BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0024.p (319 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1911 [G] COG4209 ABC-type polysaccharide transport system, per... 216 4e-56 BH2112 [G] COG4209 ABC-type polysaccharide transport system, per... 192 8e-49 BH0484 [G] COG4209 ABC-type polysaccharide transport system, per... 186 3e-47 BS_lplB [G] COG4209 ABC-type polysaccharide transport system, pe... 175 7e-44 BH1065 [G] COG4209 ABC-type polysaccharide transport system, per... 141 1e-33 >BH1911 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 319 Score = 216 bits (549), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 120/312 (38%), Positives = 180/312 (57%), Gaps = 11/312 (3%) Query: 15 VRPSRRK-----LQNKYKLFLLALPFLAVTFVFYYLPVSGWIYAFYDFIPGIPLSETPYV 69 +R RRK L+ + L+L++LPF+A VF Y P+ GW AF +F PG+ E +V Sbjct: 12 IRVQRRKKILTTLKQQKYLYLMSLPFVAWVLVFNYFPLWGWTMAFQNFRPGLSFFEQEWV 71 Query: 70 GLKWFRTLVANPTQTGEVLRVLKNTVAMSSLGLATS-VFPVIFAILLTEIRAGWYRKFVQ 128 GL+ F L Q VL+NT+AMS +GL P++FA+LL E+R +++ VQ Sbjct: 72 GLQHFIELF----QDNRFYLVLRNTLAMSIMGLIVGFTMPILFAVLLNEVRHMVFKRTVQ 127 Query: 129 TLTTIPNFISWILVYALAFSLFSVDNGVVNHILVQLGVVSEEFNFLASSSHVWLTMIAWY 188 T++ +P+F+SW++V + + S+D G+VN +L+ G V + F+A W+ + Sbjct: 128 TVSYLPHFVSWVVVAGIVTKMLSIDGGIVNQLLMAFGFVDQPVQFMAKGEWFWVIVTLSD 187 Query: 189 WWKSXXXXXXXXXXXXXXXDQELYEAAEVDGASRFRKIWHITVPGIIPTFFVLLLLSIGN 248 WK D +LYEAA+VDGASR+R+IWHIT+PGI PT V+L+LSIG+ Sbjct: 188 LWKEMGWNSIIFLAAMAGIDPQLYEAAKVDGASRWRQIWHITLPGIRPTILVILILSIGH 247 Query: 249 FVNNGMEQYFAFQNAMNKDAIEVLDLYVYNIAFA-NNFPFATAVSMLKSVVSVILLFAAN 307 + G E+ F N + D EVLDLY N + + TA+ + SVV +ILLF AN Sbjct: 248 LMAIGFEKQFLLGNPLVVDYSEVLDLYALNYGLGLGRYSYGTAIGIFNSVVCIILLFLAN 307 Query: 308 TLSKVVRDESII 319 + K ++S++ Sbjct: 308 GIFKRFTNQSVM 319 >BH2112 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 337 Score = 192 bits (487), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 108/295 (36%), Positives = 169/295 (57%), Gaps = 8/295 (2%) Query: 20 RKLQNKYKLFLLALPFLAVTFVFYYLPVSGWIYAFYDFIPGIPLSETPYVGLKWFRTLVA 79 +K + L L++LPFL +F Y+P+ GW AF DF P P+ + + G K F L Sbjct: 40 KKCYEQKALLLMSLPFLIWLIIFRYVPLYGWSIAFQDFRPARPIFDQDWAGFKHFTFLFT 99 Query: 80 NPTQTGEVLRVLKNTVAMSSLGLATSVFPVI-FAILLTEIRAGWYRKFVQTLTTIPNFIS 138 +P VL+NT+AMS + L I AILL E+R +++ VQT++ +P+F+S Sbjct: 100 DP----RFFEVLRNTLAMSLINLVLGFVTAIGLAILLNELRNLTFKRVVQTISYMPHFLS 155 