BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0267.0018.p (427 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3818 [R] COG1078 HD superfamily phosphohydrolases 452 e-127 BS_ywfO [R] COG1078 HD superfamily phosphohydrolases 433 e-121 SSO3125 [R] COG1078 HD superfamily phosphohydrolases 150 3e-36 MTH1481 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 138 2e-32 MTH1489 [G] COG1082 Sugar phosphate isomerases/epimerases 138 2e-32 >BH3818 [R] COG1078 HD superfamily phosphohydrolases Length = 432 Score = 452 bits (1162), Expect = e-127, Method: Compositional matrix adjust. Identities = 222/408 (54%), Positives = 297/408 (72%), Gaps = 3/408 (0%) Query: 4 LLTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSL 63 LL EEKVFKDPVH YIHV+D +IW LI T+EFQRLRR+RQLGT++LTFHGAEH+RF+HSL Sbjct: 8 LLDEEKVFKDPVHRYIHVRDELIWALIGTKEFQRLRRVRQLGTTFLTFHGAEHTRFNHSL 67 Query: 64 GVYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTC 123 GVYEITRRII F+ Y W EE GHGPFSHS E+ F+ +HE+WT Sbjct: 68 GVYEITRRIIEVFQGRPY--WNEEERLLTLCAALLHDIGHGPFSHSFEKVFDTDHEEWTR 125 Query: 124 RIIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYT 183 R+I +TEI +L + G+DFPQKVA VI KTY +++V +++S +DADRMDYL RDAYYT Sbjct: 126 RMIVGDTEIHNVLLKMGDDFPQKVADVIEKTYPNKLVTSIISSQIDADRMDYLQRDAYYT 185 Query: 184 GVNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIIL 243 GV+YG D++RILR++RP +VV+K+SGMHA+EDY+MSRYQMYWQVYFHPVTRS+E+IL Sbjct: 186 GVSYGHFDMERILRVMRPMEDQVVIKQSGMHAVEDYIMSRYQMYWQVYFHPVTRSAEVIL 245 Query: 244 RQIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDDEIL 303 ++F+R K+L+++GY F+ LF G ++++ YL LDE++ F W E+D IL Sbjct: 246 SKVFKRVKDLYEQGYKFKQEPKHFYSLFEGNMSLDDYLRLDESITMYYFQIWQEEEDRIL 305 Query: 304 ANLCSRFIHRKLYKYVEMD-HLDSETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVFRP 362 ++LC RFI+R+L+KY+E + +L +++ FA A + P+Y L +D +DLPYD +RP Sbjct: 306 SDLCVRFINRQLFKYIEFNPNLQMNDWPRLQQLFAQAEIDPEYYLVVDSSSDLPYDFYRP 365 Query: 363 GEGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQDKL 410 GE + I L+ + KLRE+S SD+V +ISG R + LYFP D L Sbjct: 366 GEEEERLPIHLIMPNGKLRELSRESDVVEAISGKKRTDHKLYFPMDCL 413 >BS_ywfO [R] COG1078 HD superfamily phosphohydrolases Length = 433 Score = 433 bits (1114), Expect = e-121, Method: Compositional matrix adjust. Identities = 216/405 (53%), Positives = 288/405 (71%), Gaps = 2/405 (0%) Query: 5 LTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSLG 64 L+EEKVFKDPVH Y+HV+D +IW LI TREFQRLRRI+QLGT+YLTFHGAEHSRF+HSLG Sbjct: 8 LSEEKVFKDPVHRYVHVRDKLIWDLIGTREFQRLRRIKQLGTTYLTFHGAEHSRFNHSLG 67 Query: 65 VYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTCR 124 VYEI RR++ + G +W E GHGPFSHS E+ F ++HED+T Sbjct: 68 VYEIVRRMVDDVFK-GRPEWDDSERELCLAAALLHDLGHGPFSHSFEKVFHLDHEDFTRG 126 Query: 125 IIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYTG 184 II +TE+ +LR+ FPQ VA VIAKTY+++ VV+L+S +DADRMDYL RDAYYTG Sbjct: 127 IILGDTEVNQVLRKVSPGFPQDVAEVIAKTYKNKQVVSLISSQIDADRMDYLQRDAYYTG 186 Query: 185 VNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIILR 244 V+YG D++RILR++RP ++V+KESGMHA+EDY+MSRYQMYWQVYFHPVTRS+E+IL Sbjct: 187 VSYGHFDMERILRVMRPREDQIVIKESGMHAVEDYIMSRYQMYWQVYFHPVTRSAEVILT 246 Query: 245 QIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDDEILA 304 +I RAK+L EGY F +F G++ +E Y+ LDE+++ F W E+D IL+ Sbjct: 247 KILHRAKQLHDEGYVFTHAPVHFYSIFEGKLTLEDYVKLDESIILYYFQAWEDEEDAILS 306 Query: 305 NLCSRFIHRKLYKYVEMD-HLDSETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVFRPG 363 +LC RFI+R+L++YVE + + + E+ F AG+ P Y L +D +DLPYD +RPG Sbjct: 307 DLCRRFINRQLFQYVEFNPNEEMSAYFELTSLFKEAGIDPSYYLVVDSSSDLPYDFYRPG 366 Query: 364 EGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQD 408 E + I LL ++ +++E+S S IV SISG R + LY+P+D Sbjct: 367 EEEERLPIHLLTQNGQIKELSRQSAIVESISGKRRTDHKLYYPKD 411 >SSO3125 [R] COG1078 HD superfamily phosphohydrolases Length = 413 Score = 150 bits (380), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 