BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0025.p (1030 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBPI058 [J] COG0060 Isoleucyl-tRNA synthetase 645 0.0 SPBC8D2.06 [J] COG0060 Isoleucyl-tRNA synthetase 645 0.0 YBL076c [J] COG0060 Isoleucyl-tRNA synthetase 641 0.0 YPL040c [J] COG0060 Isoleucyl-tRNA synthetase 254 4e-67 SPCC18B5.08c [J] COG0060 Isoleucyl-tRNA synthetase 251 4e-66 >SPBPI058 [J] COG0060 Isoleucyl-tRNA synthetase Length = 346 Score = 645 bits (1664), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/1030 (0%), Positives = 0/1030 (0%), Gaps = 51/1030 (4%) Query: 16 RILKKWSDENTFRRSMENREGRPNYVFYEGPPTANGVPHIGHVLGRVIKDFIGRYQTMKG 75 + + + Y N + + G +T G Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 76 YRVVRKAGWDTHGLPVELGVQKKLGISGKQEIEEYGVEKFIKECKESVFGYEKQWREFTE 135 + + + + ++ + G + + + + +S+ + + + + Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 136 AIGYWTDLDHPYVTLDNTYIESVWNILATVHEKGLLYRGHRVSPYCPSCQTTLSSHEVAQ 195 + + + L + Y+ Y T Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 196 GYKTVKDLSATAKFK-LNGSGDYVLAWTTTPWTLPAHMALAMNPDMEYVRARQEDG--VY 252 DL + + + + L + + + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 253 VLAKN----LAEEVLKGEYTILSTHKGTDFIGQSYTPPFGYIQA---EKHNVIVGASFVT 305 GE T + D + P A + V T Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 306 DSSGTGIVHMAPAHGEDDYKSCRENGISFVN-----VVDTSGKYTDVVSDFAGRFVKDCD 360 + + + V Sbjct: 311 TMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP 370 Query: 361 LDIVKVLSEKGLLYSKEKYEHSYPFCWRCDTPLLYYATDSWFIQTTAIKDQLIANNNSVD 420 Sbjct: 371 430 Query: 421 WYPDHVREGRFGKFLEELVDWNISRNRYWGTPLNIWICQDTGKEFAPHSIAELRSMAIGE 480 Sbjct: 431 -- 488 Query: 481 VPEDIELHKPYVDNIKLRSPFSEG-GVMVRTSEVIDVWFDSGSMPFAQSHYPFENQDKFE 539 Sbjct: 489 -- 546 Query: 540 DQYPADMICEGIDQTRGWFYSLLAVSTLFKGKAPYKAVIAHGHIFDENGQKMSKSKGNVI 599 Sbjct: 547 606 Query: 600 DPWEIMNEYGTDAFR-WAILSDSAPWNNKRFSRGLVGETKSKIVDTLVNTHAFL----TL 654 Sbjct: 607 666 Query: 655 YAGIDGYDPA-DHPFKVSGHKLDRWILSRLNSLILLVDKGLAVNDFVNTSKAVENFVDEL 713 Sbjct: 667 726 Query: 714 SNWYIRRSRDRFWGSGLGEEKLDAYRXXXXXXXXXXXXMAPFTPMLSEDIFMNLGGG--- 770 Sbjct: 727 786 Query: 771 -------ESVHLADYPVADESLIDLELEQDMESARQIVELARNVRNETGIKNRQPLSELI 823 Sbjct: 787 846 Query: 824 VSIDRSFHLAD---YEEIIKDEINVKEIVVEHSDSGF-VDFTLKLNLKVAGKKYGKNVGF 879 Sbjct: 847 906 Query: 880 LQGFLKALDSEATRKAVQEGVIAIVSPEGEELQITAEELLVEKQAKPGFASASGYGLT-V 938 Sbjct: 907 --- 963 Query: 939 ALNTEITPELEQEGWVREIVRAVQDYRKRLDLPIEKRIALTLKVDGE---LKAAVTA--- 992 Sbjct: 964 1023 Query: 993 -FEHVLRENV 1001 Sbjct: 1024 1033 >SPBC8D2.06 [J] COG0060 Isoleucyl-tRNA synthetase Length = 