Query: 139 WILVYALAFSLFSVDNGVVNHILVQLGVVSEEFNFLASSSHVWLTMIAWYWWKSXXXXXX 198 W++ L + S++NG++N +L++LG++SE +L + W + A WK+ Sbjct: 156 WVVAAGLVSAALSIENGIINIVLMKLGMISEPIMWLGVGEYFWGIIGASDVWKNVGWNTI 215 Query: 199 XXXXXXXXXDQELYEAAEVDGASRFRKIWHITVPGIIPTFFVLLLLSIGNFVNNGMEQYF 258 D E YEAAE+DGA+R ++IW+IT+PG+ P +LL++++G + +G E + Sbjct: 216 IYLAAITMIDTEQYEAAEIDGATRLQRIWYITLPGMRPVIVILLIMNLGYILESGFEAQY 275 Query: 259 AFQNAMNKDAIEVLDLYV--YNIAFANNFPFATAVSMLKSVVSVILLFAANTLSK 311 N MN D E LD++V Y I NF ATA + K+VVS I LF+AN ++K Sbjct: 276 LLMNPMNMDFAENLDIFVLRYGINMG-NFSLATAAGIFKTVVSFIFLFSANHIAK 329 >BH0484 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 325 Score = 186 bits (473), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 114/314 (36%), Positives = 172/314 (54%), Gaps = 15/314 (4%) Query: 10 RGVEPVRPSRRKLQNKYKLFLLALPFLAVTFVFYYLPVSGWIYAFYDFIPGIPLSETPYV 69 + V + +R +NKY L+L+ LP L +F Y+P+ G I AF D+ P + ++ + +V Sbjct: 21 KKVRRQQKLKRIFRNKY-LYLMILPGLVYFLIFKYVPMWGLIIAFQDYQPFLGVTGSEWV 79 Query: 70 GLKWFRTLVANPTQTGEVLRVLKNTVAMSSLGLATSVF----PVIFAILLTEIRAGWYRK 125 G K F L PT ++L+NT+ L L +F P+I A+LL EIR +++ Sbjct: 80 GFKHFERLFTEPT----FFQLLRNTLV---LFLYNLIFFFPVPIILAVLLNEIRLMVFKR 132 Query: 126 FVQTLTTIPNFISWILVYALAFSLFSVDNGVVNHILVQLGVVSEEFNFLASSSHVWLTMI 185 FVQT+ IP+F+SW++V +L + LF+ D GV+N I+ + + NFL S I Sbjct: 133 FVQTMIYIPHFMSWVIVVSLTYVLFTTDGGVINEII--YSITGKNVNFLMSEDWFRPLYI 190 Query: 186 AWYWWKSXXXXXXXXXXXXXXXDQELYEAAEVDGASRFRKIWHITVPGIIPTFFVLLLLS 245 W+ D LYEAA++DGA+RFR+IWHIT+P I VLL+L Sbjct: 191 LQVIWREAGWATIIYFAAIAAIDPSLYEAAKMDGANRFRQIWHITLPAIRSVIVVLLILK 250 Query: 246 IGNFVNNGMEQYFAFQNAMNKDAIEVLDLYVYNIAFAN-NFPFATAVSMLKSVVSVILLF 304 IG+ + G E F NAMN++ E+ D YVY F ++TAV KS+V +I++ Sbjct: 251 IGDILELGFEHVFLMLNAMNREVAEIFDTYVYTAGLKEGQFSYSTAVGFFKSIVGLIMVM 310 Query: 305 AANTLSKVVRDESI 318 AN L+K +E + Sbjct: 311 LANWLAKKFGEEGV 324 >BS_lplB [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 318 Score = 175 bits (444), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 100/280 (35%), Positives = 163/280 (58%), Gaps = 8/280 (2%) Query: 41 VFYYLPVSGWIYAFYDFIPGIPLSETPYVGLKWFRTLVANPTQTGEVLRVLKNTVAMSSL 100 +F YLP+ G + AF D+ P + ++ +VG +F+ NP + R+L+NT+ ++SL Sbjct: 44 IFKYLPMWGVLIAFKDYSPYLGFWKSEWVGFDYFKDFFMNP----DFFRLLRNTLMLASL 99 Query: 101 GLATS-VFPVIFAILLTEIRAGWYRKFVQTLTTIPNFISWILVYALAFSLFSVDNGVVNH 159 L + P+I A+LL E+R Y++ +QT +P+F+SW +V ++ F