BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0009.p (301 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3181 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel tran... 378 e-105 CAC0178 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel tran... 291 9e-79 CAC2374 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel tran... 280 2e-75 BS_appC [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel tran... 222 5e-58 BH0030 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel trans... 204 2e-52 >CAC3181 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel transport systems, permease components Length = 302 Score = 378 bits (970), Expect = e-105, Method: Compositional matrix adjust. Identities = 187/296 (63%), Positives = 235/296 (79%) Query: 5 REPQVQRQKSSLFRQSLKRLMKNRLXXXXXXXXXXMFALCFIGPFFSPYADNKINMAMMN 64 + ++ SL +Q+LKR++KN+L + F+GPFFSPY+ N I+++++ Sbjct: 5 KNKNIKTSNKSLSKQTLKRILKNKLAIMSIIFLISILIFNFLGPFFSPYSYNDIDVSLIK 64 Query: 65 KAPSLQHLLGTDALGRDILTRVMQAGRISLTVGLASMVLSVFIGALLGAVAGYYRGIADQ 124 + PS+ HLLGTD GRD+LTR+M AGRISLTVGLASM+LS+ +G++LG ++GYY GI D Sbjct: 65 QPPSISHLLGTDDYGRDVLTRLMYAGRISLTVGLASMILSLLLGSILGCLSGYYGGILDI 124 Query: 125 IIMRIADLLMTIPGLPLLFIFGALLSEWKVPTDYRMYIVMLMLSIVNWPGLARMVRGQML 184 I+MR++D+LM+IPGLPLL I ALLSE KVP+DYR+YIVMLMLS V WPGLARMVRGQ+L Sbjct: 125 ILMRLSDILMSIPGLPLLIIIAALLSELKVPSDYRLYIVMLMLSFVGWPGLARMVRGQVL 184 Query: 185 SLREREFMQAAVVLGLRDSRKLFHHLLPNVVPLLIVMATLNIGGAILSESVLSFFGLGVM 244 SLRE+ FM+AA +LGL D RK+FHHLLPN PLLIV+ATL+ ILSES LSF GLGV+ Sbjct: 185 SLREQNFMKAADILGLSDKRKIFHHLLPNTFPLLIVVATLSTADGILSESSLSFLGLGVI 244 Query: 245 PPTPTWGNMIDAANNMIDFQDHPWLWIPPGLSIFATVIAINIFGDGLRDVLDPKQK 300 PPTP+WGNMI+AANN+IDFQ WLWIPPGL+IF TVI+IN+ GD LRD LDPK K Sbjct: 245 PPTPSWGNMINAANNLIDFQKRSWLWIPPGLAIFLTVISINLLGDALRDALDPKMK 300 >CAC0178 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel transport systems, permease components Length = 301 Score = 291 bits (745), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 141/284 (49%), Positives = 202/284 (71%) Query: 18 RQSLKRLMKNRLXXXXXXXXXXMFALCFIGPFFSPYADNKINMAMMNKAPSLQHLLGTDA 77 + + RL K++L M +GP S Y+ + ++ + PSL HL+GTD Sbjct: 18 KMAWHRLKKDKLALAGLFIVLFMIIFAVLGPVVSCYSTDTMDYSNPTIGPSLHHLMGTDE 77 Query: 78 LGRDILTRVMQAGRISLTVGLASMVLSVFIGALLGAVAGYYRGIADQIIMRIADLLMTIP 137 LGRDILTR+M AGRISL VG+A++V+ + +G+ GA+AG+Y GI D IIMRIAD+ + +P Sbjct: 78 