346 Score = 645 bits (1664), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/1030 (0%), Positives = 0/1030 (0%), Gaps = 51/1030 (4%) Query: 16 RILKKWSDENTFRRSMENREGRPNYVFYEGPPTANGVPHIGHVLGRVIKDFIGRYQTMKG 75 + + + Y N + + G +T G Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 76 YRVVRKAGWDTHGLPVELGVQKKLGISGKQEIEEYGVEKFIKECKESVFGYEKQWREFTE 135 + + + + ++ + G + + + + +S+ + + + + Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 136 AIGYWTDLDHPYVTLDNTYIESVWNILATVHEKGLLYRGHRVSPYCPSCQTTLSSHEVAQ 195 + + + L + Y+ Y T Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 196 GYKTVKDLSATAKFK-LNGSGDYVLAWTTTPWTLPAHMALAMNPDMEYVRARQEDG--VY 252 DL + + + + L + + + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 253 VLAKN----LAEEVLKGEYTILSTHKGTDFIGQSYTPPFGYIQA---EKHNVIVGASFVT 305 GE T + D + P A + V T Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 306 DSSGTGIVHMAPAHGEDDYKSCRENGISFVN-----VVDTSGKYTDVVSDFAGRFVKDCD 360 + + + V Sbjct: 311 TMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP 370 Query: 361 LDIVKVLSEKGLLYSKEKYEHSYPFCWRCDTPLLYYATDSWFIQTTAIKDQLIANNNSVD 420 Sbjct: 371 430 Query: 421 WYPDHVREGRFGKFLEELVDWNISRNRYWGTPLNIWICQDTGKEFAPHSIAELRSMAIGE 480 Sbjct: 431 -- 488 Query: 481 VPEDIELHKPYVDNIKLRSPFSEG-GVMVRTSEVIDVWFDSGSMPFAQSHYPFENQDKFE 539 Sbjct: 489 -- 546 Query: 540 DQYPADMICEGIDQTRGWFYSLLAVSTLFKGKAPYKAVIAHGHIFDENGQKMSKSKGNVI 599 Sbjct: 547 606 Query: 600 DPWEIMNEYGTDAFR-WAILSDSAPWNNKRFSRGLVGETKSKIVDTLVNTHAFL----TL 654 Sbjct: 607 666 Query: 655 YAGIDGYDPA-DHPFKVSGHKLDRWILSRLNSLILLVDKGLAVNDFVNTSKAVENFVDEL 713 Sbjct: 667 726 Query: 714 SNWYIRRSRDRFWGSGLGEEKLDAYRXXXXXXXXXXXXMAPFTPMLSEDIFMNLGGG--- 770 Sbjct: 727 786 Query: 771 -------ESVHLADYPVADESLIDLELEQDMESARQIVELARNVRNETGIKNRQPLSELI 823 Sbjct: 787 846 Query: 824 VSIDRSFHLAD---YEEIIKDEINVKEIVVEHSDSGF-VDFTLKLNLKVAGKKYGKNVGF 879 Sbjct: 847 906 Query: 880 LQGFLKALDSEATRKAVQEGVIAIVSPEGEELQITAEELLVEKQAKPGFASASGYGLT-V 938 Sbjct: 907 --- 963 Query: 939 ALNTEITPELEQEGWVREIVRAVQDYRKRLDLPIEKRIALTLKVDGE---LKAAVTA--- 992 Sbjct: 964 1023 Query: 993 -FEHVLRENV 1001 Sbjct: 1024 1033 >YBL076c [J] COG0060 Isoleucyl-tRNA synthetase Length = 346 Score = 641 bits (1654), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/1023 (0%), Positives = 0/1023 (0%), Gaps = 46/1023 (4%) Query: 16 RILKKWSDENTFRRSMENREGRPNYVFYEGPPTANGVPHIGHVLGRVIKDFIGRYQTMKG 75 L + + + + + + + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAK 75 Query: 76 YRVVRKAGWDTHGLPVELGVQKKLGISGKQEIEEYGVEKFIKECKESVFGYEKQWREFTE 135 + K + K + + + + + + + Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQ 135 Query: 136 