BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0266.0010.p (298 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3600 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 177 2e-44 STM1521 [U] COG2095 Multiple antibiotic transporter 169 4e-42 STM2489 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 166 5e-41 RSc1145 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 165 7e-41 ECs3340 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 164 2e-40 >CAC3600 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 286 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 93/287 (32%), Positives = 164/287 (57%), Gaps = 5/287 (1%) Query: 6 QIYGIYVPVVTPFNAAGEIDLESYQRYVNNIIKNSIHGLVVNGTTGESPTVNIQELQTLV 65 +I G+ +P++TPF G++DL SY++ + + K + G + TTGE+PT++ E Q+++ Sbjct: 2 KIEGVLIPLITPF-KDGKVDLASYEKLIRHYSKKGVAGFMPLATTGETPTLSDYEYQSIL 60 Query: 66 NTSREILKSSSIPLVIGTGTNDTASTVARTELAANSGADAVLVVVPYYSRPSQQGIIEHF 125 + E ++P+ +G G N+T ++ +L V PYY+RP Q+GI EHF Sbjct: 61 EKTVEC-NELNLPIYVGFGGNNTEKMTKDIKMLDKYNIKGILSVCPYYNRPDQRGIYEHF 119 Query: 126 RKAAE-VGIPVIAYDIPGRTGVGMSVDTARTILDMNNVVGLKDCSGSPLLVTEL--SRLG 182 ++ +E + ++ Y+IP RTG + DT R + +++N+VG+KD G TEL +R Sbjct: 120 KRISESTSLDIVLYNIPYRTGRNIENDTIRRLSELDNIVGVKDACGDFTQTTELLLNRPD 179 Query: 183 SKPVLCGDDLHFFDXXXXXXXXXXXXXXNVHTAKFLGIYEQFRAGQIEEARAAFDRLIPL 242 + +L G+D F+ +++T K++ ++ + + + A + ++ + Sbjct: 180 NFSILTGEDAFFYSTLMLGGDGGIMASAHLNTEKYVEVFNKSKQNDYKSALEIWKQVANM 239 Query: 243 MKLLFKESNPAPIKWLLSAYGEIASDTLRLPMTSISSGLREELGAYL 289 + LLF+E NPAPIK+ LS G IAS+ LRLP+ IS L+E+L +L Sbjct: 240 IPLLFEEPNPAPIKYCLSKTGVIASEALRLPLVPISENLKEKLNKFL 286 >STM1521 [U] COG2095 Multiple antibiotic transporter Length = 221 Score = 169 bits (428), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 0/294 (0%), Positives = 0/294 (0%), Gaps = 10/294 (3%) Query: 9 GIYVPVVTPFNAAGEIDLESYQRYVNNIIKNSIHGLVVNGTTGESPTVNIQELQTLVNTS 68 + + + + +++ N + + + Sbjct: 4 LFKAIGLGLVVL-LPLANPLTTVALFLGLAGNMNSAERNRQSYMASVYVFAIMMVAYYAG 62 Query: 69 REILKSSSIPLVIGTGTNDTASTVARTELAANSGADAVLVVVPYYSRPSQQGIIEHFRKA 128 + ++ ++ + G A Sbjct: 63 QLVM-NTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAHESPEAKSKSEELADEPTANIAF 121 Query: 129 AE-VGIPVIAYDIPGRTGVGMSVDT-ARTILDMNNVVGLKDCSGSPLLVTELSRLGSKP- 185 S D +V + ++ S Sbjct: 122 VPLAMPSTAGPGTIAMIISSASTVRHGGEFPDWVIMVAPPIIFLAVAVILWGCLRSSGAI 181 Query: 186 ----VLCGDDLHFFDXXXXXXXXXXXXXXNVHTAKFLGIYEQFRAGQIEEARAAFDRLIP 241 Sbjct: 182 MRLV 241 Query: 242 LMKLLFKESNPAPIKWLLSAYGEIASDTLRLPMTSISSGLREELGAYLAGSNHL 295 Sbjct: 242 - 294 >STM2489 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 211 Score = 166 bits (419), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 0/284 (0%), Positives = 0/284 (0%), Gaps = 4/284 (1%) Query: 9 GIYVPVVTPFNAAGEIDLESYQRYVNNIIKNSIHGLVVNGTTGESPTVNIQELQTLVNTS 68 + ++T + ++ + + Sbjct: 4 AKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAA 63 Query: 69 REILKSSSIPLVIGTGTNDTASTVARTELAANSGADAVLVVVPYYSRPSQQGIIEHFRKA 128 S + VP + + A Sbjct: 64 ML-ELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTA 122 Query: 129 AE-VGIPVIAYDIPGRTGVGMSVDTARTILDMNNVVGLKDCSGSPLLVTELSRLGSKP-- 