LGRDILTRLMYAGRISLAVGVAAVVVEIIVGSAFGAIAGFYGGIIDGIIMRIADIFLCVP 137 Query: 138 GLPLLFIFGALLSEWKVPTDYRMYIVMLMLSIVNWPGLARMVRGQMLSLREREFMQAAVV 197 P+L AL S+ K+ + +++ +M ++ I++WPGL R+VRGQ+L+LRE+EFM AA Sbjct: 138 FFPILITIAALFSDLKIKSQFKVPFLMFIIGILSWPGLCRIVRGQILTLREQEFMLAANA 197 Query: 198 LGLRDSRKLFHHLLPNVVPLLIVMATLNIGGAILSESVLSFFGLGVMPPTPTWGNMIDAA 257 LG++D +K+F HLLPN +P +IV ATLNIG AIL+ES LSF GLGV+PPTP+WGNM+ + Sbjct: 198 LGIKDRKKIFKHLLPNTIPSIIVSATLNIGSAILTESSLSFLGLGVVPPTPSWGNMVQSV 257 Query: 258 NNMIDFQDHPWLWIPPGLSIFATVIAINIFGDGLRDVLDPKQKR 301 +++ + +PWLW+P G+ I TV+AIN+ GDGLRD LDPK K+ Sbjct: 258 SDLYKLEHYPWLWMPAGICILLTVMAINLLGDGLRDALDPKLKK 301 >CAC2374 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel transport systems, permease components Length = 289 Score = 280 bits (717), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 123/252 (48%), Positives = 184/252 (73%) Query: 47 GPFFSPYADNKINMAMMNKAPSLQHLLGTDALGRDILTRVMQAGRISLTVGLASMVLSVF 106 GPF S Y ++ IN + + APS++H+LGTD +GRD+LTR+M GRIS+ +G+ S++L Sbjct: 38 GPFLSKYGEDYINYSNIKSAPSVEHILGTDKVGRDVLTRLMYGGRISIMIGIFSVLLQAI 97 Query: 107 IGALLGAVAGYYRGIADQIIMRIADLLMTIPGLPLLFIFGALLSEWKVPTDYRMYIVMLM 166 + +LG V+GYY G+ D +IMR D++M+IP LP+L + GA+ S+ K+ R+++++ + Sbjct: 98 MAVILGIVSGYYGGMLDTLIMRFTDVVMSIPVLPILIVLGAIFSDMKISDKERIFLIVAI 157 Query: 167 LSIVNWPGLARMVRGQMLSLREREFMQAAVVLGLRDSRKLFHHLLPNVVPLLIVMATLNI 226 LSI+ WP LAR+VRG++L L+ +EFM AA +LG+ D RK+F H+ PNV+P +IV +TL++ Sbjct: 158 LSILQWPSLARIVRGEVLKLKNKEFMLAADILGISDRRKMFSHIFPNVLPQIIVFSTLSV 217 Query: 227 GGAILSESVLSFFGLGVMPPTPTWGNMIDAANNMIDFQDHPWLWIPPGLSIFATVIAINI 286 GG I+ ES LSF GLG+ P+ +WGNMI + F +PWLWIPPG+ IF TV++IN+ Sbjct: 218 GGNIMMESTLSFLGLGITEPSASWGNMIQVVGDFYTFDHYPWLWIPPGICIFLTVMSINL 277 Query: 287 FGDGLRDVLDPK 298 GD LR+ +D K Sbjct: 278 LGDSLREAVDTK 289 >BS_appC [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel transport systems, permease components Length = 303 Score = 222 bits (566), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 112/296 (37%), Positives = 173/296 (58%), Gaps = 7/296 (2%) Query: 6 EPQVQRQKSSLFRQSLKRLMKNRLXXXXXXXXXXMFALCFIGPFFSPYADNKINMAMMNK 65 + + ++ ++ + ++ KN+L + P +PY + ++ K Sbjct: 15 KENISKKPETMTKIFWEKFSKNKLAILGAVILFIIIMSAVFAPLIAPYPQEQQSLLDKYK 74 Query: 66 APSLQHLLGTDALGRDILTRVMQAGRISLTVGLASMVLSVFIGALLGAVAGYYRGIADQI 125 AP L+HL+GTD GRDI +R++ R+SL VG AS+V S+ IG +LGA+AGY+RGI D + Sbjct: 75 APGLEHLMGTDKFGRDIFSRILYGARVSLLVGFASVVGSILIGTVLGALAGYFRGIVDAV 