BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0009.p (417 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_1229 [GEPR] COG0477 Permeases of the major facilitator superf... 326 4e-89 aq_1220 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and ... 326 4e-89 aq_1224 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 326 4e-89 TM1090 [J] COG0124 Histidyl-tRNA synthetase 316 4e-86 YPR033c [J] COG0124 Histidyl-tRNA synthetase 53 1e-06 >aq_1229 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 403 Score = 326 bits (835), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 176/404 (43%), Positives = 239/404 (59%), Gaps = 10/404 (2%) Query: 11 GTQDVLPGAVEKWLVVESKARDLCRRFNYREIRTPMFEHTGLFERGVGETTDIVEGEMYT 70 G D+L +K+ + AR + + +N+ EI P+ E+ LF+R VGETTDIV+ EM+ Sbjct: 8 GFHDILGKDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFV 67 Query: 71 FKDKGDRDLALRPEGTAGVVRAYVQNKLYGEPDVSKLYYIGPMFRYERPQAGRYRQFHQF 130 F+D+ R LALRPEGTAG VRA++Q+KLY KL+Y GPMFRYERPQAGRYRQFHQ Sbjct: 68 FEDRKGRKLALRPEGTAGTVRAFIQHKLYALRPYVKLFYEGPMFRYERPQAGRYRQFHQI 127 Query: 131 GIEAFGAVDPAIDAEVVSMGYQFYKDLGLKDVKVELNSVGNAPSRAAYREKLLNFLRPMR 190 G E FG +P DAE++ + Y + LG+K V VE+NS+G R AYRE LLN+L ++ Sbjct: 128 GAEVFGVAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGVK 187 Query: 191 DTLCSDCQRRMERNPLRVLDCKVDQDKFG--GAPSILDSLDEECTTHFEQVKAHLDVMGV 248 + LCSDC RM+RNPLRVLDCKV+ K AP ++D L +EC H+E++K +L + + Sbjct: 188 EELCSDCISRMDRNPLRVLDCKVETCKVAVREAPKMIDFLCDECREHYEKLKNYLKALDI 247 Query: 249 EYSINPRLVRGLDYYTHTAFEYKAAXXXXXXXXXXXXRYNGLVEEIGGPDQPXXXXXXXX 308 + N LVRGLDYYT T FE A RY+ LVEE+GGP P Sbjct: 248 PFRENYNLVRGLDYYTRTVFE--AVSDELGLTLIAGGRYDYLVEELGGPPTPALGFALGV 305 Query: 309 XXXXXXXENQGVELEAAKPLDVYFVALGEAADKEITKQLFTLRSLGYAAERDYLGRKMKA 368 ++ + +VYFV + + LR G E Y +K Sbjct: 306 ERLMLLLPDE------EEKEEVYFVIPFGDVHEYALRVADILRKKGKVVEYSYRKGGLKK 359 Query: 369 QMKSADRMNARYTAILGEDELNSGVIALKSMATGEQRTVKLEDL 412 Q++ AD++ +Y I+GEDE+ + + +K M TGEQR VKL +L Sbjct: 360 QLEFADKLGVKYAVIIGEDEVKNQEVTIKDMETGEQRRVKLSEL 403 >aq_1220 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family Length = 403 Score = 326 bits (835), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 176/404 (43%), Positives = 239/404 (59%), Gaps = 10/404 (2%) Query: 11 GTQDVLPGAVEKWLVVESKARDLCRRFNYREIRTPMFEHTGLFERGVGETTDIVEGEMYT 70 G D+L +K+ + AR + + +N+ EI P+ E+ LF+R VGETTDIV+ EM+ Sbjct: 8 GFHDILGKDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFV 67 Query: 71 FKDKGDRDLALRPEGTAGVVRAYVQNKLYGEPDVSKLYYIGPMFRYERPQAGRYRQFHQF 130 F+D+ R LALRPEGTAG VRA++Q+KLY KL+Y GPMFRYERPQAGRYRQFHQ Sbjct: 68 