BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0009.p (714 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all4225_2 [T] COG2200 FOG: EAL domain 218 2e-56 all1175_4 [T] COG2200 FOG: EAL domain 215 2e-55 RSc0510_3 [T] COG2200 FOG: EAL domain 213 1e-54 all4897_4 [T] COG2200 FOG: EAL domain 211 3e-54 PA1727_3 [T] COG2200 FOG: EAL domain 210 6e-54 >all4225_2 [T] COG2200 FOG: EAL domain Length = 260 Score = 218 bits (556), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 103/243 (42%), Positives = 156/243 (64%), Gaps = 2/243 (0%) Query: 472 LHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGTFITAVEEAGLMIQL 531 LH ALER E V++YQP I+ T ++ +EALVRW HP +GL+ P FI E GL++++ Sbjct: 9 LHGALERNEIVVYYQPIIDLQTKQITAVEALVRWQHPTRGLVSPAKFIPIAEANGLIVEI 68 Query: 532 TDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAVLDQYGLETSMLEFE 591 +WVLR C Q W+ GLP +R S+N+S F NL+E + ++ + GL S LE E Sbjct: 69 GEWVLRTACLQNRTWQLAGLPPIRMSVNLSARQFEESNLVELVSEIIQESGLHPSYLELE 128 Query: 592 ITESTAL--LEEHYPLLQQMRDHGIVVSIDDFGTKYSSLNYLKHFPVNKIKIDRTFITGI 649 +TES+ + ++ +L+Q+R+ G+ +++DDFGT YSSLNYLK FPVN +KIDR+F+ + Sbjct: 129 VTESSLMTDIQRSVTILKQLRELGVWLALDDFGTGYSSLNYLKRFPVNMLKIDRSFVQDV 188 Query: 650 GVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHIQGFLFFPPLPAEDI 709 + + +I+ +A L + AEG+ETQ+QL +L K C QG+ F P PA++I Sbjct: 189 CSNPDSAAVTNAIIALAKSLQLKITAEGIETQQQLSYLQKRGCQEGQGYYFGIPAPAKEI 248 Query: 710 HKL 712 ++ Sbjct: 249 MEI 251 >all1175_4 [T] COG2200 FOG: EAL domain Length = 261 Score = 215 bits (547), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 107/242 (44%), Positives = 156/242 (64%), Gaps = 2/242 (0%) Query: 468 IAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGTFITAVEEAGL 527 + K L ALE++E ++YQP++N +TG++ GMEAL+RW HPE G++ P FI EE GL Sbjct: 5 LEKSLRYALEKQELKVYYQPRVNILTGQITGMEALLRWQHPEMGIVAPSIFIPIAEETGL 64 Query: 528 MIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAVLDQYGLETSM 587 +I + +WVLR C Q W+ GLP ++ ++N+S F L+E + A+L + GL Sbjct: 65 IIPIGEWVLRTACMQNRAWQEAGLPPVQMAVNLSPKQFRQPTLLELVSAILAETGLAPEF 124 Query: 588 LEFEITESTALLEEHY--PLLQQMRDHGIVVSIDDFGTKYSSLNYLKHFPVNKIKIDRTF 645 LE EITESTA+ + + +L+ ++ G+ ++IDDFGT +SSL+ L+ P++ +KID +F Sbjct: 125 LELEITESTAIEDIDFTRSVLENLQQMGVDIAIDDFGTGHSSLSRLQLLPLHNLKIDGSF 184 Query: 646 ITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHIQGFLFFPPLP 705 I + S I+K+IV + LG L AEGVE QE+LDFL NC +QGFLF PL Sbjct: 185 IKALTTDSKVAHIVKAIVTLGRSLGLRLTAEGVEKQEELDFLKSINCQDVQGFLFHKPLS 244 Query: 706 AE 707 AE Sbjct: 245 AE 246 >RSc0510_3 [T] COG2200 FOG: EAL domain Length = 282 Score = 213 bits (541), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 106/261 (40%), Positives = 155/261 (59%), Gaps = 2/261 (0%) Query: 451 YFIFDWGLREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEK 510 Y F+ + + Q+ + + L ALER E VLHYQP+ +A G ++G+EALVRW HPE+ Sbjct: 1 YCFFEASMNTNVHQQLQLVQDLRLALERHELVLHYQPKFSAPHGPVVGVEALVRWQHPER 60 Query: 511 GLIGPGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNL 570 GL+ P FI E+ GL++ L WVL E CRQ+ +WR G ++N+S F + L Sbjct: 61 GLVQPDAFIPLAEKTGLIVPLGAWVLDEACRQMAQWRREGHAQWSIAVNLSAVQFAHAAL 120 Query: 571 