BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0267.0015.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMB2088 [R] COG0313 Predicted methyltransferases 174 2e-43 NMA0342 [R] COG0313 Predicted methyltransferases 171 2e-42 RSc3266 [R] COG0313 Predicted methyltransferases 137 2e-32 NMB1908 [R] COG0313 Predicted methyltransferases 103 2e-22 NMA0547 [R] COG0313 Predicted methyltransferases 100 4e-21 >NMB2088 [R] COG0313 Predicted methyltransferases Length = 291 Score = 174 bits (440), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 94/220 (42%), Positives = 136/220 (61%), Gaps = 3/220 (1%) Query: 21 GSLYLVATPIGNLEDMTYRAVRTLRECDIIAAEDTRQTRKLLSHFEITSSMLFSYHEHNK 80 G+LY+VATPIGNL D+T RA+ L++ DII AEDTR T +LLS + I L S EHN+ Sbjct: 16 GTLYVVATPIGNLADITLRALAVLQKADIICAEDTRVTAQLLSAYGIQGK-LVSVREHNE 74 Query: 81 AASGPELIRYIIEGKNLALVSDAGLPAISDPGADLVALAVSHGIPVIPIPGANAALSALI 140 +++ Y+ +G +A VSDAG PA+ DPGA L G V+P+ GA+A ++AL Sbjct: 75 RQMADKIVGYLSDGMVVAQVSDAGTPAVCDPGAKLARRVREAGFKVVPVVGASAVMAALS 134 Query: 141 ASGLPTTSFTFIGFLPRERKDIRSVLSGLRSAQGTLLFYESPHRVSKTLAHLQEAFGNRR 200 +G+ + F F GF+P + + R + + A ++ +E+PHR+ TLA + E F RR Sbjct: 135 VAGVEGSDFYFNGFVPPKSGERRKLFAKWVRAAFPIVMFETPHRIGATLADMAELFPERR 194 Query: 201 IVLARELTKRYEEFLRGTIEECLDWLSE--HPPLGEYVIV 238 ++LARE+TK +E FL GT+ E LS + GE V+V Sbjct: 195 LMLAREITKTFETFLSGTVGEIQTALSADGNQSRGEMVLV 234 >NMA0342 [R] COG0313 Predicted methyltransferases Length = 171 Score = 171 bits (432), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 0/220 (0%), Positives = 0/220 (0%), Gaps = 3/220 (1%) Query: 21 GSLYLVATPIGNLEDMTYRAVRTLRECDIIAAEDTRQTRKLLSHFEITSSMLFSYHEHNK 80 L ++ + + ++ A + A + L + + Sbjct: 16 TRLSILESLSEGDKHISGLAREIGISVPVAAKHVKVLEKAKLIERKKFGN-THMIGIKLN 74 Query: 81 AASGPELIRYIIEGKNLALVSDAGLPAISDPGADLVALAVSHGIPVIPIPGANAALSALI 140 + + S ++ + + Sbjct: 75 NVYSFLDRFAENRKLEVEEGTSLLEALKSVAAVEVKKMGDRIKVVSTDGEEGFYIYEVDG 134 Query: 141 ASGLPTTSFTFIGFLPRERKDIRSVLSGLRSAQGTLLFYESPHRVSKTLAHLQEAFGNRR 200 Sbjct: 135 194 Query: 201 IVLARELTKRYEEFLRGTIEECLDWLSE--HPPLGEYVIV 238 Sbjct: 195 234 >RSc3266 [R] COG0313 Predicted methyltransferases Length = 300 Score = 137 bits (345), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/205 (39%), Positives = 123/205 (60%), Gaps = 5/205 (2%) Query: 15 NQPEATGSLYLVATPIGNLEDMTYRAVRTLRECDIIAAEDTRQTRKLLSHFEITSSMLFS 74 +Q G+LY+VATPIGN D++ RA L D +A EDTR T +LL ++ +L + Sbjct: 12 DQHYPAGALYVVATPIGNAADVSLRARHVLGLADAVACEDTRNTGQLLHRLGLSRPLL-A 70 Query: 75 