BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0033.p (289 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 108 1e-23 MA0185 [GEPR] COG0477 Permeases of the major facilitator superfa... 105 1e-22 Z2875 [GEPR] COG0477 Permeases of the major facilitator superfamily 103 3e-22 lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 95 1e-19 AF1144 [GEPR] COG0477 Permeases of the major facilitator superfa... 89 8e-18 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 108 bits (269), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 73/262 (27%), Positives = 129/262 (49%), Gaps = 10/262 (3%) Query: 8 SLPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGI 67 + +L+ RV+QA+G ++ + + IFP +RG A+G+IG + GP++ G+ Sbjct: 102 TFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGL 161 Query: 68 IVSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTL-FAGM 126 I+ L W +IFW+++P +IA++ G K + + K KID + ++ + TL F G+ Sbjct: 162 ILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKID-----ILSIILSTLGFGGV 216 Query: 127 LL----GQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCA 182 + + G+G + ++ + FK P+F+L ++ Sbjct: 217 VFAFSSAGENGWGSATVLVSIIVGGIALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILV 276 Query: 183 FLVFVANFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLT 242 F+ F+ I+ P Y QN L L+ F AG +L+ + +++P +G L D G L Sbjct: 277 FISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALV 336 Query: 243 FAGLIVMVIAQFGLARLHDGSA 264 G IV VIA F L R+ G++ Sbjct: 337 IPGFIVAVIALFFLTRIEVGTS 358 >MA0185 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 614 Score = 105 bits (261), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 79/268 (29%), Positives = 127/268 (47%), Gaps = 22/268 (8%) Query: 12 LIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGIIVST 71 LI R+IQA+G + TMAN I+TD+FPA ERGKALG+ + G G LGGI+ +T Sbjct: 127 LIIFRIIQAVGGAFTMANGAAIITDVFPAGERGKALGINMVALMSGQFIGLLLGGIL-AT 185 Query: 72 LGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRV-KSKIDLPGSLLFAVFIVTLFAGMLLG- 129 W Y+F VN+P L+ +L ++ + + R K+ ID+ G+L F + +L G+ G Sbjct: 186 YNWRYVFLVNIPFALLGTILSYRKMKEVSFRAPKTSIDIVGNLFFIGGLTSLLVGVTYGL 245 Query: 130 ---------QQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSIL 180 +G+ + +I+ E FK F+ + L Sbjct: 246 MPYEHNGITDAMGWSNPLVIATVGIGILLLIAFPFVESRVKNPMFRLEFFKIRAFTYANL 305 Query: 181 CAFLVFVAN---------FCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGA 231 +F +A I P + + +PF+AG ++ + +I+ P+SG Sbjct: 306 ASFTAAIARGGIMFMLILLLQGIWLPLHGYRFED-TPFWAGIYMLPMTLGFIIMGPISGI 364 Query: 232 LSDKIGSEFLTFAGLIVMVIAQFGLARL 259 LSDK G ++ AG+ ++ + GLA L Sbjct: 365 LSDKYGPRWIATAGMTLVTLVFVGLAML 392 >Z2875 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 494 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 73/256 (28%), Positives = 127/256 (49%), Gaps = 8/256 (3%) Query: 8 SLPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGI 67 SL +L +RVIQ G + M+ + ++ I+P G+ +G+ V++ S AGP++ Sbjct: 140 SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIAAA 199 Query: 68 IVSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRV-KSKIDLPGSLLFAV---FIVTLF 123 I+S W+++F +NVP+G+IA++L + LP + R K + DLP +++ A+ ++T Sbjct: 200 ILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLLITAL 259 Query: 124 AGMLLGQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAF 183 +G GQ + + I + + + PLFSLSI + Sbjct: 260 SGFAQGQSL----TLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICTSV 315 Query: 184 LVFVANFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTF 243 F A + PFY Q +L S G LL +P++ +++APL+G L +++ + L Sbjct: 316 CSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAGLLGA 375 Query: 244 AGLIVMVIAQFGLARL 259 GL +M F L L Sbjct: 376 LGLFIMAAGLFSLVLL 391 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 69/262 (26%), Positives = 125/262 (47%), Gaps = 8/262 (3%) Query: 11 LLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGIIVS 70 +LIA R++QAIGA + M +V ++FP RG+A+GLIG ++ GP+L G IV Sbjct: 102 MLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGWIVQ 161 Query: 71 TLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTLFAGMLLG- 129 W +F++ +P ++ IV+ +L R K+D+ G ++ V F +LLG Sbjct: 162 QYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKLDILGVIMSTVG----FGSLLLGF 217 Query: 130 QQVGYGD---SRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVF 186 G D ++ + FK P F+L+ +F V Sbjct: 218 SNAGDHDWLTWKVAGFIILGLVVLGIFIRYQSSNKAPLLNFKVFKYPTFALTTAISFFVV 277 Query: 187 VANFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGL 246 + F ++ P + Q + SP +G +L+ + I++P++G + D+ G+++L+ GL Sbjct: 278 MGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSPVTGVMFDRFGAKYLSLVGL 337 Query: 247 IVMVIAQFGLARLHDGSAVALV 268 I+M + F L + + + + Sbjct: 338 IIMAGSTFMFTNLDESTTLTFI 359 >AF1144 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 451 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 69/243 (28%), Positives = 104/243 (42%), Gaps = 9/243 (3%) Query: 11 LLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGIIVS 70 +LIA RV+Q +G +M A S I+T +FP ERGKA+G+ V G GP GG + Sbjct: 96 MLIAFRVLQGVGGAMIFATSVAILTSVFPPQERGKAIGINTGAVYTGLSLGPIGGGFLTQ 155 Query: 71 TLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVK-SKIDLPGSLLFAVFIVTLFAGMLLG 129 LGW +F N +G A+++ L + + K DL GS ++A ML Sbjct: 156 NLGWRSLFIANGALGFFALIVASVYLRGEWADARGEKFDLAGS--------AIYAAMLFF 207 Query: 130 QQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVFVAN 189 G+ + + N FSLS L A L + A Sbjct: 208 LIYGFSEFSVAFIVLGAILALIFVVYENRQEHPVFAVRLLLSNLTFSLSSLAALLNYAAT 267 Query: 190 FCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGLIVM 249 F + Y Q + AG +L+ P+ M I AP++G +SD+I + G+ V Sbjct: 268 FAVGFLMSLYLQYVKGFDAQTAGLILVSQPVVMAIFAPIAGWISDRIEPRVVASTGMAVT 327 Query: 250 VIA 252 ++ Sbjct: 328 TLS 330 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.144 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,827,265 Number of extensions: 708209 Number of successful extensions: 2854 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 2842 Number of HSP's successfully gapped: 56 Length of query: 289 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 184 Effective length of database: 26,268,361 Effective search space: 4833378424 Effective search space used: 4833378424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)