BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0268.0003.p (243 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2183 [M] COG1209 dTDP-glucose pyrophosphorylase 204 9e-53 MA3777 [M] COG1209 dTDP-glucose pyrophosphorylase 195 6e-50 STM2095 [M] COG1209 dTDP-glucose pyrophosphorylase 158 6e-39 rfbA [M] COG1209 dTDP-glucose pyrophosphorylase 158 7e-39 DRA0042 [M] COG1209 dTDP-glucose pyrophosphorylase 152 5e-37 >MA2183 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 241 Score = 204 bits (519), Expect = 9e-53, Method: Compositional matrix adjust. Identities = 107/241 (44%), Positives = 147/241 (60%), Gaps = 8/241 (3%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 +KGVILAGGTGSRLYPLT++ NKHLLP+ PM+ Y ++ L GI DI++V + AG Sbjct: 1 MKGVILAGGTGSRLYPLTKVTNKHLLPIYDKPMIYYPMETLINAGIKDIMIVSGRGHAGH 60 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 + + LGSG +FGV TY F+ G+ V+LGDN+F D+++ V Sbjct: 61 FLELLGSGVDFGVHFTYEIQEKAGGIAQALSLAEDFV-DGDSVTVILGDNIFQDNIKEDV 119 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + AK+ LK V DA R+GV + + IEEKP++PK+ + VTG+Y+YD Sbjct: 120 ANF----NSGAKIFLKEVTDAHRFGVAELKGKK---VIGIEEKPKNPKSDFAVTGLYIYD 172 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEAGEKLKN 240 VF+ I+ + PS RGELEITDVNN Y + + Y +L +WSDAGTF+SL AG +K Sbjct: 173 SDVFNAIKTLKPSGRGELEITDVNNYYINKGMMEYGILDGFWSDAGTFESLLRAGMLVKQ 232 Query: 241 T 241 Sbjct: 233 N 233 >MA3777 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 238 Score = 195 bits (495), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 110/242 (45%), Positives = 142/242 (58%), Gaps = 8/242 (3%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 +KGVILAGGTGSRLYPLT++ NKHLLPV PM+ Y I L GI +I++V + AG Sbjct: 1 MKGVILAGGTGSRLYPLTKVTNKHLLPVYDKPMIYYPIKTLIDAGIKEIMIVSGRGHAGH 60 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 + + LGSGA+FGV TY F + V+LGDN+F D+++ V Sbjct: 61 FLELLGSGADFGVKFTYEIQEEAGGIAQALSLAENFA-DEDDVTVILGDNIFQDNIKEDV 119 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + + AK+ LK V D R+GV + IEEKP PKT VTG+Y+YD Sbjct: 120 ENF----NNGAKIFLKEVPDVHRFGVAELKGDK---VIGIEEKPAVPKTNLAVTGLYIYD 172 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEAGEKLKN 240 VFD I+ + PS RGELEITDVNN Y + + Y VL+ +WSDAGTF+SL A +K Sbjct: 173 YDVFDAIKTLKPSGRGELEITDVNNYYVNKGVMEYRVLEGFWSDAGTFESLLRASMMVKG 232 Query: 241 TL 242 L Sbjct: 233 LL 234 >STM2095 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 292 Score = 158 bits (400), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 94/241 (39%), Positives = 133/241 (55%), Gaps = 17/241 (7%) Query: 2 KGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQY 61 KG+ILAGG+G+RLYP+T ++K LLP+ PM+ Y + L GI DIL++ + Q ++ Sbjct: 5 KGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTLMLAGIRDILIISTPQDTPRF 64 Query: 62 TDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFV------VLLGDNLFMDD 115 LG G+++G++L Y FI+ GE F+ ++LGDN+F Sbjct: 65 QQLLGDGSQWGLNLQY------KVQPSPDGLAQAFII-GEEFIGHDDCALVLGDNIFYGH 117 Query: 116 LEPYVKKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTG 175 P + + A V V D RYGV FD + T + +EEKP PK+ Y VTG Sbjct: 118 DLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDQKGTAV--SLEEKPLQPKSNYAVTG 175 Query: 176 IYMYDEAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKE--WWSDAGTFQSLYE 233 +Y YD +V ++ + + PS RGELEITD+N IY RLS ++ W D GT QSL E Sbjct: 176 LYFYDNSVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMMGRGYAWLDTGTHQSLIE 235 Query: 234 A 234 A Sbjct: 236 A 236 >rfbA [M] COG1209 dTDP-glucose pyrophosphorylase Length = 293 Score = 158 bits (399), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 92/235 (39%), Positives = 127/235 (54%), Gaps = 5/235 (2%) Query: 2 KGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQY 61 KG+ILAGG+G+RLYP+T ++K LLP+ PM+ Y + L GI DIL++ + Q ++ Sbjct: 5 KGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTLMLAGIRDILIISTPQDTPRF 64 Query: 62 TDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYVK 121 LG G+++G++L Y FI G+ ++LGDN+F P + Sbjct: 65 QQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFI-GGDDCALVLGDNIFYGHDLPKLM 123 Query: 122 KYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYDE 181 + A V V D RYGV FD T + +EEKP PK+ Y VTG+Y YD Sbjct: 124 EAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAI--SLEEKPLEPKSNYAVTGLYFYDN 181 Query: 182 AVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKE--WWSDAGTFQSLYEA 234 V + + + PS RGELEITD+N IY RLS ++ W D GT QSL EA Sbjct: 182 DVVQMAKNLKPSARGELEITDINRIYLEQGRLSVAMMGRGYAWLDTGTHQSLIEA 236 >DRA0042 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 296 Score = 152 bits (384), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 89/236 (37%), Positives = 132/236 (55%), Gaps = 7/236 (2%) Query: 2 KGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQY 61 KG+ILAGG+G+RLYP T ++K LLP+ PM+ Y + L GG+ +IL++ + + ++ Sbjct: 6 KGIILAGGSGTRLYPATLAVSKQLLPIYDKPMIYYPLTTLMLGGMREILIISTPEDTPRF 65 Query: 62 TDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFM-DDLEPYV 120 LG G+++G++L Y F+ G ++LGDN+F +DL + Sbjct: 66 KQLLGDGSQWGIALEYAVQPKPEGLAQAFLIGEDFV-QGHPSSLILGDNIFYGNDLSDLM 124 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + + G A V V D RYGV FD +G IEEKP PK+ + VTG+Y YD Sbjct: 125 QAANAKENG-ATVFAYQVRDPERYGVVDFDR--SGRALSIEEKPAQPKSDFAVTGLYFYD 181 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKE--WWSDAGTFQSLYEA 234 E V +I R + PS RGELEITD+NN+Y L +++ W D GT +S+ +A Sbjct: 182 ERVSEIARSIKPSPRGELEITDLNNVYLEEGALDVQLMRRGFAWLDTGTHESMLDA 237 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.141 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,099,047 Number of extensions: 736387 Number of successful extensions: 2126 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 2062 Number of HSP's successfully gapped: 55 Length of query: 243 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 140 Effective length of database: 26,499,029 Effective search space: 3709864060 Effective search space used: 3709864060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)