BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0048.p (497 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2129 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase 384 e-106 all1663 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase 318 1e-86 slr0528 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase 317 3e-86 CAC2819 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase 308 1e-83 Rv2158c [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase 282 1e-75 >CAC2129 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase Length = 211 Score = 384 bits (985), Expect = e-106, Method: Compositional matrix adjust. Identities = 0/497 (0%), Positives = 0/497 (0%), Gaps = 24/497 (4%) Query: 3 MKVNEFASCLAASRLYGDKETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANG 62 M + L R T + + L +V+ AN Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 63 ASALVCERKLDLDLPQ---IVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXY 119 +A++ ++ I + + + + + + Sbjct: 61 PAAMLE-LGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGR 119 Query: 120 LIERIMQDQNIKTGLIGTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVS 179 ++ + I + + +++ L +V + Sbjct: 120 KTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWL 179 Query: 180 SHALQQGRVKGTDYRTAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEERKY 239 Sbjct: 180 ----------- 228 Query: 240 AVLNADDEASAYFAAQTAAEVITYGIDSPANVRASQISITSKGTFFHVDTFKG-EADISL 298 Sbjct: 229 - 287 Query: 299 RMVGKFNVYNALAAITAALLEDVPLEEIKASLESVAGVAGRVESV--NEGQDFAVIVDYA 356 Sbjct: 288 347 Query: 357 HTPDGLENVLRAVCEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTE 416 Sbjct: 348 407 Query: 417 DPQLILKDIEAGLQEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIG 476 Sbjct: 408 ----- 462 Query: 477 GVVHDFDDRVVAKEVIR 493 Sbjct: 463 479 >all1663 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase Length = 496 Score = 318 bits (815), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 201/484 (41%), Positives = 277/484 (57%), Gaps = 22/484 (4%) Query: 22 ETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANGASAL-VCERKLDLDLPQ-- 78 + E+ G++ +S GDLFI +PG VDG +F P A A+GA A V + ++ + P Sbjct: 22 DAEVKGIKTNSHACGAGDLFIGMPGTRVDGGEFWPSAIASGAIAAIVSPQAVEKNPPHDE 81 Query: 79 ---IVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIERIMQDQNIKTGLI 135 I ++ A A ++ AF+G P +++++ +LIE + + T L+ Sbjct: 82 AVVISSNNMTKACAAIAAAFYGYPGQKLKLVGVTGTNGKTTTTHLIEFFLTKAKLSTALM 141 Query: 136 GTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHALQQGRVKGTDYRT 195 GT+ R+ G A+ TT ++ELQ+ L+ V G + VMEVSSHAL QGRV G + Sbjct: 142 GTLYTRWPGFEQTATHTTPFAVELQQQLAQAVNAGCEFGVMEVSSHALAQGRVLGCPFEV 201 Query: 196 AIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEERKYAVLNADDE-ASAYFAA 254 +F+NLTQDHLDYH ME+Y A K L FS + + A++NADD A Sbjct: 202 GVFSNLTQDHLDYHSDMEDYFAAKALLFSP--------EYLKGRAIINADDTYGQRLIKA 253 Query: 255 QTAAEVITYGI-DSPANVRASQISITSKGTFFHVDTFKGEADISLRMVGKFNVYNALAAI 313 + +V +Y + DS A++ S +S G + T +G +VG++N+ N LAA+ Sbjct: 254 LSPEKVWSYSVNDSSADLWMSDLSYEPNGVTGTIHTPEGNVSFRSPLVGQYNLENLLAAV 313 Query: 314 