IENLLAVLDQYGLETSMLEFEITESTALLEEHYPL--LQQMRDHGIVVSIDDFGTKYSSL 628 ++ + L ++ LE L EITESTA+ + L LQQ+ G+ +SIDDFGT YSSL Sbjct: 121 VDTVRDTLARHALEPQGLTLEITESTAMRDADASLHILQQLHAMGVRISIDDFGTGYSSL 180 Query: 629 NYLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLI 688 YLK P +++KIDR F+ + S D I+ ++V + L ++AEGVET+ Q FL Sbjct: 181 LYLKRLPASELKIDRGFVRDLAHDSEDAAIVSAVVALGQTLNLRIVAEGVETEAQQAFLT 240 Query: 689 KHNCPHIQGFLFFPPLPAEDI 709 + C +QG L P+ A+ + Sbjct: 241 RLGCHALQGNLLGRPMTAQAV 261 >all4897_4 [T] COG2200 FOG: EAL domain Length = 272 Score = 211 bits (537), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 112/263 (42%), Positives = 159/263 (60%), Gaps = 6/263 (2%) Query: 455 DWGLREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIG 514 D+ + + Q + L A+ER+EF +HYQP ++ T K+IG EALVRW HP+ GL+ Sbjct: 1 DFSMYSQAAQLLQLEMDLRRAVERQEFQVHYQPIVSLETYKIIGFEALVRWQHPQHGLVS 60 Query: 515 PGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRT--MGLPLLRTSINISNSWFYNRNLIE 572 P FI+ EE GL++ + WVLRE CRQ+ W+ P L S+NIS+ F NLIE Sbjct: 61 PERFISLAEETGLIVPIGYWVLREACRQMRAWQLEFTTHPPLTISVNISSKQFSQPNLIE 120 Query: 573 NLLAVLDQYGLETSMLEFEITESTALLEEH---YPLLQQMRDHGIVVSIDDFGTKYSSLN 629 + +L GLE S L+ EITE T L+E H +L Q++ I + +DDFG YSSL+ Sbjct: 121 EIRKILQDTGLEASSLKLEITE-TVLMENHESATAMLLQLQQMNIQLHLDDFGIGYSSLS 179 Query: 630 YLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIK 689 YL FP + +KIDR+F+ +G + I+++I+ +A L D+IAEGVET EQL L Sbjct: 180 YLHRFPSSALKIDRSFVINLGADEKNLEIVQAIISLALSLNIDVIAEGVETMEQLRQLQI 239 Query: 690 HNCPHIQGFLFFPPLPAEDIHKL 712 C H QG++F PL ++ + L Sbjct: 240 KKCKHAQGYIFSQPLDSQSVDTL 262 >PA1727_3 [T] COG2200 FOG: EAL domain Length = 254 Score = 210 bits (535), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 106/240 (44%), Positives = 151/240 (62%), Gaps = 2/240 (0%) Query: 472 LHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGTFITAVEEAGLMIQL 531 L ALER + VLHYQP++ A G MIG+EAL+RW HP+ GLI PG F+ E+ GL++Q+ Sbjct: 10 LRQALERRQLVLHYQPKVLAPNGPMIGVEALLRWEHPQHGLITPGQFLPLAEKTGLIVQI 69 Query: 532 TDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAVLDQYGLETSMLEFE 591 +WVL E CRQ+ W G ++N+S F + L++++ L ++ LE S L E Sbjct: 70 GEWVLDEACRQMRLWLDGGHADWNIAVNLSALQFAHAGLVDSVRNALLRHSLEPSHLILE 129 Query: 592 ITESTALLEEHYPL--LQQMRDHGIVVSIDDFGTKYSSLNYLKHFPVNKIKIDRTFITGI 649 +TESTA+ + L L+Q+ G+ +SIDDFGT YSSL YLK P +++KIDR FI + Sbjct: 130 VTESTAMRDADASLVILEQLSAMGVGISIDDFGTGYSSLLYLKRLPASELKIDRGFINEL 189 Query: 650 GVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHIQGFLFFPPLPAEDI 709 S D I+ +IV + L ++AEGVET+ Q +FL + C +QGFL P+PAE + Sbjct: 190 AHDSDDAAIVSAIVALGRTLNLKIVAEGVETEAQQEFLTRLGCNSLQGFLLGRPMPAEQL 249 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 49,381,247 Number of extensions: 2074124 Number of successful extensions: 4671 Number of sequences better than 1.0e-05: 261 Number of HSP's gapped: 4314 Number of HSP's successfully gapped: 262 Length of query: 714 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 600 Effective length of database: 25,230,355 Effective search space: 15138213000 Effective search space used: 15138213000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)