BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0051.p (432 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2573 [M] COG0768 Cell division protein FtsI/penicillin-binding... 261 2e-69 BS_pbpB [M] COG0768 Cell division protein FtsI/penicillin-bindin... 243 6e-64 BH2572 [M] COG0768 Cell division protein FtsI/penicillin-binding... 240 4e-63 BS_spoVD [M] COG0768 Cell division protein FtsI/penicillin-bindi... 221 2e-57 lin2145 [M] COG0768 Cell division protein FtsI/penicillin-bindin... 200 3e-51 >BH2573 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 723 Score = 261 bits (666), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 153/417 (36%), Positives = 232/417 (55%), Gaps = 41/417 (9%) Query: 4 LNGEYWQEKAAAQWAHSATIKAVRGEIEDRNGNVLASDVPAYTVVVNPEVIAEQGIGQEV 63 + G Q A A+W S + RG I DRNG+ LA +V +Y+V V Sbjct: 39 VKGYSLQAIAEARWTSSEVLHGKRGTIRDRNGDALAEEVTSYSVYA-------------V 85 Query: 64 IDGLH-ALLGKPVDELKKLVEAKDEKSGKYLK-------NREIRNEGWKINKELKDKVVA 115 +D H + P + L E + + L+ E+ + +E +DK+ Sbjct: 86 LDNSHQPAVEDPERTAQLLAEHINMSEERLLELLTRDVYQVELGAGARNLTQEQRDKIA- 144 Query: 116 FYEELGKKHDIRETGVGVVTEQKRYYPKDTLAAHILGYTDRD-GKAIMGLEKYFDKQLKG 174 D+ G+ E +RYYPK T A+H++GYTDRD G++ MGLE+ D+ L+G Sbjct: 145 ---------DLDLPGIYFTEEPRRYYPKHTYASHLIGYTDRDMGESRMGLERSLDEYLRG 195 Query: 175 TDGKLSYQSDGKGIKLPNSQDTYQPVVNGSNFKLTIDSTIQHYIEAAMQKAYDKYRPKSI 234 DG++ ++ DG+GI LP + +P G++ LT+DS IQ ++ M + ++Y+P+ I Sbjct: 196 EDGEIRFKKDGQGIPLPRPSEQIEPAKTGNDVYLTLDSNIQTALDQVMTQVEEEYKPERI 255 Query: 235 SVIAADPNTMEILGLANMPTFNPNKFWDPNQDAAGFYNHAIKSRYEPGSTFKIVTLAGAV 294 I ADP T +IL ++N PTFNPN++ + + N+A+ RYEPGST K+ TLA A+ Sbjct: 256 IAIVADPKTGQILAMSNRPTFNPNEY----EQITNYMNYAVSDRYEPGSTMKVFTLAAAL 311 Query: 295 EEKLFDPNAKFQSGSIRIKG-YSKALHDINR-VGWGTISFLEGVKRSSNVAFVKLGYEML 352 EE + + N ++QSG+ I+ + + D N+ GWGTIS+LE ++RSSNV F K+ E L Sbjct: 312 EEGVLNVNEQYQSGTYAIRNDTNPPIRDHNQGRGWGTISYLEAMQRSSNVGFSKIALEKL 371 Query: 353 GQERLLQYINDFGFNEKTGIDLPGETIGIVNPDPSIAAENATLAYGHGKVLVTPIQQ 409 G E+L +Y+++FGF E TGIDLP E + + A+ A+G G VTPIQQ Sbjct: 372 GPEKLYEYLDEFGFGEPTGIDLPNEAASVYA--KGALRDAASTAFGQGTA-VTPIQQ 425 >BS_pbpB [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 491 Score = 243 bits (619), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 27/415 (6%), Positives = 71/415 (17%), Gaps = 33/415 (7%) Query: 5 NGEYWQEKAAAQWAHSATIKAVRGEIEDRNGNVLASDVPAYTVVVNPEVIAEQGIGQEVI 64 A + + + +G+ + ++ A Sbjct: 43 ILADHPALEGAMAGITVRSAETGAVLYEHSGDTRMRPASSLKLLT----AAAALSVLGEN 98 Query: 65 DGLHAL-LGKPVDELKKLVEAKD----EKSGKYLKNREIRNEGWKINKELKDKVVAFYEE 119 + KKL + + E K + + Sbjct: 99 YSFTTEVRTDGTLKGKKLNGNLYLKGKGDPTLLPSDFDKMAEILKHS----------GVK 148 Query: 120 LGKKHDIRETGVGVVTEQKRYYPKDTLAAHILGYTDRDGKA-----IMGLEKYFDKQLKG 174 + K + I + P + + G + Sbjct: 149 VIKGNLIGDDTWHDDMRLSPDMPWSDEYTYYGAPISALTASPNEDYDAGTVIVEVTPNQK 208 