BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0019.p (511 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfa... 380 e-105 lin0980 [GEPR] COG0477 Permeases of the major facilitator superf... 248 1e-65 DR0258 [GEPR] COG0477 Permeases of the major facilitator superfa... 222 1e-57 PA0246 [GEPR] COG0477 Permeases of the major facilitator superfa... 202 1e-51 CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator supe... 163 5e-40 >BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 426 Score = 380 bits (975), Expect = e-105, Method: Compositional matrix adjust. Identities = 195/412 (47%), Positives = 264/412 (64%), Gaps = 19/412 (4%) Query: 23 MASMDSTIVATAMGNIVGELGGMDKFVWVTSAYLVAEMTGMPIFGKLSDMYGRKKFFIFG 82 M++MD+TIVATAMGNIV +LG DKF WVT++Y+VA M GMPI+GKLSDMYGRK+F Sbjct: 24 MSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFSCLD 83 Query: 83 IIVFMAGSALCGTADTITQLAAYRALQGIGGGALVPIAFAIMFDAVPLETRGKLGGAFGA 142 + + GSALCG A T+ QL +RA+QGIGGGAL+PIAF I+FD P E RGK+ G FGA Sbjct: 84 LFFSLIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGA 143 Query: 143 VFGLSSIFGPLLGAYITDHIAWQWVFYINLPLGVIALVMVVSFYRESVEHSKHPIDWLGA 202 VFGLSS+ GPLLGA ITD I+W WVFYIN+P+G ++L ++ +Y+ES+EH K IDW GA Sbjct: 144 VFGLSSVLGPLLGAIITDSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGA 203 Query: 203 GTLLGAVISLMFALELGGKEYAWDSXXXXXXXXXXXXXXXXXXXXETKAKEPII------ 256 TL+ +++ LMFALELGGK Y W+S E KA++P Sbjct: 204 ITLVVSIVCLMFALELGGKTYDWNSIQIIGLFIVFAVFFIAFFIVEKKAEDPARYSARSS 263 Query: 257 SFALFRQRLYASSILCALFSGAAFIAASVYIPIFIQGVLGGSATNSGLVLLPMMVASVVT 316 A RQR IL L+ G FI +V+IP F+Q V SA ++G +L PMM+ SV+ Sbjct: 264 RMACLRQR----QILAFLYEG-IFIILAVFIPTFVQPVYASSAASAGFILTPMMIGSVIR 318 Query: 317 ASTGGFLMVKTSYRSLMVPXXXXXXXXXXXXXXXEPDASRLLVTFYMILIGLGVGASFSV 376 + GG K S+R+LM+ PD +R+ +T +M++ G GVG +FS+ Sbjct: 319 SMIGGIFQTKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTVFMMISGFGVGFNFSL 378 Query: 377 LSTAAIHGLTPQQRGSASATLNFIRSLGMTIGITAFGIIQSHYFSSKLTQVF 428 LS A+++ L P+ RG+A++T +F+RS GMT+G H+F + Q + Sbjct: 379 LSAASMNDLEPRFRGTANSTNSFLRSFGMTLG--------CHHFRNGCKQTY 422 >lin0980 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 491 Score = 248 bits (634), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 143/445 (32%), Positives = 233/445 (52%), Gaps = 8/445 (1%) Query: 23 MASMDSTIVATAMGNIVGELGGMDKFVWVTSAYLVAEMTGMPIFGKLSDMYGRKKFFIFG 82 MA+++ TIV+TAM IV +L G++ W+ S YL+ +PI+GKLSD+YGRK F+ Sbjct: 19 MAAIEGTIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGRKNIFVIA 78 Query: 83 IIVFMAGSALCGTADTITQLAAYRALQGIGGGALVPIAFAIMFDAVPLETRGKLGGAFGA 142 I+F+ GS+LCG A + QL +RA+QGIG G ++P I+ D P E R K+ G G+ Sbjct: 79 TIIFIIGSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAKVLGFMGS 138 Query: 143 VFGLSSIFGPLLGAYITDHIAWQWVFYINLPLGVIALVMVVSFYRESVEHSKHPIDWLGA 202 +G++ +FGPL+G ++ D ++W W+F+IN+P+G++ +++++ + RE VE K PID+LGA Sbjct: 139 AWGIAGVFGPLVGGFLVDQLSWHWIFFINVPIGLLTILLILLYLREEVERVKLPIDYLGA 198 Query: 203 