BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0003.p (570 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0675 [G] COG1472 Beta-glucosidase-related glycosidases 195 2e-49 CAC0182 [G] COG1472 Beta-glucosidase-related glycosidases 140 7e-33 BB0620 [G] COG1472 Beta-glucosidase-related glycosidases 109 1e-23 mll9166 [G] COG1472 Beta-glucosidase-related glycosidases 99 2e-20 RSc0769 [G] COG1472 Beta-glucosidase-related glycosidases 98 4e-20 >BH0675 [G] COG1472 Beta-glucosidase-related glycosidases Length = 686 Score = 195 bits (496), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 159/558 (28%), Positives = 260/558 (46%), Gaps = 43/558 (7%) Query: 24 VRAAFGQMTVQEKIGQLF---FMVGYKQDE----PFLKHIAQEIGIGGLMC---RSMAKE 73 + QMT++EKIGQ+ F Q+ P ++ + +E +GG++ + E Sbjct: 131 IETLLDQMTLEEKIGQMMMPDFRTWNGQNVVEMLPEIEQMVKEYHLGGVILFRENVVETE 190 Query: 74 EVVATVATLQRSA-RIPLLIAANLEAGGQGITRDGTKIGSNMAIAATGSSDLAYQLGKIC 132 +++ V Q +A ++ LLI + E G ++GT + NMA+ AT S LA Q G + Sbjct: 191 QIIHLVNDYQDAAEKLGLLITIDQEGGIVTRLQNGTDLPGNMALGATRSEGLASQAGTVI 250 Query: 133 AVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGMAASV 192 E S+G N APV+D++ N NP+ R+F +P++V ++G AY KG Q G+AA+ Sbjct: 251 GRELQSLGINMNLAPVLDVNNNPDNPVIGVRSFSENPELVGQLGVAYTKGLQSTGVAATA 310 Query: 193 KHFPGDGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEYSKLL 252 KHFPG G + D HL D+ +R + EG +M H+ P Sbjct: 311 KHFPGHGDTDVDSHLGLPEVPHDRARLDEVELAPFRQAMAEGIDAIMTAHVTFPAIDDTK 370 Query: 253 NPSLRDEEI--LPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRAIE 310 S +D + LPA+L+ F G+IITDA M ++ + V R+I Sbjct: 371 VISEKDGSLISLPATLSEKVLTDLMRDDLGFEGVIITDAMNMNAISDHFGPTDAVIRSIN 430 Query: 311 NGCDMFLFTKNLEEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKAS---LKLHERENV-P 366 G D+ L L+ + E +E G +S R+++AV RIL LK + LK ++ Sbjct: 431 AGADIILMPVGLQTVFPAVVEAVENGEISEERVNEAVKRILTLKLNRGILKTDSSTSIDE 490 Query: 367 VLAESSAVLRQDSHLEIARAVADQSITLVKEEAGVLPLSPGRYPRVLLYDIESEANALGY 426 +AE+ V+ D H +I + +A+QSIT++K E LPLS + + +G Sbjct: 491 QIAEAERVIGSDDHRQIEQQIAEQSITVIKNEQDTLPLS---------VEEDDYVVVVGS 541 Query: 427 SKTNGLAAAFIPLLENEGFKVTRFEPAGVYEGLQSSFTEMKESYDLILYLCNLATKSNQT 486 + + LAAAF E+ + + QSS T E DL+ + S+ Sbjct: 542 TYSENLAAAF----EDASVQTEHI----LLSSNQSSLT--AEQQDLVEGASAIIIASH-- 589 Query: 487 TVRIEWLNPMGLNVPNYIH-----SIPTLFISTENPYHLLDVPRVKTYINTYGVNDYTLP 541 + I +P ++ Y+ P + ++ NPY ++ P V YI YG D + Sbjct: 590 SANIAGRSPSHPHMQLYVSLSESADQPVVAVAIRNPYDIMAYPSVPAYIAQYGFRDASFR 649 Query: 542 QLLAKLMGRSQFTGKSPI 559 + ++G +G+ P+ Sbjct: 650 ASVRTIVGDLTPSGQLPV 667 >CAC0182 [G] COG1472 Beta-glucosidase-related glycosidases Length = 520 Score = 140 bits (352), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 142/549 (25%), Positives = 235/549 (42%), Gaps = 69/549 (12%) Query: 36 KIGQLFFMVGY--KQDEPFLKHIAQEIGIGGLMCRSMAKEE---VVATVATLQR----SA 86 KIGQ+ M G+ K + L+ + + IG + S EE + +Q+ + Sbjct: 14 KIGQMI-MAGFTSKHYDSSLEKLITDDKIGNFILFSRNIEEKNQLTRLTEDIQKGLEINI 72 Query: 87 RIPLLIAANLEAGGQGITRDGTKI-GSNMAIAATGSSDLAYQLGKICAVEGGSVGANYAF 145 +IP +I+ + E G GT I NMA AA ++ GKI E +G N Sbjct: 73 KIPGIISVDQEGGMVTRINSGTTIFPGNMAFAAANHPHSTFRQGKIEGEELRGLGVNMNL 132 Query: 146 APVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGMAASVKHFPGDGVDERDQ 205 APV+D++ N NP+ R++ SP+ V E+G +KG +E + KHFPG G + D Sbjct: 133 APVMDVNCNPSNPVIGVRSYSDSPEKVAELGLELIKGLKESKVVPVAKHFPGHGDTDVDS 192 Query: 206 HLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEYSKLLNPSLRDEEILPAS 265 HL V + + + ++ IE G +M H+ PE + + LPA+ Sbjct: 193 HLSLPVVKHSIKRLRKVELLPFKTAIENGIDAIMSAHVLFPEI---------EPKRLPAT 243 Query: 266 LASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRAIENGCDMFLFTKNL--- 322 L+ F G+IITD M + ++ AI+ G D+ + + Sbjct: 244 LSYRVLTDLLRNKMGFKGIIITDCMEMKAIAEFYGSDKAAVMAIKAGADLICISHSAAVQ 303 Query: 323 EEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKASLKLHERENVPVLAESSAVLRQDSHLE 382 + +R +KE + +S R++++V RIL +K + S ++++ + Sbjct: 304 KACIRRIKEAVINKEISEERINESVKRILEIKEKYSI-----------SFNTVKRNYDIN 352 Query: 383 ---IARAVADQSITLVKEEAGVLPLSPGRYPRVLLYDIESEANALGYSKTN-GLAAAFIP 438 A ++D+SITL+K + ++PL GR + I ++A AL ++ N L +F Sbjct: 353 NSLFAEEISDKSITLLKNDKSLIPLK-GR-----IVSISTKAVALTGAEDNMSLNNSFCH 406 Query: 439 LLENEGFKVTRFEPAGVYEGLQSSFTEMKESYDLILYLC--------NLATKSNQTTVRI 490 L+ E F + F Q KE D IL C + N R Sbjct: 407 KLK-ENFGGSEF---------QIPINPHKEIIDKILEKCLDADSIVIGIYNAYNHEGQR- 455 Query: 491 EWLNPMGLNVPNYIHSIPTLFISTENPYHLLDVPRVKTYINTYGVNDYTLPQLLAKLMGR 550 + +N + PN + +S NPY L V YIN Y + ++ ++ L GR Sbjct: 456 KLVNEISKVNPNIV------LVSLRNPYDFLYFKEVSAYINAYEYTNLSVKSVIKVLSGR 509 Query: 551 SQFTGKSPI 559 + G SP+ Sbjct: 510 VEAQGISPV 518 >BB0620 [G] COG1472 Beta-glucosidase-related glycosidases Length = 555 Score = 109 