BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0014.p (1337 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1649 [Q] COG3321 Polyketide synthase modules and related prot... 376 e-103 all2635 [Q] COG3321 Polyketide synthase modules and related prot... 357 1e-97 ML2354 [Q] COG3321 Polyketide synthase modules and related proteins 351 4e-96 ML0135 [Q] COG3321 Polyketide synthase modules and related proteins 342 4e-93 all2646 [Q] COG3321 Polyketide synthase modules and related prot... 338 5e-92 >all1649 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1570 Score = 376 bits (965), Expect = e-103, Method: Compositional matrix adjust. Identities = 220/615 (35%), Positives = 313/615 (50%), Gaps = 44/615 (7%) Query: 732 IAVIAMACRFPGASSPEEFWHNLLQGKDSIGEVPQDRWNIDDYYSPTPEF-GKIYCRNGG 790 IA+I + CRFPGA SP+ FW L G D+I E+P +RWNID++Y PE GK+ R GG Sbjct: 4 IAIIGIGCRFPGADSPQAFWQLLSNGVDAITEIPAERWNIDEFYDRNPETPGKMNARYGG 63 Query: 791 FLDNPYGFDAALFGISGDEAAVMDPQQRIVMELVYELIERAGYSRQQMNGRDVGLFVGAG 850 FL FD FGIS EA +MDPQQR+++E+ +E +E AG R+Q+ G G+FVG Sbjct: 64 FLSQVDRFDPHFFGISPREALLMDPQQRLLLEVAWEALEDAGIVREQLTGSKTGVFVGIS 123 Query: 851 GNSYFEYHLNTLNRMNLQSFDSFSTLTSVQQERIMEEWKRKLGVTGTHPNLLVDNIINMI 910 N Y H N Q +D L N IN+ Sbjct: 124 TNDYSRIHPEF--DSNPQGYD------------------------------LTGNCINIA 151 Query: 911 PARTSQEFNFKGPSMAVDTACSSSLVTLHLAADSIRRGECESAIAGGIHLMLTPTSYQYF 970 R S FN +GPS+AVDTACSSSLV +HLA SI E AIA G++L+L+P Sbjct: 152 AGRLSYLFNLRGPSLAVDTACSSSLVAVHLACQSIWNQESSMAIASGVNLVLSPIGNIAL 211 Query: 971 SSAEALSPTGRSSVFAADADGFVPGEGAGLVMLKPLEQALRDGDPVLAVLKASGINNDGH 1030 S +ALSP GR F A+G+V EGAG ++LKP+ QAL D DP+ AV++ S IN+DG Sbjct: 212 SKLKALSPDGRCKTFDESANGYVRSEGAGCIILKPIAQALADHDPIYAVIRGSAINHDGR 271 Query: 1031 SIGVMAPNPDGQRELIESLYIRHNLSPADVQYVEAHGTGTKIGDPSEVRALDSAFKRWGL 1090 S G+ P Q LI + + P ++ YVE HGTGT +GDP E A+ + Sbjct: 272 SKGLTVPYGPAQESLIRQALQQAQVQPKEISYVELHGTGTPLGDPIEAMAIGAVLGEGRD 331 Query: 1091 LRQSVAIGSVKXXXXXXXXXXXXXXFIKVVMALQHKIMPPQINVSAPNPMLKFEKTPFYL 1150 +G+VK IK+ +AL+++ +PP ++ PNP + F++ P + Sbjct: 332 AAHPCLVGAVKSNIGHLEAAAGIASIIKMALALKYQQIPPSLHFHQPNPYIPFDQLPLRV 391 Query: 1151 LAAAQEWPVAEGKARRAAINSFGFGGTNSHMVVEEAPARASAGEAEQPVRAKHVIGLSAQ 