + +Y+ M Sbjct: 87 GRYCMD--ITYEKAIESVVRPIEERRIPWAAVLGNHDTESLQVERKNMIKNYMKYKYNMN 144 Query: 116 EAGPGEIHGTGNYVLPLFNHSADRESAALYFVDSGEYAPAAIGGYAWIHSDQVAWYLQES 175 + I + L + + +Y +DSG Y+ GGY I +V WY + Sbjct: 145 KITDDGIQ----FNLLVMDSENKNPIFNMYMLDSGSYSKK--GGYGCIEPYEVKWYKKTV 198 Query: 176 AKNRERNGGVLPALAFMHIPIPEYQEVWQSGSVAGRKGEMVCCSKVNSGWFAAMLEAGDV 235 +++ G ++PA F HIPI +Y E W++ + G K E +C ++G F M + DV Sbjct: 199 TDLKKKYGHIVPAFMFFHIPIIQYNEAWENEKLCGEKREKICHQSTDNGLFKEMQKEKDV 258 Query: 236 MATFAGHDHDNDYIGVLHGITLAYGRVTGHNTY--GSLQRGARVIQLKEGE-RRFETWIR 292 A F GHDH N++IG GI + YGR TG++TY + +RGARVI L E +F+TW R Sbjct: 259 KAIFVGHDHTNNFIGRNKGIIMGYGRCTGYDTYDASNYERGARVIYLDEDNINKFKTWER 318 >CAC2806 [R] COG1409 Predicted phosphohydrolases Length = 317 Score = 177 bits (450), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 109/296 (36%), Positives = 158/296 (53%), Gaps = 18/296 (6%) Query: 3 KQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLV--- 59 K +++F +G FKIVQFTD+H ++ T+ LME IL SE+P+LVV +GD V Sbjct: 27 KFKVAF-KEGKFKIVQFTDLHE----HAVKNEYTIKLMENILDSEKPNLVVITGDCVDGR 81 Query: 60 YSEETVDPKATFRRVIEVAVQAGTPFAVIYGNHDTEKG-VTREELQAILTEYELCMAEAG 118 Y + K + + P+AV GNHD+E V+RE I Y+ +++ Sbjct: 82 YCNGEKEVKGVIDNIAKPMEDRRIPWAVTLGNHDSEACQVSRERQMEIYMSYKYNLSDKF 141 Query: 119 PGEIHGTGNYVLPLFNHSADRESAALYFVDSGEYAPAAIGGYAWIHSDQVAWYLQESAKN 178 G+Y + + + + ++ LY +DSG Y GY ++ +Q+AWY +++A N Sbjct: 142 STVSDKAGDYNIVIQDEN-NKPVYNLYMLDSGSYTK---DGYGYVEKEQIAWY-EDTANN 196 Query: 179 RERNGGV-LPALAFMHIPIPEYQEVWQSGSVAGRKGEMVCCSKVNSGWFAAMLEAGDVMA 237 ++ +P+L F HIP+ + EVWQSG G + E CC ++G F+ + E GDV Sbjct: 197 LKKCFQTRIPSLMFFHIPLKQQYEVWQSGKAVGERNENECCQGEDTGLFSKLKEIGDVKG 256 Query: 238 TFAGHDHDNDYIGVLHGITLAYGRVTGHNTYGS--LQRGARVIQLKEGE-RRFETW 290 F GHDH NDY G L GI L YGR TG N Y +GARVI L E F T+ Sbjct: 257 VFVGHDHTNDYWGSLDGIALCYGRKTGFNCYDKEGFIKGARVIVLNENHLEEFNTY 312 >YLR361c [R] COG1409 Predicted phosphohydrolases Length = 578 Score = 113 bits (282), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 83/272 (30%), Positives = 128/272 (47%), Gaps = 32/272 (11%) Query: 4 QRLSFHADGTFKIVQFTDIHLKDGIEPE------------RDTRTLALMERILISEQPDL 51 QRL +G FKIVQ D+HL G E E D +T ++++L E+P L Sbjct: 238 QRLQETDEGKFKIVQLADLHLGVG-ESECIDEYPKHEACKADPKTETFVQQVLDIEKPQL 296 Query: 52 VVFSGDLVYSEETV-DPKATFRRVIEVAVQAGTPFAVIYGNHDTEKGVTREELQAILTEY 