GTLLGAVISLMFALELGGKEYAWDSXXXXXXXXXXXXXXXXXXXXETKAKEPIISFALFR 262 A++ L+FAL+ G+ W E +AK+PI+ F LF+ Sbjct: 199 SLFTVALLGLLFALQRAGESLNWTEPLVVILFAVSILLFVAFYFVEKRAKDPIMPFVLFK 258 Query: 263 QRLYASSILCALFSGAAFIAASVYIPIFIQGVLGGSATNSGLVLLPMMVASVVTASTGGF 322 + L A I +VYIP++ QG+LG AT +G +L P+ V ++ + GG Sbjct: 259 NPVVLIGNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWIIGSFIGGK 318 Query: 323 LMVKTSYRSLMVPXXXXXXXXXXXXXXXEPDASRLLVTFYMILIGLGVGASFSVLSTAAI 382 L++ R + + + +G G G F+ + Sbjct: 319 LLMTLGNRHTVGIGVLITAASGLILALFPASTNDIFFYLNSAFMGFGFGIIFTTTTVTVQ 378 Query: 383 HGLTPQQRGSASATLNFIRSLGMTIGITAFGIIQSHYFSSKLTQVFXXXXXXXXXXXMNF 442 + Q G A+A+ R++G TIG+ FG I F+S LT F +N Sbjct: 379 DAVPRFQTGIATASNTLFRTVGQTIGVAVFGTI----FNSVLTTQF----RAAAGSEVNR 430 Query: 443 KDPHALLSPETRAQIPPGILSKITD 467 + + L+SP+T + P ++ + D Sbjct: 431 SNLNQLISPQTSGNVNPDLVEPLRD 455 >DR0258 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 544 Score = 222 bits (565), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 141/409 (34%), Positives = 215/409 (52%), Gaps = 8/409 (1%) Query: 23 MASMDSTIVATAMGNIVGELGGMDKFVWVTSAYLVAEMTGMPIFGKLSDMYGRKKFFIFG 82 ++S++ T+V +AM ++ +LGG + W +AY + +PI G LSD YGR+ + G Sbjct: 33 LSSLNMTVVGSAMPRVISDLGGFHLYAWAFTAYSLTTTVTIPIVGTLSDRYGRRPLILLG 92 Query: 83 IIVFMAGSALCGTADTITQLAAYRALQGIGGGALVPIAFAIMFDA-VPLETRGKLGGAFG 141 I+VF GS G ++ QL A RA+QGIGGG L+ ++F + D P+E RG+ G G Sbjct: 93 IVVFALGSMGLGFVQSMEQLIALRAIQGIGGGTLMAMSFTAIADLFTPIE-RGRYQGYTG 151 Query: 142 AVFGLSSIFGPLLGAYITDHIAWQWVFYINLPLGVIALVMVVSFYR---ESVEHSKHPID 198 AV+G+SS+ GPL+G ++TDH W+ VF++NLP ++AL + F+R + K D Sbjct: 152 AVWGISSVVGPLVGGFLTDHFGWRSVFFLNLPFALLALFFIWRFFRLPAPTPGSVKGGFD 211 Query: 199 WLGAGTLLGAVISLMFALELGGKEYAWDSXXXXXXXXXXXXXXXXXXXXETKAKEPIISF 258 LGA L GAV +L A+ GG YAWDS + + I+ Sbjct: 212 ALGATLLAGAVTTLTLAVSWGGGTYAWDSPRILGLLGATLALGAWYAAHSARQERAILDL 271 Query: 259 ALFRQRLYASSILCALFSGAAFIAASVYIPIFIQGVLGGSATNSGLVLLPMMVASVVTAS 318 L R R + L A AA +Y+P+++QGV G SA+ SGL L P+M ++T++ Sbjct: 272 GLLRDRGILLASLAGFLVSAGMYAAILYLPLYMQGVRGSSASGSGLALAPLMGGMILTST 331 Query: 319 TGGFLMVKT-SYRSLMVPXXXXXXXXXXXXXXXEPDASRLLVTFYMILIGLGVGASFSVL 377 G L+ +T Y+ L+V L MIL+GLG+G S L Sbjct: 332 LSGQLVSRTGRYKRLIVGGALVASLALVLSSTLSLSTPLWLAVTMMILLGLGLGPVNSQL 391 Query: 378 STAAIHGLTPQQR-GSASATLNFIRSLGMTIGITAFGIIQSHYFSSKLT 425 T A+ P+Q+ GSA+ F R +G T+ ++ FG + + + S L+ Sbjct: 392 -TLAVQNAAPRQKLGSATGGNQFFRQIGGTLAVSLFGALVNAHLSHDLS 439 >PA0246 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 501 Score = 202 bits (513), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 123/407 (30%), Positives = 199/407 (48%), Gaps = 1/407 (0%) Query: 4 AQKSPKXXXXXXXXXXXXXMASMDSTIVATAMGNIVGELGGMDKFVWVTSAYLVAEMTGM 63 AQ P ++++D TIVA ++ I + +D WV SAY+VA M Sbjct: 5 AQNPPAVRTVLAGLMLSIFLSALDQTIVAVSLPAISADYADLDLLAWVISAYMVAMTVSM 64 Query: 64 PIFGKLSDMYGRKKFFIFGIIVFMAGSALCGTADTITQLAAYRALQGIGGGALVPIAFAI 123 PI+GKL D+YGR++ +F I+VF A S LCG A ++ QL R LQGIG G L+ ++ AI Sbjct: 65 