BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0269.0012.p (533 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP0661 [T] COG4753 Response regulator containing CheY-like recei... 119 1e-26 BH2728 [T] COG4753 Response regulator containing CheY-like recei... 113 8e-25 BH2109 [T] COG4753 Response regulator containing CheY-like recei... 110 4e-24 SPy1556 [T] COG4753 Response regulator containing CheY-like rece... 108 2e-23 BH1910 [T] COG4753 Response regulator containing CheY-like recei... 100 4e-21 >SP0661 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 245 Score = 119 bits (298), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 50/123 (40%), Positives = 86/123 (69%) Query: 5 IMLVDDEILIRENIRECIDWEKEGFHYCGDAPDGELALPIIEAQLPDILITDIKMPFMNG 64 I++V+DE L+R+ + + ++ G A +G A +I+ Q+PDI++TDI MP +NG Sbjct: 5 ILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWELIQKQVPDIILTDINMPHLNG 64 Query: 65 LELTSVVRQKFPQIKIIILSGHDDFQYAQKALRLGVEDYCLKPFSAADLLQLLRSVSARI 124 ++L S+VR+ +PQ+ ++ L+G+DDF YA A++LGV+DY LKPFS D+ ++L + ++ Sbjct: 65 IQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKIKQKL 124 Query: 125 DEE 127 D+E Sbjct: 125 DKE 127 Score = 55.8 bits (133), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 33/95 (34%), Positives = 54/95 (56%) Query: 421 QYIREQYDKEQLSLNDISKVVRVSPSHLSKTFSQATGQTITEFLTATRMDRAKELLKSTG 480 Q I+ Q SL ++ + SP++LS + G ++L R+ +AK LL +T Sbjct: 147 QKIQSHLADSQFSLKSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTD 206 Query: 481 HKTFEIAYLVGYNDQHYFSNLFKKVTGMTPMEFRK 515 K +EIA VG+ D +YF+ FK++ G+TP +F+K Sbjct: 207 LKIYEIAEKVGFEDMNYFTQRFKQIAGVTPRQFKK 241 >BH2728 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 510 Score = 113 bits (283), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 54/124 (43%), Positives = 80/124 (64%), Gaps = 1/124 (0%) Query: 2 MKKIMLVDDEILIRENIRECIDWEKEGFHYCGDAPDGELALPIIEAQLPDILITDIKMPF 61 M +++VDDE LIR+ I DWEKEGF G+A +G+ AL +I P I++TDI MP Sbjct: 1 MCNVLIVDDEKLIRQGIINFFDWEKEGFQIVGEAANGKDALELISKNEPHIVMTDIVMPV 60 Query: 62 MNGLELTSVVRQKFPQIKIIILSGHDDFQYAQKALRLGVEDYCLKP-FSAADLLQLLRSV 120 M+G++L V++ FP+++II+LS ++F+Y + GV DY LKP + DLL+ L V Sbjct: 61 MDGIDLVKRVKRDFPEVEIIVLSSFEEFEYVRSTFHSGVADYILKPRLNEKDLLKTLSEV 120 Query: 121 SARI 124 +I Sbjct: 121 RGKI 124 Score = 77.8 bits (190), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 46/139 (33%), Positives = 76/139 (54%), Gaps = 5/139 (3%) Query: 386 SSDDGLQYLKRLYERLWEWRSEGADKHRELIDRVKQYIREQYDKEQLSLNDISKVVRVSP 445 ++D Q+L+ + + + +EG+ I ++ YI E++ E LSL+ ++ +P Sbjct: 376 ATDCFYQFLEVTKQIISVYSNEGSSS----IQKMLAYI-EEHHSEPLSLSQLANYFHFNP 430 Query: 446 SHLSKTFSQATGQTITEFLTATRMDRAKELLKSTGHKTFEIAYLVGYNDQHYFSNLFKKV 505 S+LS F Q + +E+L R+ +AKE LKS+ EI LVGY+D YF +FKK Sbjct: 431 SYLSAYFRQHHDEGFSEYLNKVRIRKAKEWLKSSTIPISEIYALVGYSDHSYFCKVFKKR 490 Query: 506 TGMTPMEFRKQGQTEDQLH 524 TGM P +R++ + H Sbjct: 491 TGMPPSRYRRENAEHHETH 509 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 110 bits (276), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 51/127 (40%), Positives = 79/127 (62%), Gaps = 1/127 (0%) Query: 2 MKKIMLVDDEILIRENIRECIDWEKEGFHYCGDAPDGELALPIIEAQLPDILITDIKMPF 61 M K++L DDE +R ++ I+WEK G+ +A +GE A +++ PD++ITDI+MP Sbjct: 1 MYKVLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPV 60 Query: 62 MNGLELTSVVRQKFP-QIKIIILSGHDDFQYAQKALRLGVEDYCLKPFSAADLLQLLRSV 120 +GL+L V + K II+SG++DF YAQKA+R G D+ LKP +L L + Sbjct: 61 FDGLQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKL 120 Query: 121 SARIDEE 127 +A +D+E Sbjct: 121 AATLDQE 127 Score = 71.6 bits (174), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 65/300 (21%), Positives = 135/300 (45%), Gaps = 26/300 (8%) Query: 231 