BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0015.p (434 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM2497 [F] COG2233 Xanthine/uracil permeases 497 e-140 ZuraA [F] COG2233 Xanthine/uracil permeases 497 e-140 uraA [F] COG2233 Xanthine/uracil permeases 497 e-140 ZuraA [F] COG2233 Xanthine/uracil permeases 497 e-140 ECs3359 [F] COG2233 Xanthine/uracil permeases 497 e-140 >STM2497 [F] COG2233 Xanthine/uracil permeases Length = 211 Score = 497 bits (1280), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/436 (0%), Positives = 0/436 (0%), Gaps = 13/436 (2%) Query: 2 QREIQVNEKLPWGPGFLLSLQHLFAMFGSTVLVPNLFGVDPGMILLMNGIGTLLYILICR 61 + + + + F+ ++ L + + Sbjct: 3 KAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPA 62 Query: 62 GKIPAYLGSSFAFISPVLGVLEKHQGDHQHGYSLALGAFIVTGVIFILVALIVRYAGTGW 121 + + +I + K ++ I+ V Sbjct: 63 AMLELGVEGVKTYIKTIGLYNSK-------AENIIKTCRILLEQHNGEVPEDRAALEALP 115 Query: 122 IDVVFPPAVMGAIVATIGLELVPVAARMSGLIAPEGATDWTPDGKAITLSMVTLGVTVIG 181 V+ V + + + ++ + Sbjct: 116 GVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPG---KNVEQVEEKLLKVVPAEFK 172 Query: 182 AVLFRGFPKIIHILIGIVTGYVLAYIMQVVNTDAISSAHFFSHPTIITPSFDWQVIFTII 241 Sbjct: 173 232 Query: 242 PVSLVVIVEHIGHLLVTSNIVGKDLTKEPGLDRSLMGNGISTILSGFFGSTPNTTYGENI 301 Sbjct: 233 292 Query: 302 GVMALTKVYSVYVIGGAAVIAILLSFSGTFSSIIANIPQPVMGGVSLLLFGVIAASGLRI 361 Sbjct: 293 352 Query: 362 FVEQKVDFSKATNMILATLVLVVGISGISLTIYGVQLKGMALATIVGMVLSLLFKLIEVL 421 Sbjct: 353 412 Query: 422 GLSN---EQDSEKSAH 434 Sbjct: 413 428 >ZuraA [F] COG2233 Xanthine/uracil permeases Length = 211 Score = 497 bits (1279), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/420 (0%), Positives = 0/420 (0%), Gaps = 10/420 (2%) Query: 2 QREIQVNEKLPWGPGFLLSLQHLFAMFGSTVLVPNLFGVDPGMILLMNGIGTLLYILICR 61 + + + + F+ ++ L + + Sbjct: 3 KAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPA 62 Query: 62 GKIPAYLGSSFAFISPVLGVLEKHQGDHQHGYSLALGAFIVTGVIFILVALIVRYAGTGW 121 + + +I + K ++ I+ V Sbjct: 63 AMLELGVEGVKTYIKTIGLYNSK-------AENIIKTCRILLEQHNGEVPEDRAALEALP 115 Query: 122 IDVVFPPAVMGAIVATIGLELVPVAARMSGLIAPEGATDWTPDGKAITLSMVTLGVTVIG 181 V+ V + + + ++ + Sbjct: 116 GVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPG---KNVEQVEEKLLKVVPAEFK 172 Query: 182 AVLFRGFPKIIHILIGIVTGYVLAYIMQVVNTDAISSAHFFSHPTIITPSFDWQVIFTII 241 Sbjct: 173 232 Query: 242 PVSLVVIVEHIGHLLVTSNIVGKDLTKEPGLDRSLMGNGISTILSGFFGSTPNTTYGENI 301 Sbjct: 233 292 Query: 302 GVMALTKVYSVYVIGGAAVIAILLSFSGTFSSIIANIPQPVMGGVSLLLFGVIAASGLRI 361 Sbjct: 293 352 Query: 362 FVEQKVDFSKATNMILATLVLVVGISGISLTIYGVQLKGMALATIVGMVLSLLFKLIEVL 421 Sbjct: 353 412 >uraA [F] COG2233 Xanthine/uracil permeases Length = 429 Score = 497 bits (1279), Expect = e-140, Method: Compositional matrix adjust. Identities = 259/420 (61%), Positives = 327/420 (77%), Gaps = 10/420 (2%) Query: 2 QREIQVNEKLPWGPGFLLSLQHLFAMFGSTVLVPNLFGVDPGMILLMNGIGTLLYILICR 61 +R I V+E+ P LSLQHLFAMFG+TVLVP LF ++P +LL NGIGTLLY+ IC+ Sbjct: 3 RRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICK 62 Query: 62 GKIPAYLGSSFAFISPVLGVLEKHQGDHQHGYSLALGAFIVTGVIFILVALIVRYAGTGW 121 GKIPAYLGSSFAFISPVL +L GY +ALG FI+ GV+F LV+ IV+ AGTGW Sbjct: 63 GKIPAYLGSSFAFISPVLLLLPL-------GYEVALGGFIMCGVLFCLVSFIVKKAGTGW 115 Query: 122 IDVVFPPAVMGAIVATIGLELVPVAARMSGLIAPEGATDWTPDGKAITLSMVTLGVTVIG 181 +DV+FPPA MGAIVA IGLEL VAA M+GL+ EG T PD K I +S+ TL VTV+G Sbjct: 116 LDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQT---PDSKTIIISITTLAVTVLG 172 Query: 182 