BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0267.0011.p (328 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0686 [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases 114 2e-25 alr4932 [L] COG2812 DNA polymerase III, gamma/tau subunits 103 5e-22 all4705 [L] COG0470 ATPase involved in DNA replication 100 6e-21 BB0461 [L] COG2812 DNA polymerase III, gamma/tau subunits 91 2e-18 MG420 [L] COG2812 DNA polymerase III, gamma/tau subunits 90 6e-18 >STM0686 [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases Length = 252 Score = 114 bits (285), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 10/231 (4%), Positives = 35/231 (15%), Gaps = 11/231 (4%) Query: 6 SFHDILGQEDAKRLLQNALRKDAVSHAYLFTGPSGSGQMKTALMFAQAIFCTTCED-DAC 64 + + V+ G S + + ++ Sbjct: 12 FSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEA 71 Query: 65 GECLECRKVEHGNHPDLSLLKPDGASIK-IDQIRELQRMFSYRSEGVNPKVYIIEGADKM 123 L + + + + + + + + Sbjct: 72 RSALPDCQFVEADIRNW--QPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQ 129 Query: 124 TVQAANSLLKFLEEPPAPAVGILISDNSSSLLPTIQSRTQRIPFSPLHPEIMLQAL---- 179 L A + Sbjct: 130 MPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPS 189 Query: 180 SSEGVPV---PLARCAVSLTSGLDGCRELLAQNWFAEMRNLVLQLAKESLG 227 + R + + + L + E + + + + L Sbjct: 190 HQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 >alr4932 [L] COG2812 DNA polymerase III, gamma/tau subunits Length = 359 Score = 103 bits (256), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 13/180 (7%), Positives = 31/180 (17%), Gaps = 6/180 (3%) Query: 6 SFHDILGQEDAKRLLQNALRKDAVSHAYLFTGPSGSGQMKTALMFAQAIFCTTCED---D 62 + +E A A+ K L + Sbjct: 14 RHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITK 73 Query: 63 ACGECLECRKVEHGNHPDLSLLKPDGAS-IKIDQIRELQRMFSYRSEGVNPKVYIIEGAD 121 +S A + + + Sbjct: 74 PVLLLEGFFDARDLP--TISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLG 131 Query: 122 KMTVQAANSLLKFLEEPPAPAVGILISDNSSSLLPTIQSRTQRIPFSPLHPEIMLQALSS 181 QA + + ++S + + P + +++ E S Sbjct: 132 VRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSI 191 >all4705 [L] COG0470 ATPase involved in DNA replication Length = 673 Score = 99.8 bits (247), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 12/218 (5%), Positives = 25/218 (11%), Gaps = 26/218 (11%) Query: 7 FHDILGQEDAKRLLQNALRKDAVSHAYLFTGPSGSGQMKTALMFAQAIFCTTCEDDACGE 66 + L A + A+ T E Sbjct: 6 GQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKE 65 Query: 67 CLECRKVEHGNHPDLSLLKPD----------------------GASIKIDQIRELQRMFS 104 + + ++ + Sbjct: 66 RVG--QTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEG 123 Query: 105 YRSEGVNPKVYIIEGADKMTVQAANSLLKFLEEPPAPAVGILISDNSSSLLPTIQSRTQR 164 + +I I Sbjct: 124 LIEDDKVLLQQLISTISNWKNDLKTPSQAAASA-IGERDRIFAHCYGLYDAHLKACNVLD 182 Query: 165 IPFSPLHPEIMLQALSSEGVPVPLARCAV-SLTSGLDG 201 L P ++LQA + Sbjct: 183 FDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTN 220 >BB0461 [L] COG2812 DNA polymerase III, gamma/tau subunits Length = 560 Score = 91.3 bits (225), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 54/169 (31%), Positives = 90/169 (53%), Gaps = 10/169 (5%) Query: 7 FHDILGQEDAKRLLQNALRKDAVSHAYLFTGPSGSGQMKTALMFAQAIFCTTCEDDA--- 63 F+ + GQ+ L++++ K+ +++AY+F+GP G G+ +A FA+ C C + Sbjct: 16 FNSLEGQDFVVETLKHSIEKNKIANAYIFSGPRGVGKTSSARAFAR---CLNCRNGPTVM 72 Query: 64 -CGECLECRKVEHGNHPDLSLLKPDGAS-IKIDQIRELQRMFSYRSEGVNPKVYIIEGAD 121 CGEC C+ +E N L +++ DGAS + IR+++ + ++YII+ Sbjct: 73 PCGECSNCKSIE--NDSSLDVVEIDGASNTSVQDIRQIKEEIMFPPAISKYRIYIIDEVH 130 Query: 122 KMTVQAANSLLKFLEEPPAPAVGILISDNSSSLLPTIQSRTQRIPFSPL 170 ++ A N+LLK +EEPP V I + S L TI+SR Q F L Sbjct: 131 MLSNSAFNALLKTIEEPPNYIVFIFATTESHKLPETIKSRCQHFSFKLL 179 >MG420 [L] COG2812 DNA polymerase III, gamma/tau subunits Length = 260 Score = 89.7 bits (221), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 55/178 (30%), Positives = 92/178 (51%), Gaps = 4/178 (2%) Query: 5 MSFHDILGQEDAKRLLQNALRKDAVSHAYLFTGPSGSGQMKTALMFAQAIFCTTCED-DA 63 ++F LGQE +++L NA+ +D + + Y+F+G G+G+ A + A+AI C + D Sbjct: 12 INFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLNWDQIDV 71 Query: 64 CGECLECRKVEHGNHPDLSLLKPDGASIK-IDQIRELQRMFSYRSEGVNPKVYIIEGADK 122 C C C+ + + D +++ D AS I+ IREL KVYI++ A Sbjct: 72 CNSCDVCKSINTNSAID--IVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHM 129 Query: 123 MTVQAANSLLKFLEEPPAPAVGILISDNSSSLLPTIQSRTQRIPFSPLHPEIMLQALS 180 +T Q+ LLK LEE P + I + + + TI SR Q F + +++L+ L+ Sbjct: 130 LTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLN 187 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,245,776 Number of extensions: 909003 Number of successful extensions: 2409 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2394 Number of HSP's successfully gapped: 15 Length of query: 328 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 221 Effective length of database: 26,037,693 Effective search space: 5754330153 Effective search space used: 5754330153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)