BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0266.0008.p (234 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1386 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 99 5e-21 SSO1157 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 77 2e-14 SSO1567 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 63 4e-10 BH2193 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 59 7e-09 lin1904 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 55 9e-08 >CAC1386 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 243 Score = 99.4 bits (246), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 71/233 (30%), Positives = 110/233 (47%), Gaps = 14/233 (6%) Query: 7 MKIIE----LPIEFEYNGQPNYIYPSLIVSENELTLVDTGYPGFLPFIEKEIVAHGYDMM 62 MKI++ L +E G+ + I +L+ E + LVDTG+PG +I + + G + Sbjct: 1 MKIVDGVEMLELESNVMGKKSVINVTLLYDEENVVLVDTGFPGMSAYIREAVEKAGVALD 60 Query: 63 NLKNILITHYDDDHIGSLYAF-KAKYPSVTIMASGVEAEGISGKVKPERLAQAEAMLEHM 121 + I++TH D DHIGSL + K + + + E I G P +L + + Sbjct: 61 KVNKIILTHQDIDHIGSLASIIKESNKKIEVFSHEEEKPYIEGTKTPLKLVKFQD----- 115 Query: 122 PEEQQDFGHWFVQQLK---SLQHVSVDQTVHDGEWI-LNQQCKVIATPGHTSGHISLYFP 177 D ++LK + V+ T+ DGE + +VI TPGHT GHI LY Sbjct: 116 KANTSDQIRAICEKLKIGFEMAKSPVNTTLKDGEKLPFCGGIEVIQTPGHTFGHICLYIE 175 Query: 178 ELSSVITGDAAVRDGDELIIANPDFCLDRERADQSLNVLQNLQAKSYYCYHGG 230 + +I GDA L++A+P F D + +SL L ++ CYHGG Sbjct: 176 KSKLLIAGDALGAQDGVLMLADPLFDFDSNLSKKSLEKLTKYDIETVICYHGG 228 >SSO1157 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 213 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 65/230 (28%), Positives = 110/230 (47%), Gaps = 28/230 (12%) Query: 7 MKIIELPIEFEYNGQPNYIYPSLI-VSENELTLVDTGYPGFLPFIEKEIVAHGYDMMNLK 65 MK+ E+P+ F + LI +EN L L+D+G PG I + I G + +K Sbjct: 1 MKVFEIPLSF--------VKVFLIETNENGLILIDSGTPGSGRRIIEGITRLGKNPNKIK 52 Query: 66 NILITHYDDDHIGSLYAFKAKYPSVTIMASGVEAEGIS----GKVKPERLAQAEAMLEHM 121 +++ TH DHIG Y K V G++ G++ GK++ E + + A+ Sbjct: 53 SVIFTHSHVDHIGGAYEVKR---FVNDAKFGIDNNGVNYLKNGKIR-EPVLHSSAL---- 104 Query: 122 PEEQQDFGHWFVQQLKSLQHVSVDQTVHDGEWILNQQCKVIATPGHTSGHISLYFPELSS 181 + G F + V+VD + +GE + + ++I TPGHT+ IS+Y PEL++ Sbjct: 105 -KVIFSIGRPFF--FRKFNGVNVDFVLEEGELV--KGVEIIKTPGHTNDSISIYLPELNA 159 Query: 182 VITGDAAVRDGDELIIANPDFCLDRERADQSLNVLQNLQAKSYYCYHGGS 231 +I GD GD+ + P+ D + +S+ +++ Y HG S Sbjct: 160 IIVGDTL--QGDKRGLKYPNIYEDFDGLRKSVENIKSFNPSMVYVSHGVS 207 >SSO1567 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 233 Score = 62.8 bits (151), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 56/196 (28%), Positives = 92/196 (46%), Gaps = 13/196 (6%) Query: 36 LTLVDTGYPGFLPFIEKEIVAHGYDMMNLKNILITHYDDDHIGSLYAFKAKYPSVTIMAS 95 L ++DTG PG+L IE + A GY + ++ +I+ITH+ DH G+ A K + I A Sbjct: 42 LMMIDTGLPGYLDQIESYLKAWGYSLEDISDIVITHWHHDHAGNAMAIK-RISDAKIYAH 