AVLFRGFPKIIHILIGIVTGYVLAYIMQVVNTDAISSAHFFSHPTIITPSFDWQVIFTII 241 +VLFRGF II ILIG++ GY L++ M +V+T I +AH+F+ PT+ TP F+W I TI+ Sbjct: 173 SVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTIL 232 Query: 242 PVSLVVIVEHIGHLLVTSNIVGKDLTKEPGLDRSLMGNGISTILSGFFGSTPNTTYGENI 301 P +LVVI EH+GHL+VT+NIV KDL ++PGL RS+ NG+ST++SGFFGSTPNTTYGENI Sbjct: 233 PAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENI 292 Query: 302 GVMALTKVYSVYVIGGAAVIAILLSFSGTFSSIIANIPQPVMGGVSLLLFGVIAASGLRI 361 GVMA+T+VYS +VIGGAA+ AILLS G ++ I IP PVMGGVSLLL+GVI ASG+R+ Sbjct: 293 GVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRV 352 Query: 362 FVEQKVDFSKATNMILATLVLVVGISGISLTIYGVQLKGMALATIVGMVLSLLFKLIEVL 421 +E KVD++KA N+IL +++L++G+SG + I +LKGMALATIVG+ LSL+FKLI VL Sbjct: 353 LIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGIGLSLIFKLISVL 412 Score = 497 bits (1279), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/420 (0%), Positives = 0/420 (0%), Gaps = 10/420 (2%) Query: 2 QREIQVNEKLPWGPGFLLSLQHLFAMFGSTVLVPNLFGVDPGMILLMNGIGTLLYILICR 61 + + + + F+ ++ L + + Sbjct: 3 KAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPA 62 Query: 62 GKIPAYLGSSFAFISPVLGVLEKHQGDHQHGYSLALGAFIVTGVIFILVALIVRYAGTGW 121 + + +I + K ++ I+ V Sbjct: 63 AMLELGVEGVKTYIKTIGLYNSK-------AENIIKTCRILLEQHNGEVPEDRAALEALP 115 Query: 122 IDVVFPPAVMGAIVATIGLELVPVAARMSGLIAPEGATDWTPDGKAITLSMVTLGVTVIG 181 V+ V + + + ++ + Sbjct: 116 GVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPG---KNVEQVEEKLLKVVPAEFK 172 Query: 182 AVLFRGFPKIIHILIGIVTGYVLAYIMQVVNTDAISSAHFFSHPTIITPSFDWQVIFTII 241 Sbjct: 173 232 Query: 242 PVSLVVIVEHIGHLLVTSNIVGKDLTKEPGLDRSLMGNGISTILSGFFGSTPNTTYGENI 301 Sbjct: 233 292 Query: 302 GVMALTKVYSVYVIGGAAVIAILLSFSGTFSSIIANIPQPVMGGVSLLLFGVIAASGLRI 361 Sbjct: 293 352 Query: 362 FVEQKVDFSKATNMILATLVLVVGISGISLTIYGVQLKGMALATIVGMVLSLLFKLIEVL 421 Sbjct: 353 412 >ECs3359 [F] COG2233 Xanthine/uracil permeases Length = 211 Score = 497 bits (1279), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/420 (0%), Positives = 0/420 (0%), Gaps = 10/420 (2%) Query: 2 QREIQVNEKLPWGPGFLLSLQHLFAMFGSTVLVPNLFGVDPGMILLMNGIGTLLYILICR 61 + + + + F+ ++ L + + Sbjct: 3 KAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPA 62 Query: 62 GKIPAYLGSSFAFISPVLGVLEKHQGDHQHGYSLALGAFIVTGVIFILVALIVRYAGTGW 121 + + +I + K ++ I+ V Sbjct: 63 AMLELGVEGVKTYIKTIGLYNSK-------AENIIKTCRILLEQHNGEVPEDRAALEALP 115 Query: 122 IDVVFPPAVMGAIVATIGLELVPVAARMSGLIAPEGATDWTPDGKAITLSMVTLGVTVIG 181 V+ V + + + ++ + Sbjct: 116 GVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPG---KNVEQVEEKLLKVVPAEFK 172 Query: 182 AVLFRGFPKIIHILIGIVTGYVLAYIMQVVNTDAISSAHFFSHPTIITPSFDWQVIFTII 241 Sbjct: 173 232 Query: 242 PVSLVVIVEHIGHLLVTSNIVGKDLTKEPGLDRSLMGNGISTILSGFFGSTPNTTYGENI 301 Sbjct: 233 292 Query: 302 GVMALTKVYSVYVIGGAAVIAILLSFSGTFSSIIANIPQPVMGGVSLLLFGVIAASGLRI 361 Sbjct: 293 352 Query: 362 FVEQKVDFSKATNMILATLVLVVGISGISLTIYGVQLKGMALATIVGMVLSLLFKLIEVL 421 Sbjct: 353 412 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.143 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,448,134 Number of extensions: 1494038 Number of successful extensions: 5229 Number of sequences better than 1.0e-05: 71 Number of HSP's gapped: 5033 Number of HSP's successfully gapped: 71 Length of query: 434 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 325 Effective length of database: 25,807,025 Effective search space: 8387283125 Effective search space used: 8387283125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)