BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0269.0004.p (641 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1908 [G] COG1472 Beta-glucosidase-related glycosidases 53 2e-06 BH3231 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 51 6e-06 >BH1908 [G] COG1472 Beta-glucosidase-related glycosidases Length = 926 Score = 52.8 bits (125), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 29/89 (32%), Positives = 41/89 (46%), Gaps = 1/89 (1%) Query: 417 GDWVAVGNADFGSGASSFKANVASATGGGKIEIRLDSATGPLVGTLNVPNTGGAQSWQEV 476 G W+A + + + A+ +EIR D GPLV +P TGG Q+W V Sbjct: 837 GSWIAFHDINLEQPTNLLTVRAANENKQAVLEIRADRVDGPLVSQSVIPPTGGLQAWTTV 896 Query: 477 QTTVSNATG-VHRLYLVFTGSGSGNLFNF 504 V+ T + LYLVF G + F+F Sbjct: 897 TAQVNGETSEIRDLYLVFKGEFQLSWFSF 925 >BH3231 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 51.2 bits (121), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 8/90 (8%), Positives = 18/90 (20%), Gaps = 4/90 (4%) Query: 417 GDWVAVGNADFGSGASSFKANVASATGGGKIEIRLDSATGPLVGTLNVPNTGGAQSWQEV 476 + +A + G + G + Sbjct: 210 LLNRSFEVGLAEVSQYEGVELLA-YSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGE 268 Query: 477 QTTVSNAT--GVHRLYLVFTGSGSGNLFNF 504 QT + A + R + + + F Sbjct: 269 QTQKAVAAYVDIARRHGL-DPAQMALAFVR 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 49,563,458 Number of extensions: 2146737 Number of successful extensions: 5087 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 5088 Number of HSP's successfully gapped: 2 Length of query: 641 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 528 Effective length of database: 25,345,689 Effective search space: 13382523792 Effective search space used: 13382523792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 119 (50.4 bits)