FEDRKGRKLALRPEGTAGTVRAFIQHKLYALRPYVKLFYEGPMFRYERPQAGRYRQFHQI 127 Query: 131 GIEAFGAVDPAIDAEVVSMGYQFYKDLGLKDVKVELNSVGNAPSRAAYREKLLNFLRPMR 190 G E FG +P DAE++ + Y + LG+K V VE+NS+G R AYRE LLN+L ++ Sbjct: 128 GAEVFGVAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGVK 187 Query: 191 DTLCSDCQRRMERNPLRVLDCKVDQDKFG--GAPSILDSLDEECTTHFEQVKAHLDVMGV 248 + LCSDC RM+RNPLRVLDCKV+ K AP ++D L +EC H+E++K +L + + Sbjct: 188 EELCSDCISRMDRNPLRVLDCKVETCKVAVREAPKMIDFLCDECREHYEKLKNYLKALDI 247 Query: 249 EYSINPRLVRGLDYYTHTAFEYKAAXXXXXXXXXXXXRYNGLVEEIGGPDQPXXXXXXXX 308 + N LVRGLDYYT T FE A RY+ LVEE+GGP P Sbjct: 248 PFRENYNLVRGLDYYTRTVFE--AVSDELGLTLIAGGRYDYLVEELGGPPTPALGFALGV 305 Query: 309 XXXXXXXENQGVELEAAKPLDVYFVALGEAADKEITKQLFTLRSLGYAAERDYLGRKMKA 368 ++ + +VYFV + + LR G E Y +K Sbjct: 306 ERLMLLLPDE------EEKEEVYFVIPFGDVHEYALRVADILRKKGKVVEYSYRKGGLKK 359 Query: 369 QMKSADRMNARYTAILGEDELNSGVIALKSMATGEQRTVKLEDL 412 Q++ AD++ +Y I+GEDE+ + + +K M TGEQR VKL +L Sbjct: 360 QLEFADKLGVKYAVIIGEDEVKNQEVTIKDMETGEQRRVKLSEL 403 >aq_1224 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Length = 403 Score = 326 bits (835), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 176/404 (43%), Positives = 239/404 (59%), Gaps = 10/404 (2%) Query: 11 GTQDVLPGAVEKWLVVESKARDLCRRFNYREIRTPMFEHTGLFERGVGETTDIVEGEMYT 70 G D+L +K+ + AR + + +N+ EI P+ E+ LF+R VGETTDIV+ EM+ Sbjct: 8 GFHDILGKDAKKFRKISDTARKILKLYNFEEIILPVVEYAELFQRSVGETTDIVQKEMFV 67 Query: 71 FKDKGDRDLALRPEGTAGVVRAYVQNKLYGEPDVSKLYYIGPMFRYERPQAGRYRQFHQF 130 F+D+ R LALRPEGTAG VRA++Q+KLY KL+Y GPMFRYERPQAGRYRQFHQ Sbjct: 68 FEDRKGRKLALRPEGTAGTVRAFIQHKLYALRPYVKLFYEGPMFRYERPQAGRYRQFHQI 127 Query: 131 GIEAFGAVDPAIDAEVVSMGYQFYKDLGLKDVKVELNSVGNAPSRAAYREKLLNFLRPMR 190 G E FG +P DAE++ + Y + LG+K V VE+NS+G R AYRE LLN+L ++ Sbjct: 128 GAEVFGVAEPHADAEIIKIVYDILQALGIKGVVVEINSLGCKKDREAYREALLNYLTGVK 187 Query: 191 DTLCSDCQRRMERNPLRVLDCKVDQDKFG--GAPSILDSLDEECTTHFEQVKAHLDVMGV 248 + LCSDC RM+RNPLRVLDCKV+ K AP ++D L +EC H+E++K +L + + Sbjct: 188 EELCSDCISRMDRNPLRVLDCKVETCKVAVREAPKMIDFLCDECREHYEKLKNYLKALDI 247 Query: 249 EYSINPRLVRGLDYYTHTAFEYKAAXXXXXXXXXXXXRYNGLVEEIGGPDQPXXXXXXXX 308 + N LVRGLDYYT T FE A RY+ LVEE+GGP P Sbjct: 248 PFRENYNLVRGLDYYTRTVFE--AVSDELGLTLIAGGRYDYLVEELGGPPTPALGFALGV 305 Query: 309 XXXXXXXENQGVELEAAKPLDVYFVALGEAADKEITKQLFTLRSLGYAAERDYLGRKMKA 368 ++ + +VYFV + + LR G E Y +K Sbjct: 306 ERLMLLLPDE------EEKEEVYFVIPFGDVHEYALRVADILRKKGKVVEYSYRKGGLKK 359 Query: 369 QMKSADRMNARYTAILGEDELNSGVIALKSMATGEQRTVKLEDL 412 Q++ AD++ +Y I+GEDE+ + + +K M TGEQR VKL +L Sbjct: 360 QLEFADKLGVKYAVIIGEDEVKNQEVTIKDMETGEQRRVKLSEL 403 >TM1090 [J] COG0124 Histidyl-tRNA synthetase