BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0045.p (307 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mraY [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotran... 285 6e-77 lin2143 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotran... 243 3e-64 BH2568 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotrans... 221 1e-57 SA1025 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotrans... 218 1e-56 CAC2127 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotran... 198 8e-51 >BS_mraY [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 285 bits (729), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 14/301 (4%), Positives = 48/301 (15%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M ++ + T ++ + + F ++ + +M Sbjct: 1 MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQTRAVM 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 + + + ++ + + + K + G + L + Sbjct: 61 LQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRGLQKE 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 + S + + A +N Sbjct: 121 TEKSFARWHNDLEHQATWLESNQLSDFLTAPV-QGSQNAFDVNFEAWQLEINHVLEAASA 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 S + + L + Sbjct: 180 QSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIAV 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 + L + + + Q + Sbjct: 240 YGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQAASLA 299 Query: 297 W 297 Sbjct: 300 Q 300 >lin2143 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 243 bits (620), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 15/302 (4%), Positives = 51/302 (16%), Gaps = 6/302 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M ++ + T ++ + + F ++ + +M Sbjct: 1 MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQTRAVM 60 Query: 61 VAFTLSFLKFSVVNSDF----YVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 + + + K + + + + + K + G + L + Sbjct: 61 LQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRGLQKE 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 + S + + A +N Sbjct: 121 TEKSFARWHNDLEHQATWLESNQLSDFLTAP-VQGSQNAFDVNFEAWQLEINHVLEAASA 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 S + + L + Sbjct: 180 QSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIAV 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFK-TRGKRIFRMSPIHHHFELGGWS 295 + L + + + Q + + Sbjct: 240 YGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQAASLA 299 Query: 296 EW 297 + Sbjct: 300 QT 301 >BH2568 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 221 bits (562), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 16/222 (7%), Positives = 30/222 (13%), Gaps = 1/222 (0%) Query: 76 DFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQLLVGAVMCGLLINAGHNTG 135 + + L LI I + L Q+ L Sbjct: 2 FNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQTRAVML 61 Query: 136 ISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGLLSGXXXXXXXXXXXXXXXX 195 + + + YP I + T + T S Sbjct: 62 QASTALNKAGTLT-ALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRGLQKE 120 Query: 196 TSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXXXXXXXXXVTKSELLFVVIG 255 T L Sbjct: 121 TEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAASAQ 180 Query: 256 GVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSEW 297 ++ ++V + Sbjct: 181 SQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIG 222 >SA1025 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 218 bits (555), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 14/304 (4%), Positives = 43/304 (14%), Gaps = 13/304 (4%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M ++ + T ++ + + RDD + + + + Sbjct: 1 MFNRIRISTTLFLILIL----CGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQT 56 Query: 61 VAFTLSFLK---FSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQL 117 A L + + +S + Sbjct: 57 RAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRG 116 Query: 118 LVGAVMCGLLINAGH----NTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGV 173 L + F + + + Sbjct: 117 LQ-KETEKSFARWHNDLEHQATWLESNQLSDFLTA-PVQGSQNAFDVNFEAWQLEINHVL 174 Query: 174 DGLLSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFX 233 + + + + G+ Sbjct: 175 EAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLA 234 Query: 234 XXXXXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGG 293 +T + + V I + Sbjct: 235 RPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQ 294 Query: 294 WSEW 297 + Sbjct: 295 AASL 298 >CAC2127 [M] COG0472 UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase Length = 533 Score = 198 bits (504), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 13/294 (4%), Positives = 43/294 (14%), Gaps = 4/294 (1%) Query: 5 LLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIMVAFT 64 + F++ ++ L I + + + V Sbjct: 3 NRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVEQSNQQRAALAQTRAVMLQ 62 Query: 65 LSFLKF-SVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQLLVGAVM 123 S + + +S + L Sbjct: 63 ASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRGLQKETE 122 Query: 124 CGL-LINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGLLSGXXX 182 + + V + L + Sbjct: 123 KSFARWHNDLEH--QATWLESNQLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAASAQ 180 Query: 183 XXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXXXXXXXX 242 + ++ D + Sbjct: 181 SQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIAVY 240 Query: 243 XVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 + +F + + +V + I + ++ + Sbjct: 241 GRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIGIAEIVAGNNDLSSRTEQQ 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.146 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,533,834 Number of extensions: 672534 Number of successful extensions: 1947 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 1860 Number of HSP's successfully gapped: 73 Length of query: 307 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 201 Effective length of database: 26,153,027 Effective search space: 5256758427 Effective search space used: 5256758427 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)