BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0267.0008.p (213 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA4433 [F] COG0125 Thymidylate kinase 103 3e-22 AF0061 [F] COG0125 Thymidylate kinase 91 1e-18 MTH1765 [F] COG0125 Thymidylate kinase 75 6e-14 SSO1192 [F] COG0125 Thymidylate kinase 73 3e-13 SSO11920 [GER] COG0697 Permeases of the drug/metabolite transpor... 73 3e-13 >MA4433 [F] COG0125 Thymidylate kinase Length = 206 Score = 103 bits (256), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 68/208 (32%), Positives = 110/208 (52%), Gaps = 15/208 (7%) Query: 5 GFFITLEGGDGSGKTTVLGRVAAYLQNHSMPYLITREPG-GIEIAEKIRSIILDPAHTAM 63 G ITLEG DGSGK+TV ++ + + TREP G + + I + Sbjct: 3 GKLITLEGIDGSGKSTVAKKLQENSELRVFEPVFTREPTRGTLTGNAVENAI----QSDT 58 Query: 64 DARTEALLYAASRSQHLAEVVEPALKEGLTVLCDRFVDSSLVYQGYARG----LGIEEVR 119 D E L+ A ++HLA++V+PAL++G TV+ DR+ DS YQG +E VR Sbjct: 59 DQLAELFLFTADHAEHLAKLVKPALEDGKTVISDRYSDSRYAYQGITLKNRLDNPLEWVR 118 Query: 120 SINQFATGGLMPDLTFYLDVDPEVGLSRIAANQDREVNRLDLESMAFHQKVREGYRQVVE 179 +++ T ++PDLTF D++PE+ + R + + E + F + VRE + + Sbjct: 119 DLHRGWT--VIPDLTFLFDIEPEIAVKRCGKRGE----QTKFEKIEFLRGVRELFLGLAA 172 Query: 180 SDPQRIVVLDANRPIHMVEQDIVQTLKD 207 +P+R V++DA+ VE+ +VQ + D Sbjct: 173 EEPERFVIVDASGSPEDVEKAVVQKILD 200 >AF0061 [F] COG0125 Thymidylate kinase Length = 196 Score = 90.9 bits (224), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 60/201 (29%), Positives = 106/201 (52%), Gaps = 17/201 (8%) Query: 6 FFITLEGGDGSGKTTVLGRVAAYLQNHSMPYLITREPGGIEIAEKIRSIILDPAHTAMDA 65 I +EG DG+GKTT+ +A L+ + +EPG + +KI+S + + Sbjct: 1 MLIAVEGIDGAGKTTIAAYIAELLKEKGYKVKVLKEPGDSKFGKKIKS-----SEERLSP 55 Query: 66 RTEALLYAASRSQHLAEVVEPALKEGLTVLCDRFVDSSLVYQGYARGLGIEEVRSINQFA 125 E L+ R E + PAL+ G V+ DR+ S++ YQ ARG+ +R +N+ Sbjct: 56 EEELELFLKDREIDARENILPALQSGYAVVMDRYYFSNIAYQS-ARGIDARLIREMNEKI 114 Query: 126 TGGLMPDLTFYLDVDPEVGLSRIAANQDREVNRLD-LESMAFHQKVREGYRQVVESDPQR 184 PDLT LDV+PE+ L R+ R+ +L E + + +KVR+ + +E+ + Sbjct: 115 APK--PDLTILLDVEPEIALERV-----RKRGKLSPFEKLDYLRKVRKCF---LENADET 164 Query: 185 IVVLDANRPIHMVEQDIVQTL 205 VV+DA++P+ V++++ + + Sbjct: 165 TVVVDASKPLEEVKEEVRKVI 185 >MTH1765 [F] COG0125 Thymidylate kinase Length = 268 Score = 75.5 bits (184), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 8/201 (3%), Positives = 23/201 (11%), Gaps = 20/201 (9%) Query: 6 FFITLEGGDGSGKTTVLGRVAAYLQNHSMPYLITREPGGIEIAEKIRSIILDP-AHTAMD 64 T + G++ Sbjct: 1 MNRVDLSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDI 60 Query: 65 ARTEALLYAASRSQHLAEVVEPALKEGLTVLCDRFVDSSLVYQGYARGLGIEEVRSINQF 124 R + E L + + Sbjct: 61 LTYMDTPQYLRRKV-FPIMRELKHVGIL-PPLRTPHHPTGKPVTGE-----YRQGLTVKR 