BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0062.p (323 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2579 [H] COG1893 Ketopantoate reductase 94 3e-19 BS_ylbQ [H] COG1893 Ketopantoate reductase 93 5e-19 STM0434 [H] COG1893 Ketopantoate reductase 82 1e-15 PA4397 [H] COG1893 Ketopantoate reductase 75 1e-13 SA2232 [H] COG1893 Ketopantoate reductase 69 7e-12 >BH2579 [H] COG1893 Ketopantoate reductase Length = 346 Score = 93.6 bits (231), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 15/296 (5%), Positives = 44/296 (14%), Gaps = 35/296 (11%) Query: 34 EQAEELSRQGITISYEDGS-LPLHLTGSSFRAGNIDKFAETVIRVPGDWTAIMLKQNAFH 92 + A I ++ G + + A Sbjct: 37 DYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWL---------AKHGSREKLIIASK 87 Query: 93 DTLPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVTTEAAKRKSLTEIIHAG 152 + P + E L + + + Sbjct: 88 VSGPSRNNDKGIRPDQ---ALDRKNIREALHDS-LKRLQTDYLDLYQVHWPQRPTNCFGK 143 Query: 153 TGETFIGK----RRSINPAKGGLEEETAAISLTKALVAAGFRALMSKEVDTMIYRKLLFN 208 G ++ A + + + + + Sbjct: 144 LGYSWTDSAPAVSLLDTL-------------DALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 209 AVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAYDADAWEGILE-VCRA 267 + + + + R + + + G L Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGV---ELLAYSCLGFGTLTGKYLN 247 Query: 268 TSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAAPLHRWICRLVEGMNVKER 323 + + ++ A D A + Sbjct: 248 GAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 >BS_ylbQ [H] COG1893 Ketopantoate reductase Length = 346 Score = 93.2 bits (230), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 14/282 (4%), Positives = 47/282 (16%), Gaps = 29/282 (10%) Query: 25 EVRLWCRSDEQAEELSRQGITISYEDGSLPLHLTGSSFRAGNIDKFAETVIRVPGDWTAI 84 + + + + + + + Sbjct: 24 GEQNSEADAHAQLDYAVAQGI---------NLIDVAEMYP---VPPRPETQGLTETYVGN 71 Query: 85 MLKQNAFHDTLPKILAPL-RDARLYAVCFQNGNGHLELLQELLPQASVWVAVTTEAAKRK 143 L ++ + L + + + L ++ + +A + Sbjct: 72 WLAKH----GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 144 SLTEIIHAGTGETFIGKRRSINPAKGGLEEETAAISLTKALVAAGFRALMSKEVDTMIYR 203 + +A +++ I Sbjct: 128 LYQVHWPQRPTNC---FGKLGYSW------TDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 204 KLLFNAVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAYDADAWEGILE 263 + + + + + + E Y+ Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLA---EVSQYEGVELLAYSC 235 Query: 264 VCRATSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAA 305 + T A +R T +A A Sbjct: 236 LGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAY 277 >STM0434 [H] COG1893 Ketopantoate reductase Length = 346 Score = 82.0 bits (201), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 14/212 (6%), Positives = 32/212 (15%), Gaps = 19/212 (8%) Query: 112 FQNGNGHLELLQELLPQASVWVAVTTEAAKRKSLTEIIHAGTGETFIGKRRSINPAKGGL 171 + L ++ + Sbjct: 96 DKGIRPDQALDRKN--IREALHDSLKRLQTDYL-DLYQVHWPQRPTNCFGK--------- 143 Query: 172 EEETAAISLTKALVAAGFRALMSKEVDTMIYRKLLFNAVINPLTAIWRIQNGELTASGER 231 + S + AL + I + N + + + Sbjct: 144 LGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVT 203 Query: 232 IQLMKELYREAASVYDACGIAYDADAWEGILE-VCRATSGNTSSMLADVLASRATEIRWI 290 IQ ++ +L C T L + A + Sbjct: 204 IQNPYS---LLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFS 260 Query: 291 NGSIVAMADRAGIAAPLHRWICRLVEGMNVKE 322 + + A Sbjct: 261 RFTRYSGEQTQKAVAAYV---DIARRHGLDPA 289 >PA4397 [H] COG1893 Ketopantoate reductase Length = 346 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 8/200 (4%), Positives = 29/200 (14%), Gaps = 19/200 (9%) Query: 110 VCFQNGNGHLELLQELLPQASVWVAVTTEAAKRKSLTEIIHAGTGETFIGKRRSINPAKG 169 + + + L + + + + + Sbjct: 99 IRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPA---- 154 Query: 170 GLEEETAAISLTKALVAAGFRALMSKEVDTMIYRKLLFNAVINPLTAIWRIQNGELTASG 229 ++ T A ++ + L + + Sbjct: 155 ------VSLLDTL-DALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNP 207 Query: 230 ERIQLMKELYREAASVYDACGIAYDADA---WEGILEVCRATSGNTSSMLADVLASRATE 286 + E + + N + + Sbjct: 208 YSLLNRSF---EVGLAEVSQY--EGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRF 262 Query: 287 IRWINGSIVAMADRAGIAAP 306 R+ A Sbjct: 263 TRYSGEQTQKAVAAYVDIAR 282 >SA2232 [H] COG1893 Ketopantoate reductase Length = 346 Score = 69.3 bits (168), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 15/284 (5%), Positives = 47/284 (16%), Gaps = 19/284 (6%) Query: 35 QAEELSRQGITISYEDGSLPLHLTGSSFRAGNIDKFAETVIRVPGDWTAIMLKQNAFHDT 94 Q + QGI + P+ + ++ + Sbjct: 35 QLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGS---REKLIIA-SKVSG 90 Query: 95 LPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVTTEAAKRKSLTEIIHAGTG 154 + + + ++ + + G Sbjct: 91 PSRN-----NDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLG 145 Query: 155 ETFIGKRRSINPAKGGLEEETAAISLTKALVAAGFRALMSKEVDTMIYRKLLFNAVINPL 214 ++ + + AG + +T + Sbjct: 146 YSWTDSAPA--------VSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHD 197 Query: 215 TAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAYDAD-AWEGILEVCRATSGNTS 273 + ++ + V L + + Sbjct: 198 LPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNT 257 Query: 274 SMLAD-VLASRATEIRWINGSIVAMADRAGIAAPLHRWICRLVE 316 + T+ +A A ++ R Sbjct: 258 LFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,072,542 Number of extensions: 847440 Number of successful extensions: 1768 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 1759 Number of HSP's successfully gapped: 9 Length of query: 323 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 216 Effective length of database: 26,037,693 Effective search space: 5624141688 Effective search space used: 5624141688 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)