BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0031.p (285 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yfiH [G] COG1082 Sugar phosphate isomerases/epimerases 95 1e-19 YPO3276 [S] COG1496 Uncharacterized conserved protein 93 4e-19 PA4543 [S] COG1496 Uncharacterized conserved protein 91 1e-18 PA1469 [S] COG1496 Uncharacterized conserved protein 78 2e-14 YPO1747 [S] COG1496 Uncharacterized conserved protein 69 7e-12 >BS_yfiH [G] COG1082 Sugar phosphate isomerases/epimerases Length = 313 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 8/256 (3%), Positives = 31/256 (12%), Gaps = 31/256 (12%) Query: 26 ITAGFTGRQGGIGKAPYDSFNCAFHVGDEPTDVLGNRTLLAESLGFTLQD-WTCGEQTHG 84 + D L + + L+ Sbjct: 15 FEDMLNFAAKLGIDTLEMTTGGWSPAPHLNLDELLQSSEKRKEFSQALEKRNM--TLCAL 72 Query: 85 NDIAVVKADDRGRGSLDRASAFQGTDGLLTNVPGVLLTSFYADCVPLYFYDPLKKVVGLA 144 N + + + + + + Sbjct: 73 NCSGNPLDPGE---------LGKSHRDITDKTMELAGLLGVKKVIMMSGLPAGGPDDKVP 123 Query: 145 HAGWKGTVAEIAAAMVARMETGYGSRPQDILAGIGPSIGDCCYEVDDYVMDHIRQLEDGL 204 + ++ V + Y I V +++ + Sbjct: 124 NWITYT----VSWPPVLKDILNYQWEDVAIPYWKKLVKKAEACGVGKIALENFSSQL--V 177 Query: 205 KAAAGSGPSQDLYRTSEADDRKSMLNLKEMNRRIMIKAGILPTHIECTSWCTSCNQDLFF 264 ++ + + + Sbjct: 178 YNPETLFRLRNAV----------GPMVGLNLDPSHLLWMG--ADPIIAARELGSAIHHVH 225 Query: 265 SYRKENGVTGRMTSWI 280 Sbjct: 226 GKDVRI-ERYLSAVNG 240 >YPO3276 [S] COG1496 Uncharacterized conserved protein Length = 243 Score = 93.2 bits (230), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 76/258 (29%), Positives = 116/258 (44%), Gaps = 31/258 (12%) Query: 24 AEITAGFTGRQGGIGKAPYDSFNCAFHVGDEPTDVLGNRTLLAESLGF-TLQDWTCGEQT 82 A + A T R GG+ +PYDS N HVGD T V NR LL E + W +Q Sbjct: 13 ARVRACSTTRHGGVSVSPYDSLNLGTHVGDVATHVDANRQLLREQACLPQMPVWL--DQV 70 Query: 83 HGNDIAVVKADDRGRGSLDRASAFQGTDGLLTNVPGVLLTSFYADCVPLYFYDPLKKVVG 142 HG + ++ R+ + D + + V G + ADC+P+ F V Sbjct: 71 HGTRVLTLEG---------RSVSNTQADAVYSRVVGQVCAVMTADCLPVLFCSLAGDEVA 121 Query: 143 LAHAGWKGTVAEIAAAMVARMETGYGSRPQDILAGIGPSIGDCCYEVDDYVMDHIRQLED 202 AHAGW+G + + VA+ + + P ++A +GP+IG +EV + V ++ Sbjct: 122 AAHAGWRGLCSGVLEHTVAQ----FNAAPSSLMAWLGPAIGPQQFEVGEDVKQAFISIDA 177 Query: 203 GLKAAAGSGPSQDLYRTSEADDRKSMLNLKEMNRRIMIKAGILPTHIECTSWCTSCNQDL 262 K+A P Y + ++ + R + AGI I + CT + Sbjct: 178 --KSALAFTPFGSKY----------LADIYLLARLRLQAAGI--QAIYGGNRCTVTEKQQ 223 Query: 263 FFSYRKENGVTGRMTSWI 280 FFSYR++ G+TGRM S I Sbjct: 224 FFSYRRD-GITGRMASLI 240 >PA4543 [S] COG1496 Uncharacterized conserved protein Length = 242 Score = 91.3 bits (225), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 117/261 (44%), Gaps = 31/261 (11%) Query: 24 AEITAGFTGRQGGIGKAPYDSFNCAFHVGDEPTDVLGNRTLLAESLGFTLQDWTCGEQTH 83 A + A T R GG+ +AP+DS N HV D+P V NR L E L W +Q H Sbjct: 13 