BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0016.p (172 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1241 [F] COG0503 Adenine/guanine phosphoribosyltransferases an... 221 3e-58 BS_apt [F] COG0503 Adenine/guanine phosphoribosyltransferases an... 211 5e-55 lin1559 [F] COG0503 Adenine/guanine phosphoribosyltransferases a... 206 1e-53 CAC2275 [F] COG0503 Adenine/guanine phosphoribosyltransferases a... 201 4e-52 SA1461 [F] COG0503 Adenine/guanine phosphoribosyltransferases an... 197 5e-51 >BH1241 [F] COG0503 Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins Length = 171 Score = 221 bits (564), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 105/171 (61%), Positives = 132/171 (77%) Query: 1 MDFKDNIRVIPDFPQPGISFKDITTLLKDGEAYRRAIDELKALVAHLQIDVIAGPEARGF 60 MDFK +I ++ +FP+ GI FKDITTL+++GE Y+RAIDE+ ++D++ GPEARGF Sbjct: 1 MDFKQHITIVENFPKEGIRFKDITTLMQNGEVYKRAIDEMARFAKEKEVDLVVGPEARGF 60 Query: 61 VVGAPLAYALGVGFVPIRKSGKLPYDTIEVGYDLEYGKDTLAIHTDAVKPGQNVLIADDL 120 VVG P+AYA+G GFVP+RK+GKLP + I V Y LEYGKD L IH DA++ GQ VLIADDL Sbjct: 61 VVGCPIAYAIGKGFVPVRKAGKLPREVISVDYGLEYGKDRLTIHRDAIQKGQRVLIADDL 120 Query: 121 LATGGTIATSVSLVKQLGGNVVGAAFLIELTQLAGRNKLSGIEVHSLLTYE 171 LATGGTI +V +V+QLGG VVG AF+IEL L GR+KL +V SL TYE Sbjct: 121 LATGGTIEATVQMVEQLGGEVVGIAFMIELGYLNGRDKLVTYDVFSLTTYE 171 >BS_apt [F] COG0503 Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins Length = 170 Score = 211 bits (537), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 98/170 (57%), Positives = 126/170 (74%) Query: 1 MDFKDNIRVIPDFPQPGISFKDITTLLKDGEAYRRAIDELKALVAHLQIDVIAGPEARGF 60 MD K + ++PD+P+ G+ FKDITTL+ G+ YR A D++ QID++ GPEARGF Sbjct: 1 MDLKQYVTIVPDYPKEGVQFKDITTLMDKGDVYRYATDQIVEYAKEKQIDLVVGPEARGF 60 Query: 61 VVGAPLAYALGVGFVPIRKSGKLPYDTIEVGYDLEYGKDTLAIHTDAVKPGQNVLIADDL 120 ++G P+AYALGVGF P+RK GKLP + I+V Y LEYGKD L IH DA+KPGQ VLI DDL Sbjct: 61 IIGCPVAYALGVGFAPVRKEGKLPREVIKVDYGLEYGKDVLTIHKDAIKPGQRVLITDDL 120 Query: 121 LATGGTIATSVSLVKQLGGNVVGAAFLIELTQLAGRNKLSGIEVHSLLTY 170 LATGGTI ++ LV++LGG V G AFLIEL+ L GRNKL ++ +L+ Y Sbjct: 121 LATGGTIEATIKLVEELGGVVAGIAFLIELSYLDGRNKLEDYDILTLMKY 170 >lin1559 [F] COG0503 Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins Length = 301 Score = 206 bits (524), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 9/160 (5%), Positives = 22/160 (13%) Query: 2 DFKDNIRVIPDFPQPGISFKDITTLLKDGEAYRRAIDELKALVAHLQIDVIAGPEARGFV 61 I P G S L + R + + + + Sbjct: 5 KSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTP 64 Query: 62 VGAPLAYALGVGFVPIRKSGKLPYDTIEVGYDLEYGKDTLAIHTDAVKPGQNVLIADDLL 121 + S + + + + + Sbjct: 65 GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVN 124 Query: 122 ATGGTIATSVSLVKQLGGNVVGAAFLIELTQLAGRNKLSG 161 + L I + Sbjct: 125 KVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDT 164 >CAC2275 [F] COG0503 Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins Length = 172 Score = 201 bits (512), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 94/170 (55%), Positives = 126/170 (74%) Query: 1 MDFKDNIRVIPDFPQPGISFKDITTLLKDGEAYRRAIDELKALVAHLQIDVIAGPEARGF 60 MD KD+IRVI FP+ GISFKD+TTL++D EAY+ ++D++ + +D+I PEARGF Sbjct: 1 MDLKDSIRVIDGFPKEGISFKDVTTLIQDKEAYKYSVDKIGEYLKDKNVDLIVAPEARGF 60 Query: 61 VVGAPLAYALGVGFVPIRKSGKLPYDTIEVGYDLEYGKDTLAIHTDAVKPGQNVLIADDL 120 + GAP+AY +G GF+P+RK GKLP +T+EV Y+LEYG+D L +H DA+K GQ V I DDL Sbjct: 61 LFGAPVAYKIGAGFIPVRKKGKLPCETVEVTYELEYGEDILEMHKDAIKKGQRVAIVDDL 120 Query: 121 LATGGTIATSVSLVKQLGGNVVGAAFLIELTQLAGRNKLSGIEVHSLLTY 170 LATGGTI + LV+ LGG VV A F++ELT L G++KL + SL+ Y Sbjct: 121 LATGGTINSVAKLVEALGGEVVAACFVVELTDLNGKDKLGDYDTMSLVKY 170 >SA1461 [F] COG0503 Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins Length = 172 Score = 197 bits (502), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 90/172 (52%), Positives = 125/172 (72%) Query: 1 MDFKDNIRVIPDFPQPGISFKDITTLLKDGEAYRRAIDELKALVAHLQIDVIAGPEARGF 60 MD K + + D+P+PG+SFKDITT++ +GEAY A D++ +D++ GPEARGF Sbjct: 1 MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYAKDRDVDIVVGPEARGF 60 Query: 61 VVGAPLAYALGVGFVPIRKSGKLPYDTIEVGYDLEYGKDTLAIHTDAVKPGQNVLIADDL 120 ++G P+AY++G+GF P+RK GKLP + I YDLEYG + L +H DA+KPGQ VLI DDL Sbjct: 61 IIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLTMHKDAIKPGQRVLITDDL 120 Query: 121 LATGGTIATSVSLVKQLGGNVVGAAFLIELTQLAGRNKLSGIEVHSLLTYED 172 LATGGTI ++ LV++LGG VVG AF+IEL L G K+ +V SL++Y++ Sbjct: 121 LATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIKDYDVMSLISYDE 172 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.141 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,673,929 Number of extensions: 605952 Number of successful extensions: 1482 Number of sequences better than 1.0e-05: 64 Number of HSP's gapped: 1485 Number of HSP's successfully gapped: 64 Length of query: 172 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 73 Effective length of database: 26,960,365 Effective search space: 1968106645 Effective search space used: 1968106645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 112 (47.8 bits)