1210 + WP ++ A +A ++SFGF GTN+H+++E + S +Q + H++ LSA Sbjct: 392 QTSLIPWPKSKYGA-KAGVSSFGFSGTNAHVILEGVSSFNSP--EQQTCKFPHLLPLSAH 448 Query: 1211 TEHALQQKMLELAAFLEQHGDYP--LADVCYTENAARTALPHRFHAVTDSVQDLIGKLQT 1268 T A+Q L + P + ++CY+ + RT +R V +S +DL LQ Sbjct: 449 TPAAVQTLALGYQDLINAQKLTPEFVQNLCYSASVRRTHQSYRSAVVVNSPEDLPSCLQA 508 Query: 1269 GXXXXXXXXXXXXX-----XLMFTGQGSQYAGMGRALYDGLPAFRKNVDACSAAFEPYLD 1323 +F+GQG Q+ MGR L P FR ++ C A + Y Sbjct: 509 LATADITTQTKQSKRKPKVAFVFSGQGPQWWAMGRELLAQEPVFRAVIEECDALIQKYAQ 568 Query: 1324 LK-LTDLIXXXXKSR 1337 LT+ +SR Sbjct: 569 WSLLTEFAVPESQSR 583 >all2635 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1565 Score = 357 bits (915), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 205/604 (33%), Positives = 315/604 (52%), Gaps = 37/604 (6%) Query: 732 IAVIAMACRFPGASSPEEFWHNLLQGKDSIGEVPQDRWNIDDYYSPTPEFGKIYCRNGGF 791 +A++ MA RFPGA + +EFW NL G +SI + + + + E + Y R Sbjct: 39 VAIVGMAGRFPGAKNVDEFWDNLKNGINSIKFLSNEDLLANGVKAEDLE-KQNYVRAYAS 97 Query: 792 LDNPYGFDAALFGISGDEAAVMDPQQRIVMELVYELIERAGYSRQQMNGRDVGLFVGAGG 851 L FDA FG S EA ++DPQ R+ +E +E +E AGY +Q +G ++ ++ GA Sbjct: 98 LAGIDEFDAGFFGYSPREAEILDPQHRLFLECAWEALENAGYDSEQYSG-NIAVYGGAAL 156 Query: 852 NSYFEYHLNTLNRMNLQSFDSFSTLTSVQQERIMEEWKRKLGVTGTHPNLLVDNIINMIP 911 NSY +NL S ++ + Q +++ N++ ++P Sbjct: 157 NSYL---------VNLASNSNYRENSDRTQ-------------------VVISNVMGLMP 188 Query: 912 ARTSQEFNFKGPSMAVDTACSSSLVTLHLAADSIRRGECESAIAGGIHLMLTPTSYQYFS 971 R S + N G S + T CS+SLV++H+A S+ EC+ A+AGG+ + + + + Sbjct: 189 TRVSYKLNLTGASCGIQTGCSTSLVSVHIACQSLLNKECDIALAGGVSIGIGEKAGYLYQ 248 Query: 972 SAEALSPTGRSSVFAADADGFVPGEGAGLVMLKPLEQALRDGDPVLAVLKASGINNDG-H 1030 LSP G F +A G V G G G+V+LK L A++DGD + A++KA+ INNDG Sbjct: 249 EDGVLSPDGYCRAFDVNAKGTVFGNGVGIVVLKRLRDAIQDGDHIYAIIKATAINNDGSQ 308 Query: 1031 SIGVMAPNPDGQRELIESLYIRHNLSPADVQYVEAHGTGTKIGDPSEVRALDSAFKRWGL 1090 +G+ AP+ GQ + I + + ++P +QY+E HGTGT +GDP E+ AL AF Sbjct: 309 KVGLTAPSVTGQAKAITTALTKAKINPETIQYIETHGTGTALGDPIEIAALTKAFSPHTH 368 Query: 1091 LRQSVAIGSVKXXXXXXXXXXXXXXFIKVVMALQHKIMPPQINVSAPNPMLKFEKTPFYL 1150 +Q A+GSVK IK +AL+HK +PP +N +A NP + F +PF++ Sbjct: 369 