ARVRACVTTRSGGVSQAPFDSLNLGAHVDDDPRAVEENRRRLTERLE-CRPSWL--DQVH 69 Query: 84 GNDIAVVKADDRGRGSLDRASAFQGTDGLLTNVPGVLLTSFYADCVPLYFYDPLKKVVGL 143 G + VV+AD S D + +PGV T ADC+P F D V Sbjct: 70 G--VTVVEADP---------SRVLRADASWSAMPGVACTIMTADCLPALFCDRSGTRVAA 118 Query: 144 AHAGWKGTVAEIAAAMVARMETGYGSRPQDILAGIGPSIGDCCYEVDDYVMDHIRQLEDG 203 AHAGW+G A + A V + G ++L +GP+IG +EV ++ D Sbjct: 119 AHAGWRGLAAGVLEATVDSL----GVPGDELLVWLGPAIGPRAFEVGG-------EVRDA 167 Query: 204 LKAAAGSGPSQDLYRTSEADDRKSMLNLKEMNRRIMIKAGILPTHIECTSWCTSCNQDLF 263 AA S + A+ + M ++ + R + G+ T + +CT + F Sbjct: 168 FVAAHAEARSAFV---PSANPGRFMADIYRLARIRLGAHGV--TAVHGGGFCTFSDTARF 222 Query: 264 FSYRKENGVTGRMTSWIGIKE 284 +SYR+ + TGR S + +++ Sbjct: 223 YSYRRSSR-TGRFASLVWLQD 242 >PA1469 [S] COG1496 Uncharacterized conserved protein Length = 240 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 59/200 (29%), Positives = 85/200 (42%), Gaps = 20/200 (10%) Query: 81 QTHGNDIAVVKADDRGRGSLDRASAFQGTDGLLTNVPGVLLTSFYADCVPLYFYDPLKKV 140 Q HG +I D R R +A DG+ + P + + ADC+P+ +V Sbjct: 34 QVHGAEIV----DARSLSESGRHAA----DGVFSEGP-LAVAVVTADCLPILMSSRDGRV 84 Query: 141 VGLAHAGWKGTVAEIAAAMVARMETGYGSRPQDILAGIGPSIGDCCYEVDDYVMDHIRQL 200 V H GW+G VA I A VAR G DI IGP I CCYEV ++ ++ Sbjct: 85 VAALHGGWQGLVAGIVGAAVARF-NARGVASADIRVAIGPGIKACCYEVSAGFIEALQVG 143 Query: 201 EDGLKAAAGSGPSQDLYRTSEAD----------DRKSMLNLKEMNRRIMIKAGILPTHIE 250 L A SQ ++ + L+L R++ AG+ P IE Sbjct: 144 HGRLWEGAAPPWSQRRPLPERPPRFAPPEPRQAEQGAWLDLVAFCRQLFQAAGVGPAQIE 203 Query: 251 CTSWCTSCNQDLFFSYRKEN 270 ++ CT C+ D F S+R+ Sbjct: 204 ASAVCTYCSGDAFASHRRRQ 223 >YPO1747 [S] COG1496 Uncharacterized conserved protein Length = 251 Score = 69.3 bits (168), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 44/138 (31%), Positives = 68/138 (49%), Gaps = 12/138 (8%) Query: 59 LGNRT-LLAESLGFTLQDWTCGEQTHGNDIAVVKADDRGRGSLDRASAFQGTDGLLTNVP 117 GN++ LL + L +Q HG I +V + G+ + DGL T Sbjct: 18 FGNKSALLPDCLKPYQASQANKKQVHG--IQIVDVTEVGQACGE-------ADGLYTTQA 68 Query: 118 GVLLTSFYADCVPLYFYDPLKKVVGLAHAGWKGTVAEIAAAMVARMETGYGSRPQDILAG 177 G+LL+ ADC+P+ F K V + HAGW+G + I MV R+ P + +A Sbjct: 69 GILLSVLTADCLPIIFSQRHGKGVAVVHAGWRGLLEGIVLQMVERISR--DDNPTNWVAA 126 Query: 178 IGPSIGDCCYEVDDYVMD 195 IGP+ CCYEV++ +++ Sbjct: 127 IGPAARSCCYEVNEELVE 144 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,989,522 Number of extensions: 982191 Number of successful extensions: 2309 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2296 Number of HSP's successfully gapped: 6 Length of query: 285 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 180 Effective length of database: 26,268,361 Effective search space: 4728304980 Effective search space used: 4728304980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)