Length = 346 Score = 316 bits (810), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 0/410 (0%), Positives = 0/410 (0%), Gaps = 1/410 (0%) Query: 5 RFEKPTGTQDVLPGAVEKWLVVESKARDLCRRFNYREIRTPMFEHTGLFERGVGETTDIV 64 ++ + + + + + + + E Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 65 EGEMYTFKDKGDRDLALRPEGTAGVVRAYVQNKLYGEPDVSKLYYIGPMFRYERPQAGRY 124 +G T+ R + + + + R Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 125 RQFHQFGIEAFGAVDPAIDAEVVSMGYQFYKDLGLKDVKVELNSVGNAPSRAAYREKLLN 184 + + + P + + ++ + + Y Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 185 FLRPMRDTLCSDCQRRMERNPLRVLDCKVDQDKFGGAPSILDSLDEECTTHFEQVKAHLD 244 + L + + R + F + + + + + Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 245 VMGVEYSINPRLVRGLDYYTHTAFEYKAAXXXXXXXXXXXXRYNGLVEEIGGPDQPXXXX 304 P R + T + + R+ ++ Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Query: 305 XXXXXXXXXXXENQGVELEAAKPLDVYFVALGEAADKEITKQLFTLRSLGYAAERDYLGR 364 Sbjct: 302 361 Query: 365 KMKAQMKSADRMNARYTAILGEDELNSGVIALKSMATGEQRTVKLEDLAA 414 Sbjct: 362 - 410 >YPR033c [J] COG0124 Histidyl-tRNA synthetase Length = 346 Score = 52.8 bits (125), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 17/302 (5%), Positives = 34/302 (11%), Gaps = 57/302 (18%) Query: 6 FEKPTGTQDVLPGAVEKWLVVESKARDLCRRFNYREIRTPMFEHTGLFERGVGETTDIVE 65 TQ + V WL L + + + Sbjct: 55 VPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREA- 113 Query: 66 GEMYTFKDKGDRDLALRPEGTAGVVRAYVQNKLYGEPDVSKLYYIGPMFRYERP--QAGR 123 K + L R G P Sbjct: 114 ---LHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLG----YSWTDSAPAVSLLDTLDALAE 166 Query: 124 YRQFHQFGIEAFGAVDPAI-DAEVVSMGYQFYKDLGLKDVKVELNSVGNAPSRAAYREKL 182 Y++ + + + L Sbjct: 167 YQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLL-----------NRSF 215 Query: 183 LNFLRPMRDTLCSDCQR------RMERNPLRVLDCKVDQ---------DKFGGAPSILDS 227 L + + G + Sbjct: 216 EVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVA 275 Query: 228 LDE--------ECTTHFEQVKAHLDVMG--VEY----------SINPRLVRGLDYYTHTA 267 + + + + L D Sbjct: 276 AYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIE 335 Query: 268 FE 269 Sbjct: 336 AV 337 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,048,242 Number of extensions: 1177678 Number of successful extensions: 1600 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1591 Number of HSP's successfully gapped: 5 Length of query: 417 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 308 Effective length of database: 25,807,025 Effective search space: 7948563700 Effective search space used: 7948563700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)