185 + I T + + + N + ++ Sbjct: 123 NVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILH 182 Query: 186 VLCGDDLHFFDXXXXXXXXXXXXXXNVHTAKFLGIYEQFRAGQIEEARAAFDRLIPLMKL 245 Sbjct: 183 242 Query: 246 LFKESNPAPIKWLLSAYGEIASDTLRLPMTSISSGLREELGAYL 289 Sbjct: 243 286 >RSc1145 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 294 Score = 165 bits (418), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 104/287 (36%), Positives = 156/287 (54%), Gaps = 5/287 (1%) Query: 6 QIYGIYVPVVTPFNAAGEIDLESYQRYVNNIIKNSIHGLVVNGTTGESPTVNIQELQTLV 65 QI G V +VTP + G +D S + ++ I G+V+ GT+GESPTV++ E + L+ Sbjct: 3 QISGSLVAIVTPMHEDGSLDFPSLRSLIDWHIAEGTDGIVIVGTSGESPTVSVDEHRELI 62 Query: 66 NTSREILKSSSIPLVIGTGTNDTASTVARTELAANSGADAVLVVVPYYSRPSQQGIIEHF 125 + E + + IP++ GTG N T V T A + GADA L VVPYY++P+Q+G+ HF Sbjct: 63 RVTVEQV-NKRIPVIAGTGGNSTTEAVELTAYAKSVGADASLQVVPYYNKPTQEGMYLHF 121 Query: 126 RKAAE-VGIPVIAYDIPGRTGVGMSVDTARTILDMNNVVGLKDCSGSPLLVTELSRL--G 182 RK AE V +PVI Y++PGRT MSVDT + + V+G+K+ +G+ +L + Sbjct: 122 RKVAESVDLPVILYNVPGRTVADMSVDTMLRLAQVPGVMGVKEATGNIDRAAQLIKAAPA 181 Query: 183 SKPVLCGDDLHFFDXXXXXXXXXXXXXXNVHTAKFLGIYEQFRAGQIEEARAAFDRLIPL 242 S + GDD NV + G +E AR +L+ + Sbjct: 182 SFKIYSGDDPTAVALMLLGGHGNISVTANVAPRAMHDLCAAAMRGDVETARRIHMQLLSV 241 Query: 243 MKLLFKESNPAPIKWLLSAYGEIASDTLRLPMTSISSGLREELGAYL 289 K LF ESNP P+KW L A G++A +RLP+T +++ +E + A L Sbjct: 242 HKNLFVESNPIPVKWALQAMGKMAGG-IRLPLTPLAAQYQEVVRASL 287 >ECs3340 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 292 Score = 164 bits (414), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 98/284 (34%), Positives = 146/284 (51%), Gaps = 4/284 (1%) Query: 9 GIYVPVVTPFNAAGEIDLESYQRYVNNIIKNSIHGLVVNGTTGESPTVNIQELQTLVNTS 68 G V +VTP + G + S ++ ++ + + +V GTTGES T+N E +V + Sbjct: 4 GSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGTTGESATLNHDEHADVVMMT 63 Query: 69 REILKSSSIPLVIGTGTNDTASTVARTELAANSGADAVLVVVPYYSRPSQQGIIEHFRKA 128 E L IP++ GTG N TA ++ T+ +SG L V PYY+RPSQ+G+ +HF+ Sbjct: 64 LE-LADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLTVTPYYNRPSQEGLYQHFKAI 122 Query: 129 AE-VGIPVIAYDIPGRTGVGMSVDTARTILDMNNVVGLKDCSGSPLLVTELSRLGSKP-- 185 AE +P I Y++P RTG + +T + + N++G+K+ +G+ V ++ L S Sbjct: 123 AEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGIKEATGNLTRVNQIKELVSDDFV 182 Query: 186 VLCGDDLHFFDXXXXXXXXXXXXXXNVHTAKFLGIYEQFRAGQIEEARAAFDRLIPLMKL 245 +L GDD D NV + + G EAR RL+PL Sbjct: 183 LLSGDDASALDFMQLGGHGVISVTANVAARDMAQMCKLAAEGHFAEARVINQRLMPLHNK 242 Query: 246 LFKESNPAPIKWLLSAYGEIASDTLRLPMTSISSGLREELGAYL 289 LF E NP P+KW G +A+DTLRLPMT I+ RE + A L Sbjct: 243 LFVEPNPIPVKWACKELGLVATDTLRLPMTPITDSGRETVRAAL 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,100,888 Number of extensions: 873748 Number of successful extensions: 3384 Number of sequences better than 1.0e-05: 70 Number of HSP's gapped: 3252 Number of HSP's successfully gapped: 70 Length of query: 298 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 192 Effective length of database: 26,153,027 Effective search space: 5021381184 Effective search space used: 5021381184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)