bits (273), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 89/339 (26%), Positives = 149/339 (43%), Gaps = 49/339 (14%) Query: 14 FYLSEEDVKWVRAAFGQMTVQEKIGQLFFMVGYKQDE--PFLKHIAQEIGIGGLMCRSMA 71 F+ + E K + +M E +GQ+F M+ Y F+ + +GG+ Sbjct: 21 FWDTMEREKLINEMVSKMQDHELLGQMF-MISYPNQSITNFVLDFISKKNLGGIKIFGWN 79 Query: 72 KEEVVATVATLQRSAR--------IPLLIAANLEAG-GQGITRDGTKIGSNMAIAATGSS 122 +++ ++ ++ + IPL +A + E G Q I + ++ N+ IAA+ S Sbjct: 80 AKDLKNLTESIHKAQKTSQNNKFKIPLFVATDQEGGLAQHIKLNTSETIGNLGIAASLSP 139 Query: 123 DLAYQLGKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKG 182 +Y G A E +G N FAP+VDI + N RT+ +P++V + A+ KG Sbjct: 140 KDSYNTGYYIAQELRQLGINLNFAPIVDIYSHENNFAIGPRTYSDNPKIVSLLSLAFYKG 199 Query: 183 CQEQGMAASVKHFPGDGVDERDQH----------LVTSVNSLTPEAWDDTYGMVYRHLIE 232 ++ G+ ++ KHFPG G D H L S+N L P Y+ LI+ Sbjct: 200 QKQGGVISTAKHFPGHGNTTLDSHINIPIINSNLLEISLNELLP----------YKILIQ 249 Query: 233 EGAKTVMVGHIALPEYSKLLNPSLRDEEILPASLASXXXXXXXXXXXXFNGLIITDATTM 292 E +M GH+A P+ L + E +PAS + +N +IITD M Sbjct: 250 ENIPVIMTGHLAYPK--------LTNGENIPASSSIKIIKDILRKKLKYNNIIITDDLLM 301 Query: 293 AGMNIPLPRNE----LVPRAIENGCDMFLFTKNLEEDVR 327 +N NE + R + D+FL + L E+++ Sbjct: 302 NAVNY---NNESIYNTIERIVRTKSDIFLIS--LNENIQ 335 >mll9166 [G] COG1472 Beta-glucosidase-related glycosidases Length = 394 Score = 99.0 bits (245), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 85/309 (27%), Positives = 142/309 (45%), Gaps = 29/309 (9%) Query: 33 VQEKIGQLFFM--VGYKQDEPFLKHIAQEIGIGGL-----MCRSMAKEEVVATVAT-LQR 84 +++ IGQ+ + VG D+ K + ++ G + + R+++ E V + LQ+ Sbjct: 46 LRQMIGQMVLVGFVGDSPDDDGYKRVVKQAEAGEITGVIYLGRNISSLEAVRQLNKGLQQ 105 Query: 85 SARIPLLIAANLEAGGQGITRDGTKIG-----SNMAIAATGSSDLAYQLGKICAVEGGSV 139 A PLL+A + E G I R ++G S +A T S D A + + A + S+ Sbjct: 106 YATAPLLVAIDQEGGR--IQRLTGEVGFREVPSEATVAKTMSPDEASVVYQDLASDLSSL 163 Query: 140 GANYAFAPVVDIDYNFRNPITNT--RTFGSSPQVVMEMGAAYVKGCQEQGMAASVKHFPG 197 G N APVVD++ N NPI R+F + PQ V A+++ + +G+ ++KHFPG Sbjct: 164 GFNLNLAPVVDLNVNPANPIIGKLGRSFSADPQKVEAYARAFIEAHRAKGVLTALKHFPG 223 Query: 198 DGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEG-AKTVMVGHIALPEYSKLLNPSL 256 G +D H + +T + W D + Y+ LI G VM H+ +N L Sbjct: 224 HGSSTKDSH--KGIADVT-KTWSDKELLPYKGLIASGDVDMVMAAHV--------INAKL 272 Query: 257 RDEEILPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRAIENGCDMF 316 +PASL+ F