F+VD GV+N Sbjct: 100 DLLFAFPAPLILALLLNEVRKAVYKRCIQTFIYVPHFVSWTIVVSITFVFFTVDTGVINK 159 Query: 160 ILVQLGVVSEEFNFLASSSHVWLTMIAWYWWKSXXXXXXXXXXXXXXXDQELYEAAEVDG 219 +++ L E+ +FL+ + ++ WK DQE YEAA +DG Sbjct: 160 LIMSL--TGEQISFLSDADWFRPMIVMQSIWKETGWGTILFLAALATVDQEQYEAAIMDG 217 Query: 220 ASRFRKIWHITVPGIIPTFFVLLLLSIGNFVNNGMEQYFAFQNAMNKDAIEVLDLYVYNI 279 A RFR++WHIT+P I T VLL+L IG+F+N G EQ + N++N+ ++ D YVY + Sbjct: 218 AGRFRRMWHITLPAIRSTIIVLLILRIGSFLNLGFEQVYLMTNSLNRSVADIFDTYVYMM 277 Query: 280 AFANN-FPFATAVSMLKSVVSVILLFAANTLSKVVRDESI 318 + ++TAV + KSVV +IL+F AN ++K E + Sbjct: 278 GITQGAYSYSTAVGLFKSVVGIILIFGANYIAKKFDQEGL 317 >BH1065 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 317 Score = 141 bits (356), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 87/300 (29%), Positives = 159/300 (53%), Gaps = 8/300 (2%) Query: 13 EPVRPSRRKLQNKYKLFLLALPFLAVTFVFYYLPVSGWIYAFYDFIPGIPLSETPYVGLK 72 ++ + + ++L +L +P L + +F Y P+ G + AF D+ P + + + +VGL+ Sbjct: 15 RKLKKQKWNFRRTWQLHVLLIPALVLALIFQYGPMFGIVMAFQDYKPWLGFTGSEWVGLQ 74 Query: 73 WFRTLVANPTQTGEVLRVLKNTVAMSSLGLATS-VFPVIFAILLTEIRAGWYRKFVQTLT 131 F T+ + +V+ NT+ +S L + + P+I A+LL E+ ++ VQT+ Sbjct: 75 HFITMF----EYDYAKQVIWNTLVISVLKMIFGLIVPIIVALLLNEMYQMVVKRTVQTII 130 Query: 132 TIPNFISWILVYALAFSLFSVDNGVVNHILVQLGVVSEEFNFLASSSHVWLTMIAWYWWK 191 +P F+SW+++ + + S D G+VN L G+ + FL S+ T++ WK Sbjct: 131 YLPYFLSWVILGGILIDVLSPDGGIVNQALGLFGI--DPIFFLGSNDWFRTTVVISDLWK 188 Query: 192 SXXXXXXXXXXXXXXXDQELYEAAEVDGASRFRKIWHITVPGIIPTFFVLLLLSIGNFVN 251 + LYEAA VDGA+R+++ HIT+P ++P V+ LS+GN +N Sbjct: 189 ETGFNTIVFLAAITAVNPNLYEAAVVDGANRWKQTLHITLPAMLPIIIVVATLSLGNILN 248 Query: 252 NGMEQYFAFQNAMNKDAIEVLDLYVYNIAFAN-NFPFATAVSMLKSVVSVILLFAANTLS 310 G +Q F N + +++D YVY ++ N +F + TAV + KS++S++L+ L+ Sbjct: 249 AGFDQIFNLYNPLVYQTGDIIDTYVYRVSLVNGDFSYGTAVGLFKSLISLVLIIIGYRLA 308 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.140 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,886,518 Number of extensions: 778397 Number of successful extensions: 2914 Number of sequences better than 1.0e-05: 91 Number of HSP's gapped: 2876 Number of HSP's successfully gapped: 91 Length of query: 319 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 213 Effective length of database: 26,153,027 Effective search space: 5570594751 Effective search space used: 5570594751 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)