113 Query: 125 ATGGLMPDLTFYLDVDPEVGLSRIAANQDREVNRLDLESMAFHQKVREGYRQVVESDPQR 184 L + ++ N+ + R Sbjct: 114 VK---KGTLVDIGADKLALCREKLTVNR-------IMSFRVVRLGKEILIEPD--EPEDR 161 Query: 185 IVVLDANRPIHMVEQDIVQTL 205 + + + + Sbjct: 162 YWGYEVLDTRRNLAESLKTVG 182 >SSO1192 [F] COG0125 Thymidylate kinase Length = 213 Score = 73.2 bits (178), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 59/221 (26%), Positives = 100/221 (45%), Gaps = 30/221 (13%) Query: 5 GFFITLEGGDGSGKTTVLGRVAAYLQNHSMPYLITREPGGIEIAEKIRSIILDPAHTAM- 63 G I EG DGSGK++ + +++ YL +E I II + M Sbjct: 3 GLLIAFEGIDGSGKSSQAVLLKDWIEMRRDVYLTEWNS-----SEWIHDIIKEAKKKNML 57 Query: 64 DARTEALLYAASRSQHLAEVVEPALKEGLTVLCDRFVDSSLVYQGYA----RGLGIEEVR 119 + T +L++A S + P LK G V+CDR+ VY YA R + + V+ Sbjct: 58 TSITFSLIHATDFSDRYERYILPMLKSGFVVICDRY-----VYTAYARDVVRNVDFDWVK 112 Query: 120 SINQFATGGLMPDLTFYLDVDPEVGLSRIAANQDR---------EVNRLDLES--MAFHQ 168 + FA + P+ TFY+ V PE+ L RI + + + + LE + + Sbjct: 113 RLYSFA---IKPNFTFYIRVTPEIALERIRKAKRKIKPQEAGIDILGEIPLEEGFLKYQS 169 Query: 169 KVREGYRQVVESDPQRIVVLDANRPIHMVEQDIVQTLKDRI 209 ++ E Y ++ + + + +D NRP+ V+ DI + L + I Sbjct: 170 RIVEIYDKIAKEESN-FITIDGNRPLKDVQIDIRKILGEYI 209 >SSO11920 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 213 Score = 73.2 bits (178), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 59/221 (26%), Positives = 100/221 (45%), Gaps = 30/221 (13%) Query: 5 GFFITLEGGDGSGKTTVLGRVAAYLQNHSMPYLITREPGGIEIAEKIRSIILDPAHTAM- 63 G I EG DGSGK++ + +++ YL +E I II + M Sbjct: 3 GLLIAFEGIDGSGKSSQAVLLKDWIEMRRDVYLTEWNS-----SEWIHDIIKEAKKKNML 57 Query: 64 DARTEALLYAASRSQHLAEVVEPALKEGLTVLCDRFVDSSLVYQGYA----RGLGIEEVR 119 + T +L++A S + P LK G V+CDR+ VY YA R + + V+ Sbjct: 58 TSITFSLIHATDFSDRYERYILPMLKSGFVVICDRY-----VYTAYARDVVRNVDFDWVK 112 Query: 120 SINQFATGGLMPDLTFYLDVDPEVGLSRIAANQDR---------EVNRLDLES--MAFHQ 168 + FA + P+ TFY+ V PE+ L RI + + + + LE + + Sbjct: 113 RLYSFA---IKPNFTFYIRVTPEIALERIRKAKRKIKPQEAGIDILGEIPLEEGFLKYQS 169 Query: 169 KVREGYRQVVESDPQRIVVLDANRPIHMVEQDIVQTLKDRI 209 ++ E Y ++ + + + +D NRP+ V+ DI + L + I Sbjct: 170 RIVEIYDKIAKEESN-FITIDGNRPLKDVQIDIRKILGEYI 209 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,478,435 Number of extensions: 594708 Number of successful extensions: 12496 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 12482 Number of HSP's successfully gapped: 6 Length of query: 213 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 111 Effective length of database: 26,614,363 Effective search space: 2954194293 Effective search space used: 2954194293 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 113 (48.1 bits)