BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0266.0004.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0745 [K] COG0583 Transcriptional regulator 208 7e-54 BS_yclA [K] COG0583 Transcriptional regulator 76 6e-14 BS_alsR [K] COG0583 Transcriptional regulator 72 1e-12 BS_ycgK [K] COG0583 Transcriptional regulator 70 3e-12 BH2117 [K] COG0583 Transcriptional regulator 68 2e-11 >CAC0745 [K] COG0583 Transcriptional regulator Length = 293 Score = 208 bits (530), Expect = 7e-54, Method: Compositional matrix adjust. Identities = 99/236 (41%), Positives = 148/236 (62%) Query: 61 RGPRSIQLTEAGMILRERAQQILELTDSTTRELKDFVKGVSGTLSIGTVSSSAATLLQDR 120 R R I+LT AG +L RA+Q+LEL D T +ELKDF +G+ GTLSIG +SS+ LL + Sbjct: 50 RTTRRIKLTNAGEMLLHRAKQMLELRDRTIKELKDFNEGLQGTLSIGAISSAGDILLPNW 109 Query: 121 LVEFHHNYSGVKFEIHEGNTYRIIDLLNKGIVEIGIVRTPFSSAGLECVYTVSEPMIAVM 180 + FH Y + F I E +T+ +++LLN G++E+GI+RTP +S E + EPMIA Sbjct: 110 IYNFHKKYPNIDFHIRECDTHEVLELLNNGLIEVGIIRTPLNSELYESILLPDEPMIAAA 169 Query: 181 APEYDWTGGKPEIEIGELRNQPLIVYRRFEQLIRETCLENGFDPQFFCMNDDARTTLLWA 240 W I++ +L ++PL+++RR+ ++I E C ++GF+P+ +D R LLWA Sbjct: 170 NENMFWRDEPKVIDMKDLSSKPLLMHRRYAKMIIEACEQSGFEPRVLGKIEDTRAILLWA 229 Query: 241 NAGLGIGIIPRSAFTLASNSNVAAKEIRSDSLNTRVAAVWMKDKYLSSLAAKFIEN 296 N G+G+ IIP+ + N N+ +I+ SL TR A VW K +YLS+ A F+EN Sbjct: 230 NTGMGVAIIPKDWIGIIPNINLKYAQIKKSSLKTRTAIVWKKGEYLSAAAKHFLEN 285 >BS_yclA [K] COG0583 Transcriptional regulator Length = 290 Score = 76.3 bits (186), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 55/240 (22%), Positives = 113/240 (47%), Gaps = 9/240 (3%) Query: 64 RSIQLTEAGMILRERAQQILELTDSTTRELKDFVKGVSGTLSIGTVSSSAATLLQDRLVE 123 + + LT G + +RA++IL + E+++ + V+GTL++G+ AA +L +++ + Sbjct: 54 KQMTLTYEGAVFLKRAKEILHRFEDAVIEVQELKEEVAGTLAVGSTIYCAALML-EKVTQ 112 Query: 124 FHHNYSGVKFEIHEGNTYRIIDLLNKGIVEIGIVRTPFSSAGLECVYTVSEPMIAVMAPE 183 Y + F I E +++LL ++ + T S ++ P + V++ E Sbjct: 113 IKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQLDDIPCVLVLSDE 172 Query: 184 YDWTGGKPEIEIGELRNQPLIVYRR------FEQLIRETCLENGFDPQFFCMNDDARTTL 237 + G I++ ++ + PLI+ R + Q++ E N +P+ C D+ T L Sbjct: 173 AGYPCGD-TIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLN-LEPRIVCECHDSATLL 230 Query: 238 LWANAGLGIGIIPRSAFTLASNSNVAAKEIRSDSLNTRVAAVWMKDKYLSSLAAKFIENF 297 ++G G I+P + + ++V I ++ A +W D YL A +F++ F Sbjct: 231 SLVSSGFGASILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF 290 >BS_alsR [K] COG0583 Transcriptional regulator Length = 302 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 63/244 (25%), Positives = 103/244 (42%), Gaps = 16/244 (6%) Query: 61 RGPRSIQLTEAGMILRERAQQILELTDSTTRELKDFVKGVSGTLSIGTVSSSAATLLQDR 