BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0024.p (236 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1233 [R] COG2244 Membrane protein involved in the export of O-... 87 2e-17 BS_spoVB [R] COG2244 Membrane protein involved in the export of ... 80 2e-15 >BH1233 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 87.4 bits (215), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 7/123 (5%), Positives = 16/123 (13%) Query: 30 RRDLPTIHKRMHQALRLAMVTGAPFAAVMYIFAVPLCTLMYGNADTAPMLRMMAPFALFL 89 D+ P + + + Sbjct: 310 EIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKG 369 Query: 90 YVQAPLQAALQAMERPGRALVNTXXXXXXXXXXXXXXASQPEXXXXXXXXXXXXNSILVT 149 ++ A E N A + + L+ Sbjct: 370 LIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMN 429 Query: 150 MLH 152 Sbjct: 430 QQA 432 >BS_spoVB [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 80.5 bits (197), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 0/205 (0%), Positives = 0/205 (0%) Query: 30 RRDLPTIHKRMHQALRLAMVTGAPFAAVMYIFAVPLCTLMYGNADTAPMLRMMAPFALFL 89 D+ P + + + Sbjct: 310 EIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKG 369 Query: 90 YVQAPLQAALQAMERPGRALVNTXXXXXXXXXXXXXXASQPEXXXXXXXXXXXXNSILVT 149 ++ A E N A + + L+ Sbjct: 370 LIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMN 429 Query: 150 MLHGYXXXXXXXXXXXXADLLKTLAAMIIMAAGMSYIYTSVPIAPAQWAQFLFAAGSGMA 209 Sbjct: 430 489 Query: 210 LYFGICLLSGLISIRDLDRVPFIKK 234 Sbjct: 490 514 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.139 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,737,640 Number of extensions: 300760 Number of successful extensions: 3695 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 3695 Number of HSP's successfully gapped: 2 Length of query: 236 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 133 Effective length of database: 26,499,029 Effective search space: 3524370857 Effective search space used: 3524370857 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 114 (48.5 bits)