BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0030.p (386 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr2770 [G] COG2814 Arabinose efflux permease 293 5e-79 SMb20071 [G] COG2814 Arabinose efflux permease 275 7e-74 STM0257 [G] COG2814 Arabinose efflux permease 271 2e-72 AGl1145 [G] COG2814 Arabinose efflux permease 270 2e-72 mlr2471 [G] COG2814 Arabinose efflux permease 254 2e-67 >mlr2770 [G] COG2814 Arabinose efflux permease Length = 1091 Score = 293 bits (749), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 21/340 (6%), Positives = 48/340 (14%), Gaps = 1/340 (0%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 G + G I + + + T + Sbjct: 10 QGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTCYRLVWNHMT 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 ++ L L A + G + + Sbjct: 70 GAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTLANHDNQIVF 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQT-AN 179 + P + A + + A Sbjct: 130 GTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAG 189 Query: 180 LSEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGV 239 + ++G L A G + G Sbjct: 190 GGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPD 249 Query: 240 TLGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPG 299 G + + ++ N + + T+ Sbjct: 250 AENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQ 309 Query: 300 IQLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAIT 339 + + T G Sbjct: 310 YVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQV 349 >SMb20071 [G] COG2814 Arabinose efflux permease Length = 397 Score = 275 bits (704), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 155/353 (43%), Positives = 241/353 (68%), Gaps = 6/353 (1%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 + A+AIG TEF+I+GLLP VA DL +++P AG +++ YALGV GAPVLT T R+ +K Sbjct: 10 IAAYAIGTTEFVIVGLLPTVATDLAITLPLAGLIVSVYALGVTFGAPVLTALTGRIERKP 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL+ LM +FI GN + ++P Y +L++AR+L+AFAHG F VG+ IAA LV +RA A+ Sbjct: 70 LLLGLMALFIGGNTAAALSPNYEVLLVARVLSAFAHGVFFSVGSTIAADLVPENRRASAI 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIP-VIVQEQTAN 179 +++ GLTVA + GVP GT+IGQ GWRA+F ++ LGVV+ GI +P + + A+ Sbjct: 130 AMMFMGLTVAIVTGVPIGTYIGQVFGWRATFWGVSGLGVVAFAGIATLLPGTLAKAAPAS 189 Query: 180 LSEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGV 239 L +QVR L + ++L++ ALG F FT++ P+L+E+TGF+E +V+ ILVL+G + Sbjct: 190 LLDQVRVLGSGRLLIVFAMTALGYGGTFVAFTFLAPILQEVTGFSERSVSLILVLYGVAI 249 Query: 240 TLGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPG 299 +GN+ GG++A+ + +LI F + A+++ +FS+T +PVLT++ + G +F +PG Sbjct: 250 AIGNIAGGRIANTNPVKALIGLFLLQALVLVIFSFTAVSPVLTLVTLAALGFLSFANVPG 309 Query: 300 IQLRIMNLA--HK--APLLATTSSHSALNLGNATGAYLGGAAI-TQWGLSSIP 347 +QL ++ LA H+ A +A+ + +A NLG A GA+LGG + + +GLS+ P Sbjct: 310 LQLYVVQLAMEHRPGAVDVASALNIAAFNLGIALGAWLGGMVVDSPFGLSATP 362 >STM0257 [G] COG2814 Arabinose efflux permease Length = 391 Score = 271 bits (692), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 151/353 (42%), Positives = 229/353 (64%), Gaps = 6/353 (1%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 + AFAIG TEF+I+GL+P +A+ L +S+P AG L++ YALGVAIGAPVLT T RLP+K+ Sbjct: 10 ISAFAIGTTEFVIVGLVPTIAQQLAISLPSAGMLVSIYALGVAIGAPVLTALTGRLPRKQ 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL+ LM +F GN ++ AP Y LI+AR+LT AHG F +G+ IA LV EK A A+ Sbjct: 70 LLVALMVLFTAGNLLAWQAPGYMTLIVARLLTGLAHGVFFSIGSTIATSLVPKEKAASAI 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPV-IVQEQTAN 179 +++ GLTVA + GVP GTFIGQ GWR +F A+++LGV++L IP I A+ Sbjct: 130 AIMFGGLTVALVTGVPLGTFIGQHFGWRETFLAVSLLGVIALMSSQLLIPANIPGRAAAS 189 Query: 180 LSEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGV 239 + +QV+ L +P++LLI ALG +F+ FT++ PM++++ GF+ V+WIL+ +G V Sbjct: 190 IRDQVKVLTHPRLLLIYAVTALGYGGVFTAFTFLAPMMQDLAGFSPAAVSWILLGYGVSV 249 Query: 240 TLGNLIGGKLADWK-LMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMP 298 +GN+ GGKLAD +P+L FA L +++ +F T + + + G+ AFG +P Sbjct: 250 AIGNIWGGKLADKHGAVPALKFIFAALFVLLMVFQVTASTQYAALATILVMGVFAFGNVP 309 Query: 299 GIQLRIMNLAHK----APLLATTSSHSALNLGNATGAYLGGAAITQWGLSSIP 347 G+Q+ ++ A + A +A+ + +A N+G A G+ +GG + +GL+ P Sbjct: 310 GLQVYVVQKAEQFTPNAVDVASGLNIAAFNIGIALGSVIGGQTVEHYGLAQTP 362 >AGl1145 [G] COG2814 Arabinose efflux permease Length = 134 Score = 270 bits (691), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 0/353 (0%), Positives = 0/353 (0%), Gaps = 6/353 (1%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 A A+ + + ++ + G V+ + Sbjct: 48 AKAPAVLQKLAQAVAAEGFSGIQINKALSSIDAHQETSGSGRIQTLRVVARQKGAAVRID 107 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 Sbjct: 108 167 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQ-TAN 179 Sbjct: 168 227 Query: 180 LSEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGV 239 Sbjct: 228 287 Query: 240 TLGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPG 299 Sbjct: 288 347 Query: 300 IQLRIMNLAHK----APLLATTSSHSALNLGNATGAYLGGAAI-TQWGLSSIP 347 Sbjct: 348 400 >mlr2471 [G] COG2814 Arabinose efflux permease Length = 1091 Score = 254 bits (649), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 23/350 (6%), Positives = 58/350 (16%), Gaps = 3/350 (0%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 G + G I + + + T + Sbjct: 10 QGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTCYRLVWNHMT 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 ++ L L A + G + + Sbjct: 70 GAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTLANHDNQIVF 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIV--QEQTA 178 + P + A + + Sbjct: 130 GTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAG 189 Query: 179 NLSEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAG 238 +N + G + ++ + + A V G Sbjct: 190 GGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPD 249 Query: 239 VTLGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMP 298 G+ D I+ + + ++ ++ G Sbjct: 250 AENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQ 309 Query: 299 GIQLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQ-WGLSSIP 347 + + GN T G + +++ Sbjct: 310 YVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQVDAGGTATNVT 359 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,087,110 Number of extensions: 1059304 Number of successful extensions: 4544 Number of sequences better than 1.0e-05: 108 Number of HSP's gapped: 4466 Number of HSP's successfully gapped: 108 Length of query: 386 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 278 Effective length of database: 25,922,359 Effective search space: 7206415802 Effective search space used: 7206415802 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)