TAALLEDVPLEEIKASLESVAGVAGRVESV--NEGQDFAVIVDYAHTPDGLENVLRAVCE 371 A L + L+ I ++ GV GR+E V N QD +VIVDYAHTPD LEN+L+A Sbjct: 314 GAVLHLGLNLQLIANAIPEFPGVPGRMERVQINPDQDISVIVDYAHTPDSLENLLKAARP 373 Query: 372 FAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLILKDIEAGLQE 431 F GR++ VFGCGGDRD TKRP MGKI A+ +D FVTSDNPRTEDP IL DI AG+ Sbjct: 374 FIPGRMICVFGCGGDRDRTKRPKMGKIVAELADLAFVTSDNPRTEDPDRILDDILAGI-P 432 Query: 432 DGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHDFDDRVVAKEV 491 D V + +I DR AIR AI A P D VL+AGKGHE YQ++G FDDR A+ Sbjct: 433 DTV---QPTVIGDRAIAIRTAILQAQPGDGVLLAGKGHEDYQILGTEKIHFDDREHARAA 489 Query: 492 IRGR 495 + R Sbjct: 490 LTER 493 >slr0528 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase Length = 211 Score = 317 bits (812), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 0/483 (0%), Positives = 0/483 (0%), Gaps = 32/483 (6%) Query: 25 ITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANGA-SALVCERKLDLDLPQ----- 78 L A + V L Sbjct: 35 AVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCR 94 Query: 79 IVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIERIMQDQNIKTGLIGTI 138 I+++ + A P + ++ + +T Sbjct: 95 ILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGK 154 Query: 139 QMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHALQQGRVKGTDYRTAIF 198 Sbjct: 155 214 Query: 199 TNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEE--RKYAVLNADDEASAYFAAQT 256 Sbjct: 215 ---------- 264 Query: 257 AA-EVITYGI-DSPANVRASQISITS---KGTFFHVDTFKGEADISLRMVGKFNVYNALA 311 Sbjct: 265 --- 321 Query: 312 AITAALLEDVPLEEIKASLESVAGVAGRVESVN--EGQDFAVIVDYAHTPDGLENVLRAV 369 Sbjct: 322 381 Query: 370 CEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLILKDIEAGL 429 Sbjct: 382 441 Query: 430 QEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHDFDDRVVAK 489 Sbjct: 442 ---- 497 Query: 490 EVI 492 Sbjct: 498 500 >CAC2819 [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase Length = 483 Score = 308 bits (789), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 176/497 (35%), Positives = 266/497 (53%), Gaps = 22/497 (4%) Query: 3 MKVNEFASCLAASRLYGDKETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANG 62 MK+ + + + GD I G+ +S++VK +F+ + G DGHDF +AA NG Sbjct: 1 MKLKQLLQNIDYDLIKGDININIEGISYNSKKVKQNYVFVSIKGARFDGHDFIKEAAQNG 60 Query: 63 ASALVCERKLDLDLPQ---IVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXY 119 A+A++ + + + V + + A+A L N F+ PS + ++ + Sbjct: 61 AAAVIIDEDFHDSIENTTLVKVKNSKVALACLCNLFYEEPSRELNLVGVTGTNGKTTVIH 120 Query: 120 LIERIMQDQNIKTGLIGTIQMRYDGQTFPA---SGTTQESLELQRTLSDMVQKGVQCCVM 176 I +++ TG IGT+ + + TT E+LEL + L D + KG + VM Sbjct: 121 YIRDMLEAFGNSTGTIGTLGYELKDKEINVEKINPTTPEALELNQILRDFIDKGAKNAVM 180 Query: 177 EVSSHALQQGRVKGTDYRTAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEE 236 EV+S AL + RV ++ +FTNL+QDHLD H T+E Y+ K F + P Sbjct: 181 EVTSSALMKHRVDYCSFKVGVFTNLSQDHLDEHGTIENYKNEKIKLFK-----MCPV--- 232 Query: 237 RKYAVLNADDEASAYFAAQTAAEVITYGIDSPANVRASQISITSKGTFFHVDTFKG-EAD 295 V+N DD + + E TYG+ + A++ AS + F+V +KG + Sbjct: 233 ---GVINLDDNIAGEIIEKGTCEFFTYGVLTNADLTASDMVYKPNSVSFNVH-YKGISSK 288 