BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0050.p (269 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2573 [M] COG0768 Cell division protein FtsI/penicillin-binding... 114 1e-25 BH2572 [M] COG0768 Cell division protein FtsI/penicillin-binding... 111 1e-24 CAC2130 [M] COG0768 Cell division protein FtsI/penicillin-bindin... 108 9e-24 BS_spoVD [M] COG0768 Cell division protein FtsI/penicillin-bindi... 105 9e-23 BS_pbpB [M] COG0768 Cell division protein FtsI/penicillin-bindin... 97 3e-20 >BH2573 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 723 Score = 114 bits (286), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 85/247 (34%), Positives = 127/247 (51%), Gaps = 19/247 (7%) Query: 1 VVRQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTD-----Y 55 VV + ISKE+A+E LE VV GTGR Y+EG+ VAGKTGTA +VR D Sbjct: 463 VVGEPISKETAKEVRDILETVVTSS-SGTGRPFYLEGFDVAGKTGTA-QVRNPDGPGYLN 520 Query: 56 DRNKVLSSFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVP 115 K + SFIG AP +DP + V V +D P VA +F I++ +L Y+ + Sbjct: 521 GHGKNIFSFIGMAPKDDPSVIVYVAVDRPSLNTNQVGSEPVAK-IFNTIMNHSLQYLNIS 579 Query: 116 KASTETADSTSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYP 175 + E D + + D G++ ++AR+ L G +GEG TV Q P Sbjct: 580 PSVEELKDEEEEGYELA--------DFIGESARSAREELEQAGMKVYVLGEGDTVEGQQP 631 Query: 176 EKGTPLASGQRIYLLSQQGDKPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQAQG 235 G L G+R+ +L + + +P + G SLRD L+V ++L V + + G+G+V Q Sbjct: 632 YSGHKLLEGERV-ILRTESESYTLPSMIGWSLRDVLKVANVLDVNVNLFGQGFVV--GQS 688 Query: 236 LKNGKTL 242 ++ G T+ Sbjct: 689 IEEGDTI 695 >BH2572 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 644 Score = 111 bits (277), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 74/195 (37%), Positives = 104/195 (53%), Gaps = 11/195 (5%) Query: 3 RQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTDYDRNKVLS 62 RQ+IS+E+++E LE VVA +GTG+ A+++GYRV GKTGTA K + Y N + Sbjct: 459 RQIISEETSKEVRYALESVVA---RGTGQGAFVDGYRVGGKTGTAQKAKDGRYLENNHIV 515 Query: 63 SFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTETA 122 SFIG+AP +DP+I V V ID PK VAAP+ +I+ +L +G+ K + Sbjct: 516 SFIGFAPADDPQIVVYVAIDNPK--DTVQFGGVVAAPIVGKIIGDSLQALGIEKRENQ-- 571 Query: 123 DSTSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGTPLA 182 K K PDL G+T + + + D A G+G VV+Q PE GT +A Sbjct: 572 --IEKERKWNDEPLVEVPDLVGRTKRDLHESYYELKID--ADGKGDVVVAQAPEPGTKVA 627 Query: 183 SGQRIYLLSQQGDKP 197 G I L +P Sbjct: 628 VGSTIRLYMDDDTEP 642 >CAC2130 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 729 Score = 108 bits (270), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 80/245 (32%), Positives = 128/245 (52%), Gaps = 29/245 (11%) Query: 17 YLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVR--GTDYDRNKVLSSFIGYAPVNDPK 74 YLE+VV++ G G+ A+I+GY + GKTGTA K G Y+ K ++SF+G AP +DPK Sbjct: 494 YLEKVVSE---GGGKKAFIDGYHIGGKTGTAQKPNPNGGGYEHGKYIASFVGMAPASDPK 550 Query: 75 IAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTETADSTSKTVKAPAP 134 I V+V IDEP ++APV Q++ + Y+G+ K T ++D+ +K Sbjct: 551 ITVMVSIDEPDPSNYYAGQ--ISAPVGQKVFNDIFNYLGI-KPDTSSSDAAKSMLKDVV- 606 Query: 135 AQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGTPLASGQRIYLLSQQG 194 P++ G + A + L + D + G G V + P G + G ++ L + G Sbjct: 607 ----IPNIRGMKKEDAIKTLKNLNIDADVDGNGEYVSNTTPIPGITVKEGTKVVLYT--G 660 Query: 195 DKP-------VIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQAQGLKNGK-----TL 242 D P ++P+L G S ++A E L L + + G+G V ++Q + GK T+ Sbjct: 661 DSPNYNNNDVIVPNLTGYSKKEAEETLKSLGLDFKISGDGVV--ESQSIDAGKQVNKGTV 718 Query: 243 VTLKL 247 +TLKL Sbjct: 719 LTLKL 723 >BS_spoVD [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 645 Score = 105 bits (261), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 70/196 (35%), Positives = 103/196 (52%), Gaps = 22/196 (11%) Query: 3 RQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTDYDRNKVLS 62 +QVIS+E++++ LE VVA+ GTGR+A++EGYRV GKTGTA KV+ Y N + Sbjct: 459 KQVISEETSKQIRYALESVVAE---GTGRNAFVEGYRVGGKTGTAQKVKDGKYLENNHIV 515 Query: 63 SFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTETA 122 SFIG+AP +DP + V V +D PK P+ I+ +LP +GV + Sbjct: 516 SFIGFAPADDPSLVVYVAVDNPKGAFSLAER---WQPIVGNIMRDSLPELGVKPRKNQIE 572 Query: 123 D----STSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKG 178 + +KT++ P S DL LL++ D A G+G+ +V Q P G Sbjct: 573 KKYQWNDTKTIEVPNVVGMSVSDL--------ESLLVNLNVD--ASGKGSKIVKQSPAAG 622 Query: 179 TPLASGQ--RIYLLSQ 192 T + G R+YL + Sbjct: 623 TKVKEGSKIRVYLTEE 638 >BS_pbpB [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 491 Score = 97.1 bits (240), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 0/251 (0%), Positives = 0/251 (0%), Gaps = 19/251 (7%) Query: 6 ISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTA-IKVRGTDYD-RNKVLSS 63 Sbjct: 477 - 535 Query: 64 FIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTETAD 123 Sbjct: 536 --- 592 Query: 124 STSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGTPLAS 183 Sbjct: 593 ----- 647 Query: 184 GQRIYLLSQQGDKPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQA----QGLKNG 239 Sbjct: 648 --- 704 Query: 240 KTLVTLKL-NP 249 Sbjct: 705 715 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.131 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,989,427 Number of extensions: 702597 Number of successful extensions: 1645 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 1611 Number of HSP's successfully gapped: 42 Length of query: 269 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 164 Effective length of database: 26,268,361 Effective search space: 4308011204 Effective search space used: 4308011204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 115 (48.9 bits)