BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0004.p (455 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VCA0088 [C] COG1301 Na+/H+-dicarboxylate symporters 228 1e-59 XF0656 [C] COG1301 Na+/H+-dicarboxylate symporters 227 4e-59 BH3820 [C] COG1301 Na+/H+-dicarboxylate symporters 210 4e-54 RC0223 [C] COG1301 Na+/H+-dicarboxylate symporters 197 3e-50 CT401 [C] COG1301 Na+/H+-dicarboxylate symporters 194 2e-49 >VCA0088 [C] COG1301 Na+/H+-dicarboxylate symporters Length = 424 Score = 228 bits (581), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 127/373 (34%), Positives = 194/373 (52%), Gaps = 5/373 (1%) Query: 71 GSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGLSVAL 130 G I+++ ++MLV+PLVF+ ++ SSL +L L ++G KT ++L TT +A + L++ Sbjct: 53 GKIFIASLKMLVVPLVFVSLVCGTSSLKDLSTLGRMGSKTLGFYLATTAIAITLALTMGS 112 Query: 131 LFNPGKGIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXXXXXXXXXXXX 190 LF PG G F + E P+ +VI+D+ P+NP++ A G + Sbjct: 113 LFQPGAGADLTAASNFTSAEAPSLGKVIVDMFPTNPISAMAQGNTLQIIIFAVLFGVAIS 172 Query: 191 KVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARYGWETLQELGS 250 G + DL E V+ ++V ++ L PYGVF L+A + G + L Sbjct: 173 AAGKPGERIAQVFNDLNE----VIMKLVAMLMHLAPYGVFFLMAKLFTGLGLGAILNLAE 228 Query: 251 VIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSYATLPVNLEVI 310 + L++H A +NP+ FFRK+ + AF+T SS AT+PV +E + Sbjct: 229 YFVVLAGTLLLHAFVTYGLMLKTFAGLNPMVFFRKMEDAIMFAFSTASSNATIPVTMETV 288 Query: 311 TKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTDYVTLVLVSII 370 RL V ++ASF PLGA+VN +G I + F AQ F I LT TDY+ ++L + + Sbjct: 289 KNRLGVDNKVASFTVPLGATVNMDGT-AIMQGVATAFIAQAFNIDLTMTDYLMVILTATL 347 Query: 371 SSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGRXXXXXXXXXXXXXXXX 430 +S+G AGVPG ++ +VL +GLPLEGIA++ GV+ L+DM R Sbjct: 348 ASVGTAGVPGVGLVMLAMVLNQVGLPLEGIALIMGVDRLLDMIRTAVNITGDACVSCIVA 407 Query: 431 XSEGEFDREAFNR 443 SE FD F R Sbjct: 408 KSENAFDERTFCR 420 >XF0656 [C] COG1301 Na+/H+-dicarboxylate symporters Length = 437 Score = 227 bits (578), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 131/362 (36%), Positives = 196/362 (54%), Gaps = 23/362 (6%) Query: 71 GSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGLSVAL 130 G++YV+LI+M+ IPLVF V+N+I++L + +G +TF WF+ T +A +GL V Sbjct: 42 GTLYVTLIKMIAIPLVFFAVINAIAALHGQRSVAMLGGRTFLWFIVTAALAVCVGLGVGT 101 Query: 131 LFNPGKG-IQQAVPEGFEAREIPTFSQVILDLVPSNPVNE-----------------AAT 172 L PG G +V + E+P+ V+LD+VPSNP A Sbjct: 102 LLKPGNGDFTVSVDAAWAPPEVPSPVHVLLDVVPSNPFYALTGIGTRSNSTGETTLAAGR 161 Query: 173 GKVVPXXXXXXXXXXXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFAL 232 G ++P K+G E V VR LI ++ ++ Q+ +FV+ +TP G F L Sbjct: 162 GSILPVIFFAALLGLAIVKLG----ERVTEVRKLIGQMSEIMIQITRFVLEMTPLGTFGL 217 Query: 233 IAGITARYGWETLQELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTV 292 IA + YG++ L G ++ Y+A H ++PL+FFR + P + V Sbjct: 218 IATLVGSYGFQKLLPFGHFVLALYLACAFHIVVVYSGLLLAHG-LSPLKFFRGVAPGMQV 276 Query: 293 AFTTRSSYATLPVNLEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVF 352 AF + SS+A +PV L IT L V+ A+F PLG+S+ +GCG I+PA+ AVF AQ Sbjct: 277 AFVSSSSFAAMPVALRAITHNLGVNKDYAAFAVPLGSSIKMDGCGAIFPALCAVFIAQYT 336 Query: 353 GIHLTGTDYVTLVLVSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDM 412 G+ LT Y + + S++ S G AGVPG AV+ TVVL+A LPLE I + ++ ++DM Sbjct: 337 GVPLTANQYFVIFIASVLGSFGTAGVPGTAVVMATVVLSAAHLPLEVIGYLYAIDRVLDM 396 Query: 413 GR 414 R Sbjct: 397 MR 398 >BH3820 [C] COG1301 Na+/H+-dicarboxylate symporters Length = 413 Score = 210 bits (534), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 127/354 (35%), Positives = 202/354 (57%), Gaps = 6/354 (1%) Query: 62 LETEAIKTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVA 121 ++T G +++ LI MLV+PLV ++ + L + + L K+G KT ++L TT +A Sbjct: 32 IDTYVFTPVGQLFLRLIMMLVVPLVLFSIILGTAGLGDPKKLGKLGSKTVGFYLLTTMIA 91 Query: 122 SIIGLSVALLFNPGK-GIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXX 180 +IGLS+A + PG+ G+ V E FEA E P + IL+++P NP+ A+G+++ Sbjct: 92 LVIGLSIAYVLQPGEPGLMGTVEEEFEATEAPPVMETILNIIPRNPIEALASGQMLQIIA 151 Query: 181 XXXXXXXXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARY 240 ++G K +K L+E V+ +V ++L PYG F LIA + Sbjct: 152 FAVLVGLALGRLGEKTSGILK----LVEQGNEVMMYLVSLAMKLAPYGAFGLIASALGKL 207 Query: 241 GWETLQELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSY 300 G++ L + +I +AL++H AK NPL F R +P + +AF T SS Sbjct: 208 GFDALGSMAMYVIAVLLALLLHAVITYGGFLAFVAKRNPLEFLRNFFPAMALAFGTSSSS 267 Query: 301 ATLPVNLEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTD 360 A LPV+++ ++L VS ++SFV PLGA++N +G I A+ VF AQV+ L+ D Sbjct: 268 AVLPVSMKTAQEKLGVSKPVSSFVQPLGATINMDGT-AIMQAVATVFIAQVYAQQLSMGD 326 Query: 361 YVTLVLVSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGR 414 +T+VL + ++SIG AGVPG +I +VL ++GLP+EGIA+V V+ ++DM R Sbjct: 327 LLTVVLTATLASIGTAGVPGVGLIMLAMVLQSIGLPVEGIALVLAVDRILDMSR 380 >RC0223 [C] COG1301 Na+/H+-dicarboxylate symporters Length = 399 Score = 197 bits (500), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 120/376 (31%), Positives = 201/376 (53%), Gaps = 7/376 (1%) Query: 67 IKTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGL 126 IK G I++ LI+M++ PL+F +++ I+S+ + L ++G+K A FLGTT A++ GL Sbjct: 28 IKPIGDIFLRLIKMIITPLIFFSLVSGITSMNDNSALGRVGMKAVAAFLGTTFFATVFGL 87 Query: 127 SVALLFNPGKGIQ-QAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXXXXXXX 185 +VAL+ PG G+ G R +++VP N V A G V+ Sbjct: 88 TVALVLKPGVGVHIDFTSSGTTNRTSFNIIDFFVNIVPDNAVGAFANGDVLQVVFFAIFV 147 Query: 186 XXXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARYGWETL 245 K+ S +P+ DLI ++ ++ +++ FVI+L+PYG FAL I G E + Sbjct: 148 GITLNKMKSIG----EPITDLIHVMSKLILKMISFVIQLSPYGAFALTGWIVGMQGVEVM 203 Query: 