133/444 (29%), Positives = 204/444 (45%), Gaps = 76/444 (17%) Query: 3 QLLTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHS 62 Q+L K+ +DP+H YI V+D + ++I+T FQRLR I Q G +YL + G H+RF HS Sbjct: 2 QILFFMKIIRDPIHGYIEVED-FVLQIISTEIFQRLRHITQTGLAYLVYPGMRHTRFEHS 60 Query: 63 LGVY----EITRRIISQFERSGYKDWMPEESXXXXXXXXXXX-XGHGPFSHSIEEAFEMN 117 LGV E+TR I E+ D++ EE GH PFSH+ E A + Sbjct: 61 LGVMHLAKELTRYIKINSEQYTDLDFINEEYLKLVGLSGLLHDIGHLPFSHTFENALSLA 120 Query: 118 HEDW---------TCRIIQENTEITAILREAGE---------DFPQKVASVIAKTYEHEI 159 E + +I N I L + +F Q+V S +T E + Sbjct: 121 KEVYGIDVEYYGKKTHVIFGNRVIDYYLGNYLDKLSKNYDVVNFVQRVISSTPRTKEESL 180 Query: 160 VVNLVSGPLDADRMDYLLRDAYYTGVNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDY 219 ++S PLDADR DYLLRD+Y+ GV YG DI+RI R L NG++ V + + +E + Sbjct: 181 ASLIISSPLDADRGDYLLRDSYFAGVGYGNFDIERIKRSLIYVNGKLAVLKKAIPVVEQF 240 Query: 220 LMSRYQMYWQVYFHPVTRSSEIILRQIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQ 279 L++R MY +YFH V +L + L Q+ + P + E Sbjct: 241 LLARMYMYETIYFHSVVGLYNAVLSHAVVK---LIQKN------LIP-------NVAPEN 284 Query: 280 YLLLDEALVQTAFMQWTLEDDEILANLCSRFIHRKLYKYVEMDHLDS---------ETID 330 YL L++ ++ + + E ++RK +K + D + + I+ Sbjct: 285 YLKLNDVVIMSKLNEAGRE-------FFDAIVYRKGFKRYKKDLTGNCYETLEKKRKEIN 337 Query: 331 EIRRSFAGAGLHPDYDLEIDYPTDLPYDVFRPGEGFDSKQILLLDRHDKLREISEVSDIV 390 EI R G L+ D+ D+PY +I + D D+L+ +S S IV Sbjct: 338 EIMRENNGLILYHDF-------YDVPY---------SEDEIFVYD-DDELKPLSTESKIV 380 Query: 391 RSISGIHRG--RYHLYFPQDKLQK 412 S++ I +G YH +DK++K Sbjct: 381 SSLTIIKKGIVGYH-ESAEDKMKK 403 >MTH1481 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 211 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 0/242 (0%), Positives = 0/242 (0%), Gaps = 18/242 (7%) Query: 9 KVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSLGVYEI 68 K ++ + + S + + + V Sbjct: 2 NKAKRLEILTRLRENNPH-PTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 69 TRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTCRIIQE 128 ++ + + E + + Sbjct: 61 PAAMLELG------VEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEAL 114 Query: 129 NTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYTGVNYG 188 + + V + + Sbjct: 115 PGVGRKT----ANVVLNTAFGWPTIA--VDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVP 168 Query: 189 TIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIILRQIFR 248 L + Sbjct: 169 AEFKVDCHHWLILHGR----- 223 Query: 249 RA 250 Sbjct: 224 225 >MTH1489 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 268 Score = 138 bits (347), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 12/242 (4%), Positives = 35/242 (14%), Gaps = 18/242 (7%) Query: 9 KVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSLGVYEI 68 + + L L + + +I Sbjct: 2 NRVDLSIFIPDSLTAETG-DLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDI 60 Query: 69 TRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTCRIIQE 128 + + + H + + Sbjct: 61 LTYMDTPQ------YLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRV 114 Query: 129 NTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYTGVNYG 188 + + I+ V ++ + Y G Sbjct: 115 KKGTLVD----IGADKLALCREKLTV--NRIMSFRVVRLGKEILIEPDEPEDRYWGYEVL 168 Query: 189 TIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIILRQIFR 248 + + VVV S + ++ + + Sbjct: 169 DTRRNLAESLKTVGAD-VVVATSRNASPITSILDEVKTRMRGAREAAIL----FGGPYKG 223 Query: 249 RA 250 Sbjct: 224 LP 225 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,774,917 Number of extensions: 1339044 Number of successful extensions: 2066 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 2032 Number of HSP's successfully gapped: 18 Length of query: 427 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 318 Effective length of database: 25,807,025 Effective search space: 8206633950 Effective search space used: 8206633950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 117 (49.7 bits)