Query: 296 ISLRMVGKFNVYNALAAITAALLEDVPLEEIKASLESVAGVAGRVESVNEGQDFAVIVDY 355 + + + G+F VYN LAAI + L + E I + S+ V GR+E++N + +I+DY Sbjct: 289 VKVNVPGEFTVYNVLAAIGSLLCLGIDFESILKLVSSIKPVLGRLETINNPLNKNIIIDY 348 Query: 356 AHTPDGLENVLRAVCEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRT 415 AHTPD LE +L E GR++TVFGCGGDRD +KR LMG SD+ +TSDNPR Sbjct: 349 AHTPDSLEKLLIMAREMTNGRIITVFGCGGDRDKSKRGLMGMAVGILSDYCIITSDNPRH 408 Query: 416 EDPQLILKDIEAGLQEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLI 475 E P+ I++DIE G+Q Y+ I DRR AI++ IE+ D+++IAGKGHE YQ+I Sbjct: 409 EKPEDIIRDIERGMQ---TINSSYEKITDRRCAIKRGIEILKEEDILIIAGKGHEDYQII 465 Query: 476 GGVVHDFDDRVVAKEVI 492 G FDDR V E++ Sbjct: 466 GDNKVHFDDREVVNELL 482 >Rv2158c [M] COG0769 UDP-N-acetylmuramyl tripeptide synthase Length = 535 Score = 282 bits (721), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 189/477 (39%), Positives = 255/477 (53%), Gaps = 23/477 (4%) Query: 21 KETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANGASAL------VCERKLDL 74 ++ +TG+ ++ V PGDLF L G T G A A GA A+ V E Sbjct: 58 EDRTVTGVTLRAQDVSPGDLFAALTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRA 117 Query: 75 DLPQIVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIERIMQDQNIKTGL 134 +P +V R + L+ +G PS R+ +I YL+E ++ GL Sbjct: 118 AVPVLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTTTYLVEAGLRAAGRVAGL 177 Query: 135 IGTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHALQQGRVKGTDYR 194 IGTI +R G P++ TT E+ LQ L+ MV++GV VMEVSSHAL GRV GT + Sbjct: 178 IGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFA 237 Query: 195 TAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEER-KYAVLNADDEASAYFA 253 FTNL++DHLD+H +M +Y K F P R + AV+ DD+A A Sbjct: 238 VGAFTNLSRDHLDFHPSMADYFEAKASLF-------DPDSALRARTAVVCIDDDAGRAMA 290 Query: 254 AQTAAEVITYGIDSPANVRASQISITSKG--TFFHVDTFKGEADISLRMVGKFNVYNALA 311 A+ A + D PA+ RA+ ++ T G F +D I +R+ G++NV N L Sbjct: 291 ARAADAITVSAADRPAHWRATDVAPTDAGGQQFTAIDPAGVGHHIGIRLPGRYNVANCLV 350 Query: 312 AITAALLEDVPL--EEIKASLESVAGVAGRVESVNEGQDFAVIVDYAHTPDGLENVLRAV 369 A+ A+L+ V + E+ L + V GR+E ++ GQ F +VDYAH P+ L +VL + Sbjct: 351 AL--AILDTVGVSPEQAVPGLREIR-VPGRLEQIDRGQGFLALVDYAHKPEALRSVLTTL 407 Query: 370 CEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLILKDIEAGL 429 R+ VFG GGDRD KR MG+IAA+ +D V VT DNPR EDP I ++I AG Sbjct: 408 AH-PDRRLAVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGA 466 Query: 430 QEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHDFDDRV 486 E G + I DRR+AIR A+ A P DVVLIAGKGHET Q GG V FDDRV Sbjct: 467 AEVG-GDAQVVEIADRRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRV 522 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,923,882 Number of extensions: 1361335 Number of successful extensions: 3436 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 3366 Number of HSP's successfully gapped: 29 Length of query: 497 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 386 Effective length of database: 25,576,357 Effective search space: 9872473802 Effective search space used: 9872473802 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)