YHEHNKAASGPELIRYIIEGKNLALVSDAGLPAISDPGADLVALAVSHGIPVIPIPGANA 134 HEHN+ + ++ + G+ +A VSDAG P +SDPGA +V + G VIP+PGA+A Sbjct: 71 AHEHNEREAAARIVERLARGERIAYVSDAGTPGVSDPGARIVEAVRAAGQRVIPLPGASA 130 Query: 135 ALSALIASG--LPTTS--FTFIGFLPRERKDIRSVLSGLRSAQGTLLFYESPHRVSKTLA 190 ++AL A+G L T++ FTF+GFLP + + + + + YE+PHR+ TLA Sbjct: 131 VVTALSAAGALLETSAGQFTFVGFLPPKAGQRDTAIRQWAAHGPAWVLYEAPHRIDATLA 190 Query: 191 HLQEAFGNRRIVLARELTKRYEEFL 215 L RR+++ RELTK +E+ + Sbjct: 191 ALAAHLPARRLLIGRELTKLFEQIV 215 >NMB1908 [R] COG0313 Predicted methyltransferases Length = 241 Score = 103 bits (258), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 74/211 (35%), Positives = 114/211 (54%), Gaps = 17/211 (8%) Query: 23 LYLVATPIGN-----LEDMTYRAVRTLRECDIIAAEDTRQTRKLLSHFEITSSM----LF 73 LYL+ TP+G L +A+ L + ++ AE T R L H +T+ + L Sbjct: 10 LYLIPTPLGTPDTPCLLPHEQQAIVGLTDF-VVEAEKT--ARAHLKHLGVTTPIRELNLQ 66 Query: 74 SYHEHNKAASGPELIRYIIEGKNLALVSDAGLPAISDPGADLVALAVSHGIPVIPIPGAN 133 + +EH + PEL++ + EG+++ +VS+AG PA++DPGA+LVALA HG V P+ G + Sbjct: 67 TLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGANLVALAHKHGFEVRPLVGPS 126 Query: 134 AALSALIASGLPTTSFTFIGFLPRERKD----IRSVLSGLRSAQGTLLFYESPHRVSKTL 189 + L AL+ASG SF F G+LP E+ + R++ R T +F E+P+R L Sbjct: 127 SLLLALMASGANGQSFAFKGYLPSEKNERIQAFRALEQRSRQCGETQIFIETPYRNDALL 186 Query: 190 AHLQEAF-GNRRIVLARELTKRYEEFLRGTI 219 A E R+ A +LT +E + TI Sbjct: 187 ADAVENLHPETRLCTATDLTLPTQEIISQTI 217 >NMA0547 [R] COG0313 Predicted methyltransferases Length = 237 Score = 100 bits (248), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 9/211 (4%), Positives = 35/211 (16%), Gaps = 17/211 (8%) Query: 23 LYLVATPIGN-----LEDMTYRAVRTLRECDIIAAEDTRQTRKLLSHFEITSSM----LF 73 + V + + E D+ ++ + Sbjct: 10 VPTVPDIEYLKKLALRGAVNKTVKVSSSEF-HKHTGDS--SKTAARKLKQMEEERLIERK 66 Query: 74 SYHEHNKAASGPELIRYIIEGKNLALVSDAGLPAISDPGADLVALAVSHGIPVIPIPGAN 133 + I + + P + + +++ + N Sbjct: 67 IVPGGQLIKMTEKGIEILKNEYIEYSRIFSSEPDVLELEGNVLKGLGEGQYYINIPGYRN 126 Query: 134 AALSALIASGLPTTSFTFIGFLPRERKDIRSVLSGL----RSAQGTLLFYESPHRVSKTL 189 L P T + ++ + + + + + + Sbjct: 127 QFEEKLNFVPFPGTLNVQLSESSSALRNRLKEMPAVRIDGFNDGERTFGGGNCYPIKVEG 186 Query: 190 AHLQEAF-GNRRIVLARELTKRYEEFLRGTI 219 + Sbjct: 187 IEAAVVVPDRTHYPADLIEIIAPVKLRDALK 217 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,665,960 Number of extensions: 724025 Number of successful extensions: 1992 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1998 Number of HSP's successfully gapped: 6 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)