120 R R ++LT AG I + L + +G G L IG V S+ L Sbjct: 50 RTKRFVELTAAGEIFLNHCRMALMQIGQGIELAQRTARGEQGLLVIGFVGSATYEFLPPI 109 Query: 121 LVEFHHNYSGVKFEIHEGNTYRIIDLLNKGIVEIGIVRTPFSSAGLECVYTVSEPMIAVM 180 + E+ + VK E+ E ++ R + L KG ++IGI+ P L S P + + Sbjct: 110 VREYRKKFPSVKIELREISSSRQQEELLKGNIDIGILHPPLQHTALHIETAQSSPCVLAL 169 Query: 181 APEYDWTGGKPEIEIGELRNQPLI---------VYRRFEQLIRETCLENGFDPQFFCMND 231 ++ T K I I +LR++P+I +Y F Q C + GF P Sbjct: 170 PKQHPLT-SKESITIEDLRDEPIITVAKEAWPTLYMDFIQF----CEQAGFRPNIVQEAT 224 Query: 232 DARTTLLWANAGLGIGIIPRSAFTLASNSNVAAKEIRSDSLNTRVAAVWMKDKYLSSLAA 291 + + + +AG+G+ +P SA L N +V +++ LN + KD + + L Sbjct: 225 EYQMVIGLVSAGIGMTFVPSSAKKLF-NLDVTYRKMDQIQLNAEWVIAYRKDNH-NPLIK 282 Query: 292 KFIE 295 FI Sbjct: 283 HFIH 286 >BS_ycgK [K] COG0583 Transcriptional regulator Length = 133 Score = 70.5 bits (171), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 0/251 (0%), Positives = 0/251 (0%), Gaps = 22/251 (8%) Query: 65 SIQLTEAGMILRERAQQILELTDSTTRELKDFVKGVSGTLSIGTVSSSAATLLQDRLVEF 124 + + + + + S L S A+ + V Sbjct: 54 FYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQ 113 Query: 125 HHNYSGVKFEIHEGNTYRIIDLLNKGIVEIGIVRTPFSSAGLECVYTVSEPMIAV----- 179 Sbjct: 114 173 Query: 180 ---MAPEY---------DWTGGKPEIEIGELRNQPLIVYRR---FEQLIRETCLENGFDP 224 Sbjct: 174 233 Query: 225 QFFCMNDDARTTLLWANAGLGIGIIPRSAFTLASNSNVAAKEIRSDSLNTRVAAVWMKDK 284 Sbjct: 234 -- 291 Query: 285 YLSSLAAKFIE 295 Sbjct: 292 302 >BH2117 [K] COG0583 Transcriptional regulator Length = 290 Score = 67.8 bits (164), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 55/212 (25%), Positives = 97/212 (45%), Gaps = 7/212 (3%) Query: 64 RSIQLTEAGMILRERAQQILELTDSTTRELKDFVKGVSGTLSIGTVSSSAATLLQDRLVE 123 + I LT+ G ++IL + +E+ +GT+ IGT +S+++ LL LV Sbjct: 54 KGISLTDIGQKTLPHIREILNRMEKINQEVALSTNLETGTIRIGTFASASSCLLPKLLVT 113 Query: 124 FHHNYSGVKFEIHEGNTYRIIDLLNKGIVEIGIVRTPFSSAGLECVYTVSEPMIAVMAPE 183 F + ++F +EG I + L GI++IG V S+ + + + M+ E Sbjct: 114 FEKKHPNIEFIFYEGTYEEISEWLQSGIIDIGFVVKGQSNPEFDVFPLIKDQMVVAFHSE 173 Query: 184 YDWTGGKPEIEIGELRNQPLIVYR-RFEQLIRETCLENGFDPQFFCMNDDARTTLLWANA 242 + + + +EI L+++P I+ ++ IRE E P D T Sbjct: 174 HSF-AQQTTVEIKALKDEPFIMPTGMYQSHIRELFEEGAIQPSVRFEVHDCATIANMVQE 232 Query: 243 GLGIGIIPRSAFTLASNSNVAAKEIRSDSLNT 274 GLG+ I P L++ SN+ EIR+ S+ + Sbjct: 233 GLGVTIGPE--LFLSTQSNI---EIRALSVTS 259 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,234,734 Number of extensions: 786307 Number of successful extensions: 3372 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 3367 Number of HSP's successfully gapped: 23 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)