BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0055.p (423 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF2000 [H] COG0499 S-adenosylhomocysteine hydrolase 483 e-136 NMA1275 [E] COG0548 Acetylglutamate kinase 473 e-133 STM0172 [V] COG1131 ABC-type multidrug transport system, ATPase ... 462 e-130 AGc4670 [T] COG0664 cAMP-binding proteins - catabolite gene acti... 258 1e-68 AGc4693 [R] COG4671 Predicted glycosyl transferase 258 1e-68 >AF2000 [H] COG0499 S-adenosylhomocysteine hydrolase Length = 432 Score = 483 bits (1242), Expect = e-136, Method: Compositional matrix adjust. Identities = 23/402 (5%), Positives = 67/402 (16%), Gaps = 1/402 (0%) Query: 19 EGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKVVQAGGAEVTI 78 +KI N E + + + ++ + Sbjct: 2 SKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLG 61 Query: 79 TGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALESKPDLIIDDGGDFATLL 138 G + A G + + I Sbjct: 62 KGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTENKSKFGANAILAVSLANAKA 121 Query: 139 HSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDNRYGTGQSA 198 + + T G + I N +++ Sbjct: 122 AAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHADNNVDIQEFMIQPVGAKTVKE 181 Query: 199 WDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVEAHMDGFHV 258 + +A G + + + G + Sbjct: 182 AIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGSNAEALAVIAEAVKAAGYELGKDI 241 Query: 259 MPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPELSDRSVSQR 318 ++ A + + + + + L + Sbjct: 242 TLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHFLEELTKQYPIVSIEDGLDESDWDGF 301 Query: 319 TVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKYVNDNYKTI 378 + + +++ L + + A + F + + Sbjct: 302 AYQTKVLGDKIQL-VGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAK 360 Query: 379 GVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSWN 420 V + + L + + S Sbjct: 361 DAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDR 402 >NMA1275 [E] COG0548 Acetylglutamate kinase Length = 252 Score = 473 bits (1217), Expect = e-133, Method: Compositional matrix adjust. Identities = 0/410 (0%), Positives = 0/410 (0%), Gaps = 4/410 (0%) Query: 11 VADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKVVQ 70 ++D + + H + R + +L R+ + G + L + A++ Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 71 AGGAEVTITGSNPLSTQDDVCAAL-VEDGITVFAKYNPSPEEFKALNIKALESKPDLIID 129 + I + A + + L + + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 130 DGGDFATLLHSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFD 189 + + + ++ G+ + + ++ Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 190 NRYGTGQSAWDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAV 249 Y + I+ + + + + L + E I Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 250 EAHMDGFHVMPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPE 309 Sbjct: 241 300 Query: 310 LSDRSVSQRTVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLK 369 Sbjct: 301 - 359 Query: 370 YVNDNYKTIGVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSW 419 Sbjct: 360 -- 407 >STM0172 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 308 Score = 462 bits (1189), Expect = e-130, Method: Compositional matrix adjust. Identities = 0/401 (0%), Positives = 0/401 (0%) Query: 20 GHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKVVQAGGAEVTIT 79 + + + +R + + T + Sbjct: 4 ALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGKSTTIGIISSLVNKTSGRVN 63 Query: 80 GSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALESKPDLIIDDGGDFATLLH 139 +D V A + +NP + + +A + L Sbjct: 64 VFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQAGYYGVEHKEAVLRSEKYLKQ 123 Query: 140 SERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDNRYGTGQSAW 199 + + + + + L + +G + Sbjct: 124 LDLWEKRHERARMLSGGMKRRLMIARALMHEPKLLILDEPTAGVDIELRRSMWGFLKDLN 183 Query: 200 DGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVEAHMDGFHVM 259 + ++ + +++++ + + + Sbjct: 184 AKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENTSMKNLLSKLKSETFILDLAPKSPLP 243 Query: 260 PMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPELSDRSVSQRT 319 + R T+ +G + + + N+ E S+ Sbjct: 244 KLTGYQYRLVDTSTLEVEVLREQGVNSVFSQLSEQGVQVLSMRNKANRLEELFVSLVHEK 303 Query: 320 VRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKYVNDNYKTIG 379 Sbjct: 304 363 Query: 380 VKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSWN 420 Sbjct: 364 404 >AGc4670 [T] COG0664 cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases Length = 297 Score = 258 bits (659), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 0/450 (0%), Positives = 0/450 (0%), Gaps = 41/450 (9%) Query: 6 KENSIVADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYL 65 + + ++ +P V ++ +P ++V + L Sbjct: 4 PICHLFSAINSSPFKIAPEKEQDLKTIVDDKKIIISVVSEPGFNIRVRKNESNNSHEIVL 63 Query: 66 AKVVQAGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALE---- 121 + E +E K L+ Sbjct: 64 TVASLEYIWAFSNFFWVFTQEYSKSQKNNDEHFDLTGKNRLKKSDELLKWARKNLQTTGC 123 Query: 122 SKPDLIIDDGGDFATLL----------------HSERPDLM------------------- 146 + Sbjct: 124 ESWPKKCPKPEAYLQGSEDSQVASEIFLCAIAWILHHEISHVVLQHPLVTTAFSTQEERE 183 Query: 147 ENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDNRYGTGQSAWDGIVRTT 206 + L+K+ + V + + T +A++ I Sbjct: 184 ADSHATKWILGNLYESAPELKKRALGIATAVLCIQSLEVENYFCLQNTHPAAYERIYSNI 243 Query: 207 NLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVEAHMDGFHVMPMLEAAK 266 Sbjct: 244 303 Query: 267 RGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPELSDRSVSQRTVRKNIEE 326 Sbjct: 304 -- 361 Query: 327 YQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKYVNDNYKTIGVKVENVP 386 Sbjct: 362 421 Query: 387 YDLDEQVARFKLESLGISIDSLTPAQVEYL 416 Sbjct: 422 451 >AGc4693 [R] COG4671 Predicted glycosyl transferase Length = 301 Score = 258 bits (659), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 0/450 (0%), Positives = 0/450 (0%), Gaps = 41/450 (9%) Query: 6 KENSIVADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYL 65 ++ + K + + + I + + + + +A Sbjct: 4 DKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDT 63 Query: 66 AKVVQAGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALE---- 121 + + + S+ ++ + K KA Sbjct: 64 PGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAV 123 Query: 122 SKPDLIIDDGGDFATLL----------------HSERPDLM------------------- 146 +K D + + L + Sbjct: 124 NKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPED 183 Query: 147 ENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDNRYGTGQSAWDGIVRTT 206 + IIR K ++ G V V Y Sbjct: 184 YITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQK 243 Query: 207 NLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVEAHMDGFHVMPMLEAAK 266 Sbjct: 244 303 Query: 267 RGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPELSDRSVSQRTVRKNIEE 326 Sbjct: 304 -- 361 Query: 327 YQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKYVNDNYKTIGVKVENVP 386 Sbjct: 362 421 Query: 387 YDLDEQVARFKLESLGISIDSLTPAQVEYL 416 Sbjct: 422 451 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,608,707 Number of extensions: 1323783 Number of successful extensions: 5543 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 5485 Number of HSP's successfully gapped: 63 Length of query: 423 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 314 Effective length of database: 25,807,025 Effective search space: 8103405850 Effective search space used: 8103405850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)