QKQFCAVGSVKTNIGHLDAAAGISGLIKTALALKHKQIPPSLNFTAANPQIDFANSPFFV 428 Query: 1151 LAAAQEWPVAEGKARRAAINSFGFGGTNSHMVVEEAPARASAGEAEQPVRAKHVIGLSAQ 1210 W + RRA ++SFG GGTN+H ++EEAP + ++ + H++ LSA+ Sbjct: 429 NTQLTPW-INHQHPRRAGVSSFGMGGTNAHAILEEAPTQFKIQNSKFKIN-YHLLCLSAK 486 Query: 1211 TEHALQQKMLELAAFLEQHGDYPLADVCYTENAARTALPHRFHAVTDS----VQDLIGKL 1266 +E AL+Q + LA L++H + LADV YT R A HR V + ++ L G Sbjct: 487 SEKALEQATINLATHLQKHPELNLADVAYTLQIGRRAFNHRRCLVCQTPAQAIEILTGVD 546 Query: 1267 QTGXXXXXXXXXXXXXXLMFTGQGSQYAGMGRALYDGLPAFRKNVDACSAAFEPYLDLKL 1326 + MF GQGSQY MG+ LY+ P FRK +D CS +P+L L Sbjct: 547 ASQLLSYSPENTEPSIVFMFPGQGSQYLNMGQELYNTEPVFRKEIDHCSELLQPHLGCDL 606 Query: 1327 TDLI 1330 ++ Sbjct: 607 PSIL 610 >ML2354 [Q] COG3321 Polyketide synthase modules and related proteins Length = 311 Score = 351 bits (901), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 0/606 (0%), Positives = 0/606 (0%), Gaps = 63/606 (10%) Query: 732 IAVIAMACRFPG-ASSPEEFWHNLLQGKDSIGEVPQDRWNIDDYYSPTPEF-GKIYCRNG 789 +++ ++ E + K + + + D + Sbjct: 38 GLFLSVILNTSKISNGIENNVRIVAYMKLDVHDQETKIQDPKDNKKTINNPDHLKIYDSI 97 Query: 790 GFLDNPYGFDAALFGISGDEAAVMDPQQRIVMELVYELIERAGYSRQQMNGRDVGLFVGA 849 + N + + E + + + A + Sbjct: 98 KKVPNVKTVEFSSKEAQLKELTKALGNNWNLFKGDANPLYDAYIVEADKPANVKSVTSKI 157 Query: 850 GGNSYFEYHL-NTLNRMNLQSFDSFSTLTSVQQERIMEEWKRKLGVTGTHPNLLVDNIIN 908 S T N Sbjct: 158 ---SKLSGIEKATYGGANTAKLFQ----------------------IAQGIRTWG----- 187 Query: 909 MIPARTSQEFNFKGPSMAVDTACSSSLVTLHLAADSIRRGECESAIAGGIHLMLTPTSYQ 968 + F + +T + L G I L Sbjct: 188 ---TAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFFLEGAWVGLL 244 Query: 969 YFSSAEALSPTGRSSVFAADADGFVPGEGAGLVMLKPLEQALRDGDPVLAVLKASGINND 1028 + F + + A + + + + ++ + G Sbjct: 245 GSIIPGLIVAWAYRLAFVSLTPSLQSQKLAMFAPKEFIPAIVGLMAVIGILIGSFGALLS 304 Query: 1029 GHSIGVMAPNPDGQRELIESLYIRHNLSPADVQYVEAHGTGTKIGDPSEVRALDSAFKRW 1088 Sbjct: 305 364 Query: 1089 GLLRQSVAIGSVKXXXXXXXXXXXXXXFIKVVMALQHKIMPPQINVSAPNPMLKFEKTPF 1148 Sbjct: 365 --- 421 Query: 1149 YLLAAAQEWPVAEGKARRAAINSFGFGGTNSHMVVEEAPARA---SAGEAEQPVRAKHVI 1205 Sbjct: 422 ---- 477 Query: 1206 GLSAQTEHALQQKMLELAAFLEQHG-DYPLADVCYTENAARTALPH------RFHAVTDS 1258 Sbjct: 478 -- 