G++I+D M + + + V RA+ G D+ Sbjct: 273 AFSPDIPASLSRATLTTLLRDNMHFKGVVISDDMQMQAITDNVSFEDSVLRAVMAGNDIL 332 Query: 317 LFTKNLEED 325 +F + D Sbjct: 333 IFANDKHPD 341 >RSc0769 [G] COG1472 Beta-glucosidase-related glycosidases Length = 734 Score = 97.8 bits (242), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 116/464 (25%), Positives = 191/464 (41%), Gaps = 56/464 (12%) Query: 29 GQMTVQEKIGQLFFMVGYKQDEP-----------FLKHIA----QEIGIGGLMCRS---M 70 GQM+ ++KIGQ M EP L A ++ IGG++ + + Sbjct: 90 GQMSTRDKIGQKIMMAFRYWCEPPADPNCTDRMTVLPDAAASALRDHRIGGVVLFANNLV 149 Query: 71 AKEEVVATVATLQRSARIPLLIAANLEAGG-----QGITRDGTKIGSNMAIAATGSSDLA 125 + +++ A + +A + +LI + E G +GI T NMA+ A Sbjct: 150 SVDQISRLTAQIAAAAPVGMLIGVDEEGGNVFRLPRGIA---TSFPGNMALGAAYLGAAD 206 Query: 126 ----YQLGKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVK 181 + G++ A E +VG N FAPVVD++ N NP+ N R +G P +V +G + Sbjct: 207 AALAHDEGRVLASELAAVGMNVNFAPVVDVNSNPLNPVINVRAYGDDPAMVGLLGRHMAR 266 Query: 182 GCQEQGMAASVKHFPGDGVDERDQHL-VTSVNSLTPEAWDDTYGMVYRHLIE--EGAKTV 238 G G+ ++ KHFPG G D H + V+ +A+ T YR I+ E + + Sbjct: 267 GMAHAGVISAFKHFPGHGDAATDSHYGLPRVDKSRADAY-ATDLAPYRASIDLGEAPEMI 325 Query: 239 MVGHIALPEYSKLLNPSLRDEEIL-PASLASXXXXXXXXXXXXFNGLIITDATTMAGMNI 297 M HI P + + E IL PA+++ + G+ ++DA M + Sbjct: 326 MTAHIQYPSLDETRVATRTGEGILVPATMSRKIQHDLLRGEFGYRGVTVSDALDMDAIAN 385 Query: 298 PLPRNELVPRAIENGCDMFLF---------TKNLEEDVRFMKEGLEAGWLSPGRLDDAVL 348 ++ V D+ L L + + L+AG LS LD +V Sbjct: 386 HFDPDQAVINLFRADVDIALMPVEFRTAGQADRLRAVIDRVVAALDAGTLSRAELDASVE 445 Query: 349 RILGLKASLKLHERENVPVLAESSA--VLRQDSHLEIARAVADQSITLVKEEAGVLPLSP 406 RI +K + + P ++SA V+ H + +A SITL++ LPL+ Sbjct: 446 RITLMKLRHGIAPNASRP---QTSAVTVIGSPGHRAAEQRIAQASITLLRNRGATLPLNA 502 Query: 407 GRYPRVLLYDIESEANA-------LGYSKTNGLAAAFIPLLENE 443 + P ++ +A LGY+ G A P E + Sbjct: 503 PQRPIFIMTPWGEQAQGMRQRFAELGYASVTGAKLAETPWAEQQ 546 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,465,178 Number of extensions: 1791141 Number of successful extensions: 12929 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 12888 Number of HSP's successfully gapped: 38 Length of query: 570 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 458 Effective length of database: 25,461,023 Effective search space: 11661148534 Effective search space used: 11661148534 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)