100 Query: 96 GVEAEGISGKVKPERLAQAEAMLEHMPEEQQDFGHWFVQQLKSLQH--VSVDQTVHDGEW 153 E + K + E + +P ++ ++++ L + V VD + GE Sbjct: 101 VDELGDLENPPKYSTIYSDELGVS-LPVFKRT-----MERINKLHYEPVKVDFALKGGED 154 Query: 154 ILNQQCKVIATPGHTSGHISLYFPELSSVITGDAAVRDGDELIIANPDFCLDRERADQSL 213 + +VI PGHT GHI+L+ + ++ GDA ++L F + E A S Sbjct: 155 L--GGFRVIHVPGHTKGHIALF--DGKCLVVGDAVRNVRNKLSPPLRIFSWNYELAVNSF 210 Query: 214 NVLQNLQAKSYYCYHG 229 N L +L +HG Sbjct: 211 NYLISLPYTVLIPFHG 226 >BH2193 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 259 Score = 58.9 bits (141), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 57/213 (26%), Positives = 88/213 (41%), Gaps = 29/213 (13%) Query: 34 NELTLVDTGYPGFLPFIEKEIVAHGYDMMNLKNILITHYDDDHIGSLYAFKAKYPSVTIM 93 ++ L+DTG FI K+I +++ K I++TH DH+G+L ++P+V + Sbjct: 26 SDWILIDTGLFYSEAFILKKIRRVLGELIPPKAIILTHGHFDHVGALRPLLQRFPNVPVY 85 Query: 94 ASGVEAEGISGK---VKPERLAQAEAMLEHM---PEEQQDFGHWFVQQLKSLQHVSVDQT 147 A +E ++G+ VKP+ M + P DF + Sbjct: 86 AHELELPFLTGQRHYVKPDPSVPGGLMAKLAWLYPYHAYDFSR------------IICPL 133 Query: 148 VHDGEWILNQQCKVIATPGHTSGHISLYFPELSSVITGDAAVRDGDELIIA--------- 198 DG + I TPGHT GH+SL+ P +I GDA E +++ Sbjct: 134 PDDGTLPFFTDWRWIHTPGHTEGHVSLFRPSDRFLIAGDAFTTVKQESLLSVLAQKKEIH 193 Query: 199 NPD--FCLDRERADQSLNVLQNLQAKSYYCYHG 229 P F D E A S+ LQ L+ HG Sbjct: 194 GPPAYFTPDWELAKTSVETLQKLEPAFAVTGHG 226 >lin1904 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 238 Score = 55.1 bits (131), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 56/213 (26%), Positives = 86/213 (40%), Gaps = 37/213 (17%) Query: 29 LIVSENELTLVDTGY----PGFLPFIEKEIVAHGYDMMNLKNILITHYDDDHIGSLYAFK 84 L++ ++ LTL+DTG G + I K + LK IL+TH DHIG L A K Sbjct: 25 LVLEKDGLTLIDTGILSHAKGIISLITKL-------KLPLKRILLTHGHGDHIGGLVALK 77 Query: 85 AKYPSVTIMASGVEAEGISGKVKPERLAQAEAMLEHMPEEQQDFGHWFVQQLKSLQHVSV 144 +P +M E + L + + + + E Q+ + Q V + Sbjct: 78 EAFPEALLMVGSRE----------KLLVETKEI--YAFEAQKPLKGSYPDQFP----VKI 121 Query: 145 DQTVHDGEWILNQQCKVIATPGHTSGHISLYFPELSSVITGD-------AAVRDGDELII 197 DQ + DG+ + ++ TPGHT G IS + + GD AA+ ++ Sbjct: 122 DQLLKDGDMV--GSLLIMDTPGHTPGSISFFDERNGHLFVGDLFQTRGGAAICGEKRILF 179 Query: 198 ANPDF-CLDRERADQSLNVLQNLQAKSYYCYHG 229 P D E + S LQ + C HG Sbjct: 180 PFPAMGSWDLETSIASAEKLQLVDVTEIACGHG 212 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,320,488 Number of extensions: 726352 Number of successful extensions: 3561 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 3564 Number of HSP's successfully gapped: 18 Length of query: 234 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 131 Effective length of database: 26,499,029 Effective search space: 3471372799 Effective search space used: 3471372799 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)