535 Query: 1259 VQDL----IGKLQTGXXXXXXXXXXXXXXLMFTGQGSQYAGMGRALYDGLPAFRKNVDAC 1314 Sbjct: 536 --- 592 Query: 1315 SAAFEP 1320 Sbjct: 593 - 597 >ML0135 [Q] COG3321 Polyketide synthase modules and related proteins Length = 225 Score = 342 bits (876), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 0/612 (0%), Positives = 0/612 (0%), Gaps = 51/612 (8%) Query: 732 IAVIAMACRFPGA-SSPEEFWHNLLQGKDSIGEVPQDR-WNIDDYYSPTPEF-GKIYCRN 788 I F + ++ G Sbjct: 44 ELGIYDLILLDLMLPEKNGFEVLKELREQNVDTPVLIMTAKESLDDKMHGFDIGADDYLT 103 Query: 789 GGFLDNPYGFDAALFGISGDEAAVMDPQQRIVMELVYELIERAGYSRQQMNGRDVGLFVG 848 F + + + + L + V Sbjct: 104 KPFYLDELKARIQALLKRTGKLEDSNGLTYGNIRLNLSNKSTLVDDQPVDLIGKEFDLVV 163 Query: 849 AGGNSYFEYHLNTLNRMNLQSFDSFSTLTSVQQERIMEEWKRKLGVTGTHPNLLVDNIIN 908 + N + + G + Sbjct: 164 YLMQNQ-----NVILPKEQIFD----------------------RIWGY--------DSD 188 Query: 909 MIPARTSQEFNFKGPSMAVDTACSSSLVTLHLAADSIRRGECESAIAGGIHLMLTPTSYQ 968 Sbjct: 189 248 Query: 969 YFSSAEALSPTGRSSVFAADADGFVPGEGAGLVMLKPLEQALRDGDPVLAVLKASGINND 1028 Sbjct: 249 308 Query: 1029 GHSIGVMAPNPDGQRELIESLYIRHNLSPADVQYVEAHGTGTKIGDPSEVRALDSAFKRW 1088 Sbjct: 309 368 Query: 1089 GLLRQSVAIGSVKXXXXXXXXXXXXXXFIKVVMALQHKIMPPQINVSAPNPMLKFEKTPF 1148 Sbjct: 369 428 Query: 1149 YLLAAAQEWPVAEGKARRAAINSFGFGGTNSHMVVEEA--PARASAGEAEQPVRAKHVIG 1206 Sbjct: 429 - 487 Query: 1207 LSAQTEHALQQKMLELAAFLEQHGDYPLADV-CYTENAARTALPHRFHAVTDSVQDLIGK 1265 Sbjct: 488 -- 545 Query: 1266 L------QTGXXXXXXXXX-XXXXXLMFTGQGSQYAGMGRALYDGLPAFRKNVDACSAAF 1318 Sbjct: 546 605 Query: 1319 EPYLDLKLTDLI 1330 Sbjct: 606 617 >all2646 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1488 Score = 338 bits (866), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 199/601 (33%), Positives = 310/601 (51%), Gaps = 36/601 (5%) Query: 732 IAVIAMACRFPGASSPEEFWHNLLQGKDSIGEVPQDRWNIDDYYSPTPEFGKIYCRNGGF 791 IA+I + +FPGA++ EE+W NL G +SI + ++ + PT Y + Sbjct: 9 IAIIGINGKFPGANNLEEYWQNLRNGVESITNLTDEQIQ-KNGVDPTQLKHPNYVKRQAI 67 Query: 792 LDNPYGFDAALFGISGDEAAVMDPQQRIVMELVYELIERAGYSRQQMNGRDVGLFVGAGG 851 +DN FDA FG + EA ++DPQ R+ +E + +E AGY+ + G +G++ GA Sbjct: 68 IDNIEYFDAEFFGFNPREAEILDPQHRLFLECAWTALEDAGYNPENYTGA-IGVYAGAAM 126 