AIGYWTDLDHPYVTLDNTYIESVWNILATVHEKGLLYRGHRVSPYCPSCQTTLSSHEVAQ 195 T + + + A + + + H + Sbjct: 136 RPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADK 195 Query: 196 GYKTVKDLSATAKFKLNGS-GDYVLAWTTTPWTLPAHMALAMNPDMEYVRARQE--DGVY 252 LN S + + + + G Sbjct: 196 HDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGAR 255 Query: 253 VLAKNLAEEVLKG----EYTILSTHKGTDFIGQSYTPPFGYIQAEKHNV---IVGASFVT 305 + + + + Sbjct: 256 NTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQL 315 Query: 306 DSSGTGIVHMAPAHGEDDYKSCRENGI-----SFVNVVDTSGKYTDVVSDFAGRFVKDCD 360 ++ + + ++ + Sbjct: 316 KTNIESLHLELSEDVLAEIEAVHQVYT 375 Query: 361 LDIVKVLSEKGLLYSKEKYEHSYPFCWRCDTPLLYYATDSWFIQTTAIKDQLIANNNSVD 420 Sbjct: 376 435 Query: 421 WYPDHVREGRFGKFLEELVDWNISRNRYWGTPLNIWICQDTGKEFAPHSIAELRSMAIGE 480 Sbjct: 436 -- 493 Query: 481 VPEDIELHKPYVDNIKLRSPFSEG-GVMVRTSEVIDVWFDSGSMPFAQSHYPFENQDKFE 539 Sbjct: 494 -- 551 Query: 540 DQYPADMICEGIDQTRGWFYSLLAVSTLFKGKAPYKAVIAHGHIFDENGQKMSKSKGNVI 599 Sbjct: 552 611 Query: 600 DPWEIMNEYGTDAFR-WAILSDSAPWNNKRFSRGLVGETKSKIVDTLVNTHAFL----TL 654 Sbjct: 612 671 Query: 655 YAGIDGYDPADHPFKVSGHKLDRWILSRLNSLILLVDKGLAVNDFVNTSKAVENFVDELS 714 Sbjct: 672 731 Query: 715 NWYIRRSRDRFWGSGLGEEKLDAYRXXXXXXXXXXXXMAPFTPMLSEDIFMNL------- 767 Sbjct: 732 791 Query: 768 ------GGGESVHLADYPVADESLIDLELEQDMESARQIVELARNVRNETGIKNRQPLSE 821 Sbjct: 792 851 Query: 822 LIVSIDRSFHLADYEEI---IKDEINVKEIVVEHSDSGF-VDFTLKLNLKVAGKKYGKNV 877 Sbjct: 852 911 Query: 878 GFLQGFLKALDSEATRKAVQEGVIAIVSPEGEELQITAEELLVEKQAKPGFASASGYGLT 937 Sbjct: 912 971 Query: 938 VALNTEITPELEQEGWVREIVRAVQDYRKRLDLPIEKRIALTLKVDGELKAAVTAFEHVL 997 Sbjct: 972 ---- 1027 Query: 998 REN 1000 Sbjct: 1028 1030 >YPL040c [J] COG0060 Isoleucyl-tRNA synthetase Length = 346 Score = 254 bits (650), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 0/632 (0%), Positives = 0/632 (0%), Gaps = 69/632 (10%) Query: 40 YVFYEGPPTANGVPHIGHVLGRVIKDFIGRYQTMKGYRVVRKAGWDTHGLPVELGVQKKL 99 V G+ + + I++ + + P + Sbjct: 87 KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGY 146 Query: 100 GISGKQEIEEYGVEKF-IKECKESVFGYEKQWREFTEAIGYWTDLDHPYVTLDNTYIESV 158 + + E + + K + + + ++ + V Sbjct: 147 SWTDSAPAVSLLDTLDALAEYQRAG----KIRYIGVSNETAFGVMRYLHLADKHDLPRIV 202 Query: 159 WNILATVHEKGLLYRGHRVSPYCPSCQTTLSSHEVAQGYKTVKDLSATAKFKL------- 211 G + S T K L+ Sbjct: 203 TIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT-LTGKYLNGAKPAGARNTLFSR 261 Query: 212 --------NGSGDYVLAWTTTPWTLPAHMALAMNPDMEYVRARQEDGVYVLAKNLAEE-- 261 L + A G + + Sbjct: 262 FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIES 321 Query: 262 VLKGEYTILSTHKGTDFIGQSYTPPFGYIQAEKHNVIVGASFVTDSSGTGIVHMAPAHGE 