BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0266.0007.p (297 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PM1140 [M] COG0463 Glycosyltransferases involved in cell wall bi... 147 2e-35 CAC2173 [M] COG0463 Glycosyltransferases involved in cell wall b... 142 9e-34 HI1578 [M] COG0463 Glycosyltransferases involved in cell wall bi... 140 3e-33 NMA0524 [M] COG0463 Glycosyltransferases involved in cell wall b... 139 7e-33 NMB1929 [M] COG0463 Glycosyltransferases involved in cell wall b... 132 6e-31 >PM1140 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 337 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 81/230 (35%), Positives = 128/230 (55%), Gaps = 16/230 (6%) Query: 12 LMPVYNMAAYIEEAICSILTQTYSDFELLIIDDGSTDGTLDIIRKF--DDTRIKVIAHAV 69 L+ YN YIEE I +IL QTY + E+++++DGSTD TL + F D RIK+I + Sbjct: 12 LICAYNADKYIEECIDAILNQTYKNLEIVVVNDGSTDTTLSKLHYFAGKDPRIKIINNEE 71 Query: 70 NQGLIATLNQGIDCCRGEYIARMDGDDIALPHRFQRQVEFMDTHPDYGVCGSQVYLLGKD 129 N+G IA+LN GI G+Y+AR D DDI P ++ + +M +HP GS + +L +D Sbjct: 72 NKGFIASLNIGIASINGDYLARTDADDITKPEWIEKILGYMLSHPQIIAMGSYLTILSED 131 Query: 130 SVTT-------------KPVHHEEILCWQLLHCTIVHPTIMIRSSVLVNHGIRY-LDYPH 175 + P+ H EI+ L I + +++++S+V HG+R+ Y H Sbjct: 132 GNGSNLANYYEHGDEWRNPLSHREIVEAMLFRNPIHNNSMIVKSTVFREHGLRFDPAYQH 191 Query: 176 AEDYEIWNRLGAATKLMNLPEVLLMYRVHPGQISNTQQQVQLVQSERIRR 225 EDY+ W + +L N PE L+ YR+H Q S+ + Q + +++IR+ Sbjct: 192 TEDYQFWLEVSRLGELANYPESLVYYRLHNTQTSSLHNKYQNLMAKKIRK 241 >CAC2173 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 333 Score = 142 bits (357), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 94/297 (31%), Positives = 156/297 (52%), Gaps = 39/297 (13%) Query: 12 LMPVYNMAAYIEEAICSILTQTYSDFELLIIDDGSTDGTLDIIRKFD--DTRIKVIAHAV 69 +MPVYN Y++E+I SIL Q+YSD E +II+DGSTD + II+++ D RI VI+ Sbjct: 7 VMPVYNSEKYLKESIESILNQSYSDLEFIIINDGSTDNSFKIIKEYAKLDKRINVISRE- 65 Query: 70 NQGLIATLNQGIDCCRGEYIARMDGDDIALPHRFQRQVEFMDTHPDYGVCGSQVYLLGKD 129 N+G++ +LN+ I +GEYIARMD DDI+ P R ++Q+ F+ +H D + G+QV ++G Sbjct: 66 NKGIVYSLNEAIRLAKGEYIARMDADDISAPKRIEKQISFLKSHRDIDILGTQVKVVGNI 125 Query: 130 SVTTKPVHH--------------EEILCWQLLHCTIVHPTIMIRSSVLVN-HGIRYLDYP 174 S K + E+IL + +C + HP++M R +L G Y D+ Sbjct: 126 SNDIKEKNENKLNIEFDIYDDNREKILNY--WYC-LAHPSVMFRKDILRELKG--YNDFK 180 Query: 175 HAEDYEIWNR-LGAATKLMNLPEVLLMYRVHPGQISNTQQQVQLVQSERIRRDQLRLLGI 233 +ED ++W R + + K+ L E L+ +R+H + S T+ Q + +D +++ I Sbjct: 181 -SEDLDLWLRAIESGFKIYKLKEELIYFRMH--EESKTRVDNQNYEG---LKDGIKIKLI 234 Query: 234 EPTAEEYQTHLDFCHF---------RIRVHEPAHYYQCLAWAHKLLEGNLRTQRYHH 281 + E++ + + + + E QC+A+ K G R H Sbjct: 235 DVFKREFKKDFKYIVWGASNGGKITKEVLDEFFEKSQCIAFVDKFKTGEFEKIRILH 291 >HI1578 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 323 Score = 140 bits (353), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 80/231 (34%), Positives = 129/231 (55%), Gaps = 21/231 (9%) Query: 16 YNMAAYIEEAICSILTQTYSDFELLIIDDGSTDGTLDIIRKFD--DTRIKVIAHAVNQGL 73 YN YI+E+I SI+ QTY + E+++I+DGSTD TL + + D RIK+I++ N G Sbjct: 14 YNAEQYIDESISSIINQTYENLEIIVINDGSTDLTLSHLEEISKLDKRIKIISNKYNLGF 73 Query: 74 IATLNQGIDCCRGEYIARMDGDDIALPHRFQRQVEFMDTH---------------PDYGV 118 I +LN G+ C G+Y ARMD DDIA P ++ V +++ + + G+ Sbjct: 74 INSLNIGLGCFSGKYFARMDADDIAKPSWIEKIVTYLEKNDHITAMGSYLEIIVEKECGI 133 Query: 119 CGSQVYLLGKDSVTTKPVHHEEILCWQLLHCTIVHPTIMIRSSVLVNHGIRY-LDYPHAE 177 GSQ Y G + P+ H +I L + I + T+++R++V H + + DYP+AE Sbjct: 134 IGSQ-YKTG--DIWKNPLLHNDICEAMLFYNPIHNNTMIMRANVYREHKLIFNKDYPYAE 190 Query: 178 DYEIWNRLGAATKLMNLPEVLLMYRVHPGQISNTQQQVQLVQSERIRRDQL 228 DY+ W+ + L N PE L+ YR+H Q S+ Q +++I+R+ + Sbjct: 191 DYKFWSEVSRLGCLANYPEALVKYRLHGNQTSSVYNHEQNETAKKIKRENI 241 >NMA0524 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 110 Score = 139 bits (349), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 0/304 (0%), Positives = 0/304 (0%), Gaps = 40/304 (13%) Query: 12 LMPVYNMAAYIEEAICSILTQTYSDFELLIIDDGSTDGTLDIIRKFD--DTRIKVIAHAV 69 + ++ E + + + + Sbjct: 8 YVGSVYHCKECGYVGAFVVEANEEMIEKIKETYKREKRKEKKEEEEKEEEKDKEEGEEEE 67 Query: 70 NQGLIATLNQGID----CCRGEYIARMDGDDIALPHRFQRQVEFMDTHPDYGVCGSQVYL 125 + Sbjct: 68 KDKEEGEEEEKDKEEGE 127 Query: 126 LGKD-------------SVTTKPVHHEEILCWQLLHCTIVHPTIMIRSSVLVNHGIRY-L 171 Sbjct: 128 - 186 Query: 172 DYPHAEDYEIWNRLGAATKLMNLPEVLLMYRVHPGQISN--TQQQVQLVQSERIRRDQLR 229 Sbjct: 187 ---------- 236 Query: 230 LLGIEPTAEEYQTHLDFCHFRIRVHEPAHYYQCLAWAHKLLEGNLRTQRYHHETLNSVLS 289 Sbjct: 237 ------- 289 Query: 290 QCFS 293 Sbjct: 290 293 >NMB1929 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 349 Score = 132 bits (332), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 96/305 (31%), Positives = 154/305 (50%), Gaps = 41/305 (13%) Query: 12 LMPVYNMAAYIEEAICSILTQTYSDFELLIIDDGSTDGTLDIIRKF--DDTRIKVIAHAV 69 L+ YN+ Y +++ +++ QT+ + ++LI+DDGSTDGTL I ++F D RI+++A Sbjct: 24 LICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTDGTLAIAQRFQEQDGRIRILAQPR 83 Query: 70 NQGLIATLNQGID-----CCRGEYIARMDGDDIALPHRFQRQVEFMDTHPDYGVCGSQVY 124 N GLI +LN G+D GEYIAR D DDIA P ++ V M+ G+ + Sbjct: 84 NSGLIPSLNIGLDELAKSGGGGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLE 143 Query: 125 LLGKD-------------SVTTKPVHHEEILCWQLLHCTIVHPTIMIRSSVLVNHGIRY- 170 +L ++ + KP HE+I + I + T+++R SV ++ G+RY Sbjct: 144 VLSEEKDGNRLARHHEHGKIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSV-IDGGLRYN 202 Query: 171 LDYPHAEDYEIWNRLGAATKLMNLPEVLLMYRVHPGQISN--TQQQVQLVQSERIRRDQL 228 + AEDY+ W + +L PE L+ YR+H Q+S+ + +Q ++ Q Sbjct: 203 TERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQ--------- 253 Query: 229 RLLGIEPTAEEYQTHLDFCHFRIRVHEPAHYYQCLAWAHKLLEGNLRTQRYHHETLNSVL 288 GI+ TA L F+ R + Y Q A A++LLE +L + + E L Sbjct: 254 ---GIQKTAR--NDFLQSMGFKTRF-DSLEYRQIKAVAYELLEKHLPEEDF--ERARRFL 305 Query: 289 SQCFS 293 QCF Sbjct: 306 YQCFK 310 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.139 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,038,023 Number of extensions: 884391 Number of successful extensions: 2498 Number of sequences better than 1.0e-05: 109 Number of HSP's gapped: 2457 Number of HSP's successfully gapped: 110 Length of query: 297 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 191 Effective length of database: 26,153,027 Effective search space: 4995228157 Effective search space used: 4995228157 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)