Query: 175 TDGKLSYQSDGKGIKLPNSQDTYQPVVNGSNFKLTIDSTIQHYIEAAMQKAYDKYRPKSI 234 + + K + D + I D + K Sbjct: 209 EGEEPAVSVSPKTDYITIKNDAKTTAAGSEKDLTIEREHGTNTITIEGSVPVDANKTKEW 268 Query: 235 SVIAADPNTMEILGLANMPTFNPNKFWDPNQDAAGFYNHAIKSRYEPGSTFKIVTLAGAV 294 + L ++ + S ++ Sbjct: 269 ISVWEPAGYALDLFKQSLKKQGITV-----KGDIKTGEAPSSSDVLLSHRSMPLSKLFVP 323 Query: 295 EEKLFDPNAKFQSGSIRIKGYSKALHDINRVGWGTISFLEGVKRSSNVAFVKLGYEMLGQ 354 KL + K + S L S ++ G + Sbjct: 324 FMKLSNNGHAEVLVKEMGKVKKGEGSW-EKGLEVLNSTLPEFGVDSKSLVLRDGSGISHI 382 Query: 355 ERLLQYINDFGFNEKTGIDLPGETIGIVNPDPSIAAENATLAYGHGKVLVTPIQQ 409 + + + + + + + ++ Q Sbjct: 383 DAVSSDQLSQLLYDIQDQSWFSAYLNSLPVAGNP--DRMVGGTLRNRM-KGTPAQ 434 >BH2572 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 644 Score = 240 bits (612), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 159/416 (38%), Positives = 227/416 (54%), Gaps = 36/416 (8%) Query: 1 VQVLNGEYWQEKAAAQWAHSATIKAVRGEIEDRNGNVLASDVPAYTVVVNPEVIAEQGIG 60 VQ G++ +A W+ +A RGEI DRN VLA++V A +++V P + + Sbjct: 33 VQFALGDWLTGQAQDSWSRDVPFEAKRGEITDRNDVVLATNVSAPSILVVPRQVKDPA-- 90 Query: 61 QEVIDGLHALLGKPVDELKKLVEAKDEKSGKYLKNRE----IRNEGWKINKELKDKVVAF 116 + ++L +++ +K+ + + RE + EG KI+K+ ++V Sbjct: 91 ------------ETAEKLANVLQMDRQKAYELITKRESIVRLNPEGRKISKDKANEV--- 135 Query: 117 YEELGKKHDIRETGVGVVTEQKRYYPKDTLAAHILGYTDRDGKAIMGLEKYFDKQLKGTD 176 +R GV + + KR+YP + +H+LG+ D + + GLE Y+D+QLKG Sbjct: 136 -------RKLRLPGVYIAEDSKRHYPFGSYLSHVLGFAGIDNQGLTGLELYYDEQLKGEK 188 Query: 177 GKLSYQSDGKGIKLPNSQDTYQPVVNGSNFKLTIDSTIQHYIEAAMQKAYDKYRPKSISV 236 G +S+ SD KG +LPN D Y +NG N +LTIDS +Q IE + A Y P Sbjct: 189 GHVSFFSDAKGQRLPNLADEYTAPINGLNLRLTIDSRVQTIIERELDIAEATYSPDGAIA 248 Query: 237 IAADPNTMEILGLANMPTFNPNKFWDPNQDAAGFYNHAIKSRYEPGSTFKIVTLAGAVEE 296 IA +PNT EILG+++ P +NP F + + N I +YEPGSTFKI+TLA A+EE Sbjct: 249 IAMNPNTGEILGMSSRPHYNPENFREVPPEVYN-QNKPIWMQYEPGSTFKIITLAAALEE 307 Query: 297 KLFD-PNAKFQS-GSIRIKGYSKALHDINRVGWGTISFLEGVKRSSNVAFVKLGYEMLGQ 354 D N +F G I + G+ L + G G+ +FLE V+ S N FV LG E LG+ Sbjct: 308 NEVDLTNDRFHDPGFIEVAGHR--LRCWKKGGHGSQTFLEVVENSCNPGFVVLG-ERLGK 364 Query: 355 ERLLQYINDFGFNEKTGIDLPGETIGIV-NPDPSIAAENATLAYGHGKVLVTPIQQ 409 +RL YI FGF +KTGIDL GE GI+ N D E AT A+G G V VTPIQQ Sbjct: 365 DRLFDYIEAFGFGQKTGIDLQGEAKGIIFNRDRIGPLEQATTAFGQG-VSVTPIQQ 419 >BS_spoVD [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 645 Score = 221 bits (562), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 152/422 (36%), Positives = 221/422 (52%), Gaps = 48/422 (11%) Query: 1 VQVLNGEYWQEKAAAQWAHSATIKAVRGEIEDRNGNVLASDVPAYTVVVNPEVIAEQGIG 60 VQ + GE A W+ + + RGEI DRNG LA++ A TV V P + Sbjct: 33 VQFVMGEKLTSLAKDSWSRNLPFEPERGEILDRNGVKLATNKSAPTVFVVPRQVQNPM-- 90 Query: 61 QEVIDGLHALLGKPVDELKKLVEAKDEKSGKYLKNR----EIRNEGWKINKELKDKVVAF 116 K +L ++ +EK K++ + +I EG KI+ E ++ A Sbjct: 91 ------------KTSKQLAAVLNMSEEKVYKHVTKKASIEKITPEGRKISNEKAKEIKAL 