134 Query: 126 IMRIADLLMTIPGLPLLFIFGALLSEWKVPTDYRMYIVMLMLSIVNWPGLARMVRGQMLS 185 IMR+ D++++IP + F+ L++ +K D ++L+ + W AR+VRG+ LS Sbjct: 135 IMRVVDIVLSIPDI---FLLITLVTIFKPGVDK----LILIFCLTGWTTTARLVRGEFLS 187 Query: 186 LREREFMQAAVVLGLRDSRKLFHHLLPNVVPLLIVMATLNIGGAILSESVLSFFGLGVMP 245 LR RE++ AA +G + + +F H+LPN + +IV ATL +G IL+ES LS+ G G+ P Sbjct: 188 LRSREYVLAAKTIGTKTHKIIFSHILPNALGPIIVSATLKVGSVILAESALSYLGFGIQP 247 Query: 246 PTPTWGNMIDAANNMIDFQDHPWLWIPPGLSIFATVIAINIFGDGLRDVLDPKQKR 301 P +WGNM+ A N W + PGL I TV+ N GDGLRD LDPK + Sbjct: 248 PIASWGNMLQDAQNFTVMIQAWWYPLFPGLFILMTVLCFNFVGDGLRDALDPKNIK 303 >BH0030 [EP] COG1173 ABC-type dipeptide/oligopeptide/nickel transport systems, permease components Length = 301 Score = 204 bits (518), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 120/305 (39%), Positives = 169/305 (55%), Gaps = 32/305 (10%) Query: 6 EPQVQRQKSSLFRQSLKRLMKNRLXXXXXXXXXXMFALCFIGPFFS---PYADNKINMAM 62 P++Q K+ K+L KN+ + IGP+F+ PY N + Sbjct: 20 NPRIQSWKNFY-----KKLAKNKAAMVGLSLIIFFIVVALIGPYFTTHDPYEPN-----V 69 Query: 63 MNK--APSLQHLLGTDALGRDILTRVMQAGRISLTVGLASMVLSVFIGALLGAVAGYYRG 120 MNK PS +H GTD GRDI TR++ I+L VG S+VL +G +LG V+GYY Sbjct: 70 MNKLAEPSAEHWFGTDHHGRDIFTRIIHGMSITLYVGFFSVVLGAIVGVILGVVSGYYGR 129 Query: 121 IADQIIMRIADLLMTIPG----LPLLFIFGALLSEWKVPTDYRMYIVMLMLSIVNWPGLA 176 + D +IMRI D+L+ PG L ++ I G ++ V++ + I + P A Sbjct: 130 LVDTVIMRIMDVLLAFPGILLALAIISILGGSINN-----------VIIAVGIFSIPVFA 178 Query: 177 RMVRGQMLSLREREFMQAAVVLGLRDSRKLFHHLLPNVVPLLIVMATLNIGGAILSESVL 236 R+VRG L++R+ E++ A LG D+R +F H+LPNV+ +IV TL+I +IL+ S L Sbjct: 179 RIVRGSTLAVRKLEYIDAVKALGASDARIIFKHILPNVLSPIIVQGTLSIATSILTASAL 238 Query: 237 SFFGLGVMPPTPTWGNMIDAANNMIDFQDHPWLWIPPGLSIFATVIAINIFGDGLRDVLD 296 SF GLG PP P WG M+ N + D P + + PGL+I V+A NIFGDGLRD LD Sbjct: 239 SFLGLGAQPPIPEWGAMLSDGRNYM--YDAPHVALFPGLAIVVVVLAFNIFGDGLRDALD 296 Query: 297 PKQKR 301 PK K Sbjct: 297 PKSKN 301 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,942,934 Number of extensions: 709534 Number of successful extensions: 4337 Number of sequences better than 1.0e-05: 212 Number of HSP's gapped: 4036 Number of HSP's successfully gapped: 215 Length of query: 301 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 195 Effective length of database: 26,153,027 Effective search space: 5099840265 Effective search space used: 5099840265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)