246 QELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSYATLPV 305 L +++ VA+ +V+P+ F++K + +AF+T SS ATL Sbjct: 204 ISLSKLVVAVVVAMTFQYLVFGLLICVF-CRVSPIPFYKKSFEYQILAFSTSSSKATLAT 262 Query: 306 NLEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTDYVTLV 365 ++V +L +S SFV P+GAS+N +G I ++ +F AQ+ G+ L DY+ ++ Sbjct: 263 TMQVCRAKLGISESSTSFVLPIGASINMDGFA-INLSLTTIFFAQMMGVTLAPHDYLVII 321 Query: 366 LVSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGRXXXXXXXXXXX 425 L S + SIG AG+PG ++I +VL+++ LP+EG+AI+AG++ ++DM R Sbjct: 322 LTSTLGSIGGAGIPGASLIMLPMVLSSVHLPIEGVAIIAGIDRILDMLRTTINITGDATI 381 Query: 426 XXXXXXSEGEFDREAF 441 SE D+E + Sbjct: 382 TMIIDNSEDTLDKEVY 397 >CT401 [C] COG1301 Na+/H+-dicarboxylate symporters Length = 412 Score = 194 bits (494), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 129/400 (32%), Positives = 208/400 (52%), Gaps = 28/400 (7%) Query: 62 LETEAI--KTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTG 119 LE +A+ K FG I+++L+ M+V PLVF ++ I+S+++++ L +IG+++ +L TT Sbjct: 21 LEDKAVFVKPFGDIFLNLLSMVVYPLVFCSMVLGIASISDMKKLGRIGLRSLGLYLATTS 80 Query: 120 VASIIGLSVALLFNPGKGIQQAVPEGFEARE------------IPTFSQVILDLVPSNPV 167 VA IGL+ AL F+PGKG +GF A + + TF+QV PSNP+ Sbjct: 81 VAIGIGLAFALFFSPGKGCDL---QGFNAVDLASSGSSGGMNFLATFAQVF----PSNPL 133 Query: 168 NEAATGKVVPXXXXXXXXXXXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPY 227 G ++ G EA +PV + ++ ++V ++ PY Sbjct: 134 RSFVEGNILQIIIFALFTGIAMRLAG----EAARPVERFVHGCNEIMLRMVNMIMSFAPY 189 Query: 228 GVFALIAGITARYGWETLQELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIY 287 GV A +A I+ +G L +LG ++ Y+A I H + + +F + Sbjct: 190 GVAASMAWISGNHGLTVLLQLGKFLVVYYLACICHAILIFGGLVRLGCRSSFSKFLSSMM 249 Query: 288 PTLTVAFTTRSSYATLPVNLEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVF 347 ++ A +T SS ATLPV + ++K L VS ++ FV PLGA+VN NG I+ + AVF Sbjct: 250 DAISCAVSTASSSATLPVTMRCVSKNLGVSSEVSGFVLPLGATVNMNGT-AIFQGMAAVF 308 Query: 348 TAQVFGIHLTGTDYVTLVLVSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVE 407 AQ + L+ T + LV+ + S++G AGVPG +I+ VLT++GLP++GIA++AG++ Sbjct: 309 IAQAYNCPLSLTSLLLLVVTATFSAVGSAGVPGGGMITLGSVLTSVGLPIQGIAVLAGID 368 Query: 408 ALIDMGRXXXXXXXXXXXXXXXXXSEGEFDREAFNRGDDD 447 L D+ EGEF N G++D Sbjct: 369 RLRDIIGTPMNILGDAVVALYIADGEGEFSSS--NEGEED 406 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.141 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,173,213 Number of extensions: 1059256 Number of successful extensions: 2354 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 2201 Number of HSP's successfully gapped: 55 Length of query: 455 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 345 Effective length of database: 25,691,691 Effective search space: 8863633395 Effective search space used: 8863633395 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 117 (49.7 bits)