BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0023.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1233 [R] COG2244 Membrane protein involved in the export of O-... 197 2e-50 BS_spoVB [R] COG2244 Membrane protein involved in the export of ... 192 4e-49 CAC3213 [R] COG2244 Membrane protein involved in the export of O... 125 7e-29 CAC1016 [R] COG2244 Membrane protein involved in the export of O... 113 3e-25 BH3277 [R] COG2244 Membrane protein involved in the export of O-... 104 1e-22 >BH1233 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 14/272 (5%), Positives = 43/272 (15%), Gaps = 16/272 (5%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 ++ L I + + + ++ G Sbjct: 1 MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGA 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 + +I +S + + + Sbjct: 61 SVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGL 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 V + A + +Q +V + G Sbjct: 121 EAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGG 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 + +G + ++ A+ Sbjct: 181 KASDFVLAMGQGRMIPGFEDGIKGHK----AGEEFTID------------VTFPEEYHAE 224 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESII 272 + I++ R + + Sbjct: 225 NLKGKAAKFAINLKKVEERELPELTAEFIKRF 256 >BS_spoVB [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 192 bits (489), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 15/272 (5%), Positives = 41/272 (15%), Gaps = 16/272 (5%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 ++ L I + + + ++ G Sbjct: 1 MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGA 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 + +I +S + + + Sbjct: 61 SVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGL 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 V + A + +Q +V + G Sbjct: 121 EAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGG 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 + +G + + P K Sbjct: 181 KASDFVLAMGQGRMIPGFEDGIKGHKAG--EEFTIDVTFPEEYH-----AENLKG----- 228 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESII 272 I++ R + + Sbjct: 229 ----KAAKFAINLKKVEERELPELTAEFIKRF 256 >CAC3213 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 125 bits (315), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 13/273 (4%), Positives = 45/273 (16%), Gaps = 21/273 (7%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 ++ L I + + + ++ G Sbjct: 1 MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQ-RYG 59 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 + + + + + + + Sbjct: 60 ASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQG 119 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 P++ + +Q +E+ R+ S Sbjct: 120 LEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEG 179 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 A+ + I G + + K + + Sbjct: 180 GKASDFVLAMGQGRMIPGFEDGIKGH----KAGE----------------EFTIDVTFPE 219 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESIIT 273 + + + + V + Sbjct: 220 EYHAENLKGKAAKFAINLKKVEERELPELTAEF 252 >CAC1016 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 113 bits (283), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 13/257 (5%), Positives = 38/257 (14%), Gaps = 16/257 (6%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIG-AEGVGLYQLGYPFFIVLITIITG 59 ++ L I + + ++ G ++ Sbjct: 1 MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGA 60 Query: 60 GIPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSR 119 + + + + + V + Sbjct: 61 SVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYP-EVELQG 119 Query: 120 VYYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRG 179 + V + A + +Q +V + G Sbjct: 120 LEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEG 179 Query: 180 VAYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASA 239 + +G + E + Sbjct: 180 GKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTI----------DVTFPEEYHAENL--- 226 Query: 240 KSHSIFKRLIRISVPVT 256 K I + Sbjct: 227 -KGKAAKFAINLKKVEE 242 >BH3277 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 104 bits (260), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 8/221 (3%), Positives = 26/221 (11%), Gaps = 12/221 (5%) Query: 3 KQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGGIP 62 L I + + + ++ G Sbjct: 3 VSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASV 62 Query: 63 LAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVI------LT 116 + +I +S + Sbjct: 63 RQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEA 122 Query: 117 DSRVYYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLW--FSWL 174 + + + + ++ A V V Sbjct: 123 IEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKA 182 Query: 175 LLPRGVAYAAAG-AMLGVTVGEIVGMLALLWQYYYIVKRDK 214 + ++ Sbjct: 183 SDFVLAMGQGRMIPGFEDGIKGH-KAGEEFTIDVT--FPEE 220 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,322,070 Number of extensions: 710269 Number of successful extensions: 2447 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 2431 Number of HSP's successfully gapped: 23 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)