BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0019.p (300 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI0680_2 [U] COG0341 Preprotein translocase subunit SecF 177 1e-44 BMEI1076_2 [U] COG0341 Preprotein translocase subunit SecF 174 1e-43 SMc02265_2 [U] COG0341 Preprotein translocase subunit SecF 154 1e-37 SMc02057_2 [U] COG0341 Preprotein translocase subunit SecF 125 7e-29 STM0860 [GE] COG2610 H+/gluconate symporter and related permeases 55 2e-07 >BMEI0680_2 [U] COG0341 Preprotein translocase subunit SecF Length = 673 Score = 177 bits (450), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 11/294 (3%), Positives = 46/294 (15%), Gaps = 14/294 (4%) Query: 13 SRFFYI-FSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKPALKELGIS 71 +A +G + T + + + +A + + Sbjct: 12 EFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTL 71 Query: 72 HEPDITLGE-NRVNIRYD--EVLDSNQDEA---LKAAVTKLDDKASFE-INTVDTEMAKE 124 + + + + E + + Sbjct: 72 GRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVL 131 Query: 125 LARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDLTFIV 184 L + + + ++ + + Sbjct: 132 LQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPT 191 Query: 185 AVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFTVFIA 244 +L + + + LV V ++ I Sbjct: 192 LLLQANEEVRKRWQNKIRYLLVDEYQDTNTS---QYELVKLLVGSRARFTVVGDDDQSIY 248 Query: 245 AFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLK---KRQKQPVKS 295 ++ + + + + + Sbjct: 249 SWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFEKRL 302 >BMEI1076_2 [U] COG0341 Preprotein translocase subunit SecF Length = 673 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 22/291 (7%), Positives = 61/291 (20%), Gaps = 15/291 (5%) Query: 6 ELDFVHLSRFFYIFSIAITLAGLIFLATMGLNYSVDFKAGSNVDV-ALSKNITLEQLKPA 64 + S I +A L + A + + A + Q Sbjct: 19 LVLAGAGS--GKTRVITNKIA---HLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGR 73 Query: 65 LKELGISHEPDITLGENR---VNIRYDEVLDSNQDEALKAAVTKLDDKASFE-INTVDTE 120 + G+ TLG + + + + + + V + Sbjct: 74 KEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQ 133 Query: 121 MAKELARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDL 180 N + + R + A V+ + L L Sbjct: 134 QLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLL 193 Query: 181 TFIVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFT 240 + + +++ + N + L V Sbjct: 194 LQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVK---LLVGSRARFTVVGDDDQSIYSW 250 Query: 241 VFIAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLL--LKKRQ 289 L+++ + + + + + + +++ Sbjct: 251 RGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFEKR 301 >SMc02265_2 [U] COG0341 Preprotein translocase subunit SecF Length = 673 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 11/290 (3%), Positives = 47/290 (16%), Gaps = 17/290 (5%) Query: 13 SRFFYI-FSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKPALKELGIS 71 +A +G + T + + + +A + + Sbjct: 12 EFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTL 71 Query: 72 HEPDITL----GENRVNIRYDEVLDSNQDEAL-----KAAVTKLDDKASFE-INTVDTEM 121 + + +++ E + + Sbjct: 72 ---GRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDD 128 Query: 122 AKELARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDLT 181 L + + + ++ + + Sbjct: 129 KVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLIL 188 Query: 182 FIVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFTV 241 +L + + + + LV V ++ Sbjct: 189 LPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYE---LVKLLVGSRARFTVVGDDDQ 245 Query: 242 FIAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLKKRQKQ 291 I ++ + + + + Sbjct: 246 SIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNP 295 >SMc02057_2 [U] COG0341 Preprotein translocase subunit SecF Length = 673 Score = 125 bits (315), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 24/273 (8%), Positives = 61/273 (22%), Gaps = 12/273 (4%) Query: 35 GLNYSVDFKAGSNVDVALSKNIT-LEQLKPALKELGISHEPDITLGENR---VNIRYDEV 90 G + K + + + TLG + I Sbjct: 27 GKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHT 86 Query: 91 LDSNQDEALKAAVTKLDDKASFE-INTVDTEMAKELARNAIYSVLISCLGIIIYVSIRFE 149 L + + AA+ + + F+ + + VL+ L I Sbjct: 87 LGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDL 146 Query: 150 WRFALAAIVALVHDAFIVVAVFSIFRLEVDLTFIVAVLTIIGYSINDTIVIFDRIRENL- 208 + AA A+ I + ++ + ++ +I + + Sbjct: 147 KTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQANEEVRKRWQN 206 Query: 209 RFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFTVFIAAFFLLVMGGES-IKMFSLAMVIGL 267 + + + + +Q + L S +V+ Sbjct: 207 KIRYLL----VDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLS 262 Query: 268 LFGAYSSIFIASPLWLLLKKRQKQ-PVKSNPAK 299 + + + K + Sbjct: 263 QDFPALKVIKLEQNYRSSGRILKAANILIANNP 295 >STM0860 [GE] COG2610 H+/gluconate symporter and related permeases Length = 141 Score = 54.7 bits (130), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 0/198 (0%), Positives = 0/198 (0%), Gaps = 25/198 (12%) Query: 114 INTVDTEMAKELARNAIYSVL----ISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVA 169 Sbjct: 278 - 336 Query: 170 VFSIFRLEVDLTFIVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQ 229 Sbjct: 337 --------- 387 Query: 230 TLMRSLYTAF----TVFIAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIA---SPLW 282 Sbjct: 388 ---- 443 Query: 283 LLLKKRQKQPVKSNPAKA 300 Sbjct: 444 461 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.140 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,733,320 Number of extensions: 760441 Number of successful extensions: 2707 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 2714 Number of HSP's successfully gapped: 16 Length of query: 300 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 194 Effective length of database: 26,153,027 Effective search space: 5073687238 Effective search space used: 5073687238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)