321 Sbjct: 322 -- 379 Query: 322 DDYKSCRENGISFVNVVDTSGKYT---------DVVSD----FAGRFVKDCDLD--IVKV 366 Sbjct: 380 439 Query: 367 LSEKGLLYSKEKYEHSYPFCWRCDTPLLYYATDSWFIQTTAIKDQLIANNNSVDWYPDHV 426 Sbjct: 440 - 498 Query: 427 REGRFGKFLEELVDWNISRNRYWGTPLNIWICQD------TGKEFAPHSIAELRSMAIGE 480 Sbjct: 499 558 Query: 481 VPEDIELHKPYVDNIKLRSPFSEGGVM---VRTSEVIDVWFDSGSMPFAQSHYPFENQDK 537 Sbjct: 559 ------- 611 Query: 538 FED-QYPADMIC-EGIDQTRGWFYS--LLAVSTLFKGKAPYKAVIAHGHIFDENGQKMSK 593 Sbjct: 612 671 Query: 594 SKGNVIDPWEIMN--------EYGTDAFRWAI 617 Sbjct: 672 703 >SPCC18B5.08c [J] COG0060 Isoleucyl-tRNA synthetase Length = 346 Score = 251 bits (641), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 0/631 (0%), Positives = 0/631 (0%), Gaps = 75/631 (11%) Query: 32 ENREGRPNYVFYEGPPTANGVPHIGHVLGRVIKDFIGRYQTMKGYRVVRKAGWDTHGLPV 91 + + + + + + R V Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 92 ELGVQKKLGISGKQEIEEYGVEKFIKECKESVFGYEKQWREFTEAIGYWTDLDHPYVTLD 151 Q+ GK + I Y T Sbjct: 132 H-WPQRPTNCFGKLGYS---WTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS---NETAF 184 Query: 152 NTYIESVWNILATVHEKGLLYRGHRVSPYCPSCQTTLSSHEVAQGYKTVKDLSATAKFKL 211 + + + + S Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC-LGFGTLTG 243 Query: 212 NG--------SGDYVLAWTTTPWTLPAHMALAMNPDMEYVRARQEDGVYVLAKNLAEEV- 262 + + + + T A+A D+ + + V Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 263 -LKGEYTILSTHKGTDFIGQ-SYTPPFGYIQAEKHNVIVGASFVTDSSGTGIVHMAPAHG 320 T + I Sbjct: 304 STLLGATTMDQ--LKTNIESLHLELS--- 358 Query: 321 EDDYKSCRENGISFVNVVDTSGKYTDVVSDFAGR---FVKDCDLDIVKVLSEKGLLYSKE 377 Sbjct: 359 418 Query: 378 KYEHSYPFCWRCDTPLLYYATDSWFIQTTAIKDQLIANNNSVDWYPDHVREGRFGKFLEE 437 Sbjct: 419 - 477 Query: 438 LVDWNISRNRYWGTPLNIWICQDTGKEFAPHSIAELRSMAIGEVPEDIEL---------- 487 Sbjct: 478 ------- 530 Query: 488 ----HKPYVDNIKLRSPFSEGGVMVRTSEVIDVWFDSGSMPFAQSHYPFENQDKFEDQYP 543 Sbjct: 531 ------------- 577 Query: 544 ADMICEGIDQTRGWFYSLLAVSTLFKGK--APYKAVIAHGHIFDENGQKMSKSKGNVIDP 601 Sbjct: 578 - 636 Query: 602 WEIMN---------EYGTDAFR-WAILSDSA 622 Sbjct: 637 667 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 79,737,108 Number of extensions: 3468180 Number of successful extensions: 7526 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 7471 Number of HSP's successfully gapped: 21 Length of query: 1030 Length of database: 38,378,431 Length adjustment: 116 Effective length of query: 914 Effective length of database: 24,999,687 Effective search space: 22849713918 Effective search space used: 22849713918 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 121 (51.2 bits)