110 VVF+GD + + ++ D + + + + P+A+++GNHD E +TR +L I + Sbjct: 297 VVFTGDQIMGDRSIQDSETVLLKAVAPVIARKIPWAMVWGNHDDEGSLTRWQLSEIASVL 356 Query: 111 ELCMAEAGPGEIH----GTGNYVLPLF-NHSADRESAALYFVDSGEYAPAA--IGGYAWI 163 + + P + H G GNY+ +F N+ + LYF+DS +Y+ GY WI Sbjct: 357 PYSLFKFSPHDTHDNTFGVGNYIYQIFSNNDTEVPVGTLYFLDSHKYSTVGKIYPGYDWI 416 Query: 164 HSDQVAWYLQESAKNRERNGGVLPALAFMHIPIPEYQEVWQSGSVAGRKGEM-------V 216 Q + N + G+ ++AF HIP+PEY + +S + G K + V Sbjct: 417 KESQWKYIEDYHDVNLKFKTGL--SMAFFHIPLPEYLNI-ESKTHPGEKNPLIGMYKEGV 473 Query: 217 CCSKVNSGWFAAMLEAGDVMATFAGHDHDNDY 248 K NS L+ V GHDH NDY Sbjct: 474 TAPKYNSEGITT-LDRLSVDVVSCGHDHCNDY 504 >YOR137c [R] COG1409 Predicted phosphohydrolases Length = 622 Score = 64.7 bits (156), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 51/203 (25%), Positives = 88/203 (43%), Gaps = 32/203 (15%) Query: 13 TFKIVQFTDIHLK---DGIEPERDTRTLALMERILISEQPDLVVFSGDLVYSEETVDPKA 69 +FKI+Q TD H K + + + +T+ ++R+L SE PDLVV +GDL+ S T+D + Sbjct: 309 SFKILQITDFHFKCTDNSMTVINEIKTVNFIDRVLASENPDLVVITGDLLDSHNTIDYQT 368 Query: 70 TFRRVIEVAVQAGTPFAVIYGNHDTEKGVTREELQAILTEYELCMAEAGPGEIHG----- 124 +V++ + P+A+ G D T +++ + E H Sbjct: 369 CIMKVVQPMISNKIPYAISLGVSDESNLATSAQIRDFIRNLPYTFNNVASEEGHMAIEVS 428 Query: 125 -----TGNYVLPLFNHSAD--RESAALYFVDSGEYAPAAIGGYAWIHSDQVAWYLQESAK 177 T N +L + D S AL+FV +AP + + ++D + Sbjct: 429 FKKKLTKNTLLERDIDTEDETNPSEALFFV-FDSFAP--VNNFLQDYNDLI--------- 476 Query: 178 NRERNGGVLPALAFMHIPIPEYQ 200 G + LAF + P+ EY+ Sbjct: 477 -----GKIDFGLAFQYFPLSEYR 494 >BS_ykuE [R] COG1408 Predicted phosphohydrolases Length = 287 Score = 51.2 bits (121), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 35/93 (37%), Positives = 48/93 (51%), Gaps = 9/93 (9%) Query: 3 KQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLVYSE 62 K L H FKIVQF+D HL D E D +T+ L + +PDL+VF+GD++ Sbjct: 47 KSSLIPHGFDGFKIVQFSDAHLSDYFTLE-DLKTVIL---TINESKPDLIVFTGDII--- 99 Query: 63 ETVDPKATFRRVIEVAVQAGTPFA--VIYGNHD 93 + D + VI + + PF IYGNHD Sbjct: 100 DNPDTYQHHQAVIPLLRKLNAPFGKLCIYGNHD 132 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,660,843 Number of extensions: 967720 Number of successful extensions: 1706 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1693 Number of HSP's successfully gapped: 5 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)