BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0266.0005.p (626 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1596 [O] COG0326 Molecular chaperone, HSP90 family 402 e-111 YMR186w [O] COG0326 Molecular chaperone, HSP90 family 375 e-104 YPL240c [O] COG0326 Molecular chaperone, HSP90 family 368 e-101 PA1068 [O] COG0326 Molecular chaperone, HSP90 family 115 2e-25 >PA1596 [O] COG0326 Molecular chaperone, HSP90 family Length = 634 Score = 402 bits (1032), Expect = e-111, Method: Compositional matrix adjust. Identities = 234/634 (36%), Positives = 368/634 (58%), Gaps = 28/634 (4%) Query: 6 FKAESKRLLEMMINSIYTQREIFLRELISNASDAIDKIYYKALADEGLVFNKEDYYIKVA 65 F+ E K+LL +MI+S+Y+ +EIFLRELISNASDA DK+ ++ALA+ L+ + I+V+ Sbjct: 12 FQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAADKLRFEALANPELLEGGAELKIRVS 71 Query: 66 ADKASRTLTISDTGIGMTQEELENNLGIIANSGSFAFKKD---NEAKDGHNIIGQFGVGF 122 DK + T+T+ D GIGM++E++ +LG IA SG+ F K+ ++ KD H +IGQFGVGF Sbjct: 72 FDKEANTVTLEDNGIGMSREDVVTHLGTIAKSGTADFLKNLSGDQKKDSH-LIGQFGVGF 130 Query: 123 YSAFMVAEDVTVVSKALG--SDQAFKWESQGADGYTIEPTEKDTVGTEITLKIKANTEED 180 YSAF+VA+ V V S+ G + + W S+G + + +K GT I L +K EE Sbjct: 131 YSAFIVADKVDVYSRRAGQPASEGVHWSSKGEGEFDVATIDKPERGTRIVLHLKKGEEE- 189 Query: 181 NYDEFLEEYRLKSIIKKYSDFIRFPIKMEITSSKPKXXXXXXXXXXXXXQTVNSMVPIWR 240 F + +RL+++IKKYSD I PI++ K +TVN +W Sbjct: 190 ----FADGWRLRNVIKKYSDHIALPIEL----PKEFHGEEADKPAEPEWETVNRASALWT 241 Query: 241 KNKSELTDEDYNNFYTEKRYGFDKPLKHIHISADGAVVYNAILFIPENTPFDYYTKEYEK 300 + ++E+ DE+Y FY + F+ PL H +G + Y ++L++P PFD Y +E + Sbjct: 242 RPRAEVKDEEYQEFYKHVAHDFENPLSWSHNKVEGKLEYTSLLYVPGRAPFDLYHREAPR 301 Query: 301 GLELYSNGVLIMNKCADLLPDYFSFVKGMVDSEDLSLNISREMLQHDRQLTLIAKNIKSK 360 GL+LY V IM++ + LP Y F+KG+VDS DLSLN+SRE+LQ D + + + + Sbjct: 302 GLKLYVQRVFIMDQADEFLPLYLRFIKGVVDSNDLSLNVSREILQKDPVIDSMKSALTKR 361 Query: 361 IKSQLQSLLKDEREKYEKFYTSFGRQLKFGVYNDYGMEKETLQDLLMFYSSKE---KKQV 417 + L+ L K+E E+Y+ F+ +FG+ LK G D+G KE + LL F S+ + ++ V Sbjct: 362 VLDMLEKLAKNEPEQYKTFWKNFGQVLKEGPAEDFG-NKEKIAGLLRFASTGDDSGEQSV 420 Query: 418 TLAEYVERMPEDQKYIYYASGESVERIEKLPQTEMVSDKGYEILYFTDDIDEFAIKMIMS 477 LA+Y+ RM E Q IYY +GES +++ P E+ KG E+L TD IDE+ + + Sbjct: 421 ALADYIGRMKEGQDKIYYLTGESYSQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLPE 480 Query: 478 YKEKEFKNVSSGDL---GLEENAEDKPSXXXXXXXXXXXXXMKGILSGKVKDVKASKRLK 534 + K+F +V+ GDL L+ + K +K +L +V +V+ S RL Sbjct: 481 FDGKQFVDVARGDLDLGSLDSEEDKKAQEEVAKSKEGLIERLKKVLDEQVSEVRVSHRLT 540 Query: 535 SHPVCLST-EGELTIEMEKILKAMPNGQEVQADK-VLEINIHHDVFKSLKAAAEGDKEKL 592 P L+ E +L ++M +IL+A +GQ+V K + EIN H + + L AE D+++ Sbjct: 541 DSPAILAIGEQDLGLQMRQILEA--SGQKVPDSKPIFEINPQHPLIEKLD--AEPDEDRF 596 Query: 593 GLYTNLLYNQALLIEGLQVNDPVQFTNDICKIMV 626 G +++L++QA L G + DP + + K++V Sbjct: 597 GELSHILFDQAALAAGDSLKDPGAYVRRLNKLLV 630 >YMR186w [O] COG0326 Molecular chaperone, HSP90 family Length = 705 Score = 375 bits (964), Expect = e-104, Method: Compositional matrix adjust. Identities = 236/672 (35%), Positives = 370/672 (55%), Gaps = 61/672 (9%) Query: 5 EFKAESKRLLEMMINSIYTQREIFLRELISNASDAIDKIYYKALADEGLVFNKEDYYIKV 64 EF+AE +L+ ++IN++Y+ +EIFLRELISNASDA+DKI Y+AL+D + + D +I++ Sbjct: 7 EFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQALSDPKQLETEPDLFIRI 66 Query: 65 AADKASRTLTISDTGIGMTQEELENNLGIIANSGSFAFKKDNEAKDGHNIIGQFGVGFYS 124 + L I D+GIGMT+ EL NNLG IA SG+ AF + A ++IGQFGVGFYS Sbjct: 67 TPKPEEKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYS 126 Query: 125 AFMVAEDVTVVSKALGSDQAFKWESQGADGYTI---EPTEKDTVGTEITLKIKANTEEDN 181 F+VA+ V V+SK D+ + WES +T+ E E+ GT + L +K D+ Sbjct: 127 LFLVADRVQVISKN-NEDEQYIWESNAGGSFTVTLDEVNERIGRGTVLRLFLK-----DD 180 Query: 182 YDEFLEEYRLKSIIKKYSDFIRFPIKMEITSSKPKXX----------------------- 218 E+LEE R+K +IK++S+F+ +PI++ +T K Sbjct: 181 QLEYLEEKRIKEVIKRHSEFVAYPIQLLVTKEVEKEVPIPEEEKKDEEKKDEDDKKPKLE 240 Query: 219 ------------XXXXXXXXXXXQTVNSMVPIWRKNKSELTDEDYNNFYTEKRYGFDKPL 266 + +N P+W +N S++T E+YN FY ++ PL Sbjct: 241 EVDEEEEEKKPKTKKVKEEVQELEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPL 300 Query: 267 KHIHISADGAVVYNAILFIPENTPFDYY-TKEYEKGLELYSNGVLIMNKCADLLPDYFSF 325 H S +G + + AILFIP+ PFD + +K+ + ++LY V I ++ DL+P++ SF Sbjct: 301 YVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIPEWLSF 360 Query: 326 VKGMVDSEDLSLNISREMLQHDRQLTLIAKNIKSKIKSQLQSLLKDEREKYEKFYTSFGR 385 VKG+VDSEDL LN+SREMLQ ++ + +I KNI K+ + +D E+++KFY++F + Sbjct: 361 VKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAED-SEQFDKFYSAFAK 419 Query: 386 QLKFGVYNDYGMEKETLQDLLMFYSSKEKKQVT-LAEYVERMPEDQKYIYYASGESVERI 444 +K GV+ D + L LL + S+K ++T L +YV RMPE QK IYY +GES++ + Sbjct: 420 NIKLGVHED-TQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAV 478 Query: 445 EKLPQTEMVSDKGYEILYFTDDIDEFAIKMIMSYKEKEFKNVSSGDLGLEENAEDKPSXX 504 EK P + + K +E+L+ TD IDE+A + ++ K +++ D LEE E+K Sbjct: 479 EKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITK-DFELEETDEEKAERE 537 Query: 505 XXXXX-XXXXXXMKGILSGKVKDVKASKRLKSHPVCLSTEGEL--TIEMEKILKAMP--- 558 +K IL +V+ V S +L P + T G+ + ME+I+KA Sbjct: 538 KEIKEYEPLTKALKDILGDQVEKVVVSYKLLDAPAAIRT-GQFGWSANMERIMKAQALRD 596 Query: 559 --NGQEVQADKVLEINIHHDVFKSLKAAAE--GDKEK-LGLYTNLLYNQALLIEGLQVND 613 + + K EI+ + K LK + G ++K + TNLL+ ALL G + + Sbjct: 597 SSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTNLLFETALLTSGFSLEE 656 Query: 614 PVQFTNDICKIM 625 P F + I +++ Sbjct: 657 PTSFASRINRLI 668 >YPL240c [O] COG0326 Molecular chaperone, HSP90 family Length = 311 Score = 368 bits (944), Expect = e-101, Method: Compositional matrix adjust. Identities = 0/676 (0%), Positives = 0/676 (0%), Gaps = 65/676 (9%) Query: 5 EFKAESKRLLEMMINSIYTQREIFLRELISNASDAIDKIYYKALADEGLVFNKEDYYIKV 64 + ES + L+ L+ + +++ + Y+K+ Sbjct: 7 KHLLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYMKL 66 Query: 65 AADKASRTLTISDTGIGMTQEELENNLGIIANSGSFAFKKDNEAKDGHNIIGQFGVGFYS 124 + + + +K+ +G Sbjct: 67 DVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKALGNNW 126 Query: 125 AFMVAEDVTVVSKALGSDQAFKWESQGADGYTI---EPTEKDTVGTEITLKIKANTEEDN 181 + + + + + G + Sbjct: 127 NLFKGDANPLYDAY-IVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQ-----IA 180 Query: 182 YDEFLEEYRLKSIIKKYSDF----IRFPIKMEITSSKP---------------------- 215 ++ + F + Sbjct: 181 QGIRTWGTAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFFLEGAW 240 Query: 216 -------------KXXXXXXXXXXXXXQTVNSMVPIWRKNKSELTDEDYNNFYTEKRYGF 262 + K Sbjct: 241 VGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKLAMFAPKEFIPAIVGLMAVIGILIGSFG 300 Query: 263 DKPLKHIHISADGAVVYNAILFIPENTPFDYY-TKEYEKGLELYSNGVLIMNKCADLLPD 321 Sbjct: 301 360 Query: 322 YFSFVKGMVDSEDLSLNISREMLQHDRQLTLIAKNIKSKIKSQLQSLLKDEREKYEKFYT 381 Sbjct: 361 - 419 Query: 382 SFGRQLKFGVYNDYGMEKETLQDLLMFYSSKEKKQVT-LAEYVERMPEDQKYIYYASGES 440 Sbjct: 420 - 478 Query: 441 VERIEKLPQTEMVSDKGYEILYFTDDIDEFAIKMIMSYKEKEFKNVSSGDLGLEENAEDK 500 Sbjct: 479 - 537 Query: 501 PSXXXXXXX-XXXXXXMKGILSGKVKDVKASKRLKSHPVCLSTEGEL--TIEMEKILKAM 557 Sbjct: 538 - 596 Query: 558 P-----NGQEVQADKVLEINIHHDVFKSLKAAAE--GDKEK-LGLYTNLLYNQALLIEGL 609 Sbjct: 597 656 Query: 610 QVNDPVQFTNDICKIM 625 Sbjct: 657 672 >PA1068 [O] COG0326 Molecular chaperone, HSP90 family Length = 636 Score = 115 bits (288), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 92/371 (24%), Positives = 165/371 (44%), Gaps = 38/371 (10%) Query: 13 LLEMMINSIYTQREIFLRELISNASDAIDKIYYKALADEGLVFNKEDYYIKVAADKASRT 72 ++ ++ +Y+ + LREL+ NA D+I + + +G I+V D AS T Sbjct: 46 VMSVLSKHLYSTPTVALRELVQNAHDSILRRRLEQPDWQG------PSRIEVIGDSASNT 99 Query: 73 LTISDTGIGMTQEELENNLGIIANSGSFAFKKDNEAKDGHNIIGQFGVGFYSAFMVAEDV 132 + I DTG G+T+ E+ L + + ++ G +IG FG+GF SAF++A V Sbjct: 100 VRIVDTGAGLTEHEIHAYLATVGVGYTRGLRQSGHEDSG--LIGMFGLGFLSAFVLARRV 157 Query: 133 TVVSKALGSDQ-AFKWESQGADGYTIEPTEKDTVGTEITLKIKANTEEDNYDEFLEEYRL 191 +V + + + F + S A+ Y++ P VGTEITL++ +D Y + RL Sbjct: 158 SVRTTSYQQPELGFCYVSSNAEQYSVTPMPARPVGTEITLEL-----QDEYSALTQAARL 212 Query: 192 KSIIKKYSDFIRFPIKMEITSSKPKXXXXXXXXXXXXXQTVNSMVPIWRKNKSELT---D 248 + I+++Y +R PI + Q++N P WR Sbjct: 213 REILERYCVLLREPIHV-----------------GGDAQSINPEPPPWRLAADAALHPLQ 255 Query: 249 EDYNNFYTEKRYGFD-KPLKHIHISADGAVVYNAILFIPENTPFDYYTKEYEKGLELYSN 307 + R+ D +P+ + + D V +L+I + Y + L ++ Sbjct: 256 RQRRDLEFAARFEHDFEPICCLPVRPDERVDVQGLLWIQDG---GTYGTSDNRNLSVFLR 312 Query: 308 GVLIMNKCADLLPDYFSFVKGMVDSEDLSLNISREMLQHDRQLTLIAKNIKSKIKSQLQS 367 G+L+ + DLLP + FV G+++S L+ SRE LQ D I + + + L Sbjct: 313 GMLLDDDARDLLPSWAGFVGGVIESNRLTPTASREDLQRDDHYAAIQHALAEALIAGLGE 372 Query: 368 LLKDEREKYEK 378 + + + E + + Sbjct: 373 VARQQPEAWRR 383 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,338,841 Number of extensions: 1722759 Number of successful extensions: 3688 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3668 Number of HSP's successfully gapped: 6 Length of query: 626 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 514 Effective length of database: 25,461,023 Effective search space: 13086965822 Effective search space used: 13086965822 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 119 (50.4 bits)