138 Query: 117 YEELGKKHDIRETGVGVVTEQKRYYPKDTLAAHILGYTDRDGKAIMGLEKYFDKQLKGTD 176 D++ GV V + R+YP + +H+LG+ D + ++GLE Y+D LKG Sbjct: 139 --------DLK--GVYVAEDSIRHYPFGSFLSHVLGFAGIDNQGLLGLEAYYDDDLKGEK 188 Query: 177 GKLSYQSDGKGIKLPNSQDTYQPVVNGSNFKLTIDSTIQHYIEAAMQKAYDKYRPKSISV 236 G + + +D KG K+P+ D Y P +G + KLT+DS +Q +E + A KY P + Sbjct: 189 GSVKFYTDAKGKKMPDEADDYTPPKDGLDMKLTVDSKVQTIMERELDNAEAKYHPDGMIA 248 Query: 237 IAADPNTMEILGLANMPTFNPNKFW--DPNQDAAGFYNHAIK--SRYEPGSTFKIVTLAG 292 +A +P EILG+++ P F+P + DP+ YN + S YEPGSTFKI+TLA Sbjct: 249 VAMNPKNGEILGMSSRPDFDPADYQSVDPS-----VYNRNLPVWSTYEPGSTFKIITLAA 303 Query: 293 AVEEKLFDPNAKFQSGSIRIKGYSKA----LHDINRVGWGTISFLEGVKRSSNVAFVKLG 348 A+EE+ + KG+++ L R G G ++LE V+ S N FV+LG Sbjct: 304 ALEEQ----KVNLKRDQFYDKGHAEVDGARLRCWKRGGHGLQTYLEVVQNSCNPGFVELG 359 Query: 349 YEMLGQERLLQYINDFGFNEKTGIDLPGETIGIVNPDPSIA-AENATLAYGHGKVLVTPI 407 E LG+E+L +YI DFGF +KTGIDL GE GI+ P + E AT A+G G V VTPI Sbjct: 360 -ERLGKEKLFKYIKDFGFGQKTGIDLQGEGTGILFPLERVGPVEQATTAFGQG-VSVTPI 417 Query: 408 QQ 409 QQ Sbjct: 418 QQ 419 >lin2145 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 331 Score = 200 bits (509), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 0/416 (0%), Positives = 0/416 (0%), Gaps = 38/416 (9%) Query: 5 NGEYWQEKAAAQWAHSATIKAVRGEIEDRNGNVLASDVPAYTV--VVNPEVIAEQGIGQE 62 Q A + + + A Q Sbjct: 40 LMTKVTSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQL 99 Query: 63 VID--GLHALLGK--PVDELKKLVEAKDEKSGKYLKNREIRNEGWKINKELKDKVVAFYE 118 + L V + + + + V Sbjct: 100 LAQLGEYLRLGNNRQAVTD-YIRHNFGQTP-LNQ--LSPEQLKTILTLLQEGKMV----- 150 Query: 119 ELGKKHDIRETGVGVVTEQKRYYPKDTLAAHILGY---------TDRDGKAIMGLEKYFD 169 + Sbjct: 151 ---IPQPQ--QREATDRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSML--ELSGVKDG 203 Query: 170 KQLKGTDGKLSYQSDGKGIKLPNSQDTYQPVVNGSNFKLTIDSTIQHYIEAAMQKAYDKY 229 + + L + + + S + QK Sbjct: 204 ELIPAKLFNHLVTWLQARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSA 263 Query: 230 RPKSISVIAADPNTMEILGLANMPTFNPNKFWDPNQDAAGFY-NHAIKSRYEPGSTFKIV 288 + S A D + + I+ Sbjct: 264 QSSLSSAQAEDILNQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAII 323 Query: 289 TLAGAVEEKLFDPNAKFQSGSIRIKGYSKALHDINR-VGWGTISFLEGVKRSSNVAFVKL 347 Sbjct: 324 -- 381 Query: 348 GYEMLGQERLLQYINDFGFNEKTGIDLPGETIGIVNPDPSIAAENATLAYGHGKVL 403 Sbjct: 382 --- 434 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,126,737 Number of extensions: 1518508 Number of successful extensions: 3082 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 2830 Number of HSP's successfully gapped: 102 Length of query: 432 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 323 Effective length of database: 25,807,025 Effective search space: 8335669075 Effective search space used: 8335669075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)