BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0007.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YHR003c [H] COG1179 Dinucleotide-utilizing enzymes involved in m... 123 3e-28 YKL027w [H] COG1179 Dinucleotide-utilizing enzymes involved in m... 115 7e-26 VCA0618 [H] COG0476 Dinucleotide-utilizing enzymes involved in m... 83 5e-16 Cgl1994_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in... 80 2e-15 ZmoeB [H] COG0476 Dinucleotide-utilizing enzymes involved in mol... 75 7e-14 >YHR003c [H] COG1179 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1 Length = 429 Score = 123 bits (309), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 70/201 (34%), Positives = 110/201 (54%), Gaps = 4/201 (1%) Query: 4 QFSRTELAIGPEGLEIMKNSTVAVLGIGGVGAMAVEALARTGIGRIILIDKDSVDITNIN 63 Q +R +G EG+ +K + ++G G VG+ L R+G +I++ID +++ I ++N Sbjct: 56 QLARNYAFLGEEGMRKIKEQYIVIVGAGEVGSWVCTMLIRSGCQKIMIIDPENISIDSLN 115 Query: 64 RQIHALTTTIGQNKTDLMVERIKLINPECEAIALNMFYTEETYEELFKYKLD---YVIDA 120 A+ + IG+ K + E + I P E A +T+E +L + +++D Sbjct: 116 THCCAVLSDIGKPKVQCLKEHLSKIAPWSEIKARAKAWTKENSHDLIFADGESPTFIVDC 175 Query: 121 SDTIIYKVHIIKECLARGIPMISSMGAANKMDPTRFQVADISKTSMDPIARVIRQKLRKE 180 D + KV +++ I +ISSMG A K DPTR + DIS T DPI+R +R+KLRK Sbjct: 176 LDNLESKVDLLEYAHHNKIDVISSMGVATKSDPTRVSINDISMTEFDPISRCVRRKLRKR 235 Query: 181 GIKKGVKVVFSTEEPVKPRED 201 GI G+ VVFS E + PR D Sbjct: 236 GIATGISVVFSN-EMLDPRRD 255 >YKL027w [H] COG1179 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1 Length = 447 Score = 115 bits (287), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 74/200 (37%), Positives = 115/200 (57%), Gaps = 7/200 (3%) Query: 3 HQFSRTELA-----IGPEGLEIMKNSTVAVLGIGGVGAMAVEALARTGIGRIILIDKDSV 57 QF R L +G + +E + N V V+G GGVG+ V +L R+G +I ++D D V Sbjct: 62 EQFIRQSLKNNVEFLGEDTIEKLSNQYVVVVGAGGVGSWVVNSLVRSGCRKIRVVDFDQV 121 Query: 58 DITNINRQIHALTTTIGQNKTDLMVERIKLINPECEAIALNMFYTEETYEELF--KYKLD 115 ++++NR A+ +G K + + ++ I P CE +N +T + E L D Sbjct: 122 SLSSLNRHSCAILNDVGTPKVECLRRHMREIAPWCEIDPINELWTLQNGERLTLGNGTPD 181 Query: 116 YVIDASDTIIYKVHIIKECLARGIPMISSMGAANKMDPTRFQVADISKTSMDPIARVIRQ 175 +++D D I KV +++ GI +ISSMGA+ K DPT+ V D++ T DP+ARV+R+ Sbjct: 182 FIVDCIDNIDTKVDLLEFAYNHGIKVISSMGASAKSDPTKLNVGDLATTEEDPLARVVRR 241 Query: 176 KLRKEGIKKGVKVVFSTEEP 195 KL+K GI G+ VVFS E+P Sbjct: 242 KLKKRGILSGIPVVFSAEKP 261 >VCA0618 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 254 Score = 82.8 bits (203), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 45/138 (32%), Positives = 77/138 (55%), Gaps = 3/138 (2%) Query: 15 EGLEIMKNSTVAVLGIGGVGAMAVEALARTGIGRIILIDKDSVDITNINRQIHALTTTIG 74 EG E +K STV +LG GG+G + + LA GIG I L+D D V+++N+ RQ+ IG Sbjct: 26 EGQEKLKQSTVLILGAGGLGCASGQYLATAGIGHITLVDDDVVELSNLQRQVLHHDADIG 85 Query: 75 QNKTDLMVERIKLINPECEAIALNMFYTEETYEELFKYKLDYVIDASDTIIYKVHIIKEC 134 + K D + ++L+NP + + +++ + L + D V+DA D + + + + C Sbjct: 86 RAKVDSAADSLRLLNPHLQVETIQARLSDDELDALIA-RHDLVLDACDNVGTRNQLNRLC 144 Query: 135 LARGIPMISSMGAANKMD 152 P++S GAA +M+ Sbjct: 145 FKHKTPLVS--GAAIRME 160 >Cgl1994_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 277 Score = 80.5 bits (197), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 52/144 (36%), Positives = 82/144 (56%), Gaps = 5/144 (3%) Query: 2 LHQFSRTELAI---GPEGLEIMKNSTVAVLGIGGVGAMAVEALARTGIGRIILIDKDSVD 58 LH+ +R +LA+ G E E + N+ V V+G GG+G +++LA G+G I +ID D+VD Sbjct: 9 LHRTAR-QLALPGYGIEQQERLFNAHVLVIGAGGLGCPVMQSLASAGVGTITVIDDDTVD 67 Query: 59 ITNINRQIHALTTTIGQNKTDLMVERIKLINPECEAIALNMFYTEETYEELFKYKLDYVI 118 I+NI+RQI + +G+ K ++ ER+K + P+ AL+ T E EL +D V+ Sbjct: 68 ISNIHRQILFGASDVGRPKVEVAAERLKELQPDITVNALHERITPENACELLN-SVDLVL 126 Query: 119 DASDTIIYKVHIIKECLARGIPMI 142 D SD+ K + G P+I Sbjct: 127 DGSDSFSTKYLVSDAAEITGTPLI 150 >ZmoeB [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 211 Score = 75.5 bits (184), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 9/159 (5%), Positives = 27/159 (16%), Gaps = 6/159 (3%) Query: 15 EGLEIMKNSTVAVLGIGGVGAMAVEALARTGIGRIILIDKDSVDITNINRQIHALTTTIG 74 +AVL ++V + Sbjct: 24 LNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYN 83 Query: 75 QNKTDLMVERIKLINPECEAIALNMFYTEETYEELFKYKLDYVIDASDTIIYKVHIIKEC 134 +++ L+ + + E K + V++ + Sbjct: 84 SKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK-TANVVLNTAFGWPTIAVDTHIF 142 Query: 135 LARGIPMISSMGAANKMDPTRFQVADISKTSMDPIARVI 173 + N + + + Sbjct: 143 RVCNRTQFA--PGKNVEQV---EEKLLKVVPAEFKVDCH 176 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,536,155 Number of extensions: 645114 Number of successful extensions: 3185 Number of sequences better than 1.0e-05: 48 Number of HSP's gapped: 3172 Number of HSP's successfully gapped: 49 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)