PIYGKLGDLYGRRRLMLFAIVVFTAASLLCGLAQSMGQLVLARVLQGIGAGGLMAVSQAI 124 Query: 124 MFDAVPLETRGKLGGAFGAVFGLSSIFGPLLGAYITDHIAWQWVFYINLPLGVIAL-VMV 182 + D VP RG+ G F +++ ++S+ GP+LG +T++++W WVF+INLP+G++AL + Sbjct: 125 IGDIVPPRERGRYQGYFSSMYAIASVAGPVLGGLLTEYLSWHWVFWINLPIGLLALAISR 184 Query: 183 VSFYRESVEHSKHPIDWLGAGTLLGAVISLMFALELGGKEYAWDSXXXXXXXXXXXXXXX 242 + V + ID+LG ++ + +L+ A+ G+ AW Sbjct: 185 RTLVGLPVPRRQPVIDYLGIVLMVVGLTALLLAITQIGQGAAWSDPALLGLFALAALALL 244 Query: 243 XXXXXETKAKEPIISFALFRQRLYASSILCALFSGAAFIAASVYIPIFIQGVLGGSATNS 302 E + +EP++ LF R S L A F+ I+ +V +P+ Q V G A ++ Sbjct: 245 LFVAQERRFREPLVPMQLFAVRAATLSWLVAFFASFQAISLTVLVPLRYQSVTGAGADSA 304 Query: 303 GLVLLPMMVASVVTASTGGFLMVKTSYRSLMVPXXXXXXXXXXXXXXXEPDASRLLVTFY 362 L LLP+++ + A G L ++ P L Sbjct: 305 ALHLLPLVMGLPLGAYCCGRLTASLGRYKPLILLGAVLLPCGIAGLALVPVGDHWLSGLC 364 Query: 363 MILIGLGVGASFSVLSTAAIHGLTPQQRGSASATLNFIRSLGMTIGI 409 M+L G+ +G F AA + + + G A++ RSLG +G+ Sbjct: 365 MLLTGIAIGTQFPTSLVAAQNAVEQRHIGVATSNATLFRSLGGAVGV 411 >CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 458 Score = 163 bits (413), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 111/386 (28%), Positives = 186/386 (48%), Gaps = 4/386 (1%) Query: 26 MDSTIVATAMGNIVGELG-GMDKFVWVTSAYLVAEMTGMPIFGKLSDMYGRKKFFIFGII 84 +DSTIV A+ NI ++ W+++ Y +A M K +D +GRKK I GI+ Sbjct: 22 IDSTIVNIALPNITDYFKVSLNDTSWISTIYALALAVFMITAAKFADQFGRKKLMIIGIL 81 Query: 85 VFMAGSALCGTADTITQLAAYRALQGIGGGALVPIAFAIMFDAVPLETRGKLGGAFGAVF 144 +F SALCG ++++ L R +QGIGG + PIA + + E + GA GAV Sbjct: 82 IFGVSSALCGISNSLLFLIIMRFVQGIGGAIITPIALPMGIEIFGKERMNIVVGASGAVV 141 Query: 145 GLSSIFGPLLGAYITDHIAWQWVFYINLPLGVIALVMVVSFYRESVEHS-KHPIDWLGAG 203 GL++ GP LG + +I WQ VF++N+P + AL+++V ES +++ IDW G Sbjct: 142 GLAAASGPPLGGVLIKYINWQSVFFVNVPFAIAALILIVICVNESYDNTVSKDIDWFGVM 201 Query: 204 TLLGAVISLMFALELGGKEYAWDSXXXXXXXXXXXXXXXXXXXXETKAKEPIISFALFRQ 263 TL ++ L+FAL L GK+Y W S E K P+I LF++ Sbjct: 202 TLTISLFCLIFAL-LKGKDYGWTSTLIISLFLIFVVSLILFIIIELKVSSPMIEINLFKE 260 Query: 264 RLYASSILCALFSGAAFIAASVYIPIFIQGVLGGSATNSGLVLLPMMVASVVTASTGGFL 323 + +S +C + +G + + + ++Q VL + +++ + + + + G L Sbjct: 261 PTFTASSICYMITGFGLMCPLLILNYYLQNVLQYDTLEAAFIMMSVSLTGMFSTPIGSIL 320 Query: 324 MVKTSYRSLMVPXXXXXXXXXXXXXXXEPDASRLLVTFYMILIGLGVGASFSVLSTAAIH 383 K S R + S+ + F +I+ G+G+G S LS+ ++ Sbjct: 321 GKKVSPRIINFIGMLSLGIGIFRLSYLSYGMSKGTMIFDLIICGIGLGFSVQALSS-SVK 379 Query: 384 GLTPQQRGSASATLNFIRSLGMTIGI 409 + ++ G S +N R +G IGI Sbjct: 380 FIPKEKSGIGSGIVNAARQIGSCIGI 405 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,335,440 Number of extensions: 1025739 Number of successful extensions: 3960 Number of sequences better than 1.0e-05: 120 Number of HSP's gapped: 3884 Number of HSP's successfully gapped: 122 Length of query: 511 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 400 Effective length of database: 25,576,357 Effective search space: 10230542800 Effective search space used: 10230542800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)