Query: 852 NSYFEYHLNTLNRMNLQSFDSFSTLTSVQQERIMEEWKRKLGVTGTHPNLLVDNIINMIP 911 N+Y +NL +T+ Q + + ++ L + N + + Sbjct: 127 NNYL---------LNL--------ITNPQIQNQISRYQ-----------LFLSNDKDFLT 158 Query: 912 ARTSQEFNFKGPSMAVDTACSSSLVTLHLAADSIRRGECESAIAGGIHLMLTPTSYQYFS 971 R S + N +GPS+ + TACS+SLV +H+A S+ GEC+ A+AGG+ L T Y Y Sbjct: 159 TRVSYKLNLRGPSLDIQTACSTSLVAVHVACQSLLSGECDIALAGGVSLA-KATGYLY-Q 216 Query: 972 SAEALSPTGRSSVFAADADGFVPGEGAGLVMLKPLEQALRDGDPVLAVLKASGINNDGH- 1030 SP G F ADA G + G G G+V+LK L++A+ D D + AV+K S INNDG Sbjct: 217 EGGIYSPDGHCRAFDADAQGTIAGSGLGIVVLKRLQEAIEDRDYIHAVIKGSAINNDGGL 276 Query: 1031 SIGVMAPNPDGQRELIESLYIRHNLSPADVQYVEAHGTGTKIGDPSEVRALDSAFKRWGL 1090 + AP D Q +I + + PAD+ Y+E HGTGT +GDP E+ AL AF+ Sbjct: 277 KVSYTAPRIDTQAAVIRAAQALAGVEPADISYIETHGTGTALGDPIEIAALTQAFRAGTE 336 Query: 1091 LRQSVAIGSVKXXXXXXXXXXXXXXFIKVVMALQHKIMPPQINVSAPNPMLKFEKTPFYL 1150 AI SVK IK V+AL+H+ +PP ++ PNP + F +TPFY+ Sbjct: 337 KTGFCAISSVKTNIGHLDAAAGIASLIKTVLALKHQQIPPNLHFQQPNPQIDFSQTPFYV 396 Query: 1151 LAAAQEWPVAEGKARRAAINSFGFGGTNSHMVVEEAPARASAGEAEQPVRAKHVIGLSAQ 1210 +W + RRA ++SFG GGTN+H+++EEAP + ++ + + ++ LSA+ Sbjct: 397 NTNLVDWET-DKTPRRAGVSSFGIGGTNAHVILEEAPIKFKIQNSKFKINYQ-MLCLSAK 454 Query: 1211 TEHALQQKMLELAAFLEQHGDYPLADVCYTENAARTALPHRFHAVTDSVQDLIGKLQTGX 1270 +E AL++ + LA +L+QH + LAD+ YT + R +R + + ++ L+ Sbjct: 455 SEKALEEATINLATYLQQHPQFNLADIAYTLHIGRQHFAYRRMVLCQTTEEAAQILEKET 514 Query: 1271 XXXXXXXXXX-XXXLMFTGQGSQYAGMGRALYDGLPAFRKNVDACSAAFEPYLDLKLTDL 1329 MF GQGS Y MG+ LY+ FR+ +D C + L L L + Sbjct: 515 KYTHTNSEKSPSIAFMFPGQGSHYLNMGKELYETEAIFREQIDHCCELLQHDLGLDLLSI 574 Query: 1330 I 1330 + Sbjct: 575 L 575 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 97,730,749 Number of extensions: 4226038 Number of successful extensions: 18274 Number of sequences better than 1.0e-05: 293 Number of HSP's gapped: 17655 Number of HSP's successfully gapped: 312 Length of query: 1337 Length of database: 38,378,431 Length adjustment: 118 Effective length of query: 1219 Effective length of database: 24,769,019 Effective search space: 30193434161 Effective search space used: 30193434161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)