QMNQALDELCAAAKQRLREVCGLELSVSRGKVCERLQGIHLSYLEAENDRMFKQMSRMHS 290 + N ++ L ++ +++ + L+V G + L I SY A + R +K + + Sbjct: 235 KFNGQIEPLAEKLRKDIQDFTNIPLTVYVGAKTDDLSTIKKSYETANSLRNYKYIFNEKN 294 Query: 291 AAM------LEVYYDPSANGVLLDRSRLVQFLKSGDHRQMSDFLLELSTELERMNWNSGY 344 + ++ YD N + + L++ ++ + ++ + + ++ +E + + Sbjct: 295 VFIHEQTESVDTTYDELNNHLFV---TLMEQMEENNSDRIVNIIDKIFSEFQTKKFVPSA 351 Query: 345 ACYLMNDITLELVQTAKNGFRAAAGHAGILQELQTQLKQISSSDDGLQYLKRLYER---- 400 +N +++QT +A G G + Q + S + LK+++ + Sbjct: 352 VKTSVNRCVHKVMQTV----QAMEGDTGKIISAQA-MTNWESYNLTFDQLKQVFTKFVLE 406 Query: 401 ----LWEWRSEGADKHRELIDRVKQYIREQYDKEQLSLNDISKVVRVSPSHLSKTFSQAT 456 + E R E + I +VK YI Y E +SL I+ + ++P ++ + F + Sbjct: 407 AAVIIQELRKENM---KSDIYKVKTYIENHY-HENISLKSIASLFYMNPVYMGQLFKKTF 462 Query: 457 GQTITEFLTATRMDRAKELLKSTGHKTFEIAYLVGYNDQHYFSNLFKKVTGMTPMEFRKQ 516 G E+L RM+ AK+LL+ T + +E+A VG+ YF F+K+ MTP E+R + Sbjct: 463 GVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTDYFVTQFEKINKMTPTEYRNK 522 >SPy1556 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 246 Score = 108 bits (270), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 47/124 (37%), Positives = 83/124 (66%) Query: 2 MKKIMLVDDEILIRENIRECIDWEKEGFHYCGDAPDGELALPIIEAQLPDILITDIKMPF 61 M +++V+DE L+R+ IR +D+ + +A +G+LA + + + DI++TDI MP Sbjct: 1 MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLFQKEPYDIVLTDINMPK 60 Query: 62 MNGLELTSVVRQKFPQIKIIILSGHDDFQYAQKALRLGVEDYCLKPFSAADLLQLLRSVS 121 +NG++L +++Q+ PQ ++ L+G+DDF YA AL+LG +DY LKPFS AD+ +L + Sbjct: 61 LNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDYLLKPFSKADVEDMLGKLR 120 Query: 122 ARID 125 +++ Sbjct: 121 KKLE 124 Score = 58.9 bits (141), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 33/97 (34%), Positives = 57/97 (58%) Query: 423 IREQYDKEQLSLNDISKVVRVSPSHLSKTFSQATGQTITEFLTATRMDRAKELLKSTGHK 482 I E+ L+L +++ + SP++LS + G ++L R+ +AK LL ++ K Sbjct: 150 IHERLADSDLTLKSLAQQLGFSPNYLSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLK 209 Query: 483 TFEIAYLVGYNDQHYFSNLFKKVTGMTPMEFRKQGQT 519 +EIA VG+ D +YFS FK++ G+TP +++K GQ Sbjct: 210 IYEIAEQVGFEDMNYFSQRFKQLVGVTPSQYKKGGQA 246 >BH1910 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 506 Score = 100 bits (250), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 50/123 (40%), Positives = 71/123 (57%) Query: 2 MKKIMLVDDEILIRENIRECIDWEKEGFHYCGDAPDGELALPIIEAQLPDILITDIKMPF 61 M +M+VDDE ++RE ++ I WE+ GF A +G+ PD++I DI+MP Sbjct: 1 MYDVMIVDDEAMVREGLKALISWEEYGFRVIDTARNGKEGFTKYRQLSPDLMIVDIRMPE 60 Query: 62 MNGLELTSVVRQKFPQIKIIILSGHDDFQYAQKALRLGVEDYCLKPFSAADLLQLLRSVS 121 ++GL L +R + I IILSGH DF YA+KAL +DY LKP +L+ L+ V Sbjct: 61 VDGLSLIESIRAEDSAIHFIILSGHSDFNYAKKALGCQCDDYLLKPLDEDELIDSLKKVY 120 Query: 122 ARI 124 RI Sbjct: 121 GRI 123 Score = 59.7 bits (143), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 32/94 (34%), Positives = 51/94 (54%), Gaps = 2/94 (2%) Query: 423 IREQYDKEQLSLNDISKVVRVSPSHLSKTFSQATGQTITEFLTATRMDRAKELLKSTGHK 482 I + Y K L L ++ V + ++L K F TG+ +L R++ K LL+ G+K Sbjct: 406 IHKDYAK-NLRLETLASVFNYNRAYLGKLFKNYTGEYFNTYLDKVRIENGKRLLQK-GYK 463 Query: 483 TFEIAYLVGYNDQHYFSNLFKKVTGMTPMEFRKQ 516 +E+A +GY + YF FKK G++P FRK+ Sbjct: 464 VYEVAEKIGYANVDYFHRKFKKYVGVSPSTFRKR 497 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,404,148 Number of extensions: 1337483 Number of successful extensions: 10853 Number of sequences better than 1.0e-05: 396 Number of HSP's gapped: 10912 Number of HSP's successfully gapped: 407 Length of query: 533 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 422 Effective length of database: 25,576,357 Effective search space: 10793222654 Effective search space used: 10793222654 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)