BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0023.p (311 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2000 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 226 3e-59 SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 207 2e-53 Ta0750 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 206 6e-53 BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 204 1e-52 SSO2773 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 204 2e-52 >BH2000 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 226 bits (577), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 12/318 (3%), Positives = 41/318 (12%), Gaps = 23/318 (7%) Query: 1 MKLMNFWESGNLRLGVKKEEGILDVXXX---------XXXXXXXXXXXVPATLREVLDGG 51 M+ S + V Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 52 EAALDSLRKFADSLPYHD---EGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEET 108 L + + + P + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNN-DKGI-RPDQALDRKNIREALHDSL 118 Query: 109 GMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAV 168 +Y L+ + T + L + + + V Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 169 FGYFAANDLSARDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISC 228 A + L + + ++ + Sbjct: 179 SNETAFGVMRYLHLADKHDLPR-------IVTIQNPYSLLNRSFEVGLAEVSQYEGVELL 231 Query: 229 SVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQP 288 + + + + Sbjct: 232 AYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAY--VDIARRHGLDPA 289 Query: 289 GDEVTVEIEKLGRLTNRM 306 + + + + Sbjct: 290 QMALAFVRRQPFVASTLL 307 >SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 207 bits (526), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 15/227 (6%), Positives = 35/227 (15%), Gaps = 10/227 (4%) Query: 84 FAPCTADPGKIICIGLNYRRHAEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVD 143 T + G + + +L + L S +++ Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 144 YEAELGIMIGRTAKNVSEEEALDAVFGYFAANDLSARDLQT-RTSQWLGGEXXXXAGKSC 202 + + + + L + A L Q Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQR-------AGKIRY 175 Query: 203 DRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSP 262 + + V N S + + S Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC 235 Query: 263 GDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIEKLGRLTNRMVAE 309 + E+ + V Sbjct: 236 LGFGTLTGKYLNGAKPAGAR--NTLFSRFTRYSGEQTQKAVAAYVDI 280 >Ta0750 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 206 bits (523), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 17/215 (7%), Positives = 33/215 (15%), Gaps = 12/215 (5%) Query: 92 GKIICIGLNYRRHAEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIM 151 + + + S + Sbjct: 112 EALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAG 171 Query: 152 IGRTAKNVSEEEALDAVFGYFAANDLSARDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPY 211 R + + FG L+ + R S + Sbjct: 172 KIR-----YIGVSNETAFGVMRYLHLADKHDLPRIVTIQ-------NPYSLLNRSFEVGL 219 Query: 212 VVTEDEVGDPGLLRISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTP 271 G L + N + + S + + Sbjct: 220 AEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVD 279 Query: 272 EGVMMGYPPEKQVYLQPGDEVTVEIEKLGRLTNRM 306 G P + + V LG T Sbjct: 280 IARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQ 314 >BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 204 bits (519), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 9/228 (3%), Positives = 27/228 (11%), Gaps = 10/228 (4%) Query: 83 EFAPCTADPGKIICIGLNYRRHAEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKV 142 A + + T Sbjct: 82 LIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCF 141 Query: 143 DYEAELGIMIGRTAKNVSEEEALDAVFGYFAANDLSARDLQT-RTSQWLGGEXXXXAGKS 201 + +AL + + ++ Sbjct: 142 GKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRY-------LHLAD 194 Query: 202 CDRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLS 261 + + + ++ E + S + F + + Sbjct: 195 KHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGA 254 Query: 262 PGDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIEKLGRLTNRMVAE 309 + T L Sbjct: 255 RNTLFSRFTRYSGEQTQKAVA--AYVDIARRHGLDPAQMALAFVRRQP 300 >SSO2773 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Length = 346 Score = 204 bits (518), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 14/266 (5%), Positives = 50/266 (18%), Gaps = 9/266 (3%) Query: 45 REVLDGGEAALDSLRKFADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRH 104 + ++ E L+ + + P + G+ + Sbjct: 48 DVAEMYPVPPRPETQGLTETY--VGNWLAKHGSREKLIIASKVS-GPSRNNDKGIRPDQA 104 Query: 105 AEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEA 164 + + + L ++ L + + P + + + + Sbjct: 105 LDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDAL 164 Query: 165 LDAVFGYFAANDLSARDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLL 224 + + + ++L ++ + Sbjct: 165 AEYQRAGKIRYIGVSNETAFGVMRYLH--LADKHDLPRIVTIQNPYSLLNRSFEVGLAEV 222 Query: 225 RISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQV 284 V + + TL +G + V Sbjct: 223 SQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVA----AYV 278 Query: 285 YLQPGDEVTVEIEKLGRLTNRMVAEK 310 + + L + + Sbjct: 279 DIARRHGLDPAQMALAFVRRQPFVAS 304 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,034,934 Number of extensions: 971734 Number of successful extensions: 2927 Number of sequences better than 1.0e-05: 76 Number of HSP's gapped: 2775 Number of HSP's successfully gapped: 77 Length of query: 311 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 205 Effective length of database: 26,153,027 Effective search space: 5361370535 Effective search space used: 5361370535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)